The invention also discloses application of three-dimensional fluorescence spectrometry in identification of paralytic shellfish toxin-producing microalgae

Abstract

The invention discloses an application of a three-dimensional fluorescence spectrometry in identification of paralytic shellfish toxin-producing microalgae. The method comprises the following steps: extracting three-dimensional fluorescence spectrum information of PSP-producing algae and PSP-non-producing algae growing under different environmental conditions, extracting characteristic peaks of experimental algae by using a Coif2 wavelet function, performing clustering analysis by using a system clustering method, removing abnormal spectrums, and screening standard spectrums to obtain a Coif2fluorescence characteristic standard spectrum library; when the discriminating function established according to the fluorescence characteristic standard spectrum library is used for discriminating the PSP-producing algae and the PSP-non-producing algae, the discriminating correct rates are respectively 77.3% and 84.5%, the correct rates are very high, and the purpose of quickly and accurately identifying the toxin-producing algae is basically achieved. The invention provides a research result of the application of the three-dimensional fluorescence spectrometry in identification of paralyticshellfish toxin-producing microalgae for the first time, and the three-dimensional fluorescence spectrometry can be applied to the aspects of red tide algae identification sensors or portable algae fluorescence identification instruments and the like.

Description

technical field [0001] The invention belongs to the field of identification and prevention of toxic microalgae, and in particular relates to the application of three-dimensional fluorescence spectroscopy in identification of paralytic shellfish toxin-producing microalgae. Background technique [0002] Red tide refers to the phenomenon that the microorganisms in the water multiply due to the water environment and climate, resulting in the discoloration of the water body. In recent years, the frequency and area of ​​red tides in my country's coastal areas have continued to increase, causing serious economic losses. [0003] According to the toxicity characteristics of red tides, red tides are usually divided into three categories, namely non-toxic red tides, fish toxic red tides and toxic red tides. Among the three types of red tides, the proportion of non-toxic red tides is the highest. This type of red tide does not produce toxins. The toxins produced by fish-toxic red tide...

AI Technical Summary

Problems solved by technology

[0007] There are some methods for detecting PSP, such as biological detection, immunoassay, and chemical analysis, but these methods have defects in accuracy, sensitivity, and poor repeatability, or require large and expensive equipment, professional operators, and complicated and time-sensitive sample pretreatment. However, it cannot be widely used in the monitoring and management of shellfish toxins

In addition, there is currently no toxicity detection technology available for living cells of PSP-producing algae, which seriously restricts the on-site management of shellfish toxins

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