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The application discloses a plant composition with soothing effect and a preparation method and application thereof, and the composition comprises saponins extracted from radix cynanchi rossii and radix anemarrhenae, and polysaccharides extracted from the radix anemarrhenae, and the mass ratio of the saponins to the polysaccharides is (1-5):(1-3). The technical scheme of the application is as follows: the radix cynanchi rossii and the radix anemarrhenae are mixed in a certain proportion, crushed, extracted by an alcohol solution, and then saponins are obtained through composite membrane separation; the remaining residues are extracted by water, impurities are removed by a clarifying agent, and the radix anemarrhena polysaccharides are obtained through ultrafiltration; and the saponins and the polysaccharides are compounded in a certain proportion, supplemented by polyhydric alcohol to be dissolved, so that the composition with the soothing effect is obtained, and the composition can be applied to the preparation of skin care cosmetics, especially soothing and anti-irritation products.