466results about How to "Extract completely" patented technology

The invention discloses a method for preparing tobacco sheets and an application of the method. In the method, enzymolysis is implemented when the tobacco stalk and the tobacco powder are soaked, solid-liquid separation is implemented after extraction, the solids are made into fiber sheet substrates, liquid is made into concentrated solution by alcohol precipitation and concentration, the concentrated solution is made into coating liquid by maillard reaction and coated on the fiber sheet substrates, and the fiber sheet substrates are dried and cut into tobacco sheets. In the invention, the macromolecular compounds that influence the quality of the tobacco sheets are sufficiently degraded by enzymolysis, the content of the impure taste in the tobacco sheets is decreased, the internal quality of the tobacco sheets is improved, and better aroma is obtained by the technical solution that produces prospective aroma by the maillard reaction to provide a rich aroma for the tobacco sheets so as to obtain tobacco sheet products fitting different types of cigarette products and having better internal quality. The tobacco sheets prepared by the method have excellent compatibility and harmonywith the conventional raw materials of the cigarettes, and have the advantages of good cigarette taste, various and rich aromas and less impure taste.

The invention discloses a method for extracting peony seed oil. In the method, peony seed powders are put in a supercritical CO2 extractor for supercritical extraction, and oil is collected to obtain the provided peony seed oil. In the method, the supercritical extraction temperature ranges from 30 to 50 DEG C, 45 DEG C is optimized, the pressure for supercritical extraction ranges from 20 to 35MPa, and 30MPa is optimized; time for supercritical extraction ranges from 0.5 to 2 hours, and 1 hour is optimized. The grain diameter of the peony seed powders ranges from 20 to 60 screen meshes. The provided supercritical CO2 extracting method for preparing peony seed oil needs no organic reagents, has simple and convenient extracting and separating method, the extraction yield can reach 27 percent, and obtained unsaturated fatty acid can reach 90 percent; and compared with the prior organic solvent extraction method, the contents of linolenic acid and linoleic acid in peony oil are increased to certain extent, and the extraction is completer.

The invention discloses a preparation method of traditional Chinese medicine ultrafine formula particles. The preparation method comprises the following steps that: single Chinese medicinal material or traditional Chinese medicine decoction pieces are partially ultrafine-crushed into ultrafine powder, the other parts are coarse-crushed, extracted, concentrated, dried and crushed to obtain dry extract powder, the ultrafine powder and auxiliary materials are added to obtain mixture, and the obtained mixture is mixed uniformly, palletized, granulated and subpackaged. The traditional Chinese medicine ultrafine formula particles is characterized in that the characteristics of the traditional Chinese medicine ultrafine decoction pieces of saving medicine materials and being controllable in quality and convenient in administration. Compared with the traditional Chinese medicine formula particles, the preparation method better preserves the medicinal properties of the traditional Chinese medicines, has the advantages of little used amount of auxiliary materials, convenient identification, benefit to measuring the content of index ingredients, preserves the advantages that the traditional Chinese medicine decoction piece can be added and reduced according to the disease and the traditional decoction has unique curative effect, also overcomes the defects of troublesome decoction and inconvenient administration, and has advanced technique and controllable quality. The invention also discloses a processing method of the Chinese medicinal material, which extracts the raw medicinal materials partially, carries out ultrafining treatment partially and then carries out mixing. The method can obvious improve the utilization ratio and leachability of the Chinese medicinal materials.

The invention relates to a method for detecting alkaloid in tobacco. The method comprises steps that: step 1, a tobacco sample is added to an alkaline solution; the mixture is subject to an ultrasonic treatment; an organic solvent containing quinoline is added to the mixture, and the mixture is processed through oscillation extraction; an organic layer is condensed, such that a sample solution is obtained; step 2, the sample solution is detected by using a gas chromatograph-mass spectrometer, such that alkaloid in tobacco is processed through quantitative and qualitative determinations. The detection method provided by the invention is advantaged in simple steps, convenient operation, good reproducibility, high sensitivity, and high recovery rate. With the method provided by the invention, all the components of tobacco alkaloid can be completely extracted. Therefore, the method can be effectively used in analysis and detection of tobacco alkaloid.

