139 results about "Bio imaging" patented technology

The invention provides a nitrogen-doped biomass-based fluorescent carbon quantum dot, a preparation method and application, and relates to the technical field of carbon nanomaterials, the method comprises the following steps: mixing a biomass material and deionized water under an ultrasonic condition, adding a nitrogen source, and carrying out ultrasonic dispersion to obtain a uniformly dispersed to-be-reacted solution; reacting the to-be-reacted solution at a preset temperature for a preset time to obtain a mixed product solution; and filtering and dialyzing the mixed product solution to obtain the nitrogen-doped biomass-based fluorescent carbon quantum dot solution. The nitrogen-doped biomass-based fluorescent carbon quantum dot prepared by the method shows a stable fluorescent effect and good water solubility, the free radical clearance rate can reach 90% or above, and the antibacterial rate can reach 99% or above. The method can be used in the fields of drug sustained release, bioimaging and the like.

The invention discloses a beta-lactoglobulin fluorescent probe as well as a preparation method and application thereof, the structural formula of the probe is (C30H25N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, a fluorescence signal is triggered to be remarkably enhanced, and low-background fluorescence detection of the beta-lactoglobulin is realized. The probe has low cytotoxicity and excellent mitochondrial targeting ability, and provides a powerful tool for visualization of beta-lactoglobulin in living cells. After being combined with beta-lactoglobulin, a sample can generate obvious color change under the irradiation of an ultraviolet lamp, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, so that the rapid detection of the milk protein level in a beverage is realized. The beta-lactoglobulin fluorescent probe disclosed by the invention has a wide application prospect in the fields of biological imaging, milk protein detection, beverage quality control and the like.

The invention discloses a multi-photon optical fiber array endoscopic imaging device and method based on hollow spiral scanning, and belongs to the technical field of biological imaging. The device comprises a laser collimation and beam expansion module, a pulse chirp module, a light beam switching module, an endoscopic detection module and a fluorescence acquisition module. The light beam switching module comprises an optical switch and an optical fiber array composed of at least two low-group-velocity dispersion single-mode optical fibers. The endoscopic detection module drives the optical fiber array to execute hollow spiral scanning through the resonance driver, the track starting point is not located at the view field original point, and central dense sampling is avoided. According to the method, the optical fibers are switched through the optical switch, so that the optical fibers execute spatial non-coincident hollow spiral scanning, multi-photon signals such as fluorescence, second harmonic and fluorescence lifetime are acquired, a central hole is complemented through image reconstruction and fusion, and a complete field imaging result is obtained. Light damage of the central area is remarkably reduced, the imaging resolution uniformity is improved, the fused image is true and reliable based on physical sampling, and the system stability is high.

The invention belongs to the field of carbon nanomaterials, and provides a method for preparing a carbon quantum dot material doped with elements such as silicon and aluminum by oxidative depolymerization and stripping of coal gangue. Comprising the steps of screening and matching of coal gangue, oxidative depolymerization stripping, centrifugal dialysis membrane filtration and modification, drying and the like, raw materials and all the steps are specifically set, and the particle size or fluorescence performance of fluorescent carbon dots can be specifically selected and set according to selective oxidative depolymerization stripping conditions. The main particle size of the prepared multi-inorganic-element doped carbon quantum dot material is kept within the range of 1.5-2.0 nm, the structure is stable, the optical performance also meets the requirements, and the material can be further modified and is expected to be applied to the fields of photovoltaic equipment, biological imaging, photocatalytic degradation of organic pollutants or sensing and the like.

The invention discloses a bifunctional fluorescent molecular probe, a preparation method thereof and application of the bifunctional fluorescent molecular probe in polarity detection of ONOO <-> and lipid droplets, and belongs to the technical field of polarity detection of ONOO <-> and lipid droplets. The problems that a fluorescent probe is only limited to detection of a single analyte, and targeted monitoring of lipid droplets in cells and simultaneous detection of endogenous ONOO <-> in the cells cannot be achieved are solved. According to the invention, a dual-response fluorescent molecular probe PTQ based on a triphenylamine group is prepared, and the PTQ shows dual response to a polar environment and dynamic change of ONOO <->. Test results show that the fluorescent probe shows high sensitivity (1.92 nM), stable fluorescence signals and high quantum yield (57.76%) in a low-polarity medium, also has excellent ONOO <-> selectivity, low detection limit (4.97 nM), low toxicity and excellent biological imaging ability, and realizes targeted monitoring of lipid droplets and detection of endogenous ONOO <-> in cells.

