173 results about "Arbitrary Fluorescence Unit" patented technology

A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health. The system includes an enclosure for placement around a plant to be imaged in-situ. There is a controlled light source that controllably provides to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure. The chlorophyll fluorescence emitted from the plant both spatially and temporally is captured, and the captured fluorescence information is analyzed to provide plant health information.

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

The invention discloses a high-throughput plant phenotype analysis device based on an optical imaging technique.The device comprises a light shading cover, a base, a top frame, a material carrying table, a sensor panel, a chlorophyll fluorescence imager, a near-infrared multi-spectral facial form imager, an infrared thermal imager, a three-dimensional reconstruction imager and a light source set; the base is installed in the light shading cover, and a working position is arranged in the middle of the base; the top frame is installed in the light shading cover and fixed over the base; the material carrying table is installed on the working position of the base; the sensor panel is installed on the top frame through a translation mechanism; the chlorophyll fluorescence imager, the near-infrared multi-spectral facial form imager, the infrared thermal imager and the three-dimensional reconstruction imager are installed on the sensor panel; the light source set is installed on the sensor panel and supplies exciting light and illuminating light to the imagers.The invention further discloses a high-throughput plant phenotype analysis method based on the optical imaging technique.According to the device and method, use is convenient, complete plant phenotype data can be acquired, and the test result is accurate.

The invention provides a method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement, which comprises the following steps of: obtaining plant leaf blades, shading environment light, setting multiple light intensity gradients, irradiating for a preset time with each light intensity gradient and measuring practical photosynthetic efficiency and relative electron transmission rate; drawing a response curve of the relative electron transmission rate, which is changed along the light intensity and fitting the response curve to obtain semi-saturated light intensity; taking the semi-saturated light intensity as the actinic light intensity for measuring the chlorophyll fluorescence induction curve; calculating a difference value between a maximal fluorescence value after light adaptation and a practical fluorescence value and a difference value between a maximal fluorescence value after dark adaptation and a basic fluorescence value after dark adaptation, and calculating the ratio of the two difference values; judging if the ratio is in a preset range, if so, confirming the current light intensity as the actinic light intensity, and if the ratio is less/more than the lower limit/the upper limit of the preset range, reducing/increasing the light intensity, re-measuring the chlorophyll fluorescence induction curve and calculating the ratio. The method can measure typical chlorophyll fluorescence induction curve.

The invention discloses a method for rapidly and quantitatively calculating inherent drought resistance of plants. The method comprises the following steps: cleaning to-be-measured plant leaves, soaking the plant leaves in water, taking out the leaves after 30 minutes, and absorbing water on the surface; measuring minimal fluorescence (Fo) and PSII maximum photochemical quantum yield (Fv/Fm) of the leaves during 0 level fluorescence by using an IMAGING-PAM modulation type chlorophyll fluorescence spectrometer, and repeating for three times; then allowing the leaves to lose water, and repeating the operation every an hour; taking the measurement result during waterlogged 0 hour as reference, calculating relative Fo and relative Fv/Fm at each measurement moment, and respectively adding to obtain the accumulated relative Fo (TRSF) and accumulated relative Fv/Fm (TRPF) in the previous five hours after waterlogging; and comparing the numerical values of the TRSF and TRPF, and quantifying the inherent drought resistance of different plants. The method has the advantages of high accuracy, simplicity and rapidness in operation, high speed and the like and can be used for breeding drought-resistant strains.

The invention discloses a method for determining the biotoxicity of atrazine by utilizing microcystis aeruginosa. The method comprises the following steps: A, cultivating the microcystis aeruginosa; B, preparing microcystis aeruginosa liquid; C, determining the response time of the chlorophyll fluorescence of the microcystis aeruginosa on the biotoxicity of the atrazine; and D, drawing the standard curve for determining the biotoxicity of the atrazine by utilizing the chlorophyll fluorescence of the microcystis aeruginosa, namely adding atrazine standard liquid with series concentration into the preparation liquid of the microcystis aeruginosa, completely mixing until the 'optimum response time' determined by the step C and determining the chlorophyll fluorescence intensity of the microcystis aeruginosa; and setting three parallel samples for each concentration, setting a blank sample by substituting aseptic distilled water for the sample, calculating the photosynthetic inhibition ratio, and finally drawing the 'dose-effect relation curve of atrazine concentration and photosynthesis inhibition ratio' to serve as the standard curve for quantitatively determining the biotoxicity of the atrazine by the chlorophyll fluorescence method of the microcystis aeruginosa. The method can determine the biotoxicity of the atrazine simply, conveniently and quickly, and is low in cost and environment-friendly.

