Method for removing saralytic saxitoxin

A shellfish toxin and paralytic technology, which is applied in the field of removing paralytic shellfish toxin in water by embedding adsorption technology, which can solve the problems of lack of pertinence and no significant effect.

Inactive Publication Date: 2009-02-18
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods lack specifici

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1. Prepare 50ml of sodium alginate solution with a concentration of 0.5%. After fully dissolving, add 5% activated carbon powder activated at 110° C. and stir well. Then use a medical syringe to absorb the mixed solution and pour it into a calcium chloride solution with a concentration of 2%, and place it for 1 hour to obtain solidified alginate-embedded activated carbon pellets (embedded adsorbent). The paralytic shellfish toxin was extracted from poisonous snails according to the method of RIDASCREEN Saxitoxin kit to obtain the poisonous supernatant. Take 10 ml of the sample supernatant, add 5 g of the above-mentioned embedded adsorbent, stir for 5-30 minutes, and remove the adsorbent by filtration. Use a microplate reader to detect the content of paralytic shellfish toxin before and after treatment, according to the formula: detoxification rate%=(untreated toxin content-treated toxin content)×100% / untreated toxin content, calculate the detoxification rate ...

Embodiment 2

[0016] Embodiment two. Prepare 50ml of 0.5% carrageenan solution, after fully dissolving, add 5% active carbon powder activated through 110 ℃ and fully stir. Pour into a watch glass to cool and solidify, and cut the solidified colloid into strips or particles of uniform size. The bar was used to treat the toxic solution as in Example 1, and the detoxification rate was measured to be 75.8%.

Embodiment 3

[0017] Example 3. Prepare 1.5% sodium alginate solution with 0.025M, pH6.5 phosphate buffer. Bought mature bullfrogs from the market, extracted plasma from bullfrog abdominal aorta, added anticoagulant heparin sodium solution and 1um pepsin and aprotinin in a ratio of 10%, to inhibit protein denaturation; centrifuged at 4000rmp, 4°C After 30 minutes, the crude supernatant containing Saxiphilin protein was obtained. After being concentrated by centrifugal ultrafiltration, the crude protein and sodium alginate solution are mixed evenly. Draw the mixed solution with a medical syringe, inject it dropwise into a calcium chloride solution with a concentration of 2%, and leave it for 1 hour to obtain protein-embedded gel beads, and then treat the toxin-containing extract as in Example 1. The toxin removal rate is over 90.0%.

[0018] In addition to conventional adsorbents, the method of the present invention uses bioactive ingredients that can combine with paralytic shellfish toxin...

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PUM

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Abstract

The invention provides a removal method of paralytic shellfish poison, comprising the following steps: firstly, absorbent is distributed in embedding medium evenly; secondly, the embedding medium which contains the absorbent is solidified to form gel; thirdly, the solidified embedding medium which contains the absorbent is made into gel balls or blocks with uniform size; fourthly, the balls or the blocks are put in the solution which contains the paralytic shellfish poison, and the solution is then stirred for 5-30 minutes; and lastly, the gel is removed by filtration or centrifuge method to get the water which has removed the paralytic shellfish poison. The invention has the advantages that the absorbent which is treated by embedding can absorb the paralytic shellfish poison in the water. The absorption efficiency is higher than both pure absorbent and embedding medium. The embedded absorbent is comparatively big grain which is convenient to be separated from the system. The method can be used to remove the paralytic shellfish poison in the water, can be used for the enrichment and detection of positions, the purification of shellfish and so on, and can be used for the purification of other positions and pollutants in the environment after being improved.

Description

technical field [0001] The invention relates to a method for removing paralytic shellfish toxins, in particular to a method for removing paralytic shellfish toxins in water bodies by using embedment adsorption technology. Background technique [0002] Shellfish toxins are harmful organic substances accumulated in shellfish. They are some highly toxic non-protein toxins. They are the most toxic substances in nature. They cannot be inactivated by heating at high temperatures. After eating, they can cause poisoning and even death. . There are many types of shellfish toxins, four of which have been found so far, among which the most harmful to humans, the most studied is paralytic shellfish poisoning (PSP: Paralytic shellfish poisoning). [0003] At present, it is generally believed that the non-toxic edible shellfish is temporarily poisoned, which is caused by the poisoning of the external environment. The poisoning is caused by the poisonous algae containing the paralytic tox...

Claims

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Application Information

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IPC IPC(8): C02F1/58C02F9/02C02F1/28C02F1/38
Inventor 欧昌荣苏秀榕汤海青何仲
Owner NINGBO UNIV
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