The invention discloses a composite plant beverage which is water extract fluid obtained by the extraction and separation with water after complex formulation of traditional original Chinese plant herbal medicine materials, and contains the following traditional Chinese plant herbal medicine materials according to the parts by weight per 1000 volume parts of beverage: 15-30 parts of indigowoad flower, 8-16 parts of chrysanthemum, 8-16 parts of honeysuckle, 5-15 parts of tea, 8-12 parts of fructus momordicae, 8-12 parts of rhizoma imperataem, 5-10 parts of balloon flower, 1-5 parts of rhizoma puerariae, 1-5 parts of glycyrrhiza and 1-5 parts of mint. The extraction and condensation in the processing of the beverage, both avoids from using high temperature, thus not only leading the extraction to be complete but also not destroying the active component. The composite plant beverage has the efficacies of clearing heat, nourishing and strengthening the immunization and the like.

The invention discloses a bee pollen beverage and a preparation method thereof, which relates to a honey product beverage, in particular to a health care beverage taking the bee pollen as raw material and the preparation method thereof. The invention provides a desirable method for a pollen composite temperature difference wall broken technique, which has a wall broken rate of higher than 95, simple technological operation, and short production period, and can avoid the pollution of other bacteria, eliminate safety hazards and extract complete nutrient contents; and also provides a preparation method for pollen and bee pollen latex. The bee pollen beverage of the invention adopts bee pollen latex or the composition of bee pollen latex and pollen latex as leading material, thus providing for people a bee pollen beverage which has rich and complete nutriment, easy absorption and fine taste.

The invention discloses a method for qualitatively detecting polychlorinated biphenyl in soil and / or sediment. The method comprises the following steps of: (1) extracting the soil and / or sediment to be detected by adopting an accelerated solvent extraction method, thereby acquiring an extracting solution; (2) purifying the extracting solution through a gel permeation chromatograph, thereby acquiring an extract; (3) eluting the extract through a solid phase extraction column, and collecting the eluent; and (4) analyzing the eluent by a gas chromatograph-mass spectrometer so as to acquire full scan data, and then performing deconvolution analysis on the acquired full scan data according to a retention time locking database in the gas chromatograph-mass spectrometer, thereby qualitatively detecting the polychlorinated biphenyl in the soil and / or water body sediment to be detected.

The invention relates to a process for producing ginkgo extract and belongs to the technical field of plan extraction. The process comprises the following steps of: adding ginkgo leaves serving as raw materials into 50 to 70 percent ethanol of which the weight is eight times that of ginkgo leaves serving as the raw materials to perform leaching three times, wherein the leaching temperature is 70 DEG C; collecting extracting solution, evaporating the extracting solution under vacuum, recovering ethanol, centrifuging the extracting solution and collecting supernatant solution; and subjecting the extracting solution to the centrifugal treatment by a macroporous resin chromatographic column of which the diameter-to-height ratio is 1:10, leaching the extracting solution in turn with 5 to 15 percent ethanol until the eluate is clear and bright, desorbing the extracting solution with 75 percent ethanol, after desorption, collecting desorbing solution, allowing the desorbing solution to pass through a macroporous resin chromatographic column of which the diameter-to-height ratio is 1:30, collecting the desorbing solution, decompression-recovering ethanol, condensing the desorbing solution into thick paste, and vacuum-drying the thick paste to obtain the ginkgo extract. The process adopts multiple leaching and multistage centrifuging, and also adopts a double-time desorbing method of coarse extraction and refined extraction, so that the contents of the active components are greatly improved; and the ginkgo extract with different ratios of active components can be prepared as required.

A fluidic tile having a first substrate containing macrofluidic structures bonded to a second substrate containing microfluidic structures. The microfluidic structures correspond to the macrofluidic structures in the first substrate and provide fluid flow paths between the macrofluidic structures. One of the microfluidic structures is a washing siphon that provides a fluid flow path between a purification chamber and a waste chamber. The washing siphon is configured to be primed when a volume of liquid in the purification chamber exceeds a predetermined amount causing the washing siphon to initiate transfer of the liquid in the purification chamber to the waste chamber when the volume of the liquid in the purification chamber exceeds the predetermined amount.

