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Method for the production of bio-imaging nanoparticles with high yield by early introduction of irregular structure

A nanoparticle and biological imaging technology, applied in nanostructure manufacturing, nanotechnology for materials and surface science, nanomedicine, etc., can solve the problem of low reaction yield

Inactive Publication Date: 2009-05-06
KOREA INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disclosed method still has the problem of low reaction yield because the hydrophilic thiol groups linked by long hydrocarbon chains must penetrate into the surface of the nanoparticles surrounded by surfactants.

Method used

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  • Method for the production of bio-imaging nanoparticles with high yield by early introduction of irregular structure
  • Method for the production of bio-imaging nanoparticles with high yield by early introduction of irregular structure
  • Method for the production of bio-imaging nanoparticles with high yield by early introduction of irregular structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Preparation of semiconductor nanoparticles CdSe / CdS-DA by surface ligand exchange

[0065] Vacuum evaporation of 5ml CdSe / CdS-ODA hydrophobic quantum dot solution (8 × 10 -5 M) to remove the solvent and disperse in 20ml of chloroform. To this dispersion was added 1000 equivalents of decylamine (DA) and the mixture was stirred for 2 days in a dark inert atmosphere. The resulting solution was mixed with acetone and centrifuged to separate the precipitate. The thus prepared precipitate was dispersed in chloroform to prepare 20ml of CdSe / CdS-DA solution (2×10 -5 M). The CdSe / CdS-DA samples were analyzed by infrared spectrometer and transmission electron microscope (TEM), where the image 3 (b) and Figure 4 The results are shown in (b). The exchange of surface ligands was confirmed by the shorter distance between CdSe / CdS-DA quantum dots than CdSe / CdS-ODA in the TEM images.

Embodiment 2

[0066] Example 2: Preparation of partially surface-modified semiconductor nanoparticles CdSe / CdS(-DA)ex(-MUA)

[0067]Add 5 equivalents of mercaptoundecanoic acid (MUA) to 17 ml of the CdSe / CdS-DA solution prepared in Example 1, and stir for 19 hours in a dark inert atmosphere. The resulting solution was concentrated, mixed with acetone, and the precipitate was separated by centrifugation. The thus prepared precipitate was dispersed in chloroform to prepare 17ml of CdSe / CdS(-DA)ex(-MUA)5 solution (2×10 -5 M). The obtained samples were analyzed by infrared spectrometer and transmission electron microscope (TEM), in which image 3 (c) and Figure 4 The results are shown in (c). The TEM images confirmed that the self-assembly of the nanoparticles no longer occurred, which was attributed to the disruption of their homogeneous structure caused by the partial replacement of MUA, as figure 2 as shown in step C.

Embodiment 3

[0068] Example 3: Preparation of targeted hydrophobic semiconductor nanoparticles CdSe / CdS(-DA)ex(-MUA-en-FA)5

[0069] 2 ml of the CdSe / CdS(-DA)ex(-MUA)5 solution prepared in Example 2 was diluted with chloroform to a final volume of 10 ml. After adding 5 equivalents of dicyclohexylcarbodiimide (DCC) to the diluent and stirring for 3 hours in a dark inert atmosphere, 50 equivalents of en-FA prepared as described below was added thereto and stirred for a further 2 hours . The resulting solution was concentrated, mixed with acetone, and the precipitate was separated by centrifugation. The thus prepared precipitate was dispersed in chloroform to prepare 10 ml of CdSe / CdS(-DA)ex(-MUA-en-FA)5 solution (4×10 -6 M). The binding of en-FA to MUA was analyzed by infrared spectrometer and TEM, where respectively in image 3 (d) and Figure 4 The results are shown in (d).

[0070] Preparation of complex en-FA targeting molecule folic acid (FA) and ethylenediamine (en)

[0071] 441...

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Abstract

The present invention discloses bio-imaging nanoparticles through introducing irregular structure early and preparation method thereof. The aim of the invention is to provide a method of preparing bio-imaging nanoparticles, wherein by means of part surface modification, introducing irregular structure into the surface of the nanoparticles, and allowing aggregation and deposition phenomenas in the structure caused by the hydrogen bond attraction inside the nanoparticles, the bio-imaging nanoparticles having high dispersibility in an aqueous solution, biocompatibility, and targetability with high yield, are prepared without loss of nanoparticles.

Description

technical field [0001] The present invention relates to a method for preparing bioimaging nanoparticles with high dispersibility, biocompatibility and targeting in aqueous solution with high yield by early introduction of irregular surface structure. Background technique [0002] Since a method of chemically synthesizing hydrophobic inorganic nanoparticles having a uniform size distribution in a surfactant-containing organic solvent has been established, various attempts have been made to put this method to use. In particular, since nanoparticles prepared in aqueous solutions exhibit much larger non-uniform size distributions than those prepared in organic solvents and in water, the cheapest, most environmentally friendly, and most useful solvents existing on Earth, the Surface modification of hydrophobic nanoparticles with uniform size distribution prepared in organic solvents for stable dispersion in aqueous solutions is of great importance and is a major area of ​​focus f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18C01G11/02
CPCA61K49/1839A61K49/0052A61K49/0067B82Y5/00A61K49/1836A61K49/186B82B3/00B82Y30/00
Inventor 禹庚子文智炯
Owner KOREA INST OF SCI & TECH
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