The invention provides a green synthesis method and application of gold nanoclusters based on silk fibroin, and belongs to the technical field of metal nanoparticle treatment. Mixing the silk fibroin solution with a glutathione aqueous solution to obtain a GSH / SF mixed solution; and a HAuCl4 aqueous solution is added, so that the gold nanocluster is prepared by a one-pot method. The prepared gold nanocluster is excellent in performance in cell marking and zebra fish living body imaging, and the excellent characteristic shows that the SF-AuNCs has a wide prospect in biomedical imaging. Not only is the application potential of the silk fibroin in the field of biomedical imaging expanded, but also a high-performance protein fluorescent nanoprobe is provided for bioimaging.

The invention discloses a multi-cyano offset substituted pentamethine cyanine dye and a synthesis method and application thereof, and the dye has the following general formula structure: # imgabs0. According to the multi-cyano offset substituted pentamethine cyanine dye disclosed by the invention, the absorption emission wavelength is increased by introducing an offset substitution structure; the dye has near-infrared first-area imaging and treatment performance, so that the optimal excitation and emission wavelength can be flexibly selected, the dye shows obvious Stokes shift, reduction of self-absorption and scattering effects is facilitated, the imaging definition and depth are improved, the molar extinction coefficient of the prepared dye is up to 190000, and the dye can be applied to near-infrared first-area imaging and treatment of the near-infrared first-area imaging and treatment of the near-infrared first-area imaging and treatment of the near-infrared first-area imaging. According to the present invention, with the application of the dye, the light energy utilization degree of the dye is substantially improved, the long wavelength light with the deeper penetration depth can be strongly absorbed, the light energy capture and conversion can be effectively achieved, the photosensitizer efficiency can be improved, the light dose can be reduced, the phototoxicity can be reduced, the high absolute fluorescence quantum yield can be represented, and the biological imaging effect can be substantially improved; and the imaging quality is higher.

The invention discloses a fluorine-substituted pentamethine cyanine dye and a preparation method and application thereof, the fluorine-substituted pentamethine cyanine dye has a structure shown in a general formula I: # imgabs0 #, and by introducing fluorine atoms to an indole benzene ring and introducing different groups to an N site, accurate regulation and control of light stability of dye molecules are realized. The fluorine-substituted pentamethine cyanine dye prepared by the invention shows remarkable advantages in spectral characteristics, has a molar extinction coefficient higher than that of the traditional cyanine dye and has strong light energy absorption capacity, and is beneficial to improving the light energy utilization rate and reducing the dye dosage; the absolute fluorescence quantum yield reaches a high level, the intensity and sensitivity of imaging signals are remarkably enhanced, and a more sensitive and reliable tool is provided for biological imaging and molecular detection.

The present invention provides a large Stokes shift near-infrared fluorescent probe for detecting HClO, its preparation method and biological imaging application, belonging to the field of bioanalysis technology. Based on the strategy of modifying the substituents at the 3,6 positions of xanthene, the present invention constructs a large Stokes shift near-infrared fluorescent probe for specifically recognizing HClO. Optical experiments show that the emission wavelength of the probe for detecting HClO is 776 nm, the Stokes shift reaches 148 nm, with strong selectivity, high sensitivity, and a detection limit as low as 4.95 nM, which can greatly reduce the interference of background fluorescence in biological imaging applications. The fluorescent probe of the present invention has almost no cytotoxicity and can be applied to the detection and imaging of HClO in living cells MCF-7 and rat liver tissues, with a tissue penetration depth of 140 μm.

