25 results about "Grape berry" patented technology

The invention relates to a KASP molecular marker primer combination for identifying rose fragrance traits of grape fruits and application of the KASP molecular marker primer combination in grape germplasm resource screening, variety identification and assistant breeding. Compared with a VvDXS 422G / T site marking method in the prior art, the primer combination (F1, F2, R) provided by the invention is higher in sensitivity and specificity. The primer combination (F1, F2, R) provided by the invention can effectively distinguish the rose fragrance variety with the GT or TT genotype from the GG genotype variety without the character, so that the high-throughput screening efficiency is remarkably improved, and the applicability is wide. The technology can be widely applied to early screening of grape varieties, germplasm resource evaluation and molecular marker-assisted breeding, is beneficial to improving the breeding efficiency of rose-fragrance grapes, and has important breeding value and industrial application prospect.

The present invention relates to a sorter roller (1) for a sorting table allowing the removal of foreign bodies that remain mixed with the products of the harvest of small fruits, more particularly small fruits such as grape berries after destemming the grape harvest, the sorting table including a series of sorter rollers (1) arranged so as to provide a plurality of screening openings through which the small fruits to be sorted can pass and fall, the sorter roller (1) having a series and an alternation of regularly spaced grooves (10) and ridges (11), each ridge (11) extending around the longitudinal axis (X) of the sorter roller (1) in a ridge plane perpendicular to the longitudinal axis (X) of the sorter roller (1), each groove (10) having a concave outer surface resulting from the rotation of the rotational profile of the groove (10) about the longitudinal axis (X) of the sorter roller (1) and connecting two adjacent ridges (11), namely a first ridge (11) and a second ridge (11). The invention is characterised in that the rotational profile of each groove (10) is asymmetrical. The invention also relates to a sorting table comprising a series of such sorter rollers.

This technology provides targeted genome editing techniques for altering the color of parts of grape plants and their fruits. Specifically, this technology relates to using genome editing methods to generate edits in target genes that cause large fragment inactivation deletions, thereby altering the color traits of plants and fruits. These target genes include genes and genetic elements associated with plant color and pigment deposition (e.g., the PDS1 gene, the mybA1 gene, and the Gret1 retrotransposon).

This invention belongs to the field of grape cultivation technology, specifically relating to a fruit enlargement agent for Daqing grapes, its preparation method, and its application. The fruit enlargement agent for Daqing grapes is prepared from an aqueous solution of gibberellin, thiabendazole, and brassinolide. Specifically, the concentration of gibberellin in the fruit enlargement agent is 20 mg / L~25 mg / L, the concentration of thiabendazole is 1.0 mg / L~1.25 mg / L, and the concentration of brassinolide is 0.025 μg / L~0.050 μg / L. The fruit enlargement agent of this invention, combined with fruit thinning treatment, can increase the weight of a single Daqing grape berry by at least 20% while retaining the soluble solids content of the grape.

The invention relates to a composition for making a tea beverage, the composition comprising tea which may be ground tea in combination with plant cell culture grown in vitro in a large scale. The plant cell culture grown in vitro in a large scale may be derived from any plant, such as a fruit including but not limited to grape berry, pomegranate, olive, and the like. The composition may be inserted into a pod and be used in a machine or inserted into a tea bag or a container.

According to an exemplary embodiment of the present disclosure, a cosmetic composition comprising a fermented grape extract obtained by fermenting grape (Vitis vinifera) berries using fungus of the genus Botrytis is disclosed.

The utility model discloses a grape fruit size grading device which comprises a conveying belt, a grading assembly used for conducting size grading on grape fruits is arranged at the tail end of the conveying belt, the grading assembly is arranged at the tail end of the conveying belt in a downward inclined mode, and the grading assembly comprises a blocking edge, a feeding plate, a discharging plate and a grading rod. The blocking edges are fixed to the two side edges of the conveying belt, a feeding plate and a discharging plate are fixed to the inner sides of the blocking edges, and a grading rod is fixed between the feeding plate and the discharging plate. According to the grape fruit grain size grading device, the feeding plate, the discharging plate and the grading rods which are obliquely arranged are arranged at the tail of the conveying belt, grape fruit grains conveyed on the conveying belt can freely slide downwards from the feeding plate, the discharging plate and the grading rods, and the grape fruit grains are arranged through the openings which are sequentially increased between the grading rods; grape fruit grains with different sizes can fall at different positions on the grading rod, so that the automatic grading work of the grape fruit grains can be realized.

