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Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa

A microcystis aeruginosa, biotoxic technology, applied in material excitation analysis, fluorescence/phosphorescence and other directions, can solve the problems of unstable luminescent bacteria and high method cost, and achieve the effects of low cost, convenient operation and broad application prospects.

Inactive Publication Date: 2013-07-10
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

At present, the luminescent bacteria method is a relatively mature biological toxicity detection method. The toxic effect of atrazine on luminescent bacteria is reflected by the change of the luminous intensity of luminescent bacteria, so as to determine the toxicity of atrazine, but the luminescent bacteria method also has the instability of luminescent bacteria. and the high cost of the method

Method used

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  • Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa
  • Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa
  • Method for determining biotoxicity of atrazine by utilizing microcystis aeruginosa

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Embodiment 1

[0046] 1.1 Culture of Microcystis aeruginosa

[0047] Microcystis aeruginosa belongs to cyanobacteria, and BG-11 medium is used to prepare 100 mL of medium in proportion, and then the medium is put into a sterilizing pot and sterilized at 121 ℃ for 30 minutes. After taking it out, put it into the ultra-clean bench to cool, and inoculate the algae under aseptic conditions. After the inoculation was completed, the cells were cultured in a constant temperature light incubator. The culture conditions were: illuminance of 2000-2500 lux, temperature of 25±1°C, humidity of 75% RH, light-dark cycle of 12h:12h, and static culture. Shake the bottle 2-3 times a day, and change the position of the triangular bottle randomly each time to avoid uneven illumination. The algae grown to the logarithmic phase were transferred to the sterilized medium in proportion, and the transfer was repeated more than 3 times to achieve synchronous growth.

[0048] 1.2 Determination of chlorophyll fluores...

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Abstract

The invention discloses a method for determining the biotoxicity of atrazine by utilizing microcystis aeruginosa. The method comprises the following steps: A, cultivating the microcystis aeruginosa; B, preparing microcystis aeruginosa liquid; C, determining the response time of the chlorophyll fluorescence of the microcystis aeruginosa on the biotoxicity of the atrazine; and D, drawing the standard curve for determining the biotoxicity of the atrazine by utilizing the chlorophyll fluorescence of the microcystis aeruginosa, namely adding atrazine standard liquid with series concentration into the preparation liquid of the microcystis aeruginosa, completely mixing until the 'optimum response time' determined by the step C and determining the chlorophyll fluorescence intensity of the microcystis aeruginosa; and setting three parallel samples for each concentration, setting a blank sample by substituting aseptic distilled water for the sample, calculating the photosynthetic inhibition ratio, and finally drawing the 'dose-effect relation curve of atrazine concentration and photosynthesis inhibition ratio' to serve as the standard curve for quantitatively determining the biotoxicity of the atrazine by the chlorophyll fluorescence method of the microcystis aeruginosa. The method can determine the biotoxicity of the atrazine simply, conveniently and quickly, and is low in cost and environment-friendly.

Description

technical field [0001] The invention relates to the technical field of biological toxicity detection of water environmental pollutants, in particular to a method for measuring atrazine biological toxicity using Microcystis aeruginosa. Background technique [0002] Atrazine (atrazine, atrazine) is a chemical herbicide with the chemical name 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, by Geigy Chemical in 1952 The company developed, applied for a Swiss patent in 1958, applied for a Swiss patent in 1958, and officially put into production in 1959. Because of its low cost and good weeding effect, it has been widely used soon. Atrazine is a selective systemic pre- and post-emergence herbicide, suitable for corn, sorghum, orchards and woodlands, etc. It can control 1-year-old grass weeds and broad-leaved weeds, and also has some perennial weeds. certain inhibitory effect. However, atrazine has a high polarity, is easily soluble in water, and can easily enter the grou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 崔建升徐小惠
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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