The invention discloses application of VvERF27 in regulation and control of grape fruit
ripening, and belongs to the technical field of
biology. The invention discovers the effect of the VvERF27 in regulating and controlling the
ripening of the grape fruits for the first time. A
subcellular localization test shows that the VvERF27 is localized in a
cell nucleus; a GUS
staining test proves that the activity of the VvERF27
promoter is influenced by
hormone; by respectively constructing an overexpression vector and a silent vector, it is found that after the VvERF27
gene is overexpressed, the expression quantity of a grape fruit
ripening gene is increased, and the grape fruit ripening process is accelerated, and after the VvERF27
gene is silent, the expression quantity of the grape fruit ripening gene is reduced, and the grape fruit ripening process is delayed, that is, the VvERF27 gene plays a positive regulation role in the grape fruit ripening process. The method not only helps to improve the economic value of grapes, but also can promote the cultivation of early-maturing varieties of grapes, and is of great significance in the field of grape planting.