The invention relates to a grape fruit volume whole genome selective breeding method. The method comprises the following steps: preparing data; the method comprises the following steps: collecting a sample of a grape germplasm resource, and carrying out fruit volume measurement on the sample; performing whole genome sequencing on the samples, and excluding unqualified samples with low quality and joint pollution in the samples to obtain high-quality second-generation sequencing data; and mapping the high-quality next-generation sequencing data to a high-quality reference genome PN40024 for variation typing, performing variation calling in GTX software, filtering and screening to obtain effective variation sites, and obtaining variation sites. The invention relates to the technical field of plant genetic breeding. According to the grape fruit volume whole genome selective breeding method, the limitation of a traditional breeding method in the aspect of increasing the grape fruit volume is overcome, wide application prospects and important popularization value are brought to the field of grape breeding, and sustainable development and progress of the grape industry are expected to be promoted.

The invention belongs to the technical field of fruits, vegetables and genes, and discloses application of a grape VvNAC17 gene in regulation and control of postharvest grape berry abscission and a carrier, the gene sequence of the VvNAC17 gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoding protein of the VvNAC17 gene is as shown in SEQ ID NO.2. The VvNAC17 silencing technology disclosed by the invention is accurately regulated and controlled from a gene molecule level, the uncertainty influence of an external environment and storage and transportation conditions is reduced, the fruit grain falling rate is remarkably reduced, and the commodity and economic benefits during the storage and transportation period of grapes are improved. By constructing a VvNAC17-RNAi vector, the invention verifies the key positive regulation effect of the VvNAC17 gene on postharvest fruit falling for the first time. The gene silencing technology does not depend on complex storage and transportation conditions, potential influences of chemical agents on fruit quality and the environment are avoided, and an innovative solution is provided for the modern grape storage and transportation technology.

The utility model relates to the technical field of grape processing and threshing, and discloses a grape processing and threshing device which comprises a base, a supporting frame is fixedly assembled on the top of the base, a classification assembly is arranged on the outer wall of the supporting frame, a spring assembly is fixedly assembled on the top of the supporting frame, and a threshing box is fixedly installed on the top of the spring assembly. The controller transmits a signal, and the vibration motor starts to work, so that the vibration motor can enable the threshing box to repeatedly shake under the action of the spring assembly, the threshing box can separate grapes from stems according to the vibration principle, meanwhile, damage to grape fruits can be effectively reduced, and after the grapes are separated from the stems, the grapes can be separated from the stems through the vibration motor. Qualified grape fruits enter the inner wall of the first discharging port through a filtering plate, and unqualified grape fruits fall on the top of a slope plate through filtering holes in the top of the filtering plate and enter the inner wall of a first grape storage box through a second discharging port to be collected, so that the device can classify the sizes of grapes during threshing.

The invention discloses a grape fruit grain segmentation method in a fruit thinning period based on combination of wavelet transform and a visual state space model, and belongs to the technical field of image processing. The method comprises the steps that a data set is constructed, preprocessed and enhanced; constructing a double-branch enhanced network; the Stem module receives an input image to complete feature preliminary extraction; the output characteristic flows through a three-stage WT-Mama stage module; generating a multi-dimensional feature map at each stage of WT-Mama stage, and fusing to obtain a global feature; training and optimizing the model; and performing real-time fruit grain segmentation reasoning. According to the method, the edge visibility in a complex illumination and shielding scene is improved, the characterization capability of a global structure of dense fruit grains is enhanced, the precision and robustness of fruit grain segmentation in the fruit thinning period and the field real-time reasoning efficiency are improved, and a key technical support is provided for grape phenotype analysis and intelligent fruit thinning decision.

This utility model relates to a vibrating sieve for selecting grape berries for winemaking, comprising: a destemming mechanism for separating grape berries and stems; a vibrating sieving mechanism for sieving to remove fine impurities and rotten grapes; a stem guiding mechanism including a hinged, swing-mounted, grid-like guide component; and a stem-removing mechanism including a hollow roller rotatably mounted below the guide component, the surface of which is equipped with a lever that is interleaved with the grid on the guide component; when the hollow roller drives the lever to rotate, it pushes the stems intercepted by the guide component upwards and transfers them to the stem conveyor belt. This utility model replaces the traditional multi-layer grading guide roller method of separating grape berries by adding a stem guiding mechanism and a stem-removing mechanism to remove stems, effectively solving problems such as incomplete grape separation and grapes easily getting stuck between the guide rollers and causing damage.

This invention provides strigolactone via VvORR9 The methods and applications of gene regulation of grape berry shape belong to the fields of plant molecular biology and agricultural biotechnology. This study involves regulating grape berry shape through exogenous application of the synthetic strigolactone analog GR24. VvORR9 Gene expression levels alter the longitudinal and transverse diameters of the fruit, as well as the fruit shape index. This invention, combining correlation analysis, stable transformation in grapes, and heterologous transformation in tomatoes, constructs a molecular regulatory network for strigolactone-mediated grape fruit shape regulation. It is the first to demonstrate that the strigolactone signaling pathway participates in grape fruit morphogenesis and identifies regulatory factors that respond to strigolactone. VvORR9 This provides a theoretical basis for the precise regulation of grape fruit shape and molecular breeding.