The invention discloses a plant physiology monitoring method based on chlorophyll fluorescence and a multispectral image. The method comprises the following steps that modulated type chlorophyll fluorescence detection is performed on plants to be detected, modulated type chlorophyll fluorescence characteristic parameter diagrams are acquired, and chlorophyll fluorescence parameters related to the plant physiology condition are preset according to the characteristic parameter diagrams; multispectral image measurement is performed on the plants to be detected, and a spectral relative reflectivity parameter diagram of the reflection intensity at the waveband of 540-560 nm to the reflection intensity at the waveband of 500-515 nm is obtained; chlorophyll fluorescence parameter values in a plant area and the spectral relative reflectivity parameter diagram are counted; the plant physiology condition is judged by combining a distribution curve, acquired in the step 3, of the chlorophyll fluorescence parameter values and a distribution curve, acquired in the step 3, of spectral reflectivity parameter values. According to the method, nondestructive testing is performed on the plant physiology by combining a multispectral imaging technology of visible light with a chlorophyll fluorescence detection technology, the method is simple, judging is accurate, and the reliability is high.

The invention discloses a method for predicting wheat grain output based on chlorophyll fluorescence and its model construction method, which solves the technical field of large climate and agricultural model parameters, poor adaptability and large influence on a remote sensing model due to test environment, and low monitoring precision. The method comprises the following steps: information acquisition; screening of sensitive fluorescence parameters, determination of proper space leaf position, establishment of fluorescence potential index; and establishment of an output prediction model. The photopic fluorescence parameters of Fm' and Fv' of two leaves at the top of the wheat are used for better indicating the plant production performance; at a flowering stage and a grouting early stage, the chlorophyll fluorescence Fm' and Fv' potential index can predict the wheat grain output status. The prediction model can effectively reduce the influences by the factors of places, types and weather, has high precision and accuracy on the wheat grain output estimation, and has important meaning and value for guidance of the agriculture production, supply and demand balance of the grains and agriculture policy making.

The invention discloses a method for determining the oleaginous microalgae harvesting time by utilizing the chlorophyll fluorescence parameter Pv/Fm. The method comprises the following steps: (1) carrying out conventional culture and stress induction on oleaginous microalgae, namely carrying out conventional autotrophic culture on the oleaginous microalgae; culturing the oleaginous microalgae to mid-log phase, and inducing accumulation of neutral fat under a stress condition; (2) detecting the chlorophyll fluorescence parameter Fv/Fm, namely sampling in an oil-production inducing process, and detecting Fv/Fm; (3) detecting the content of neutral fat, namely extracting algae oil by chloroform/methanol, and detecting the content of the neutral fat by means of a thin layer chromatography method and image analysis software; (4) determining the harvesting time, namely harvesting microalgae after the neutral fat in cells starts to be remarkably accumulated for 1-2 days, determining the Fv/Fm range after the neutral fat starts to be remarkably accumulated for 1-2 days to be the Fv/Fm harvesting range if the detected Fv/Fm and the neutral fat content are remarkably related, and determining the harvesting time when the algae solution Fv/Fm reaches the harvesting range in induction of neutral fat production under the same stress induction. The method is simple and quick in the chlorophyll fluorescence parameter Pv/Fm detection operation, has few interference factors, and is accurate and stable.

Methods, devices, and systems for measuring carbon assimilation based on simultaneous or near-simultaneous measurements of chlorophyll fluorescence and stomatal conductance of plant. A sample containing chlorophyll, such as a plant leaf, is illuminated with light, e.g., in the form of a single saturating pulse or multiple pulses, and chlorophyll fluorescence and stomatal conductance of the chlorophyll sample are measured. A porometer or infra-red gas analyzer is used to measure stomatal conductance and a photodetector is used to measure fluorescence. A carbon assimilation value for the chlorophyll sample is determined using the measured chlorophyll fluorescence and the measured stomatal conductance.

The invention discloses a detection apparatus and a detection method of photosynthesis of phytoplankton on the basis of chlorophyll fluorescence. The detection apparatus includes an excitation-emission optical structure, an excitation light source driving module, a main control module, and a fluorescent detection module and a light source reference photo-detection module, wherein the structure of the fluorescent detection module and the light source reference light detection module are same. According to the invention, an optical pulse-varying light source is employed with combination of a double-channel signal detection module, quick and accurate measurement of three optical-induced fluorescent dynamic process comprising single-turnover, multi-turnover and relaxation is achieved. By means of analysis on a fluorescent dynamic curve, a plurality detail parameters representing the photosynthesis situation, comprising functional absorption cross section, maximum photochemical quantum efficiency, electron transfer rate and the like of photosynthesis, can be obtained.

