In vitro fertilization culture solution and preparation method thereof

Abstract

The invention belongs to the field of assisted reproduction and in particular relates to an in vitro fertilization culture solution and a preparation method thereof. A composition used for preparing the in vitro fertilization culture solution comprises resveratrol, reduced glutathione and lecithin, wherein a molar ratio of the resveratrol to the reduced glutathione to the lecithin is (5-50):(100-250):(8000-12000). The in vitro fertilization culture solution disclosed by the invention can achieve effects of providing a stable fertilization condition, removing reactive oxygen produced by cells and reactive oxygen free radicals produced by sperms in the motion process, reducing damage of the reactive oxygen to the sperms and ovum, improving the sperm motility, repairing cellular damage and improving the rate of fertilization and blastocyst formation rate, and the blastocyst formation rate is more than or equal to 90%.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to an in vitro fertilization culture solution and a preparation method thereof. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. [0003] Fertilization of sperm and eggs in vitro requires fertilization medium to provide a stable fertilization environment. Sperm and eggs will produce excessive reactive oxygen species during in vitro culture, which is harmful to in vitro fertilization. The sperm-specific cell structure makes sperm more sensitive to reactive oxygen species and is more likely to be damaged by reactive oxygen species. The harm of active oxygen mainly lies in destroying the morphology of sperm cells and affecting the acrosome reaction during f...

AI Technical Summary

Problems solved by technology

[0005] The nutrients prepared by the semen currently used are incomplete, or their physical or chemical indicators are too wide, and it is easy to cause the death of sperm or egg cells during the culture process, or the lack of sperm capacitation and low viability, or the survival of egg cells is affected, such as in sperm motility. In the test, the rate of sperm viability is low, or its rate is close to or below the acceptable standard (retention rate ≥ 75%)

Some fertilized semen have a high consistency and poor stability. They contain harmful ingredients and inflammatory cells cannot be removed cleanly, which seriously affects the development and growth of fertilized eggs.

[0006] At present, all IVF culture fluids are imported and expensive. In order to fill the domestic gap, assisted reproductive culture fluids are developed to gradually replace imported ones and reduce the cost of assisted reproduction.

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