Preparation of bisphenol A submicron magnetic molecular imprint and application of same in detection pre-processing of packaged food

A magnetic molecular imprinting and molecular imprinting technology, applied in the fields of material chemistry and food safety, can solve the problems of cumbersome operation steps, low adsorption efficiency, large consumption of organic reagents, etc., to fill the application gap, high adsorption efficiency, and large specific surface area. Effect

Inactive Publication Date: 2012-01-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used pretreatment methods include liquid-liquid extraction (LLE) and solid phase extraction (SPE) methods, but these two methods have the following disadvantages: the consumption of organic reagents is large; the operation steps are cumbersome; time-consuming; there is no selective extraction for BPA sex
However, the specific surface area of ​​molecularly imprinted microspheres is relatively small, and the adsorption efficiency is low, and the application of submicron magnetic molecularly imprinted particles in actual sample detection is still blank.

Method used

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  • Preparation of bisphenol A submicron magnetic molecular imprint and application of same in detection pre-processing of packaged food
  • Preparation of bisphenol A submicron magnetic molecular imprint and application of same in detection pre-processing of packaged food
  • Preparation of bisphenol A submicron magnetic molecular imprint and application of same in detection pre-processing of packaged food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of Bisphenol A Submicron Magnetic Molecular Imprint

[0032] (1) Magnetic submicron materials (Fe 3 o 4 )Synthesis.

[0033] Accurately weigh 2 g of ferric chloride hexahydrate and 4.8 g of sodium acetate and dissolve in 60 mL of ethylene glycol, then add 1.5 g of polyethylene glycol and fully stir for 2 h under the action of a mechanical stirrer, transfer all the yellow liquid to the In a closed stainless steel hydrothermal reaction kettle with a polytetrafluoroethylene liner (100 mL), heat the reaction in an oil bath at 210 °C for 12 h. After the reaction was completed, the obtained black substance was repeatedly washed 6 times with absolute ethanol, and then vacuum-dried at room temperature to obtain the magnetic submicron material Fe 3 o 4 .

[0034] (2) Silicon-coated magnetism (Fe 3 o 4 SiO 2 ) synthesis of microspheres.

[0035] Accurately weigh 0.3g Fe 3 o 4 (particle size about 150 nm) was dissolved in 0.1M hydrochloric acid (10...

Embodiment 2

[0040] Example 2 Pretreatment of canned orange samples for detection

[0041] Use a pipette to accurately draw 20 mL of broken canned orange samples and place them in a 50 mL centrifuge tube, dilute to 30 mL with PBS buffer solution of pH 8.9, mix with a shaker for 1 minute, and centrifuge at 7500 rpm for 5 minutes. The supernatant was collected; the precipitate was eluted twice with 2 mL of methanol, the eluate was combined with the supernatant, and diluted to 50 mL with PBS buffer of pH 8.9. Accurately weigh the dried molecularly imprinted magnetic submicron particles Fe 3 o 4 Disperse 15mg of MIP into the diluent, mix well on the mixer for 2min, make the magnetic submicron particles gather on the wall of the centrifuge tube under the action of a rare earth magnet, discard the solution, and rinse the magnetic submicron particles with 2 mL ultrapure water , discard the eluate, and finally elute with 2 mL of methanol (containing 0.6%) acetic acid, collect the eluate and ...

Embodiment 3

[0045] Example 3 Pretreatment of packaged pure milk samples for detection

[0046] Use a pipette to accurately draw 20 mL of milk sample into a 50 mL centrifuge tube, dilute to 30 mL with pH 8.9 PBS buffer, add 500 μL of zinc acetate (1M) and potassium ferrocyanide (0.2M) respectively, shake After mixing for 1 minute, centrifuge at 7500 rpm for 5 minutes to collect the supernatant; the precipitate was eluted twice with 2 mL of methanol, the eluate was combined with the supernatant, and diluted to 50 mL with PBS buffer at pH 8.9. Accurately weigh the dried molecularly imprinted magnetic submicron particles Fe 3 o 4 Disperse 15mg of MIP into the diluent, mix thoroughly on the mixer for 2min, and gather the magnetic submicron particles on the wall of the centrifuge tube under the action of a rare earth magnet, discard the solution, and rinse the magnetic submicron particles with 2 mL ultrapure water , discard the eluent, and finally elute with 2 mL of methanol (containing 0.6%)...

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Abstract

The invention provides a preparation of a bisphenol A submicron magnetic molecular imprint and application of the same in the detection pre-processing of packaged food, which belong to the technical field of material chemistry and food safety. A surface molecular imprinting technology and a magnetic solid-phase extraction technology are combined, and a magnetic submicron particle wrapped by a surface molecular imprint polymer (Fe3O4@MIP) is prepared by using a bisphenol A as a template molecule. A magnetic submicron material (Fe3O4) is synthesized by a hydrothermal process, the synthesized Fe3O4 is synthesized into a silicon-wrapped magnetic microsphere (Fe3O4@SiO2) by a sol gel process, the synthesized Fe3O4@SiO2 is coupled with 2-bromo-isobutyryl bromide to form Fe3O4@SiO2@Br with Br groups on the surface, and the synthesized Fe3O4@SiO2@Br is synthesized into a submicron particle with a surface molecular imprint (Fe3O4@MIP) by an atom radical transfer process. The prepared Fe3O4@MIP is used in the detection pre-processing of packaged food which comprises liquid food, liquid food containing a certain quantity of solids and solid food.

Description

technical field [0001] The preparation of a submicron magnetic molecular imprint of bisphenol A and its application in the pre-treatment of packaged food detection belong to the technical field of material chemistry and food safety. Background technique [0002] In recent years, the potential harm caused by long-term exposure to exogenous interfering substances has attracted great attention from the scientific community and the public. Among the exogenous interfering substances, bisphenol A (BPA) is one of the main hazards. Bisphenol A is the main raw material for the production of polycarbonate and epoxy resin, and is also used in phenolic resin, plastic polyester, antioxidant and polyvinyl chloride (PVC) stabilizer, etc., in food packaging materials, container inner wall coatings, etc. has important uses. Bisphenol A penetrates into the food through the liquid components in the packaged food from the food packaging, seriously endangering human health. [0003] Curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08J9/28H01F1/00B01J20/26B01J20/30G01N30/08
Inventor 王利兵胥传来许宙丁利焦艳娜徐丽广陈练
Owner JIANGNAN UNIV
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