New method for extracting rhizoma polygonati polysaccharides from rhizoma polygonati

A Polygonatum Polysaccharide and Rhizoma Polygonatum technology, applied in the field of natural organic chemistry, can solve the problems of low extraction purity and low extraction rate, and achieve the effect of high extraction purity, large adsorption capacity and obvious separation effect

Inactive Publication Date: 2018-04-06
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying Polygonatum polysaccharides in Polygonatum Polygonatum by utilizing water vapor reflux extraction and membrane separation technology and macroporous adsorption resin coupling method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of method that utilizes water vapor reflux extraction and membrane separation technology and macroporous adsorption resin coupling method to enrich and purify Polygonatum Polysaccharide in Polygonatum, its steps are as follows:

[0020] (1) Grinding dry Polygonatum, crossing a 40-mesh sieve, adding purified water with a volume 8 times that of the crushed volume of Polygonatum, adding 0.5% cellulase, enzymolysis at 40°C for 1.5h, and then carrying out steam reflux extraction to extract 3 times, the extraction time is 2 hours;

[0021] (2) merging the Polygonatum extract after the treatment in (1), through microfiltration, the aperture range is 0.1 micron, and the pressure is controlled at 0.6MPa to obtain the filtrate;

[0022] (3) The filtrate through microfiltration is then subjected to ultrafiltration, the ultrafiltration molecular weight cut-off is 3,000 to 300,000, the temperature is controlled at 40°C, and the pressure is controlled at 0.4MPa t...

Embodiment example 2

[0028] Implementation Case 2: A method for enriching and purifying Polygonatum Polysaccharides in Polygonatum Polygonatum by using steam reflux extraction and membrane separation technology and macroporous adsorption resin coupling method, the steps are as follows:

[0029] (1) Grind the dried Polygonatum, pass through a 60-mesh sieve, add purified water whose volume is 10 times the volume of the crushed Polygonatum, add 0.8% cellulase, enzymatically hydrolyze at 60°C for 1 hour, then carry out water vapor reflux extraction, and extract twice , the extraction time is 3 hours;

[0030] (2) merging the Polygonatum extract after the treatment in (1), through microfiltration, the aperture range is 0.5 micron, and the pressure is controlled at 0.3MPa to obtain the filtrate;

[0031] (3) The filtrate through microfiltration is then subjected to ultrafiltration, the ultrafiltration molecular weight cut-off is 3,000 to 300,000, the temperature is controlled at 20°C, and the pressure i...

Embodiment example 3

[0037] Implementation Case 3: A method for enriching and purifying Polygonatum Polysaccharides in Polygonatum Polygonatum by using steam reflux extraction and membrane separation technology and macroporous adsorption resin coupling method, the steps are as follows:

[0038] (1) Grinding the dry sealwort, crossing 80 mesh sieves, adding purified water whose volume is 10 times that of the pulverized volume of sealwort, adding 1% cellulase, enzymolysis at 50°C for 1.5h, and then carrying out water vapor reflux extraction, extracting 3 times, the extraction time is 3 hours;

[0039](2) merging the Polygonatum extract after the treatment in (1), through microfiltration, the aperture range is 0.3 micron, and the pressure is controlled at 0.5MPa to obtain the filtrate;

[0040] (3) The filtrate through microfiltration is then subjected to ultrafiltration, the ultrafiltration molecular weight cut-off is 3,000 to 300,000, the temperature is controlled at 30° C., and the pressure is con...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying Polygonatum Polysaccharides, in particular to a method for enriching and purifying Polygonatum Polysaccharides in Polygonatum Polysaccharides by utilizing water vapor reflux extraction and membrane separation technology and macroporous adsorption resin coupling method. The present invention uses enzymatic hydrolysis technology combined with water vapor reflux extraction and membrane separation technology and macroporous adsorption resin coupling method to separate and purify Polygonatum polysaccharides, completely extracts Polygonatum polysaccharides, has no pollution, good separation effect, high purity, and good adsorption selectivity of macroporous resins , the adsorption is fast and the resolution is fast, the adsorption capacity is large, the separation effect is obvious, the extraction purity is high, and the semi-finished product of Polygonatum polysaccharide with a content of more than 70% and the final product of Polygonatum polysaccharide with a content of more than 95% can be obtained, which overcomes the relatively low extraction rate of conventional methods. The disadvantage of low extraction purity.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for purifying Polygonatum Polysaccharides, in particular to a method for enriching and purifying Polygonatum Polysaccharides in Polygonatum Polysaccharides by utilizing water vapor reflux extraction and membrane separation technology and macroporous adsorption resin coupling method. Background technique: [0002] Polygonatum sibiricum (scientific name: Polygonatum sibiricum), perennial herb. Also known as: Chicken Head Polygonatum, Yellow Chicken Vegetable, Pen Tube Vegetable, Claw Ginseng, Tiger Ginger, Chicken Claw Ginseng. It is a plant of the genus Polygonatum, the rhizome is horizontal, cylindrical, and the nodules are enlarged. Leaves whorl, sessile. There are several stem scars, the stem scars are relatively thick, the diameter of the thickest part can reach 2.5cm, and a few fibrous roots grow. Stem erect, cylindrical, single, 50-80cm high, smooth and gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 万莉姚德坤李玉伟苏学辉王雷
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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