42 results about "A-type proanthocyanidin" patented technology

The invention discloses a preparation method of proanthocyanidin. In the method, grape seed, pine bark, purple potato or any other plant with high proanthocyanidin content is used as a raw material, an ethanol aqueous solution is used as an extraction solvent, and a double water phase extraction method is utilized to efficiently prepare proanthocyanidin from a crude proanthocyanidin extract. The invention has the advantages of high product yield, high product purity, low solvent consumption, low cost and the like, and can easily implement industrial production.

The invention discloses a method for preparing proanthocyanidins liposomes, and relates to a method for preparing liposomes. The method solves the problems of large grain size and difficult absorption of a human body by protecting proanthocyanidins in a microcapsule form in the prior art. The method comprises the following steps of: 1, putting soya bean lecithin and cholesterol in a pear-shape flask, adding mixed solution of diethyl ether and absolute ethyl alcohol, adding proanthocyanidins and phosphate buffer, and mixing to obtain mixed solution; 2, performing ultrasonic processing on the mixed solution in a 25 DEG C water bath, performing rotary evaporation to form a film in a water bath at the temperature of between 30 and 50 DEG C and under the pressure of between 400 and 700MPa, adding phosphate buffer again, and performing hydration to obtain coarse suspension; and 3, and filtering the coarse suspension by using a filter membrane of 0.45 mu m. The liposomes are adopted for encapsulating the proanthocyanidins, so the proanthocyanidins are more stable, have small grain size and target, and are favorable for absorption of the human body; the encapsulation rate of the proanthocyanidins liposomes is 88.89 percent; and the preparation process is simple.

The invention relates to a proanthocyanidin effervescent tablet and a method for preparing the same. The effervescent tablet consists of the following components in the following weight percentage: 2 to 15 percent of proanthocyanidin, 1 to 10 percent of vitamin C, 10 to 40 percent of beta-cyclodextrin, 20 to 50 percent of citric acid, 30 to 35 percent of sodium bicarbonate, 0.5 to 1 percent of protein sugar, 0.1 to 2 percent of flavoring essence, and 0.05 to 0.1 percent of magnesium stearate. The proanthocyanidin effervescent tablet has the advantages that the proanthocyanidin effervescent tablet can be conveniently carried around, used and absorbed as the common effervescent tablets, and simultaneously has characteristics of high concentration of functional substances and strong functionality, as well as bright color of the proanthocyanidin; the proanthocyanidin effervescent tablet has the greatest advantage that the stability of the proanthocyanidin is greatly improved after the proanthocyanidin is embedded by the beta-cyclodextrin; the vitamin C is added in the effervescent tablet to further prevent the proanthocyanidin from being oxidized and act synergistic effect on the antioxidant activity of the proanthocyanidin so as to improve the functionality thereof; furthermore, the proanthocyanidin effervescent tablet has simple formulation, easy preparation, and low cost.

The present invention relates to a procyanidins health beer production method using leftover grape seeds of a wine plant for brewing. The procyanidins health beer production method is as follows: crushing of a raw material of the leftover grape seeds of the wine plant, addition of grape seed powder in boiling stage and yeast-rising stage, and determination of procyanidins in health beer and determination of DPPH free radical removal capability of the procyanidins health beer. The procyanidins health beer production method has the advantages of simple process, low cost and easy industrial production, and the like, and the obtained procyanidins health beer has high procyanidins content, and has strong free radical scavenging capability and antioxidant health-care effect.

The method of extracting natural dye from plane tree is to extract natural active dye from bark of plane tree containing resorin A type proanthocyanidin in 22-27 % possessing dyeing activity in the molecular weight range of 350-2000. By means of extracting, adsorbing, refining and other technological steps, coffee dye and pale yellow dye of purity over 90 % are prepared. The obtained dyes have high dyeing performance and high fastness on hair, wool and fabric, and will have raised use effect when being compounded with hydrolyzed tannin or flavone glycoside dye. The obtained dyes are used in dyeing hair, wool, fabric, etc.

The invention discloses a procyanidin capsules and a process for manufacturing the same. The capsules are manufactured by taking procyanidin and astragalus polysaccharide as main materials and caseinphospho peptides or D-mannitol, magnesium stearate and gelatin as auxiliary materials. The capsule has the advantages of being conveniently taken, avoiding absorbing moisture and having functions of immunoregulation, anti-ageing and radiation hardening, protecting cardiovascular health by removing free radicals in vivo, and preventing crosslinking of skin collagens, inhibiting pigmentation, dispelling wrinkles and whitening the skin, keeping skin elasticity and delaying ageing.

