The patent discloses a method for quickly establishing a Cabernet Sauvignon grape pericarp
callus regeneration
system, and belongs to the technical field of
cell engineering. The method takes the pericarp of Cabernet Sauvignon grape as an explant, realizes efficient induction and rapid regeneration of
callus through three key steps of explant disinfection treatment, primary
callus induction culture, callus proliferation and subculture. The method optimizes the culture medium formula, adds specific concentrations of 6-BA, KT, IAA, NAA and 2,4-D in the induction medium, and significantly improves the induction rate and growth rate of callus. Experimental results show that the stable callus regeneration
system can be obtained in 40-50 days by using the method, the callus induction rate can reach more than 90%, and the
survival rate of regenerated plants is high. The patent has the advantages of simple operation, good
repeatability and
short cycle, and provides an effective technical means for genetic improvement,
gene function research and rapid propagation of
wine grapes, and has important theoretical value and application prospect.