The invention discloses an HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar, belonging to the technical field of food sugar detection. The method comprises the following steps: firstly, juicing and homogenizing grapes, ultrasonic extracting the juice, and then, centrifuging the juice, taking the supernatant, filtering the supernatant and using the filtrate as a test sample; respectively preparing standard working solutions with a series of concentrations of glucose, fructose and sucrose; the chromatographic conditions are as follows: a polystyrene-divinyl benzene bonded amino sugar column serves as a chromatographic column, the mobile phase is a mixture of acetonitrile and water with volume ratio of 70-80:30-20, and the flow velocity is 1mL/min, the column temperature is 30-35 DEG C, the detector is ELSD, the temperature of a detecting pool is 40-80 DEG C, and the sample size is 10 microliters; and then inspecting linear dependence and precision, and detecting stability and sample recovery. The method provided by the invention is simple and convenient to operate, small in time consumption, good in measurement result repeatability, high in precision and capable of accurately and quickly measuring the contents of glucose, fructose and sucrose in grape berry.