13 results about "Bio imaging" patented technology

This invention provides a long-afterglow compound, system, preparation method, and application based on covalent bond coupling. The long-afterglow compound absorbs photons and transfers the absorbed photon energy within the molecule, undergoing a photochemical reaction that releases the energy as light, thus achieving afterglow luminescence. The structure of the long-afterglow compound is shown in Formula I, where X is selected from O or S; R is selected from a photosensitizer; and n is 1–15. The long-afterglow system includes a carrier medium and a long-afterglow compound, wherein the carrier medium is used to dissolve, disperse, or adsorb the long-afterglow compound. This invention can achieve near-infrared luminescence at 660 nm and 718 nm, and can be applied in fields such as bioimaging, immune detection, cell tracking, biosensing, and information encryption.

The present invention relates to compounds of Formula I, in which Y, Ar1, Ar2, X, R1, and R2 are defined herein, and to their use in various biological imaging techniques and therapeutic methods. The present invention relates, in aspects, to conjugates comprising compounds of Formula I and their related uses and therapeutic uses.

This invention discloses a method for preparing a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film, comprising: reacting a terminal amino-substituted alkane with halogen-modified naphthalenecarboxylic anhydride to synthesize an organic light-emitting small molecule with phosphorescent / fluorescent dual-emission function; and mixing the obtained organic light-emitting small molecule into a dynamically cross-linked supramolecular polymer to obtain a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film. This invention also discloses the single-component room-temperature phosphorescent / fluorescent dual-emission polymer film prepared by the above method and its applications. The preparation method of this invention is simple and low-cost. The polymer film prepared with only a single component can generate a phosphorescent / fluorescent dual-emission ratio light signal with stable emission. The film color changes with changes in ambient humidity, and the film can sense ambient humidity in the range of 1–100%, showing broad application prospects in sensing, anti-counterfeiting, and bioimaging fields.

This invention relates to a lysosome-targeting fluorescent dye based on the BODIPY structure and its synthesis method, belonging to the field of organic fluorescent dyes. The invention designs and synthesizes the lysosome-targeting fluorescent dye BDP. The dye molecule uses BODIPY as the parent structure, and a novel lysosome-targeting fluorescent dye molecule is synthesized by introducing diphenylphosphine salt and tert-butyloxycarbonyl groups. The synthetic route of this dye is simple, it possesses excellent photostability, and exhibits good resistance to interference from ions, amino acids, and viscosity. Furthermore, this dye demonstrates superior fluorescence performance in a variety of common solvents, with fluorescence quantum yields exceeding 0.70. Experimental results show that this fluorescent dye can precisely target lysosomes in living cells, demonstrating promising applications in the field of bioimaging.

The application discloses a preparation method of a gallium germanate near-infrared luminescent material, and the luminescent material has a chemical formula of Ga 3+x‑y Al 3‑ x Ge2O 13 : y Cr 3+ According to a stoichiometric ratio of each raw material in the chemical formula, each raw material is weighed, Ga is introduced through an oxide, Al is introduced through an oxide or a hydroxide, Ge is introduced through an oxide, and Cr is introduced through an oxide or a nitrate; the raw materials are fully mixed and ground to obtain a raw material powder; the raw material powder is kept at a certain temperature in an air atmosphere, is cooled to room temperature in a furnace, is ground, and the near-infrared luminescent material is prepared. The preparation method can obtain the near-infrared luminescent material which has the advantages of a spectrum peak greater than 800 nm, adjustable spectrum, high luminescent efficiency (an external quantum efficiency is greater than 30%), good thermal stability (a residual luminescent intensity at 150 o C is greater than 60% at room temperature), can be efficiently excited by a blue light chip, and the like, can be applied to biological imaging, plant illumination, night monitoring and food detection, the preparation method is simple, no waste water and waste gas are discharged, and the preparation method is suitable for large-scale industrial production.

This invention relates to a low-dose aggregation-induced emission dye (AIE) bioimaging method, comprising the following steps: selecting a test organism and injecting it with a low dose of AIE; stimulating the test organism with an adjustable frequency excitation light source; acquiring fluorescence signals emitted from the test organism at equal time intervals; processing the fluorescence signals and generating a fluorescence image; applying phase-locked processing to the fluorescence image to separate the fluorescence signal component with the same frequency as the excitation light source and generating a phase-locked imaging result. By convolving the amplitude signal of each pixel in the fluorescence image with a sine or cosine signal with the same frequency as the excitation source, the orthogonality of trigonometric functions is utilized to effectively suppress noise, thus preserving the fluorescence signal that changes with the same frequency as the periodic light source. This significantly improves the signal-to-noise ratio of the fluorescence image, enabling clear observation of the AIE fluorescence signal even at low doses.

