The invention discloses a wide-view-field chromatography hyperspectral microscopic imaging method and device based on space-time focusing, and belongs to the technical field of microscopic spectral imaging and analytical chemistry. According to the method, ultra-short pulse lasers are generated through an ultra-short pulse laser source, focusing lines are generated in a sample through space-time focusing, excited fluorescence is collected, stray light is filtered out through con-focal optical slits, spectral information (x, lambda), of the sample is acquired through fluorescence spectral information collecting, and finally sample (x, lambda, y, z, t) five-dimensional information is acquired through three-dimensional space scanning and delayed scanning. The device comprises the ultra-shortpulse laser source, a light beam conversion system, a line scanning system based on space-time focusing, an optical microscopic system and a filtering and synchronous spectrum con-focal detection system. Acquisition of the spectral information in the filtering and synchronous spectrum con-focal detection system is synchronous with line scanning trigger signals in the line scanning system in combination with the space-time focusing technology. The method and device have the advantages of being wide in view field, high in space resolution, high in time resolution, high in spectral resolution andthe like.