This invention provides a long-afterglow compound, system, preparation method, and application based on covalent bond coupling. The long-afterglow compound absorbs photons and transfers the absorbed photon energy within the molecule, undergoing a photochemical reaction that releases the energy as light, thus achieving afterglow luminescence. The structure of the long-afterglow compound is shown in Formula I, where X is selected from O or S; R is selected from a photosensitizer; and n is 1–15. The long-afterglow system includes a carrier medium and a long-afterglow compound, wherein the carrier medium is used to dissolve, disperse, or adsorb the long-afterglow compound. This invention can achieve near-infrared luminescence at 660 nm and 718 nm, and can be applied in fields such as bioimaging, immune detection, cell tracking, biosensing, and information encryption.

The invention provides a diatom-bridged fluorescent dye and application thereof, the diatom-bridged fluorescent dye is a compound represented by a formula A, R1 to R9 and Y <-> are defined in the description, the maximum ultraviolet absorption wavelength is about 620 nm, and the maximum fluorescence emission wavelength is about 650 nm. The diatom bridged fluorescent dye disclosed by the invention has excellent viscosity responsiveness, high fluorescence intensity and good stability, and has an extremely high application value in biological imaging. # imgabs0 #

The present invention discloses a method for generating sound waves in the air using a pulsed laser. The device for generating sound waves is composed of a pulsed laser and an optical modulation system. The pulsed laser is emitted by a pulsed laser and then acts on water vapor in the air after passing through the optical modulation system. The water vapor absorbs the heat energy of the pulsed laser. After the pulsed laser passes through, the water vapor temperature instantly rises and expands, thereby generating pressure waves in the area of ​​action, that is, generating sound waves through the photoacoustic effect. In addition, a three-color visible light laser is used to illuminate the area of ​​action for imaging. The structure of the method for generating sound waves in the air using laser pulses proposed by the present invention is simple. Since water vapor can still be generated even in air with low relative humidity, the effect can be achieved by only following the above steps. The method has research value in the manipulation of microparticles, biological imaging, space imaging, sound communication, and naked-eye three-dimensional display.

The present application relates to a kind of 2-phenyl substituted triarylethene photochromic material and preparation method and application, the prepared photochromic material can be by adjusting the steric hindrance and electron-withdrawing ability of 2-substituent group to the photochromic performance of triarylethene Realize significant regulation.This material has low raw material price, simple synthesis step, fast response and excellent fatigue resistance, so that the triarylethene material of original stimulus response single and between structure and photochromic property has no obvious rule is improved, is expected to have wide application prospect in high-speed information storage and fast biological imaging etc.In addition, the present application is suitable for anti-fake, photosensitive decoration, optical information storage, single-molecule logic gate, super-resolution fluorescence imaging, green printing and additive manufacturing etc. by different steric hindrance and electron-withdrawing substituent group to adjust photochromic color saturation.

The present invention relates to compounds of Formula I, in which Y, Ar1, Ar2, X, R1, and R2 are defined herein, and to their use in various biological imaging techniques and therapeutic methods. The present invention relates, in aspects, to conjugates comprising compounds of Formula I and their related uses and therapeutic uses.

The present invention relates to the technical field of silica glass optical fibers, and particularly to a neodymium-doped silica glass with a blue-shifted fluorescence spectrum to the 870 nm band and a preparation method thereof. The present invention proposes an innovative method to achieve the blue shift of Nd-doped silica glass and optical fibers from the conventional 900 nm to the 870 nm band. By co-doping with phosphorus elements, the local microstructure of Nd ions in the silica glass is precisely regulated, and its coordination environment is optimized, thereby enhancing the coordination field, promoting changes in the energy level distribution of Nd ions, successfully shifting the fluorescence peak of the neodymium-doped silica glass from 900 nm to 870 nm, effectively breaking through the limitations of applications in the 900 nm band, providing strong technical support for the applications of the 870 nm band in the fields of biological imaging, near-infrared light sources for quantum communication, and precision measurement, and further expanding the application prospects of Nd-doped silica glass in the fields of high-power lasers and advanced photonics.