This invention provides a plant health diagnostic method and a plant health diagnostic device for detecting initial disorder of a plant individual body carrying pests or the like in a large-scale plant cultivation field. The plant health diagnostic method is for diagnosing a health state of a plant in accordance with chlorophyll fluorescence emitted from chlorophyll of a plant body. The method includes: obtaining a first minimum point s after a maximum point p having largest chlorophyll fluorescence intensity and a first maximum point m after the minimum point s on a time course curve for the chlorophyll fluorescence intensity; defining chlorophyll fluorescence intensity values at the minimum point s and the first maximum point m after the minimum point s as S and M, respectively; and comparing the value S and the value M to evaluate the state of the plant. The state of the plant, which cannot be visually recognized from outer appearance, can be detected by comparison between the chlorophyll fluorescence intensity values (S and M), thereby realizing diagnosis of the state of the plant individual body in an early stage.

The invention discloses a solvent-extraction-and-fluorescence determination method of microalgae lipid content, and belongs to the technical field of microalgae crude lipid determination. The solvent-extraction-and-fluorescence determination method of the microalgae lipid content solves the problems of a fluorimetry in the prior art that dyeing with direct use of Nile red fluorescent staining is halfway and disturbance of microalgae body chlorophyll fluorescence peaks and the like occur when an ultrasound method or a dimethyl sulfoxide (DMSO)-processing-Nile-red-dyeing fluorescence determination method is in use. The solvent-extraction-and-fluorescence determination method of microalgae lipid content comprises the following steps of preparing a standard curve by using microalgae to prepare standard microalgae liquid, extracting microalgae lipid and determining content of the microalgae lipid by using fluorescence. Due to the fact that in a determination process, operation steps are free from evaporation organics, and pollution to the environment is little. The solvent-extraction-and-fluorescence determination method of the microalgae lipid content has the advantages that the solvent-extraction-and-fluorescence determination method of the microalgae lipid content is rapid, simple, effective and sensitive, and determination results are free from influence of retention periods of microalgae samples.

The invention discloses a plant chlorophyll fluorescence detecting device for equalizing irradiation. The plant chlorophyll fluorescence detecting device for equalizing irradiation comprises a light source, a camera, a light filler and a light reflection mirror, wherein the light source is used for transmitting detection light for a to-be-detected sample; the camera is used for collecting light rays from the to-be-detected sample; the light filler is arranged between the camera and the to-be-detected sample; and the light reflection mirror is used for reflecting detecting light from the light source to the to-be-detected sample. The plant chlorophyll fluorescence detecting device for equalizing irradiation provided by the invention can provide three kinds of irradiation including measurement light, saturation pulse light and actinic light; the light emitted by the light source is reflected by the light reflection mirror; and a space with equal irradiation is formed in the device, so that irradiation imaging can be carried out on laminas of plants with different heights.

The invention discloses a multifunctional water bloom growth and extinction experiment research system under influence of hydraulic engineering. The multifunctional water bloom growth and extinction experiment research system comprises a pretreatment system, a flowing water circulation experiment table, a flowing water control system and a monitoring analysis system, wherein the pretreatment system is used for providing algae containing experiment water with different background concentration for the flowing water circulation experiment table; the flowing water circulation experiment table is used for achieving an algae nondestructive growth environment under a smooth continuous water flow environment; the flowing water control system is used for simulating a flowrate variation process of the incoming flow of upstream and downstream hydraulic engineering of a channel reservoir by regulating water supply flow of a water supply channel according to scheduling working conditions of upstream and downstream hydraulic engineering set in the experiment; the monitoring analysis system comprises a water level sensor, a flow velocity meter, a flowmeter, a video sensor, a chlorophyll fluorescence and a computer. The multifunctional water bloom growth and extinction experiment research system can perform quantitative experiment research on complex hydrodynamic condition changes under influence of hydraulic engineering, algae growth changes under flowing water conditions, effect analysis and predication of water bloom inhibition through optical scheduling of upstream and downstream single or step hydraulic engineering, and the like.

The invention discloses a portable intelligent plant physiological state detection system and method. The portable intelligent plant physiological state detection system comprises a lighting module and a camera unit used for collecting fluorescent images of a to-be-detected plant, the lighting module comprises an LED array providing excitation light for lighting and an optical thin film arranged on a light emitting side of the LED array, reflectivity, to fluorescent light of the to-be-detected plant, of the optical thin film is smaller than 5%, and reflectivity, to LED light, of the optical thin film is larger than 95%. By the portable intelligent plant physiological state detection system and method, a user can perform chlorophyll fluorescence detection on plants in field conditions in a portable manner, the fluorescent images acquired by measuring can be processed and analyzed in realtime, a measuring result can be matched with plant physiological states in a database of a remote cloud sharing server in realtime, and updating and machine learning of new samples of the database can be realized.