The invention relates to rhodococcus ruber for generating natural haematochrome and a preparation method and application thereof, wherein the haematochrome is generated through microbial fermentation; the strain category belongs to rhodococcus ruber; the preservation numbr is CGMCC No.12604. The invention also relates to a preparation method of the fermentation natural haematochrome. The fermentation strain of rhodococcus ruber CGMCC No.12604 is used for inoculating the cultured seeds into a fermentation culture medium according to the inoculation quantity according to the inoculation quantity of 1 percent to 10 percent (v / v), wherein the culture temperature is 30 to 40 DEG C; the fermentation ventilation quantity is 5 to 13m<3> / h; the tank pressure is 0.02 to 0.09 MPa; the stirring speed is 120 to 150 r / min; the fermentation time is 120 to 144h; in the fermentation process, sterile air is firstly introduced; the pH of the fermentation liquid is maintained at 6.5 to 7.5 by ammonium hydroxide or alkali liquor; according to the fermentation result, the yield of the haematochrome is 1 to 2g / L. The invention also discloses an extraction method of the rhodococcus ruber haematochrome.

The invention provides a blueberry leaf health care beverage. The beverage comprises the following materials in weight portion: 30 to 50 portions of blueberry leaf, 10 to 30 portions of blueberry fruit, 5 to 10 portions of seabuckthorn fruit, 10 to 20 portions of rose hip and 10 to 20 portions of cordyceps militaris, and active ingredients of the materials are extracted by microwave technique to prepare the beverage. In all materials, vitamin C is abundantly contained, particularly, cyanidin composition and flavonoid contained in the blueberry leaf can delay ageing, improve the immunity of human body and obviously improve eyesight of people and has a plurality of physiological activities such as blood sugar and blood fat adjustment, and the like; the cyanidin composition is a natural bacteriostat, colorants and bacteriostats are not necessary to be added, and flavoring agents such as citric acid are not necessary to be added to regulate the acidity, which is more helpful to the health of human body. By the verification of experiments and the experience of people, the health care beverage is easily absorbed by human body and taken conveniently, and can obviously improve the eyestrain of human body; moreover, the health care beverage also has the characteristics of unique formula, reasonable composition, simple process, body building-up, remarkable function and the like.

The invention relates to a millettia speciosa champ health drink and a preparation method thereof which is characterized in that the millettia speciosa champ is singly used or matched with one or multiple from galangal fruit, Morinda officinalis, barrenwort, liquorice, antler glue, the kidney of a yellow dog, tripterygiumforrestii, ficus radicals and banlangen; hot water is adopted for extractionor ultra-extraction to separate the effective components in the millettia speciosa champ or the mixture thereof to the largest extent; then the millettia speciosa champ or the mixture thereof is stewed and filtered; a natural health agent of honey and citric acid are added for seasoning, filtering, sterilizing, filling and cooling, thus obtaining a special good health drink. The millettia speciosa champ health drink prepared by the invention has uniform color, has the special fragrance of the millettia speciosa champ, has no impurities as well as has the remarkable effects of supplementing deficiency, nourishing lung, strengthening gluten, activating as well as strengthening body.

Disclosed are instant functional maca coffee and a production method thereof. The instant functional maca coffee comprises, by weight, 5-40% of functional maca extract, 50-80% of instant coffee powder, 1-10% of white granulated sugar, 0.5-5% of non-dairy creamer, 1-10% of zinc lactate, and 0.1-2% of vitamin B1. The instant functional maca coffee retains coffee taste and gives full play to functions of maca, such as fatigue relief, radiation proofing, immunity enhancement, hormone balancing, benign prostatic hyperplasia proofing and sexual function enhancement. Bioavailability of functional components of the functional maca extract is improved, and accordingly the instant functional maca coffee has broad application prospect in terms of preventing and improving sub-health.

The invention discloses a high- purity of osthol, structure proof with spectral analysis, method for preparation, stability study, acute toxicity study, the drug compositions containing osthol, and new drug utilities of the natural product. By the technique steps of extracted Chinese pharmacy conidium fruit with alcohol, decolorizing and enriching by large-hole adsorption resin, and separating and purifying, it can prepare osthol with a purity of above 98%. The study expresses that the osthol has a clear utility of anti-cancer, anti-auto lymphoma, and so on.

The preparation process of alive adult roach extract includes the steps of: soaking alive roach in ethanol solution of 60-85 % concentration until death; soaking the roach in ethanol solution of 30-95 % concentration inside a sealed container, filtering the soaked liquid to obtain filtrate, concentrating the filtrate to obtain extractum while recovering ethanol; water extracting soaked roach via decoction for 2 or 3 times, filtering and vacuum concentration to obtain extractum; mixing the extractum obtained in the two steps; vacuum drying, and crushing to obtain powder. The product may be used in preparing some medicine for clinical application.

