45 results about "Bio imaging" patented technology

This invention provides a long-afterglow compound, system, preparation method, and application based on covalent bond coupling. The long-afterglow compound absorbs photons and transfers the absorbed photon energy within the molecule, undergoing a photochemical reaction that releases the energy as light, thus achieving afterglow luminescence. The structure of the long-afterglow compound is shown in Formula I, where X is selected from O or S; R is selected from a photosensitizer; and n is 1–15. The long-afterglow system includes a carrier medium and a long-afterglow compound, wherein the carrier medium is used to dissolve, disperse, or adsorb the long-afterglow compound. This invention can achieve near-infrared luminescence at 660 nm and 718 nm, and can be applied in fields such as bioimaging, immune detection, cell tracking, biosensing, and information encryption.

The present invention relates to compounds of Formula I, in which Y, Ar1, Ar2, X, R1, and R2 are defined herein, and to their use in various biological imaging techniques and therapeutic methods. The present invention relates, in aspects, to conjugates comprising compounds of Formula I and their related uses and therapeutic uses.

The application discloses a kind of multi-photon fiber array endoscopic imaging device and method based on hollow spiral scanning, belong to biological imaging technical field.The device includes laser collimation and beam expansion module, pulse chirp module, beam switching module, endoscopic detection module and fluorescence acquisition module.The beam switching module contains optical switch and at least two low group velocity dispersion single-mode fiber array;Endoscopic detection module drives fiber array to perform hollow spiral scanning by resonant driver, its track starting point is not located at the origin of field of view, to avoid central dense sampling.The method switches fiber by optical switch, so that each fiber performs spatial non-overlapping hollow spiral scanning, collects multi-photon signals such as fluorescence, second harmonic and fluorescence lifetime, and completes the central cavity by image reconstruction and fusion, to obtain complete field of view imaging result.The application significantly reduces the central region light damage, improves the uniformity of imaging resolution, and the fused image is based on physical sampling to ensure authenticity and reliability, with high system stability.

This invention discloses a method for preparing a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film, comprising: reacting a terminal amino-substituted alkane with halogen-modified naphthalenecarboxylic anhydride to synthesize an organic light-emitting small molecule with phosphorescent / fluorescent dual-emission function; and mixing the obtained organic light-emitting small molecule into a dynamically cross-linked supramolecular polymer to obtain a single-component room-temperature phosphorescent / fluorescent dual-emission polymer film. This invention also discloses the single-component room-temperature phosphorescent / fluorescent dual-emission polymer film prepared by the above method and its applications. The preparation method of this invention is simple and low-cost. The polymer film prepared with only a single component can generate a phosphorescent / fluorescent dual-emission ratio light signal with stable emission. The film color changes with changes in ambient humidity, and the film can sense ambient humidity in the range of 1–100%, showing broad application prospects in sensing, anti-counterfeiting, and bioimaging fields.

This invention relates to a lysosome-targeting fluorescent dye based on the BODIPY structure and its synthesis method, belonging to the field of organic fluorescent dyes. The invention designs and synthesizes the lysosome-targeting fluorescent dye BDP. The dye molecule uses BODIPY as the parent structure, and a novel lysosome-targeting fluorescent dye molecule is synthesized by introducing diphenylphosphine salt and tert-butyloxycarbonyl groups. The synthetic route of this dye is simple, it possesses excellent photostability, and exhibits good resistance to interference from ions, amino acids, and viscosity. Furthermore, this dye demonstrates superior fluorescence performance in a variety of common solvents, with fluorescence quantum yields exceeding 0.70. Experimental results show that this fluorescent dye can precisely target lysosomes in living cells, demonstrating promising applications in the field of bioimaging.

The invention discloses a probe group, a kit and a method for high-throughput in-situ detection of nucleic acid molecules in a biological sample, and belongs to the technical field of molecular biological imaging. A biological sample to be detected is contacted with a hairpin probe pair comprising a primary split probe pair, a secondary double-arm probe and different detectable marker modified hairpin probe pairs, the primary split probe pair anchors a target nucleic acid molecule, the secondary double-arm probe is connected to a split probe, and the hairpin probe pair is connected to the split probe pair. In addition, the initiation sequences and the hairpin probe carry out amplification polymerization on the initiation hybridization chain reaction, the analysis of the target nucleic acid molecule is realized by detecting the detectable marker, and as two different initiation sequences are simultaneously mounted on the secondary double-arm probe, the logic superposition of physical channels is realized, and the detection sensitivity is greatly improved. High-flux target detection is realized in a limited fluorescence channel, and meanwhile, the specificity and the signal-to-noise ratio are enhanced.