The invention discloses a NaYF4 type luminescent material as well as a preparation method and application thereof. The preparation method comprises the following steps: reacting a raw material composition in a molten salt medium; wherein the raw material composition comprises a Y source, a Yb source, an Er source and a fluorinating agent, and the molten salt medium comprises nitrite, NaNO3 and KNO3. The preparation method is a one-step method, does not need an organic solvent or a surfactant, is simple and convenient to operate, is strong in red light up-conversion emission, and is suitable for large-scale production and application of a biological imaging system, sensing equipment, a lighting device or energy storage equipment and the like.

The application discloses a kind of multi-photon fiber array endoscopic imaging device and method based on hollow spiral scanning, belong to biological imaging technical field.The device includes laser collimation and beam expansion module, pulse chirp module, beam switching module, endoscopic detection module and fluorescence acquisition module.The beam switching module contains optical switch and at least two low group velocity dispersion single-mode fiber array;Endoscopic detection module drives fiber array to perform hollow spiral scanning by resonant driver, its track starting point is not located at the origin of field of view, to avoid central dense sampling.The method switches fiber by optical switch, so that each fiber performs spatial non-overlapping hollow spiral scanning, collects multi-photon signals such as fluorescence, second harmonic and fluorescence lifetime, and completes the central cavity by image reconstruction and fusion, to obtain complete field of view imaging result.The application significantly reduces the central region light damage, improves the uniformity of imaging resolution, and the fused image is based on physical sampling to ensure authenticity and reliability, with high system stability.

The present invention belongs to the field of medicinal chemistry technology and relates to a lysosomal-targeted nitroreductase-responsive fluorescent probe based on a triphenylamine backbone, its synthesis method, and its application. The triphenylamine derivative-based lysosomal-targeted nitroreductase-responsive fluorescent probe provided by the present invention does not emit light upon initial entry into cells. However, after a period of culture in cancer cells, the electron-withdrawing nitro group is reduced by the intracellular nitroreductase. Under laser irradiation, the charge within the fluorophore shifts, resulting in fluorescence. The present invention also provides a method for synthesizing the lysosomal-targeted nitroreductase-responsive fluorescent probe and its use in bioimaging.

The invention discloses a near-infrared fluorescent probe DCIC as well as a preparation method and biological imaging application thereof. According to the structure of the probe, (E)-2-(3-(3, 5-difluoro-4-hydroxystyryl)-5, 5-methylcyclohex-2-alkenyl) malononitrile is used as a near-infrared fluorophore, a dynamic response unit is constructed through acryloyl modification, and specific recognition and lasting signal output of Cys can be achieved. After the probe reacts with Cys, near-infrared fluorescence enhancement is generated at 650 nm, and the probe has the advantages of being short in response time, high in sensitivity and good in selectivity. Compared with the prior art, the rapid imaging of plant cells and living body Cys metabolism can be synchronously realized. The invention provides a high-sensitivity and high-temporal-spatial-resolution molecular tool for plant stress physiological research and disease-related Cys metabolism monitoring.

This invention discloses a method for preparing a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film, comprising: reacting a terminal amino-substituted alkane with halogen-modified naphthalenecarboxylic anhydride to synthesize an organic light-emitting small molecule with phosphorescent / fluorescent dual-emission function; and mixing the obtained organic light-emitting small molecule into a dynamically cross-linked supramolecular polymer to obtain a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film. This invention also discloses the single-component room-temperature phosphorescent / fluorescent dual-emission polymer film prepared by the above method and its applications. The preparation method of this invention is simple and low-cost. The polymer film prepared with only a single component can generate a phosphorescent / fluorescent dual-emission ratio light signal with stable emission. The film color changes with changes in ambient humidity, and the film can sense ambient humidity in the range of 1–100%, showing broad application prospects in sensing, anti-counterfeiting, and bioimaging fields.

