Method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application

A technology of proanthocyanidins and liver cancer cells, applied in the field of medicine, can solve problems such as proanthocyanidins in chestnut that are not involved
CN104774909AInactive Publication Date: 2015-07-15JIANGSU UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2015-07-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application, and the human liver cancer cell HepG2 cell is used for experiment for determination of effects on HepG2 cell proliferation, dose effect and time effect of induction of HepG2 cell autophagic death of proanthocyanidins, content of intracellular ROS (reactive oxygen species and high active oxygen radicals), cell cycle distribution and mitochondrial membrane potential. Results confirm that the proanthocyanidins can induce human liver cancer cell HepG2 cell autophagic death by stimulating of the ROS pathway, and the results are applied to the prevention and treatment of liver cancer.
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Description

technical field

[0001] The invention relates to the field of medical technology, in particular to an analysis method and a new application of proanthocyanidin-induced autophagic death of human liver cancer cells based on an active oxygen pathway. Background technique

[0002] Malignant tumors are one of the three major diseases that seriously endanger human health. Liver cancer is the most common adult malignant tumor, and its mortality rate ranks third in the global cancer mortality rate, and it is still on the rise, with more than 600,000 new cases every year COVID-19 is still one of the biggest diseases threatening the lives of Chinese people. Prevention or intervention is an effective means commonly used clinically to inhibit the progression or reversal of liver cancer. However, chemotherapy drugs, which are the main means of treating malignant tumors, still have many defects, such as high toxicity and side effects and easy drug resistance of tumor cells. In recent yea...

Claims

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