The invention relates to proanthocyanidin sustained release nano-micro spheres. The proanthocyanidin sustained release nano-micro spheres comprise 1-5 parts of proanthocyanidin, 1-12 parts of chitosan or derivatives thereof, 1-12 parts of phospholipid or derivatives thereof and 1-10 parts of excipients, wherein the chitosan and the derivatives thereof are one or more of high, medium and low-molecular weight chitosan, chitosan oligosaccharide, chitosan quaternary ammonium salt and carboxymethyl chitosan. The particle size of the proanthocyanidin sustained release nano-micro spheres is 10nm-0.3 mu m; the proanthocyanidin is prepared into the powder sustained release nano-micro spheres, and the proanthocyanidin sustained release nano-micro spheres can directly play a role in the lung as a pulmonary inhalation medicine-carrying formulation, improve the bioavailability of the proanthocyanidin and reduce the dosage by sustained release; the proanthocyanidin nano-micro spheres can also be applied as an oral sustained release formulation; the proanthocyanidin nano-micro spheres have appropriate particle size and morphology and other powder properties, as well as sustained release; furthermore, the preparation method has the advantages of simplicity and convenience in operation, stable process and easiness in industrial production.

The invention discloses procyanidine-modified carboxymethyl chitosan, a preparation method thereof, and application thereof in the fields of medical materials, health-care foods, cosmetics and the like. The procyanidine-modified carboxymethyl chitosan keeps the characteristics of carboxymethyl chitosan and has high oxidation resistance, antibacterial activity, barrier properties and the like, and is worthy of being applied.

The invention relates to a procyanidine effervescent tablet and a preparation method thereof. The preparation method comprises the following steps: using a Hua Niu apple procyanidine extract as a main raw material; adding a disintegrating agent, an excipient, a flavoring agent and food color, wherein the additives all meet the food sanitation and safety requirements; adopting a direct powder tabletting technology to form the procyanidine effervescent tablet with the certain shape. Procyanidine is a main polyphenol compound combined from different amounts of catechin or epicatechin, and research shows that the procyanidine has the pharmacological functions of protecting heart, improving study and memory functions of mice with study and memory impairments, resisting oxidation, preventing and treating cancer, adjusting blood fat, promoting hair growth and the like. According to the research, the extraction process of procyanidine in apple peel residue is optimized, procyanidine is developed into the procyanidine effervescent tablet, and not only can full utilization of resource be realized, but also a novel health food is additionally provided, so that the health condition of people and the life quality are improved.

The invention provides a procyanidine soft capsule, which comprises contents consisting of procyanidine, plant oil, beewax and phospholipid according to mass percentage: 10 percent to 60 percent of procyanidine mixtures, 35 percent to 87.5 percent of plant oil, 0.5 percent to 2.5 percent of beewax and 1 percent to 5 percent of phospholipid. The procyanidine soft capsule can be prepared from the procyanidine mixture, the plant oil, the beewax, the phospholipid and other human body acceptable medical auxiliary ingredients through a pressing method. The procyanidine soft capsule provided by the invention has the advantages that the production cost is low, and the production process is simple, convenient and advanced. The appearance of the procyanidine soft capsule is attractive, the carrying is convenient, the dosage is small, one capsule is taken in one day, and the taking compliance is good. The procyanidine soft capsule is particularly suitable for middle-aged people and old people and has good anti-oxidation health-care effects.

The invention discloses a method for analysis of proanthocyanidins induced liver cancer cell autophagic death and application, and the human liver cancer cell HepG2 cell is used for experiment for determination of effects on HepG2 cell proliferation, dose effect and time effect of induction of HepG2 cell autophagic death of proanthocyanidins, content of intracellular ROS (reactive oxygen species and high active oxygen radicals), cell cycle distribution and mitochondrial membrane potential. Results confirm that the proanthocyanidins can induce human liver cancer cell HepG2 cell autophagic death by stimulating of the ROS pathway, and the results are applied to the prevention and treatment of liver cancer.

The invention relates to a proanthocyanidin derivative which is prepared by carrying out acetylation reaction on proanthocyanidin extracted from grape seeds and acetic anhydride. The preparation method comprises the following steps: pulverizing fresh grape seeds, degreasing, adding a 60% ethanol water solution as an extractant, carrying out vibrating extraction in an ultrasonic extracter for 20 minutes, repeating the extraction process three times, concentrating the volume, carrying out low-temperature centrifugal filtration, extracting with ethyl acetate three times, distilling the extract with a rotary evaporator, concentrating to obtain reddish brown proanthocyanidin, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin;Or extracting at 50 DEG C four times (1 hour a time) by using a 60% ethanol water solution as an extractant, concentrating the extract, carrying out low-temperature centrifugal filtration, extracting with twice of ethyl acetate, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin. The proanthocyanidin derivative is used for preparing health foods and medicines for preventing and treating tumors.

The invention discloses medicinal application of A type proanthocyanidin trimer polyphenol. The medicinal application is that the A type proanthocyanidin trimer polyphenol is taken as one of active compositions or the only one active composition to prepare medicine preparations for treating immunologic diseases. Experiments show that the half cell lethal concentration of the A type proanthocyanidin trimer polyphenol is larger than 50 mu g/mL, and the A type proanthocyanidin trimer polyphenol has inhibiting effect on propagation of lymphocyte induced by mitogen ConA and secretion of cytokines IL-6 and IFN-gamma important for immunoloregulation and inflammation reactions, is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating immunologic diseases, especially is expected to be taken as one of active compositions or the only one active composition for preparing medicine preparations for treating rheumatoid arthritis, and has medicinal value.