The invention discloses a fluorescent probe for high-sensitivity monitoring of cell mitochondrial autophagy as well as a preparation method and application thereof. The structure of the fluorescent probe is as shown in formula I in the specification. According to the fluorescent probe provided by the invention, a phenol group is used as an electron donor and a pH response unit, hydroxyl tricyanopyrrole is used as an electron acceptor, triphenylphosphine is used as a mitochondrial targeting group, fluorine atoms are introduced to a benzene ring to improve the response rate and the fluorescence intensity, and meanwhile, a thiophene ring is introduced to further expand the fluorescence emission wavelength. The probe can rapidly target mitochondria and emit corresponding fluorescence signals based on pH differences of different autophagy stages, so that real-time, high-sensitivity and high-selectivity monitoring of the mitochondria autophagy process is realized. In addition, the fluorescent probe is simple in molecular structure, small in molecular weight and easy to synthesize, purify and subsequently modify. Cell experiment results show that the probe can accurately trace the mitochondrial autophagy process in cells, and has good application potential in the field of biological imaging and detection.

The preparation method of the fluorescent probe (S) comprises the following steps: adding absolute ethyl alcohol into p-phenylenediamine, refluxing and dissolving the solution (solution A) while heating at 80 DEG C, dissolving salicylaldehyde into a proper amount of absolute ethyl alcohol (solution B), dropwise adding the solution B into the solution A, and stirring to obtain a mixed solution; heating reflux is kept at 80 DEG C in the whole process, stirring is continuously carried out, and after reflux is carried out for 2 hours, filtering is carried out to obtain a faint yellow solid, namely the fluorescent probe (S). The fluorescent probe can specifically recognize hydrogen sulfide (H2S) under the interference of common ions, is strong in anti-interference capability and high in sensitivity, and can be used for real-time monitoring and fluorescence imaging of endogenous H2S of cells and mouse tumors.

The invention discloses a polymer-based long-afterglow luminescent material as well as a preparation method and application thereof, and belongs to the field of organic luminescent materials. The polymer-based long-afterglow luminescent material is a host-guest doped material of an organic small molecule guest based on a benzo [b] phenothiazine derivative and a host of polymethyl methacrylate. The macroscopic afterglow time of the obtained long-afterglow luminescent material can reach the hour level. The obtained material has excellent machinability, transparency and flexibility, and can be used in the fields of environment-friendly illumination, advanced anti-counterfeiting, information encryption, 3D printing, biological imaging and the like.

The invention discloses a near-infrared light-emitting AgAuSe quantum dot and a room-temperature macro preparation method thereof, and belongs to the technical field of inorganic semiconductor material preparation. The method comprises the following steps: dissolving a silver source in a mixed solution of aliphatic amine and an organic solvent to obtain a silver precursor; dissolving a selenium source in a mixed solution of aliphatic amine and alkyl mercaptan to obtain a selenium precursor; a gold source is dispersed in an organic solvent to obtain a gold precursor. And mixing the three components, stirring for reaction, and centrifuging. The method is easy and convenient to operate, high temperature and inert gas protection are not needed, toxic phosphine-containing reagents are not used, the near-infrared light-emitting AgAuSe quantum dots with excellent fluorescence performance, high photo-thermal stability and good biocompatibility can be rapidly synthesized at the room temperature in a macro-quantity mode through a one-step method, the absolute fluorescence quantum yield can exceed 90%, and the near-infrared light-emitting AgAuSe quantum dots can be widely used. Wide application prospects are realized in the fields of biological imaging and near-infrared photoelectric devices.

The invention provides a 7-ethyl-10-hydroxycamptothecin drug precursor with fluorescence activity and a preparation method and use thereof. The method involves that a carboxyl group of a boron dipyrromethene dye molecule is condensed with a hydroxyl group at the 10 position of 7-ethyl-10-hydroxycamptothecin, yielding a 7-ethyl-10-hydroxy-camptothecin drug precursor (BDP-SN38) with fluorescent activity. BDP-SN38 shows good solubility and stability in most pharmaceutically acceptable solvents. Compared with SN38, BDP-SN38 exhibits comparable antitumor activity, has high tumor uptake capacity, and produces strong green fluorescence with the excitation of visible light, thus providing fluorescence-guided bioimaging and further treatment of residual tumors after drug treatment, and having good application prospects in the research of integrated diagnosis and treatment and effective tumor treatment.

