Method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application

A technology of proanthocyanidins and liver cancer cells, applied in the field of medicine, can solve problems such as proanthocyanidins in chestnut that are not involved

Inactive Publication Date: 2015-07-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the production and processing of chestnut pulp, nearly 8.9-13.5% of chestnut shells are still mainly discarded by burning and natural decay, and there are only a few literature reports on the use of chestnut shells to prepare activated carbon, culture media, and adsorb heavy metal ions and pesticides in water.
However, there are very few reports on the chemical constituents of chestnut shells, let alone studies on procyanidins from chestnut shell (CSPCs) and their antitumor activities.

Method used

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  • Method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application
  • Method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application
  • Method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application

Examples

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Embodiment 1

[0046] Example 1 Proanthocyanidins induce autophagic death of human liver cancer HepG2 cells based on reactive oxygen species pathway

[0047] 1 Experimental materials

[0048] 1.1 Test sample

[0049] Crush the chestnut shell, add 0.05‰~0.1‰ sodium bisulfite, seal and extract in boiling water for 0.3-1h, filter the extract with a 200-mesh sieve, and flow the filtrate through the pre-treated macroporous adsorption resin AB- 8 columns, after absorbing non-adsorbed impurities with deionized water, elute the resin column with 70% ethanol to obtain the ethanol eluate, recover the ethanol under reduced pressure below 45°C, and use freeze-drying or spray-drying to obtain light brown chestnut shell proanthocyanidins (procyanidins from chestnut shell, CSPCs). During the experiment, the complete medium was used to prepare the required concentration, and it was prepared for use now.

[0050] 1.2 Human liver cancer HepG2 cells

[0051] Bought from the Institute of Biochemistry and Ce...

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Abstract

The invention discloses a method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application, and the human liver cancer cell HepG2 cell is used for experiment for determination of effects on HepG2 cell proliferation, dose effect and time effect of induction of HepG2 cell autophagic death of proanthocyanidins, content of intracellular ROS (reactive oxygen species and high active oxygen radicals), cell cycle distribution and mitochondrial membrane potential. Results confirm that the proanthocyanidins can induce human liver cancer cell HepG2 cell autophagic death by stimulating of the ROS pathway, and the results are applied to the prevention and treatment of liver cancer.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an analysis method and a new application of proanthocyanidin-induced autophagic death of human liver cancer cells based on an active oxygen pathway. Background technique [0002] Malignant tumors are one of the three major diseases that seriously endanger human health. Liver cancer is the most common adult malignant tumor, and its mortality rate ranks third in the global cancer mortality rate, and it is still on the rise, with more than 600,000 new cases every year COVID-19 is still one of the biggest diseases threatening the lives of Chinese people. Prevention or intervention is an effective means commonly used clinically to inhibit the progression or reversal of liver cancer. However, chemotherapy drugs, which are the main means of treating malignant tumors, still have many defects, such as high toxicity and side effects and easy drug resistance of tumor cells. In recent yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/68G01N21/64A61K31/353A23L1/29A61P35/00A23L33/00
Inventor 段玉清柯佳佳张海晖许慧蔡梅红何远清马海乐
Owner JIANGSU UNIV
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