This invention relates to a lysosome-targeting fluorescent dye based on the BODIPY structure and its synthesis method, belonging to the field of organic fluorescent dyes. The invention designs and synthesizes the lysosome-targeting fluorescent dye BDP. The dye molecule uses BODIPY as the parent structure, and a novel lysosome-targeting fluorescent dye molecule is synthesized by introducing diphenylphosphine salt and tert-butyloxycarbonyl groups. The synthetic route of this dye is simple, it possesses excellent photostability, and exhibits good resistance to interference from ions, amino acids, and viscosity. Furthermore, this dye demonstrates superior fluorescence performance in a variety of common solvents, with fluorescence quantum yields exceeding 0.70. Experimental results show that this fluorescent dye can precisely target lysosomes in living cells, demonstrating promising applications in the field of bioimaging.

The invention discloses an iridium complex as well as a preparation method and application thereof, and relates to the technical field of optogenetics. The iridium complex has the following structural formula, X is an anion, Y is selected from an alkane derivative, an ether derivative or an amine derivative, the iridium complex provided by the invention is regular in structure, the central metal atom iridium has excellent fluorescent property, and fluorine atoms and trifluoromethyl groups introduced into the ligand have excellent fluorescent property, so that the fluorescence intensity of the iridium complex is greatly improved. The electron structure and the energy level of the complex are obviously regulated, the photophysical performance of the complex is enhanced, and excellent two-photon absorption capacity is shown, so that the complex has huge application potential in the fields of biological imaging, photodynamic therapy and the like.

The invention discloses a probe group, a kit and a method for high-throughput in-situ detection of nucleic acid molecules in a biological sample, and belongs to the technical field of molecular biological imaging. A biological sample to be detected is contacted with a hairpin probe pair comprising a primary split probe pair, a secondary double-arm probe and different detectable marker modified hairpin probe pairs, the primary split probe pair anchors a target nucleic acid molecule, the secondary double-arm probe is connected to a split probe, and the hairpin probe pair is connected to the split probe pair. In addition, the initiation sequences and the hairpin probe carry out amplification polymerization on the initiation hybridization chain reaction, the analysis of the target nucleic acid molecule is realized by detecting the detectable marker, and as two different initiation sequences are simultaneously mounted on the secondary double-arm probe, the logic superposition of physical channels is realized, and the detection sensitivity is greatly improved. High-flux target detection is realized in a limited fluorescence channel, and meanwhile, the specificity and the signal-to-noise ratio are enhanced.

The invention discloses a fluorescent probe for high-sensitivity monitoring of cell mitochondrial autophagy as well as a preparation method and application thereof. The structure of the fluorescent probe is as shown in formula I in the specification. According to the fluorescent probe provided by the invention, a phenol group is used as an electron donor and a pH response unit, hydroxyl tricyanopyrrole is used as an electron acceptor, triphenylphosphine is used as a mitochondrial targeting group, fluorine atoms are introduced to a benzene ring to improve the response rate and the fluorescence intensity, and meanwhile, a thiophene ring is introduced to further expand the fluorescence emission wavelength. The probe can rapidly target mitochondria and emit corresponding fluorescence signals based on pH differences of different autophagy stages, so that real-time, high-sensitivity and high-selectivity monitoring of the mitochondria autophagy process is realized. In addition, the fluorescent probe is simple in molecular structure, small in molecular weight and easy to synthesize, purify and subsequently modify. Cell experiment results show that the probe can accurately trace the mitochondrial autophagy process in cells, and has good application potential in the field of biological imaging and detection.

The preparation method of the fluorescent probe (S) comprises the following steps: adding absolute ethyl alcohol into p-phenylenediamine, refluxing and dissolving the solution (solution A) while heating at 80 DEG C, dissolving salicylaldehyde into a proper amount of absolute ethyl alcohol (solution B), dropwise adding the solution B into the solution A, and stirring to obtain a mixed solution; heating reflux is kept at 80 DEG C in the whole process, stirring is continuously carried out, and after reflux is carried out for 2 hours, filtering is carried out to obtain a faint yellow solid, namely the fluorescent probe (S). The fluorescent probe can specifically recognize hydrogen sulfide (H2S) under the interference of common ions, is strong in anti-interference capability and high in sensitivity, and can be used for real-time monitoring and fluorescence imaging of endogenous H2S of cells and mouse tumors.