The application discloses a preparation method of a gallium germanate near-infrared luminescent material, and the luminescent material has a chemical formula of Ga 3+x‑y Al 3‑ x Ge2O 13 : y Cr 3+ According to a stoichiometric ratio of each raw material in the chemical formula, each raw material is weighed, Ga is introduced through an oxide, Al is introduced through an oxide or a hydroxide, Ge is introduced through an oxide, and Cr is introduced through an oxide or a nitrate; the raw materials are fully mixed and ground to obtain a raw material powder; the raw material powder is kept at a certain temperature in an air atmosphere, is cooled to room temperature in a furnace, is ground, and the near-infrared luminescent material is prepared. The preparation method can obtain the near-infrared luminescent material which has the advantages of a spectrum peak greater than 800 nm, adjustable spectrum, high luminescent efficiency (an external quantum efficiency is greater than 30%), good thermal stability (a residual luminescent intensity at 150 o C is greater than 60% at room temperature), can be efficiently excited by a blue light chip, and the like, can be applied to biological imaging, plant illumination, night monitoring and food detection, the preparation method is simple, no waste water and waste gas are discharged, and the preparation method is suitable for large-scale industrial production.

This invention relates to a low-dose aggregation-induced emission dye (AIE) bioimaging method, comprising the following steps: selecting a test organism and injecting it with a low dose of AIE; stimulating the test organism with an adjustable frequency excitation light source; acquiring fluorescence signals emitted from the test organism at equal time intervals; processing the fluorescence signals and generating a fluorescence image; applying phase-locked processing to the fluorescence image to separate the fluorescence signal component with the same frequency as the excitation light source and generating a phase-locked imaging result. By convolving the amplitude signal of each pixel in the fluorescence image with a sine or cosine signal with the same frequency as the excitation source, the orthogonality of trigonometric functions is utilized to effectively suppress noise, thus preserving the fluorescence signal that changes with the same frequency as the periodic light source. This significantly improves the signal-to-noise ratio of the fluorescence image, enabling clear observation of the AIE fluorescence signal even at low doses.

The invention discloses a near-infrared luminescent glass ceramic material and a preparation method thereof. The crystal phase of the glass ceramic material is Li2Mg2A4O11: xCr < 3 + >, yNi < 2 + >, and the element A is selected from one or more of Si, Ge, Ti and Zr; x is more than or equal to 0% and less than or equal to 15%, y is more than or equal to 0% and less than or equal to 15%, and x and y are not 0 at the same time. The glass ceramic is prepared by high-temperature melting and amorphous crystallization treatment. The obtained near-infrared luminescent glass ceramic can be efficiently excited by blue light and red light, the emission peak wavelength covers 650-1500nm, and the prepared near-infrared LED light source can be applied to the fields of food detection, security and protection, biological imaging, illumination and sensing.

The present application belongs to the technical field of image processing, and particularly relates to a plant microtubule image super-resolution reconstruction method and system based on quantum inspiration. The method of the present application takes IRSRMamba model as a baseline model, constructs a feature fusion module and a quantum superposition embedding module in the shallow feature extraction layer thereof, the feature fusion module comprising a shallow quantum feature refinement module and a wavelet transform; the Mamba deep feature extraction backbone is composed of a plurality of residual state space groups, each residual state space group comprising a plurality of quantum state space blocks; the quantum state space block comprises a two-dimensional selective scanning, a quantum inspiration state space module, a deep quantum feature refinement module and the like; and the image reconstruction layer comprises a quantum measurement reconstruction module. The present application effectively improves the super-resolution reconstruction effect of the slender plant microtubule image with topological continuity, and improves the accuracy and robustness of the filamentous feature extraction in the field of biological imaging and related industrial detection.

