Rhodococcus ruber for generating natural haematochrome and preparation method and application thereof
A technology of natural red pigment and Rhodococcus erythropoiesis, which is applied in the field of fermentative production of Rhodococcus erythropoiesis strains, and can solve the problems such as being unable to meet the needs of rapid industrial development.
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Embodiment 1
[0044] Rhodococcus, whose taxonomy is Rhodococcus ( Rhodococcus ruber ), the deposit number is CGMCC No. 12604.
[0045] The filtering method is as follows:
[0046] (1) Take 5 grams of soil sample (rich in humus) and put it in the seed medium, the composition of the seed medium (g / L): glucose 10g, peptone 2g, yeast powder 5g, sodium chloride 10g, K 2 HPO 4 2g, pH 7.0~7.2; shake culture at 150~180rpm, 30~40℃, 36~48h to enrich microorganisms.
[0047] (2) Use an inoculation loop to aseptically pick the enriched microbial liquid from (1), and use the composition of the seed medium (g / L): glucose 10g, peptone 2g, yeast powder 5g, sodium chloride 10g, K2 HPO 4 2g, pH 7.0~7.2, mark on the plate, put it in the incubator at 30~40℃, culture for 36~48h;
[0048] (3) Observe the red colony growing on the plate in (2), and aseptically pick it into the seed medium composition (g / L): glucose 10g, peptone 2g, yeast powder 5g, sodium chloride 10g, K 2 HPO 4 2~6g, pH 7.0~7.2, separa...
Embodiment 2
[0053] Rhodococcus erythrococcus ( Rhodococcus ruber ), the preservation number is CGMCC No. 12604. The method for preparing natural red pigment:
[0054] (1) Seed culture and medium
[0055] The fermentation strain used was CGMCC No. 12604, and the composition of the seed medium (g / L): glucose 20g, peptone 4g, yeast powder 5g, sodium chloride 10g, K 2 HPO 4 3g, pH 7.0~7.2; 170 rev / min shaker culture, 34°C, 45h.
[0056] (2) The fermentation strain used is CGMCC No. 12604, and the composition of the fermentation medium is (g / L): 20-40 g of carbohydrate material, 4 g of peptone, 15 g of yeast powder, 15 g of corn steep liquor, 10 g of sodium chloride, Sodium citrate 2.5, MgSO4 7H 2 0 0.15g, K 2 HPO 4 3g, pH 7.0-7.2.
[0057] (3) Fermentation culture: Insert the cultured seeds into the fermentation medium at an inoculum amount of 1%~10% (v / v), culture temperature is 35°C, and fermentation ventilation volume is 8m 3 / h, tank pressure 0.05 MPa, stirring speed 120~150 rpm...
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