The present application belongs to the technical field of image processing, and particularly relates to a plant microtubule image super-resolution reconstruction method and system based on quantum inspiration. The method of the present application takes IRSRMamba model as a baseline model, constructs a feature fusion module and a quantum superposition embedding module in the shallow feature extraction layer thereof, the feature fusion module comprising a shallow quantum feature refinement module and a wavelet transform; the Mamba deep feature extraction backbone is composed of a plurality of residual state space groups, each residual state space group comprising a plurality of quantum state space blocks; the quantum state space block comprises a two-dimensional selective scanning, a quantum inspiration state space module, a deep quantum feature refinement module and the like; and the image reconstruction layer comprises a quantum measurement reconstruction module. The present application effectively improves the super-resolution reconstruction effect of the slender plant microtubule image with topological continuity, and improves the accuracy and robustness of the filamentous feature extraction in the field of biological imaging and related industrial detection.

The application provides a low-temperature one-pot green synthesis method of CdSe quantum dots, and relates to the technical field of nanometer semiconductor materials, wherein cadmium oxide, selenium powder and long-chain fatty acid are mixed under an air atmosphere, a non-coordination solvent with a boiling point of greater than or equal to 250 DEG C is added, and stirring reaction is carried out at 160-250 DEG C for 5-180 minutes; and CdSe quantum dots are obtained after purification after cooling. The synthesized quantum dots have a narrow half-peak width (24 nm) and a large controllable range (485-650 nm), and have excellent performance, thereby providing strong support for the wide application of colloidal cadmium selenide quantum dots in the fields of optoelectronic devices, biological imaging and display technology; and the method is easy to scale up, can meet the needs of industrial large-scale preparation, and has good economic and social benefits.

The present invention relates to quantum dots comprising a core / shell structure. The quantum dots of the present disclosure form a compositional gradient between a core and a shell to enhance interfacial stability, to suppress defect-induced emission, and to provide high-efficiency and high-color purity emission characteristics through adjustment of an emission center wavelength and a reduction in an emission linewidth (FWHM). In addition, the present invention provides a high-performance light-emitting material that maintains optical stability of quantum dots for a long time through a protective effect of the outer shell and is suitable for various application fields such as displays, lighting, and bioimaging.

This invention relates to the field of biofilm microbial imaging and lineage analysis technology, and discloses a microbial analysis and processing method. The three-dimensional lineage tracking of individual microorganisms includes: identifying individual microorganisms in the biofilm through three-dimensional connectivity analysis; establishing individual movement trajectories across time points using a three-dimensional Kalman filter algorithm; detecting division events and establishing parent-child lineage associations based on trajectory changes, volume conservation, and spatial proximity; and finally assembling a four-dimensional spatiotemporal lineage diagram structure to represent the complete microbial proliferation and evolution process. This invention solves the technical problems of broken three-dimensional biofilm lineage tracking and loss of spatial diffusion trajectories in traditional two-dimensional analysis methods.

This invention provides a metal halide dual-mode luminescent material and its rapid microwave synthesis method, belonging to the field of luminescent material technology. Through precise ion doping design and controllable synthesis process, the metal halide dual-mode luminescent material of this invention achieves excellent luminescent performance, integrating up-conversion and down-conversion luminescence capabilities in the same system. It can be used in fields such as multi-mode optical anti-counterfeiting, information encryption, and integrated bio-imaging and diagnosis. The rapid microwave synthesis method is efficient and controllable, avoiding component volatilization and lattice defects caused by high-temperature and long-term processing. It achieves atomic-level uniform doping, has good process repeatability, is suitable for large-scale preparation, and meets the requirements of green synthesis and low-cost manufacturing.

The application relates to the technical field of photoelectric detectors, and specifically discloses a preparation method of a NiN type two-dimensional heterojunction infrared photoelectric detector. In view of the problem that the conventional architecture of an existing two-dimensional infrared detector is difficult to consider both dark current suppression and carrier transport efficiency, resulting in the fact that the detection sensitivity, response speed and photoelectric conversion efficiency of the device cannot be simultaneously improved, the core of the detector prepared by the application is an N-i-N heterostructure vertically stacked by ReS2 / 1T'-MoTe2 / WS2, and the preparation process comprises substrate pretreatment, two-dimensional material stripping and transferring and layer-by-layer stacking in an inert atmosphere, electrode patterning and evaporation, annealing treatment, and supporting in-situ phase state stabilization and interface passivation processes. By constructing a back-to-back double built-in electric field architecture, the application synchronously realizes dark current suppression and efficient separation of carriers, the prepared detector has excellent photoelectric performance, the process is simple and controllable, the compatibility with semiconductor processes is high, and the detector can be applied to the fields of intelligent sensing and biological imaging.

This invention discloses an organic semiconductor type I photosensitizer and a smart responsive organic semiconductor nanotherapy platform, belonging to the field of tumor bioimaging and treatment technology. The organic semiconductor type I photosensitizer constructed in this invention can achieve highly efficient and precise phototherapy through NIR-II fluorescence-specific imaging, activation of type I photodynamic therapy, and CO gas therapy. The smart responsive organic semiconductor nanotherapy platform based on this organic semiconductor type I photosensitizer has simple synthesis steps and simple separation and purification operations. It can synergistically enhance its PTT / PDT anti-tumor efficacy through CO-induced mitochondrial dysfunction, inhibition of heat shock protein expression, and induction of cell apoptosis. Therefore, it has good reference value and application potential in constructing anticancer drugs that achieve NIR-II FLI diagnosis and guide highly efficient photothermal and photodynamic therapy effects.