The application belongs to the field of polymer synthesis and material science, and specifically discloses a water-enhanced room-temperature phosphorescence-emitting polymer, a synthesis method and application thereof, wherein a linear polymer is synthesized from a polyisocyanate, an amide monomer and a silane coupling agent under the catalysis of self-catalysis or dibutyltin dilaurate, the polymer is coated on a base material, and can be cured and formed into an organic room-temperature phosphorescence coating at room temperature, and realizes room-temperature phosphorescence-enhanced emission after hydrolysis and curing. The required time for hydrolysis and curing can be effectively changed by adjusting the monomer ratio, and the method has the advantages of adjustable structure, low toxicity, wide monomer adaptability, simple operation, low monomer cost and the like. The prepared organic room-temperature phosphorescence polymer has excellent water-phase afterglow stability, and simultaneously has excellent adhesion and long-life afterglow, and can be applied to the fields of coating, biological imaging and anti-counterfeiting.

The invention discloses a polymer-based long-afterglow luminescent material as well as a preparation method and application thereof, and belongs to the field of organic luminescent materials. The polymer-based long-afterglow luminescent material is a host-guest doped material of an organic small molecule guest based on a benzo [b] phenothiazine derivative and a host of polymethyl methacrylate. The macroscopic afterglow time of the obtained long-afterglow luminescent material can reach the hour level. The obtained material has excellent machinability, transparency and flexibility, and can be used in the fields of environment-friendly illumination, advanced anti-counterfeiting, information encryption, 3D printing, biological imaging and the like.

This invention discloses a method for preparing a lysosome-targeted polar fluorescent probe and its application in bioimaging, belonging to the field of chemical analysis and detection technology. The structure of the polar fluorescent probe is as follows: The lysosome-targeted polar fluorescent probe of this invention exhibits excellent polar sensing properties and significant aggregation-induced emission characteristics. With increasing solvent polarity, the fluorescence emission of the probe PHPT shows a significant redshift and a marked increase in fluorescence intensity. Within the polarizability range of 0.214–0.353, the maximum emission wavelength of the probe PHPT shows a good linear relationship with the solvent polarizability. The probe also exhibits good photostability. More importantly, the polarity detection properties of the probe PHPT are unaffected by pH and various small biological molecules. The probe PHPT has low cytotoxicity and is suitable for bioimaging. PHPT has the ability to precisely target lysosomes and can be used to detect decreased cell polarity and increased polarity during autophagy, and can be used to monitor physiological activities related to changes in intracellular polarity.

The application discloses an InP / ZnS quantum dot-based TTA-UC luminescent material and a preparation method and application thereof. The InP / ZnS core-shell quantum dot is used as a triplet state sensitizing agent, and 1-pyrene carboxylic acid is used as a bridging molecule to form a quantum dot-PCA / / DPA ternary composite interface with an organic annihilator 9,10-diphenyl anthracene. Under the premise that the size of the quantum dot is not obviously changed, by adjusting the Br / Cl halogen ratio in the indium source and the zinc source, the quantum dot lattice defect passivation and the triplet state energy transfer level matching are simultaneously realized, so that the triplet state energy transfer efficiency of the system is significantly improved, and the highest (19.75%) up-conversion fluorescence quantum yield in the quantum dot composite system with DPA as the annihilator is obtained. The preparation scheme can be expanded to other TTA-UC systems with InP quantum dots as the sensitizing agent, and can be widely applied to the fields of photocatalysis, solar cells and biological imaging.

Disclosed is use of a second near-infrared (NIR-II) organic fluorescent compound in the preparation of a bioimaging contrast agent and in vascular fluorescence imaging. The NIR-II organic fluorescent compound having the structure represented by formula I could be used to construct a high-performance bioimaging contrast agent, and shows broad prospects in the field of in vivo imaging and fluorescent surgical navigation.