The invention provides a method for preparing a uranium adsorption material with an intercalation structure by use of oil shale ash as a raw material. The method comprises the following steps: crushing and grinding the oil shale ash to 100 meshes, adding NaCl accounting for 30 to 60% of oil shale ash by mass, and calcining for 3 to 10 hours at a temperature of 800 to 1400 K and air drying; adding an acid solution by 2 to 20 mL per gram of the oil shale ash, and after extraction, carrying out residue separation to obtain an extraction solution; through a method of adding metal ions, adjusting the ratio of the total mole amount of three-valent and four-valent matal ions to the total mole amount of divalent metal ions in the extraction solution to be 1:5-1:2, adjusting the pH of the solution to be 5-12, and further stirring for 10 to 1000 min or hrdro heating for 120 to 3600 min by transferring to a hydro-thermal kettle at a temperature of 373 to 433 K; and filtering, washing and drying to obtain the uranium adsorption material with the intercalation structure. The preparation method provided by the invention is simple in preparation technology and low in production cost, and is free from the restrictions of chemical composition differences of the oil shale ash, thereby facilitating the harmlessness, resource and functionalization utilization of industrial waste, i.e, the oil shale ash.

A process for preparing high-purity astragaloside from astragalus root includes such steps as extracting, concentrating, removing impurities, hydrolytic conversion, extracting in solvent and purifying.

The invention relates to a cosmetic containing natural plant extractives and a preparation method thereof, belonging to the technical field of cosmetics. The cosmetic containing natural plant extractives is characterized by being prepared from the following components in percentage by weight: 1.0-2.2 percent of okra fruit extractive, 1.0-2.0 percent of grape seed extractive, 0.5-1.2 percent of sapindus extractive and the balance of aid and carrier. The favorable synergy and excellent efficacy in aspects of improving skin winkles, whitening and beautifying skin, clearing radicals, lightening speckles and decomposing melanin is presented due to simultaneously using the okra fruit extractive, the grape seed extractive and the sapindus extractive, and the favorable antimicrobial preservative action of the sapindus extractive ensures that the microorganism index of the product can accord with the cosmetic production requirements.

The invention discloses an HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar, belonging to the technical field of food sugar detection. The method comprises the following steps: firstly, juicing and homogenizing grapes, ultrasonic extracting the juice, and then, centrifuging the juice, taking the supernatant, filtering the supernatant and using the filtrate as a test sample; respectively preparing standard working solutions with a series of concentrations of glucose, fructose and sucrose; the chromatographic conditions are as follows: a polystyrene-divinyl benzene bonded amino sugar column serves as a chromatographic column, the mobile phase is a mixture of acetonitrile and water with volume ratio of 70-80:30-20, and the flow velocity is 1mL / min, the column temperature is 30-35 DEG C, the detector is ELSD, the temperature of a detecting pool is 40-80 DEG C, and the sample size is 10 microliters; and then inspecting linear dependence and precision, and detecting stability and sample recovery. The method provided by the invention is simple and convenient to operate, small in time consumption, good in measurement result repeatability, high in precision and capable of accurately and quickly measuring the contents of glucose, fructose and sucrose in grape berry.

The invention relates to a production technology of grape pip and grape skin extract, belonging to the technical field of plant extract. The production technology comprises the following steps: adding the raw materials of grape pip and grape skin into 50-70% of alcohol the volume of which is eight times of the raw materials; extracting the grape pip and grape skin at the extracting temperature of 70 DEG C for three times; collecting the extraction solution, vacuum evaporating, recovering the alcohol and collecting supernate after centrifugation; carrying out chromatographic column on the centrifuged extraction solution in macroporous resin with the diameter height ratio of 1:10; eluting with 5-15% of alcohol in batch until the eluate is clear; desorbing with 70% of alcohol, collecting the desorption solution after desorption; carrying out chromatographic column on the centrifuged extraction solution in macroporous resin with the diameter height ratio of 1:10; collecting the desorption solution, decompressing and recovering the alcohol, concentrating into thick paste, and vacuum drying to obtain the grape pip and grape skin extract. The production technology of the invention adopts multi-time extraction, multistage centrifugation and two-time desorption method of crude extract and fine extract, thus greatly improving the content of effective components.