The invention discloses application of VvERF27 in regulation and control of grape fruit ripening, and belongs to the technical field of biology. The invention discovers the effect of the VvERF27 in regulating and controlling the ripening of the grape fruits for the first time. A subcellular localization test shows that the VvERF27 is localized in a cell nucleus; a GUS staining test proves that the activity of the VvERF27 promoter is influenced by hormone; by respectively constructing an overexpression vector and a silent vector, it is found that after the VvERF27 gene is overexpressed, the expression quantity of a grape fruit ripening gene is increased, and the grape fruit ripening process is accelerated, and after the VvERF27 gene is silent, the expression quantity of the grape fruit ripening gene is reduced, and the grape fruit ripening process is delayed, that is, the VvERF27 gene plays a positive regulation role in the grape fruit ripening process. The method not only helps to improve the economic value of grapes, but also can promote the cultivation of early-maturing varieties of grapes, and is of great significance in the field of grape planting.

This invention belongs to the fields of fruit and vegetable science and gene technology, and discloses the application and vector of the grape VvNAC17 gene in regulating postharvest grape berry abscission. The gene sequence of the VvNAC17 gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. The VvNAC17 silencing technology of this invention provides precise regulation at the gene molecular level, reducing the uncertainties of external environment and storage and transportation conditions, significantly reducing berry abscission rate, and improving the marketability and economic benefits of grapes during storage and transportation. By constructing a VvNAC17-RNAi vector, this invention verifies for the first time the key positive regulatory role of the VvNAC17 gene in postharvest berry abscission. The gene silencing technology does not rely on complex storage and transportation conditions and avoids the potential impact of chemical agents on fruit quality and the environment, providing an innovative solution for modern grape storage and transportation technology.

The present application relates to the technical field of wine brewing, in particular to a method for brewing Thompson Seedless white grape wine by carbon dioxide impregnation fermentation, comprising the following steps: adopting a process scheme combining carbon dioxide whole fruit impregnation and sequential inoculation of multiple yeasts, placing whole Thompson Seedless white grape berries in a closed carbon dioxide environment for impregnation at 28-32 DEG C for 8-12 days to promote the dissolution and conversion of skin aroma precursors; after crushing, juice extraction and clarification, first inoculating non-wine yeast such as Cryptococcus or Saccharomycodes deemii for fermentation for 48 hours, and then inoculating wine yeast QA23 or EC1118 to complete main fermentation and segmented temperature control. The present application significantly increases the content of ester and terpene aroma substances, enhances fruitiness and floral characteristics, effectively improves the complexity and level of aroma, guarantees fermentation stability, and provides a new technical approach for the quality upgrading of Thompson Seedless white grape wine.

Process for inducing thickening of the skin of grape berries in a vine cultivation, which involves inserting an active yeast for winemaking use with a water residue of at least 40% into a pressurization chamber, inside which a fluid is forced at a lysis pressure greater than 1000 bar to subject the active yeast to said lysis pressure with consequent breakage of the molecular structure of the yeast and obtaining an inactivated yeast or derivative thereof containing a free amino acids fraction extracted from the cytosol of the same yeast. The depressurization of the pressurization chamber then takes place and the inactivated yeast or derivative thereof is extracted from the pressurization chamber itself. Furthermore, the method involves dissolving the inactivated yeast or derivative thereof in a solvent in order to obtain a treatment solution, distributing the treatment solution on at least one vine plant of the vine cultivation and allowing the vine plant to absorb the free amino acids fraction extracted from the cytosol.

The invention discloses a grape fruit longitudinal diameter whole genome selective breeding method. The method comprises the following steps: acquiring variation site data and phenotype data of 323 grape samples; 323 samples are divided into a training set and a test set, the training set comprises 259 samples, and the test set comprises 64 samples; performing whole-genome association analysis on the training set samples to find variation points significantly associated with the longitudinal diameter of the fruit; importing the associated variation points and phenotype data into five classic regression models, carrying out model training for predicting the size of the longitudinal diameter of the grape, and screening out an optimal prediction model; and importing a variation site data set of 64 test sets into the screened optimal prediction model for prediction, and evaluating the accuracy of the model. The invention relates to the technical field of breeding screening. On the basis of 5000 variation sites and a Ridge regression model, in 64 test samples, the linear correlation coefficient of a model predicted value and an actual phenotype value reaches R = 0.96, which indicates that the model can replace the level of manual detection.