The application provides a near-infrared two-region fluorescent dye with mitochondrion targeting and aggregation-induced emission properties and application thereof in biological fluorescence imaging and tumor treatment. 41 H 37 F6N2PS, which has good light stability, outstanding mitochondrion targeting, clear ROS production mechanism and excellent photothermal performance. The substance has good mitochondrion targeting capacity and can destroy tumor mitochondria under laser irradiation of a certain intensity, thereby killing tumor cells. The aggregation-induced emission property makes it different from traditional aggregation quenching emission materials and has better biological imaging effect. The near-infrared property makes it have good tissue penetration capacity and biological fluorescence imaging effect. The substance has wide application prospects in near-infrared fluorescence imaging, preparation of mitochondrion targeting reagents and photodynamic therapy reagents. It can be applied to combined phototherapy of breast tumor PDT and PTT, and has simple synthesis steps and is conducive to commercial application and popularization.

The invention discloses a nitrogen-doped carbon dot, a preparation method thereof and application of the nitrogen-doped carbon dot in pH detection and cell imaging, and provides an efficient strategy for preparing nitrogen-doped, in-situ passivated and amino-functionalized nitrogen-doped carbon dots through a one-step hydrothermal method based on the structural characteristics of a precursor. The prepared nitrogen-doped carbon dots have the excellent properties of high quantum yield, independent excitation, good stability, good biocompatibility and the like. Besides, rich amino groups introduced by diethylenetriamine enable the quantum dot to have good pH response property, fluorescence-colorimetric dual-mode detection within the pH range of 2.0-7.0 can be realized, and high-intensity emission can be maintained in strong base (pH is 14.0). Meanwhile, by means of halogen removal, the heavy atom effect is inhibited, the quantum yield is increased, the biotoxicity (dark toxicity and phototoxicity) of the compound is remarkably reduced, and the application of the compound in the field of biological imaging is expanded.

The invention discloses a fluorescent carbon nanocluster taking polyethylene glycol as a template and having blue fluorescence emission capability as well as a synthesis method and application of the fluorescent carbon nanocluster, and belongs to the technical field of chemical synthesis and biological analysis and detection. The fluorescent carbon nanocluster is obtained by stacking dozens of xylose molecules on a polyethylene glycol template molecule through a simple hydrothermal synthesis method, has good water solubility and chemical stability, and has a molecular structure and fluorescence emission capability similar to those of a metal fluorescent nanocluster; the problem of heavy metal leakage of a traditional metal fluorescent nano-cluster probe is solved, so that the metal fluorescent nano-cluster probe has higher safety and biocompatibility, and has a wide application prospect in the fields of fluorescence sensing, biological imaging, environmental analysis and the like. Based on the fluorescence enhancement effect of an antibiotic drug oxytetracycline on the fluorescent carbon nano-cluster probe, high-sensitivity analysis and detection on the oxytetracycline can be realized.

This invention discloses a sample introduction probe device and method based on a membrane-assisted strategy. The device employs a membrane-assisted strategy to achieve in-situ pretreatment, injection, transfer, separation, and detection of trace amounts of complex samples under pressure. The main advantages of this invention include: integrating trace sample pretreatment with injection, chromatographic separation, and detection systems; a simple system structure; rapid and automated operation; high flexibility; ease of use; and wide applicability, particularly suitable for the analysis of trace samples. The membrane-assisted strategy enables in-situ sample pretreatment followed by injection, providing a new injection mode for the analysis of complex samples and offering a new approach to the integration and miniaturization of chromatographic analysis systems. This invention has broad application prospects in fields such as bioanalysis, organic synthesis, bioengineering, clinical mass spectrometry, single-cell analysis, analysis of trace complex samples, multi-omics analysis, and bioimaging analysis.

The invention provides a molten salt preparation method of a rare earth ion doped fluoride scintillating material, aims to provide a high-performance long-afterglow scintillating material which is high in luminous efficiency, good in thermal stability and suitable for novel anti-counterfeiting and biological imaging, and relates to the field of luminescent materials. The material is synthesized by a molten salt method, and has the advantages of good repeatability and easiness in large-scale production. By utilizing the efficient luminescence characteristic of rare earth ions in a calcium fluoride matrix, intense light emission can be generated under X-ray excitation, and the luminescence intensity and the doping concentration are adjustable; the luminescent material has a stable crystal structure, can still keep excellent luminescent property in a high-temperature environment, is suitable for manufacturing anti-counterfeiting marks, safety ink and anti-counterfeiting labels, and has a good application prospect in the fields of information encryption, certificate counterfeiting prevention, high-value product authentication and the like.