The invention belongs to the field of natural organic chemistry, and relates to a purification method of rhizoma polygonati polysaccharides, in particular to a method for enriching and purifying the rhizoma polygonati polysaccharides by utilizing a water vapor reflux extraction and membrane separation technique and a macroporous resin coupling method. The method applies an enzymolysis technology to combine with the water vapor reflux extraction and membrane separation technique and the macroporous resin coupling method for separating and purifying the rhizoma polygonati polysaccharides, so that the rhizoma polygonati polysaccharides is completely extracted without pollution, a separation effect is good, the purity is high, the macroporous resin adsorptive selectivity is favorable, the adsorption is fast, the desorption is also fast, the adsorption capacity is larger, a separation effect is remarkable, the purification purity is high, a rhizoma polygonati polysaccharides semi-finished product with the content being 70 percent or more and a rhizoma polygonati polysaccharides finished product with the content being 95 percent or more can be obtained, and the defects of relatively lower conventional extraction rate and low extraction purity are overcome.

The invention discloses an extracting and purifying method of bee glue, which comprises the following steps: treating rough glue at -20-5 deg. c; bruising to 14-18 order at low temperature; sieving visual attribute with high effective metallic picker; dissolving and allocating bee glue with 60-95% (V / V) edible alcohol with 1 : 4-25; extracting 60-120 min with supersonic wave; stewing at -5 deg. c naturally; abstracting supernatant fluid; filtering; stripping heavy metal with resin column; vacuum-condensing; brewing and ripening with oak container. This invention also relates to a private extracting device.

The invention relates to a method for separating and extracting cepharanthine, which comprises the following steps of: taking a dry stephania tuberous root, crushing, adding 2 to 3L of water and 0.5 to 5ml of mixed bio-enzyme liquid into each kilogram of crushed material, soaking for 1 hour, adding 9 to 11L of 1 to 2 mass percent aqueous solution of HCL, heating to 90DEG C, and soaking for 3 hours; and separating out a liquid medicine, adding 9 to 11L of 1 to 2 percent aqueous solution of HCL into dregs, decocting for 3 hours, filtering, mixing filtrate, adsorbing by adopting a cation exchange column or cation cellulose column, enriching the cepharanthine, eluting by using solution that sodium hydroxide is added into 60 to 90 percent aqueous solution of ethanol until the saturation state as eluent, collecting eluent of a cepharanthine section, regulating the pH value to be 7, concentrating for recovering ethanol, filtering, dissolving a precipitate by using absolute ethanol, eluting and purifying by using an anion column, collecting eluent of a cepharanthine section, concentrating, crystallizing, and drying to obtain the cepharanthine. The method has the advantages of simple operation, light pollution, low cost, high yield, high purity of cepharanthine, and suitability for industrial production.

The invention discloses a traditional Chinese medicine for treating bacterial diseases in poultry, which is prepared from the following raw materials in parts by weight: 10-15 parts of wild chrysanthemum flower, 5-10 parts of Chinese goldthread, 8-10 parts of Scutellaria baicalensis, 8-10 parts of golden cypress, 10-15 parts of Chinese thorowax, 10-12 parts of Cape jasmine, 20-25 parts of Fagopyrum cymosum root, 25-30 parts of common andrographis herb, 8-10 parts of burdock, 25-30 parts of Chinese pulsatilla root and 30-35 parts of folium artemisiae argyi. The traditional Chinese medicine for treating the bacterial diseases in the poultry, disclosed by the invention, has the effects of preventing and treating the bacterial diseases caused by colibacillosis, salmonellosis, necrotizing enterocolitis, enterovirus syndromes, cholera fowls and the like in the poultry.

The invention relates to a composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves, which is characterized in that forsythin in forsythia suspense leaves is used as a nonpolar compound, and is easily dissolved in organic solvents such as methanol and ethanol and is difficultly dissolved in water, forsythiaside A is a polar compound, and is easily dissolved in water, methanol, ethanol and the like, and rutin is easily dissolved in methanol and hot ethanol and difficultly dissolved in water, through ethanol extraction, macroporous absorption resin separation and anti-phase silica gel column chromatography continuous extraction technology, forsythin, forsythiaside A and rutin can be performed with composite extraction in forsythia suspense leaves, the effective active components of forsythia suspense leaves can be completely extracted, and the resource of forsythia suspense leaves can be used in a reasonable mode; The method uses adsorption and desorption of macroporous adsorption resin to separate forsythin, forsythiaside A and rutin, forsythiaside A can be purified by using anti-phase silica gel and completely separated, the purity is high, the technology condition is simple, and the method provided by the invention is suitable for industrial production.

