Preparation method and application of proanthocyanidin derivative
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A technology of proanthocyanidins and derivatives, which is applied in the field of medicine, can solve the problem of low solubility, and achieve the effect of facilitating absorption, good treatment and prevention of tumors
Inactive Publication Date: 2014-06-04
XINXIANG MEDICAL UNIV
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[0005] The object of the present invention is to provide a preparation method of proanthocyanidin derivatives in order to overcome the disadvantage that the current proanthocyanidin dimers and above multimers have low solubility in water at room temperature
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Embodiment 1
[0053] After the fresh grape seeds are processed, the grape seeds are collected and air-dried naturally. The dried grape seeds are crushed to 30-40 mesh with a pulverizer.
[0054] Weigh 1 part of 100g of the above-mentioned treated filter residue, add it to a 500mL Erlenmeyer flask, degrease with petroleum ether on a Soxhlet extractor for 8 hours, remove fat-soluble pigments, filter with suction, and remove solvent and grease. Add 60% ethanol aqueous solution for extraction, the ratio of solid to liquid is 1:4, shake and extract in an ultrasonic extractor for 20 min, repeat the extraction three times, and combine the extracts. When the extract was concentrated to half of its original volume, a certain amount of saturated NaCl solution was added. After low-temperature centrifugal filtration, it was extracted three times with 2 times the amount of ethyl acetate. The extract was distilled and concentrated with a rotary evaporator to obtain reddish-brown proanthocyanidins.
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Embodiment 2
[0057] After the fresh grapes are processed, the grape seeds are collected and dried naturally. The dried grape seeds are crushed to 30-40 mesh with a pulverizer.
[0058] Degreasing: in order to realize the comprehensive utilization of grape seeds, add petroleum ether, grape seeds: petroleum ether (weight / volume) = 1: 3 normal temperature soaking 48h, stirring intermittently. Suction filtration to remove solvent and grease.
[0059] The degreased grape seed powder was dried in the shade, stirred with 5% NaCI solution at a ratio of 1:5 to liquid for 1 h at room temperature to remove the protein therein, filtered with suction, washed with distilled water, and the filter residue was dried in the shade for later use.
[0060] Weigh 1 part of 100 g of the above-mentioned treated filter residue, use 60% ethanol aqueous solution as the extractant, the particle size of the raw material is 40-60 mesh, the ratio of solid to liquid is 1:12, and the extraction temperature is 50 ° C, and...
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Abstract
The invention relates to a proanthocyanidin derivative which is prepared by carrying out acetylation reaction on proanthocyanidin extracted from grape seeds and acetic anhydride. The preparation method comprises the following steps: pulverizing fresh grape seeds, degreasing, adding a 60% ethanol water solution as an extractant, carrying out vibrating extraction in an ultrasonic extracter for 20 minutes, repeating the extraction process three times, concentrating the volume, carrying out low-temperature centrifugal filtration, extracting with ethyl acetate three times, distilling the extract with a rotary evaporator, concentrating to obtain reddish brown proanthocyanidin, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin;Or extracting at 50 DEG C four times (1 hour a time) by using a 60% ethanol water solution as an extractant, concentrating the extract, carrying out low-temperature centrifugal filtration, extracting with twice of ethyl acetate, and carrying out acetylation reaction with acetic anhydride to obtain the acetyl proanthocyanidin. The proanthocyanidin derivative is used for preparing health foods and medicines for preventing and treating tumors.
Description
Technical field: [0001] The invention belongs to the technical field of medicine, and relates to the preparation method and application of proanthocyanidin derivatives, in particular to its application in the preparation of anti-tumor health food and medicine prescriptions. Background technique: [0002] Proanthocyanidins, often called procyanidine or proanthocyanidins abroad, are natural phytochemicals formed by the polymerization of different amounts of catechin or epicatechin monomers. They are widely found in various plants such as oranges, lemons, olives, and grapes. Among them, it has good anti-oxidation and free radical scavenging effects, and has many applications in food, health products, pharmaceuticals and other fields. Among them, grape seeds are easy to preserve and process, and are rich in proanthocyanidins, so in the prior art, grape seeds are mostly used as raw materials to extract proanthocyanidins. Grape seed proanthocyanidins contain 2-10 catechin monomer...
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