Composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves

A technology of forsythiaside and compound extraction is applied in the field of forsythiaside and rutin to continuously extract the active ingredient forsythiaside A, which can solve the problems of complicated operation, low yield, and unreasonable utilization of forsythia resources, etc. To achieve the effect of simple process conditions and high purity

Active Publication Date: 2013-05-15
北京金翘生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in various literatures, only one or two active ingredients are extracted from Forsythia fruit or Forsythia leaves, which does not make rational use of Forsythia resources, resulting in a serious waste of resources
For example, Chinese patents 200610148179.0, 200810176773.X, 201010235729.9, etc. all conduct process research on an active ingredient. Chinese patent 201110209731.3 discloses a method for extracting forsythin and forsythiaside A from forsythia leaves. Water extraction, precipitation separation, organic solvent extraction, ethanol recrystallization, etc. to prepare forsythin and forsythiaside A, but it takes too long, the yield is relatively low, and a large amount of rutin is discarded
The method adopted in the prior art is directly water extraction or organic solvent extraction after water extraction, or alkaline water extraction, or acid water extraction, without comprehensively considering the activity of other active substances, and the operation is complicated and the cost is high
At present, there is no report on the simultaneous extraction and separation of forsythin, forsythiaside A and rutin from forsythia leaves

Method used

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  • Composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves
  • Composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves
  • Composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Taking 40 g of forsythia leaves as an example, the method for continuously extracting forsythin, forsythiaside A and rutin from forsythia leaves consists of the following steps:

[0025] (1) extract

[0026] Crush 40g of Forsythia leaves to 50 mesh, extract with 1000mL of ethanol with a volume concentration of 55% at 70°C for 50 minutes, the ratio of solid to liquid between Forsythia leaves and ethanol is 1:25g / mL, filter, and add 800mL of volume concentration to the filter residue Extract with 55% ethanol at 70°C for 50 minutes, combine the two extracts, let stand for 12 hours, filter, concentrate under reduced pressure at 55°C to recover ethanol, and obtain 665mL of concentrated solution, add 5mL of anhydrous Amount of ethanol Add 33.25mL of absolute ethanol to the concentrated solution, let it stand at room temperature for 12 hours, and filter to obtain the forsythia leaf extract.

[0027] (2) Resin separation

[0028] Adsorb the forsythia leaf extract through 72g ...

Embodiment 2

[0034] Taking 40 g of forsythia leaves as an example, the method for continuously extracting forsythin, forsythiaside A and rutin from forsythia leaves consists of the following steps:

[0035] (1) extract

[0036] Grind 40g of forsythia leaves to 50 mesh, extract with 1200mL of ethanol with a volume concentration of 55% at 70°C for 50 minutes, the ratio of solid to liquid of forsythia leaves and ethanol is 1:30g / mL, filter, and add 800mL of volume concentration to the filter residue Extract with 55% ethanol at 70°C for 50 minutes, combine the two extracts, let stand for 12 hours, filter, concentrate under reduced pressure at 55°C to recover ethanol, and obtain a concentrated solution, add 5mL of absolute ethanol to every 100mL of concentrated solution Add absolute ethanol to the concentrated solution, let stand at room temperature for 12 hours, and filter to obtain the forsythia leaf extract.

[0037] (2) Resin separation

[0038] Adsorb the forsythia leaf extract through a...

Embodiment 3

[0044] Taking 40 g of forsythia leaves as an example, the method for continuously extracting forsythin, forsythiaside A and rutin from forsythia leaves consists of the following steps:

[0045] (1) extract

[0046] Crush 40g of Forsythia leaves to 50 mesh, extract with 1600mL of ethanol with a volume concentration of 55% at 70°C for 50 minutes, the ratio of solid to liquid between Forsythia leaves and ethanol is 1:40g / mL, filter, and add 600mL of volume concentration to the filter residue Extract with 55% ethanol at 70°C for 50 minutes, combine the two extracts, let stand for 12 hours, filter, concentrate under reduced pressure at 55°C to recover ethanol, and obtain a concentrated solution, add 5mL of absolute ethanol to every 100mL of concentrated solution Add absolute ethanol to the concentrated solution, let stand at room temperature for 12 hours, and filter to obtain the forsythia leaf extract.

[0047] (2) Resin separation

[0048] Adsorb the forsythia leaf extract thro...

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Abstract

The invention relates to a composite extracting method for forsythin, forsythiaside A and rutin in forsythia suspense leaves, which is characterized in that forsythin in forsythia suspense leaves is used as a nonpolar compound, and is easily dissolved in organic solvents such as methanol and ethanol and is difficultly dissolved in water, forsythiaside A is a polar compound, and is easily dissolved in water, methanol, ethanol and the like, and rutin is easily dissolved in methanol and hot ethanol and difficultly dissolved in water, through ethanol extraction, macroporous absorption resin separation and anti-phase silica gel column chromatography continuous extraction technology, forsythin, forsythiaside A and rutin can be performed with composite extraction in forsythia suspense leaves, the effective active components of forsythia suspense leaves can be completely extracted, and the resource of forsythia suspense leaves can be used in a reasonable mode; The method uses adsorption and desorption of macroporous adsorption resin to separate forsythin, forsythiaside A and rutin, forsythiaside A can be purified by using anti-phase silica gel and completely separated, the purity is high, the technology condition is simple, and the method provided by the invention is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of forsythia leaf separation, in particular to a method for continuously extracting active ingredients forsythiaside A, forsythin and rutin from natural plant forsythia leaves. Background technique [0002] Forsythia is a deciduous shrub belonging to the Oleaceae family Forsythia genus. It is a commonly used medicinal material. The fruit is traditionally used as medicine. However, folk surveys show that Forsythia leaves can relieve constipation, prevent colds, lower blood lipids, and lower blood pressure. Domestic researchers have conducted a preliminary analysis of the chemical components of Forsythia leaves, and used Forsythia leaves to make tea as a nourishing conditioning drink. Forsythia leaves are often used as health-care tea. Chemical studies have proved that the chemical composition of Forsythia leaves and the commonly used medicinal material Forsythia fruit has good consistency, mainly containing l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H15/18C07H17/07C07H1/08
Inventor 杨建雄李忠岐刘伟霞颜礼有
Owner 北京金翘生物科技有限公司
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