7 results about "Dose effect" patented technology

The present application relates to the technical field of machine learning, and particularly relates to a preclinical drug experiment quality evaluation method based on machine learning, comprising: obtaining a plurality of experiment samples, each experiment sample comprising a dose level and an observation value of at least one biological index; determining a trend residual of each biological index of each experiment sample; determining a biological index evaluation weight sequence composed of quality evaluation weights of all biological indexes; determining a composite distance between any two experiment samples; determining a quality evaluation index of each experiment sample; and evaluating the quality of preclinical drug experiments based on the quality evaluation index of each experiment sample. By constructing a dose effect trend function, combining the trend residual and the weight, establishing a composite distance model fusing pharmacological trends, realizing adaptive selection of neighborhood parameters, improving the accuracy and stability of anomaly detection, overcoming the limitations of traditional machine learning LOF algorithm, and enhancing the evaluation reliability.

This invention provides a concentration gradient cell culture system that solves the problem of large errors in manual drug addition and the difficulty in constructing a stable, continuous concentration gradient in studies on the control of osteoclast progenitor cell differentiation using propionic acid-GPR43. [Solution] The system comprises a housing assembly with an inner container, a first liquid storage cylinder 2, a second liquid storage cylinder 3, a tuned crossbeam 4, an adjustable screw rod 5, a flow-dividing branch pipe array frame, a spiral mixing unit, a porous diffusion plate, and a culture tray 9. The tuned crossbeam is driven by the adjustable screw rod to synchronously supply liquid from both liquid storage cylinders. After gradient distribution by the flow-dividing branch pipe array frame and mixing by the spiral mixing unit, the porous diffusion plate forms a continuous and stable propionic acid concentration gradient field on the culture tray. This avoids manual errors, provides accurate concentration gradients to RAW264.7 and BMMs cells in a single experiment, and supports the calculation of dose-effect curves and IC50, as well as the elucidation of the mechanism of osteoclast differentiation control by propionic acid-GPR43 signaling.

This application relates to the field of focused ultrasound, and more particularly to a parameter localization method and kit for inhibiting PCOS-related epilepsy using focused ultrasound. The method includes: acquiring multiple preset power levels, each corresponding to different combinations of power and duty cycle; predicting the safe range of stimulation duration at different power levels based on prior experimental data and a biothermal model; setting several experimental parameter combinations with different preset doses based on the safe ranges; performing ultrasound stimulation on an experimental model based on the experimental parameter combinations and obtaining biological response results; fitting dose-response curves for each power level based on a preset dose-response relationship and the biological response results; determining the optimal dose range for each power level based on the dose-response curves, and evaluating the feasibility of the optimal dose range to determine the optimal parameter combination for focused ultrasound. This method efficiently determines a relatively accurate and effective range of ultrasound parameters while maintaining simple experimental operation, short cycle time, and low cost, providing support for personalized medicine.

The application discloses application of Ms4a1 in peripheral blood leukocytes in calculation of high-energy neutron radiation dose, and relates to the technical field of biological detection. The application focuses on the biological effect and damage mechanism of neutron radiation, and potential biomarkers under dose gradient are screened. The LD50 and LD100 of mice under neutron radiation are determined as 8 Gy and 12 Gy respectively. The damage degree of radiation-sensitive organs such as spleen and intestinal tract is aggravated with dose gradient. The number of lymphocytes in the blood system is significantly reduced with dose, and can be used as an evaluation index of radiation damage. Cytokines such as IL-6 and IL-10 are significantly up-regulated, which indicates the inflammation and immune regulation mechanism. Ms4a1 is finally screened as a potential key gene, and the expression amount of Ms4a1 changes significantly before and after irradiation, and has very high sensitivity. The method can realize rapid and objective estimation of the neutron radiation dose by detecting the expression amount of Ms4a1 in peripheral blood leukocytes and substituting the dose-effect function, and has clinical transformation value.

The application discloses a method for evaluating the hypothalamus-pituitary reproductive endocrine axis interference effect of a compound, and belongs to the technical field of chemical environmental health risk evaluation. The hypothalamic neuron cell line and the pituitary gonadotropin cell line are selected as in-vitro evaluation models, the exposure concentration gradient of the compound affecting cell activity is obtained through cell culture and cytotoxicity experiment, the cell exposure experiment of the hypothalamic neuron cell line and the pituitary gonadotropin cell line is carried out at the exposure concentration gradient, the expression amount of a target gene in the cells is determined after the exposure is finished, the dose-effect curve of the compound interfering with the expression of the target gene is obtained according to the exposure concentration gradient and the expression amount of the target gene, and the interference effect of the compound on the hypothalamus-pituitary reproductive endocrine axis is evaluated by using a toxicology priority index ToxPi.

The application discloses a method for evaluating damage of male sperm quality caused by ionizing radiation, and belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, and determining a mapping relationship between sperm quality rating and average time for recovery of fertility; collecting a semen sample to be measured exposed to ionizing radiation, comparing the semen sample to be measured with the dose-effect database model, evaluating male sperm quality rating, and predicting time required for recovery of fertility based on the male sperm quality rating and the mapping relationship.

The application discloses application of a biomarker combination in peripheral blood leukocytes in calculation of high-energy neutron radiation biological dose, and relates to the field of biological detection. The application focuses on neutron radiation biological effect and damage mechanism, and potential biomarkers under a dose gradient are screened. The LD50 and LD100 of mouse neutron radiation are determined as 8 Gy and 12 Gy respectively. The damage degree of radiation-sensitive organs such as spleen and intestinal tract is aggravated with the dose gradient. The number of lymphocytes in the blood system is significantly reduced with the dose, and can be used as a radiation damage evaluation index. Cytokines such as IL-6 and IL-10 are significantly up-regulated, which indicates inflammation and immune regulation mechanism. Finally, the Ms4a1, CD79a and Ikzf3 combination with extremely high sensitivity is screened. The method can realize rapid and objective estimation of the neutron radiation dose by detecting the expression amount of Ms4a1, CD79a and Ikzf3 in peripheral blood leukocytes and substituting the dose-effect function.