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Rhodococcus ruber for generating natural haematochrome and preparation method and application thereof
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A technology of natural red pigment and Rhodococcus erythropoiesis, which is applied in the field of fermentative production of Rhodococcus erythropoiesis strains, and can solve the problems such as being unable to meet the needs of rapid industrial development.
Active Publication Date: 2017-02-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Animal and plant-derived pigments can no longer meet the needs of rapid industrial development
Method used
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[0046] (1) Take 5 grams of soil sample (rich in humus) and put it in the seed medium, the composition of the seed medium (g / L): glucose 10g, peptone 2g, yeastpowder 5g, sodiumchloride 10g, K 2 HPO 4 2g, pH 7.0~7.2; shake culture at 150~180rpm, 30~40℃, 36~48h to enrich microorganisms.
[0047] (2) Use an inoculation loop to aseptically pick the enriched microbial liquid from (1), and use the composition of the seed medium (g / L): glucose 10g, peptone 2g, yeastpowder 5g, sodiumchloride 10g, K2 HPO 4 2g, pH 7.0~7.2, mark on the plate, put it in the incubator at 30~40℃, culture for 36~48h;
[0048] (3) Observe the red colony growing on the plate in (2), and aseptically pick it into the seed medium composition (g / L): glucose 10g, peptone 2g, yeastpowder 5g, sodiumchloride 10g, K 2 HPO 4 2~6g, pH 7.0~7.2, separa...
Embodiment 2
[0053] Rhodococcus erythrococcus ( Rhodococcus ruber ), the preservation number is CGMCC No. 12604. The method for preparing natural red pigment:
[0054] (1) Seed culture and medium
[0055] The fermentation strain used was CGMCC No. 12604, and the composition of the seed medium (g / L): glucose 20g, peptone 4g, yeast powder 5g, sodium chloride 10g, K 2 HPO 4 3g, pH 7.0~7.2; 170 rev / min shaker culture, 34°C, 45h.
[0056] (2) The fermentation strain used is CGMCC No. 12604, and the composition of the fermentation medium is (g / L): 20-40 g of carbohydrate material, 4 g of peptone, 15 g of yeast powder, 15 g of corn steep liquor, 10 g of sodium chloride, Sodium citrate 2.5, MgSO4 7H 2 0 0.15g, K 2 HPO 4 3g, pH 7.0-7.2.
[0057] (3) Fermentation culture: Insert the cultured seeds into the fermentation medium at an inoculum amount of 1%~10% (v / v), culture temperature is 35°C, and fermentation ventilation volume is 8m 3 / h, tank pressure 0.05 MPa, stirring speed 120~150 rpm...
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Abstract
The invention relates to rhodococcus ruber for generating natural haematochrome and a preparation method and application thereof, wherein the haematochrome is generated through microbial fermentation; the strain category belongs to rhodococcus ruber; the preservation numbr is CGMCC No.12604. The invention also relates to a preparation method of the fermentation natural haematochrome. The fermentation strain of rhodococcus ruber CGMCC No.12604 is used for inoculating the cultured seeds into a fermentation culture medium according to the inoculation quantity according to the inoculation quantity of 1 percent to 10 percent (v / v), wherein the culture temperature is 30 to 40 DEG C; the fermentation ventilation quantity is 5 to 13m<3> / h; the tank pressure is 0.02 to 0.09 MPa; the stirring speed is 120 to 150 r / min; the fermentation time is 120 to 144h; in the fermentation process, sterile air is firstly introduced; the pH of the fermentation liquid is maintained at 6.5 to 7.5 by ammoniumhydroxide or alkali liquor; according to the fermentation result, the yield of the haematochrome is 1 to 2g / L. The invention also discloses an extraction method of the rhodococcus ruber haematochrome.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a microorganism producing natural red pigmentRhodococcus rubrum and a method for preparing the red pigment. More specifically, it is a strain for fermenting and producing Rhodococcus red pigment. This strain is a new strain found in the present invention that can effectively produce red pigment. Fermentation and production of this strain is simple and stable. Background technique [0002] Pigment is widely used in food industry, cosmetics production, textile and daily necessities production, bringing good added value to products. However, the potential hazards of synthetic pigments, such as carcinogenicity and teratogenicity, are increasingly rejected, while natural pigments are increasingly favored by people. The sources of natural pigments are mainly divided into animal pigments, plant pigments, microbial pigments and mineral pigments. Animal and plant-derived pigments can no long...
Claims
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IPC IPC(8): C12N1/20C12P1/04C12R1/01
CPCC12P1/04C12N1/205C12R2001/01
Inventor 王德培于淼陈红爽
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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