The invention discloses a traditional Chinese medicine for preventing and controlling respiratory diseases of animals, which is prepared from the following components in percentage by weight: 9 to 17 percent of liquoric root, 6 to 14 percent of gypsum, 4 to 12 percent of root of large-flowered skull, 3 to 11 percent of honeysuckle, 3 to 11 percent of sweeping forsythia, 2 to 10 percent of root bark of white mulberry, 2 to 10 percent of dried tangerine, 2 to 10 percent of tangerine seed, 2 to 10 percent of hawthorn, 2 to 10 percent of loguat leaf, 2 to 10 percent of root of straight ladybell, 3 to 7 percent of whiteflower hogfennel root, 3 to 7 percent of semen armeniacae amarae, 3 to 7 percent of rhizoma anemarrhenae, 1 to 3 percent of bryozoatum and 1 to 3 percent of borneol. By adopting the formula, from the screening of raw materials to the extraction and proportioning of active ingredients, a slow release / controlled release and targeting drug delivering technology is applied, so the medicine can effectively and quickly reach lesion sites, has the advantages of quick response, short treatment time, small side effect, convenient use, strong pertinence and high protectiveness, maintains the blood concentration in an effective treatment range, and effectively prevents and controls non typical Newcastle disease, bird flu, infectious laryngotracheitis, infectious bronchitis, colibacillus, mycoplasmosis and unexplained animal diseases such as head and face swelling and expiratory dyspnea.

The invention discloses healthcare food capable of improving cardiovascular and cerebrovascular circulation systems, increasing the release of NO in vascular endothelial cells and enhancing the anoxia resistance of the organism, a preparation method thereof and application of the healthcare food in preventing cardiovascular and cerebrovascular diseases and improving the anoxia resistance of the organism. The healthcare food disclosed by the invention is prepared from the following raw materials in parts by weight: 1-6 parts of American ginseng, 8-16 parts of radix salviae miltiorrhizae and 1-5 parts of panax pseudoginseng. The preparation method of the healthcare food disclosed by the invention completely extracts the active ingredients of radix salviae miltiorrhizae; and meanwhile, by adopting a micronization technology for the panax pseudoginseng and radix salviae miltiorrhizae, the surface area of the healthcare food is increased, the absorbance is improved, and the damage to the active ingredients of the panax pseudoginseng and radix salviae miltiorrhizae in the extraction process is reduced so as to improve the healthcare effect.

The invention provides a method for circularly extracting protein and humus from residual sludge after dehydration by combining an ultrasonic method with an acid process and relates to a method for extracting the protein and humus from the residual sludge. The method disclosed by the invention can be used for solving the technical problems that the existing sludge recycling method only can extract single substance from the sludge, maximum use of resource can not be realized and biodegradability is poor. The method disclosed by the invention comprises the following steps: firstly, ultrasonically cracking the dehydrated residual sludge which is produced by a municipal sewage treatment plant under the acidic condition; secondly, standing and settling for promoting precipitation of protein; thirdly, centrifuging and extracting the protein; fourthly, filtering and separating the humus, and recycling filtrate. The method has the advantages that the protein and humus can be extracted at the same time, extraction is complete, biodegradability of the sludge left after extraction is increased to 75.7% from 40.2%, and the method can be applied to the field of resource recycling treatment of municipal sludge.

An apparatus for extracting the contents from a refill pouch comprises a holding member for holding the pouch with its extraction port facing downward, and a pump attached to the extraction port of the pouch. The pump has a pump body defining a housing space having an inlet opening and an outlet opening, an inlet-side valve which communicates with the extraction port to open and close the inlet opening so that the contents can be introduced into the housing space from the pouch, and an outlet-side valve to open and close the outlet opening so that the contents in the housing space can be discharged through a nozzle that communicates with the outlet-side valve. The pump wall portion is constituted by a resiliently compressible, restorable and flexible material so that the contents in the housing space can be extracted through the nozzle by manually squeezing the pump wall portion thereby pressurizing the housing space.