The invention relates to biotoxicity assessment of drilling fluid, in particular to a method for assessing the acute toxicity of the drilling fluid rapidly by utilizing marine microalgae. The method is as follows: carrying out mixed culture on marine microalgae fluid in a steady growth phase and stock fluid of the drilling fluid for 3-8 hours; sampling, and measuring fluorescence emission spectrum to obtain chlorophyll fluorescence intensity of the algae at the position of 678-685nm; taking the fluorescence intensity of the microalgae without the added stock fluid as contrast, and calculating relative mortality rate of the microalgae; and according to the relative mortality rate of the microalgae, utilizing Curveexpert or Microsoft Excel 2003 software to draw a relation curve between the mortality rate of the microalgae and the concentration of the drilling fluid, and determining whether the drilling fluid is toxic or non-toxic according to the national standard on the basis of 7-8h EC50 value. The assessment result to the toxicity of the water-based drilling fluid by utilizing the microalgae method established by the biotoxicity assessment of the drilling fluid is consistent to that of 96h LC50 of artemia, the time is shortened by 88 hours compared with the conventional 96-hour biotoxicity test time, and the field usage can be achieved.

The invention relates to a method for detecting citrus canker. The method comprises the following steps: carrying out dark adaptation treatment, obtaining fluorescence information and an RGB (Red, Green and Blue) image by using a chlorophyll fluorescence imager and an RGB camera, and computing chlorophyll fluorescence parameters, color features and textural features; distinguishing whether citrus is infected with a disease or not by utilizing a PLS (Partial Least Squares) discriminant analysis model; reading a fluorescence image of a to-be-detected citrus which is infected with the disease, analyzing and computing the area of the disease by applying a Gaussian mixture model, and realizing quantitative analysis on infecting degree. The invention also relates to a device for detecting the citrus canker. According to the method and the device for detecting the citrus canker, disclosed by the invention, citrus fruit canker in different growing periods of a young fruit period, a fruit greening period and a fruit ripening period and in different infecting degrees can be continuously and automatically detected by blending a chlorophyll fluorescence imaging technology with an RGB imaging technology.

The present invention relates to bacteria detection, and flow cytometer detecting process of aerobic anoxygenic photoheterotrophic bacteria (AAPB). The detection process includes the following steps: adding formaldehyde to the sample and culturing; staining the sample with DAPI, collecting forward and lateral scattered light, DAPI fluorescence signal, chlorophyll fluorescence signal and bacterial chlorophyll-a fluorescence signal and separating AAPB from other planktons by means of the difference of signals; separating planktons from other matters by means of DAPI fluorescence signals; separating panktobacteria from other micro planktons by means of the strength of forward and lateral scattered light; eliminating the influence of other matters on the AAPB detection by means of the strength of chlorophyll fluorescence signal; and separating AAPB from other bacteria by means of bacterial chlorophyll-a and obtaining the accurate number of AAPB.

The invention provides a chlorophyll fluorescence automation monitoring system which comprises a sensing unit, a driving unit, a spectrum collecting device and a control unit. The sensing unit is used for collecting solar radiation and vegetation reflected radiation; the control unit is used for controlling the driving unit to drive the sensing unit to move among multiple collecting points, and the control unit is further used for controlling the spectrum collecting device to convert the solar radiation into first spectrum data and convert the vegetation reflected radiation into second spectrum data in different preset integration time during different periods of time and calculating chlorophyll fluorescence data according to the first spectrum data and the second spectrum data.

The invention provides a crop group canopy chlorophyll fluorescence three-dimensional distribution information acquisition method and device. The device comprises a Cropobserver group photosynthetic chlorophyll fluorescence measurement device, a 3D camera and a computer system, wherein the 3D camera is connected with the computer system, Visual studio 2017 and MATLAB 2018 are operated in the computer system, and the Visual studio 2017 calls a point cloud library and a computer vision library to realize three-dimensional visualization of chlorophyll fluorescence information of crops to be detected. According to the invention, the problem of incomplete distribution of acquired two-dimensional chlorophyll fluorescence information is solved, overall 3D visual distribution of crop canopy chlorophyll fluorescence distribution is realized, and important technical support is provided for acquisition and research of three-dimensional visual distribution information of canopy chlorophyll fluorescence of the whole plant.