The invention discloses an analysis method for metabolin of A-type proanthocyanidins, which comprises the steps of collection of a biological sample, preparation of metabolin solution, detection of the metabolin and the like, wherein the preparation of the mtabolin solution comprises preparation of hydrolysate, preparation of crude extraction of the metabolin and purification of the metabolin crude extraction. The analysis method for the metabolin of the A-type proanthocyanidins is good in separating purification effect, efficient, fast, high in result accuracy and good in reproducibility, and not only facilitates understanding of a metabolic mechanism and the metabolin, but also plays an important role in further utilization of the A-type proanthocyanidins.

The invention discloses application of proanthocyanidin to preparation of a functional food or medicine for improving the sperm quality of obese men and also discloses application of a pharmaceuticalcomposition taking the proanthocyanidin as an active component, and a pharmaceutically acceptable additive to preparation of the functional food or medicine for improving the sperm quality of the obese men. A research proves that the proanthocyanidin has an obvious reduction effect on a weight gain rate of rats fed by high fat diet, and has the effects of improving the level of serum testosterone,protecting the structural integrity of testicles, improving the spermatogenesis obstruction caused by obesity, reducing excessive apoptosis of spermatogenesis cells of the testicles, improving the quantity and vitality of sperms and reducing the distortion rate of the sperms, so that the provided pharmaceutical can be used for improving the sperm quality of the obese men and protecting a reproductive function of the obese men. The invention digs novel medical application of the proanthocyanidin and provides meaningful reference for solving the reproductive dysfunction of the obese men.

The method relates to the fields of plant extraction and healthcare foods, and especially relates to a method for extracting and purifying procyanidine from grape seed with macroporous absorption resin. The method comprises the specific processes of resin pretreatment, grape seed extract liquid preparation, and grape seed extract liquid purification. The grape seed extract liquid purification process comprises macroporous resin purification and chromatography resin purification. With the method, a finished product requirement of high purity can be achieved with the two-stage purification operation; in all processes, a basic concept of procyanidine high-purity product dependence on organic solvent is avoided to a maximal extent, and the extract liquid is completely purified with resin, such that residue caused by the application of organic solvent is avoided. The invention provides the green and environment-friendly procyanidine high-purity extraction method which is suitable for actual productions.

The invention discloses a procyanidine extraction method. The extraction method comprises the following steps: 1) taking washed and dried grape seeds and grape skin, and uniformly mixing the grape seeds and grape skin according to the proportion of 12-15:2-6, fragmenting the mixture to powder; 2) adding powder in the step 1) in a mixing extract, wherein the mixing extract is formed by uniformly mixing ethanol, ethyl acetate and water according to volume ratio of 12-16: 6-9: 3-5; and uniformly stirring the materials; and 3) performing ultrasonic extraction on the mixture in the step 2) for 30-50 min at condition of 8-10 MPa, 3-3.5 MHz ultrasonic wave and at 15-22 DEG C, quickly cooling the material to room temperature, filtering the material, and performing vacuum drying on a filtrate. The method employs waste material grape seed and grape skin which cannot be directly eaten, the waste material grape seed and grape skin are taken as the preparation raw materials of procyanidine, the waste can be used, cost is saved, under effect of a mixed solvent, the procyanidine can be dissolved, and is extracted under high pressure and low temperature through ultrasonic wave, the procyanidine is difficulty oxidized, the extraction efficiency is high, impurity is little, and the speed is fast.

The invention discloses a procyanidin nanoemulsion drugs the composition of which is procyanidin, non-ionic surfactant, oil, deionized water, its preparation method is surfactant is loaded and weighed according to the proportion formula, choosing oil phase, and make sure that the HLB for its emulsion is close to the HLB of surfactant, then well prepared surfactants and oil phase is mixed according to the original proportion formula, adding proportional anthocyanin, blending and stirring completely, slowly dropwising deionized water and stirring at room temperature until it has similar viscosity with water and become clear transparent solution which is procyanidin nanoemulsion drug. Nano-procyanidin significantly improves the solubility, dispersion of procyanidins in water and its bioavailability, improves the stability of procyanidins. The nano-emulsion can be widely used in cosmetics, pharmaceuticals, beverages, health care products, fodder and other fields.

The invention provides a feeding method of proanthocyanidins on horses. The feeding method includes: adopting coarse feed and concentrated feed for mixed feeding, wherein the proanthocyanidins is mixed in the concentrated feed, and the feeding amount of the proanthocyanidins per kilogram of the body weight of the horses is 40-60mg/kg. The reasonable proanthocyanidins feeding method on the horses is provided for healthy culture of the horses, and daily health and competition demands of the horses are ensured.