The invention relates to the technical field of material synthesis and biological imaging analysis, in particular to a lipid droplet targeting fluorescent probe, a preparation method and application thereof and a method for quantitatively detecting diglyceride in lipid droplets. The method for quantitatively detecting diglyceride in lipid droplets comprises the following steps: taking fluorescein hydrazide and coumarin as raw materials, carrying out hydrothermal reaction to obtain supernate, and sequentially carrying out column chromatography purification, rotary evaporation and drying on the supernate to obtain the lipid droplet targeted fluorescent probe. And constructing a linear equation between the fluorescence lifetime of the lipid droplet targeting fluorescent probe and the concentration of diglyceride in the lipid droplet. The lipid droplet targeting fluorescent probe is placed in a lipid droplet of which the content of diglyceride is to be detected, the fluorescence lifetime of the lipid droplet targeting fluorescent probe is obtained, and the content of diglyceride in the lipid droplet is obtained through a constructed linear equation. According to the scheme, the content of diglyceride in the lipid droplet is quantitatively detected, and the method has important research value and application prospect for promoting lipid droplet function research and mechanism analysis of lipid droplet metabolism related diseases.

The application belongs to the field of medical material application, and particularly relates to application of lanthanum-doped zinc oxide quantum dots as a carrier in preparation of an antitumor active substance. An ethanol solution of zinc acetate containing lanthanum and an ethanol solution of a strong base are mixed and reacted in an ice bath to obtain an ethanol solution of lanthanum-doped ZnO quantum dots, then n-hexane is added for extraction, centrifugal separation is performed, supernatant is removed, and vacuum drying is performed to obtain lanthanum-doped ZnO quantum dots; the lanthanum-doped ZnO quantum dots are modified by polyethylene glycol and hyaluronic acid to obtain a HA-PEG-La-ZnO composite nano drug carrier platform. The method is simple, low in cost, and beneficial to large-scale production; the lanthanum-doped ZnO quantum dots have high fluorescence intensity and fluorescence quantum yield, and can be used in the fields of biological imaging, drug carriers and the like.

The invention relates to the technical field of biological medicine, in particular to a pyrenyl colorful photosensitizer and a preparation method and application thereof, and the chemical structure of the pyrenyl colorful photosensitizer is shown in the specification. The pyrenyl colorful photosensitizer constructed by the invention can realize blue-green-yellow-orange-red multicolor adjustable fluorescence emission, has the advantages of ACQ and AIE, overcomes the defects of ACQ and AIE, is applied to the aspects of antibiosis, anti-tumor, biological imaging and the like, and realizes dual application of imaging and treatment. The implementation of the invention is expected to develop a new antibacterial and antitumor drug, and provides a new thought for developing novel photosensitizers and antibacterial and antitumor drugs.

The invention discloses an N-arylindole AIE molecule as well as a preparation method and application thereof, and belongs to the field of organic small molecule photo-thermal materials. The problems that an existing organic fluorescent material is long in synthetic route, part of fluorescent molecules contain pyridine structural units, DNA information extraction in fingerprint remaining biological residues is interfered, fluorescence in an aggregation state is weak, DNA information extraction in fingerprint remaining biological residues is interfered, the biological residues easily cause chemical damage, feature integrity is affected, and the like are solved. And during imaging, background interference is easily caused, and high-contrast and high-resolution visualization of three-level features of the fingerprints is difficult to realize. The chemical general formula of the N-arylindole AIE molecule is shown in the specification. The preparation method comprises the following steps: mixing indole, a catalyst and an anhydrous organic solvent, then adding halogenated aromatic hydrocarbon or halogenated aryl nitrile, and continuously stirring; the invention is applied to latent fingerprint development, biological imaging, fluorescent probes or sensors.

An image processing device includes circuitry configured to receive a first visible and an invisible image, remove the invisible component from the first visible image based on the invisible image to generate a second visible image, determine whether to remove the invisible component from the first visible image, and output either the first visible image based on a determination not to remove the invisible component or output the second visible image based on a determination to remove the invisible component. The first visible image is captured by a first sensor having a first sensitivity to both of first light in a visible wavelength range; and second light in an invisible wavelength range. The first visible image includes an invisible component in the invisible wavelength range. The invisible image is captured by a second sensor having a second sensitivity to the second light.