This application discloses a grape segmentation method based on multi-source feature fusion, belonging to the field of image processing technology. It establishes an instance segmentation network, MFFNet, based on multi-source feature fusion. MFFNet collaboratively utilizes powerful semantic features extracted by the Swing Transformer and explicit boundary information generated by parallel edge branches. ASFF adaptively fuses multi-scale information for grape segmentation, employing a staged feature fusion strategy to perform targeted feature fusion at different stages, improving the accuracy and robustness of grape segmentation in complex scenes. MFFNet effectively solves the segmentation challenges of dense grape berries, occlusion, and blurred boundaries in complex field environments. The staged feature fusion strategy enhances the model's ability to perceive and depict object contours, effectively addressing the problems of instance adhesion and unclear boundaries.

The invention relates to the technical field of grape fruit transverse-diameter whole-genome selective breeding, and particularly discloses a grape fruit transverse-diameter whole-genome selective breeding method which comprises the following steps: performing whole-genome sequencing on a plurality of samples by utilizing a Novaseq plaatform sequencing technology; performing quality control on the original data by using fastp software, eliminating low-quality reads and joint pollution in a sample, and obtaining high-quality re-sequencing data; mapping the re-sequencing data to a generic genome Grapcan v.1.0 constructed by taking a complete PNT2T genome as a skeleton through a giraffe command in vg software, and carrying out variation typing, so as to obtain a bam file; and processing the bam file by using GTX software to obtain a gvcf file, and integrating the variation data set through a joint program of GTX. According to the transverse-diameter whole-genome selective breeding method for the grape fruits, a whole-genome selective breeding strategy based on machine learning is used, and the transverse-diameter character phenotype of the grape fruits is successfully predicted.

The invention discloses a monocular vision-based grape fruit diameter and particle weight measurement model construction and detection method. The method comprises the following steps of: obtaining a visual data set of a measured grape; carrying out noise reduction, filtering and segmentation processing on the obtained grape picture; dividing the data set into a training set, a verification set and a prediction set; constructing a network fusion depth model according to the data set; constructing a loss function for training the model; establishing a visual feature extraction mechanism of the three-dimensional fruit diameter and the weight of the detected grapes, and inputting the training set and the verification set into the network fusion depth model for training; the prediction set is used for evaluating the precision of the deep network model, the trained network is fused with the deep model to predict the fruit diameter and weight result of the corresponding single grape, and the fruit diameter and weight of each grape can be rapidly and nondestructively detected. According to the method, the fruit diameter and the weight of each grape can be rapidly and nondestructively obtained, the labor cost and the time cost for measuring the grapes one by one in the season of the grapes are greatly reduced, and finally accurate sorting of the grape quality is achieved.

A process for producing a grape-based food preparation for beverages, comprising the steps of preparing a plurality of whole grape berries; actively removing the respective seeds from each of said grape berries; drying said grape berries to obtain a 100% seedless grape preparation; and packaging said 100% seedless grape preparation. The invention also extends to the preparation thus obtained and the beverage derived therefrom.

The invention belongs to the technical field of grape management, and particularly relates to an open-air grape grain number non-destructive calculation method, which comprises the following steps: S1, photographing grapes in a fruit setting period or a harvesting period; s2, performing target detection on the grape berries based on a deep learning model to obtain the range of grape fruits; s3, based on the result of S2, obtaining a plurality of parameters of the grapes, including morphological characteristic parameters, large and small grains and abnormal grains; and S4, establishing a relationship between the parameters obtained in the S3 and an actual grape number, and applying a result to other grape bunch image counting. The invention further provides a system for non-destructive calculation of the number of open-air grapes. According to the method, multi-feature fusion counting is realized, the relationship between a plurality of morphological features and the number of grape fruits is adopted, and the method is stable and reliable.

The application discloses a reagent, a method and application for reducing the astringency of grape fruits, wherein the reagent comprises strigolactone, brassinolide and melatonin. It is found that strigolactone can significantly reduce the astringency of grape fruits at a concentration of 1-3 μmol / L, with a maximum reduction of 50.59%, and does not affect the coloring of the fruits; low-concentration brassinolide (0.1-0.6 mg / L) can reduce the astringency while increasing the contents of total phenols and anthocyanins, and synergistically promote the coloring; and melatonin has astringency-reducing effect at a concentration of 5-50 μmol / L. Transcriptome analysis shows that strigolactone blocks tannin synthesis by inhibiting the expression of DFR and CCR genes, and brassinolide remodels the metabolic flow to the anthocyanin branch through BZR1. The application provides an efficient and safe technical means for the precise improvement of the flavor quality of table grapes.