The invention relates to the technical field of medicines, in particular to puerarin carbon dots as well as a synthesis method and application thereof. The puerarin carbon dots are synthesized by taking puerarin as a carbon source and m-phenylenediamine as a nitrogen source. The invention also provides a synthetic method of puerarin carbon dots, which comprises the following steps: dispersing puerarin in an inorganic solvent to obtain a solution A; dissolving m-phenylenediamine in an inorganic solvent to obtain a solution B; adding the solution B into the solution A, heating, reacting, cooling and filtering to obtain filtrate; and filtering the filtrate by using a filter membrane, dialyzing the filtrate, and freeze-drying to obtain the puerarin carbon dots. The invention further provides application of the puerarin carbon dots in preparation of a disease diagnosis and treatment integrated reagent. The invention solves the problems that the existing puerarin is poor in water solubility, low in bioavailability, weak in self-fluorescence and difficult to be used for biological imaging, and the existing puerarin is difficult to be directly used for a nano diagnosis and treatment system.

CHITOSAN-MODIFIED DURIAN SEED GUM AS A CANCER CELL BIOIMAGING AGENT This invention relates to a natural polymer material product in the form of polyelectrolyte complex particles composed of durian seed gum and chitosan. The product is made by mixing a solution of durian seed gum and a solution of chitosan to form colloidally stable submicron-sized particles. The particles are responsive to pH changes and exhibit improved kinetic stability and surface characteristics compared to unmodified durian seed gum. The resulting material exhibits intrinsic fluorescence without the addition of external fluorescent agents and has selective activity against cancer cells with high biocompatibility towards normal cells. This invention can be used as a bioimaging material, an intracellular tracking system, a carrier for active compounds, and a supporting material for natural-based cancer therapy.

The application relates to a preparation method of circularly polarized luminescent thermally activated delayed fluorescence material, and the circularly polarized luminescent thermally activated delayed fluorescence material is non-toxic and harmless, can realize the effect of thermally activated delayed fluorescence through self-assembly by aggregation in a mixed solution of an aqueous phase and an organic phase, and can be used for devices and cell imaging. After phagocytosis of HeLa cells, cell imaging can be realized through time-resolved means, so that a more complete effect of imaging is realized, and a clearer imaging effect is ensured; the thermally activated delayed fluorescence material can realize the effect of fluorescence-phosphorescence conversion through oxygen removal in a solution. The material structure is controllable, the property is stable, the performance of phosphorescent material and thermally activated delayed fluorescence is combined, more complete and clear time-resolved imaging is realized, and a research foundation is provided for in-depth research of biological imaging.

The invention relates to the field of organoboron chemistry and material chemistry. The invention discloses a boron-based framework compound as well as a preparation method and application thereof. The compound is characterized by having a specific skeleton structure formed by coordination of an N, O-bidentate ligand and a boron center. The preparation method adopts a one-pot method or a fractional step method, the key point is that hexafluoroisopropanol is used as a single reaction solvent, or a mixed solvent system of perfluorinated pinacoline alcohol and chloroform is used, and the system obviously improves the chemical stability of the product. The method is simple in synthesis process, high in yield and easy to purify. The obtained series of compounds have good solubility in organic solvents and show excellent fluorescence properties, the highest fluorescence quantum yield can reach 75.48%, and the emission wavelength is adjustable. The compounds have wide application prospects in the fields of photoelectric materials such as organic light emitting diodes, fluorescence sensing, biological imaging and solar cells.

The invention discloses a disulfide bond bridging reagent based on a sulfuryl pyridine derivative, a conjugate as well as a preparation method and application of the disulfide bond bridging reagent. The disulfide bond bridging reagent provided by the invention can be used for re-bridging disulfide bonds in biomolecules (polypeptide and protein) containing disulfide bonds and further coupling small molecules and functional molecules. The invention also provides a preparation method and application of the conjugates, including but not limited to bioimaging and targeted therapy. The bridging reagent is good in reactivity, the generated conjugate is high in stability, successful coupling of small molecules shows that the coupling method has the potential of coupling various functional molecules, and coupling and application of part of functional groups are carried out. And a novel tool integrating water solubility, reactivity, product stability and functional group coupling is provided for biomolecule coupling.