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Tissue culture and rapid propagation method for wine grape variety

A technology for tissue culture and rapid propagation of grapes, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to take root, lodging of sterile seedlings, poor growth potential, etc., and is beneficial to transplanting and widely used. Effect

Inactive Publication Date: 2012-07-11
GANSU AGRI UNIV
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AI Technical Summary

Problems solved by technology

If the exogenous hormones added to the basic medium are the same, some varieties will take root and grow better, while some varieties will not take root and grow poorly.
Coagulant (support agent) - agar also has a great influence. If the concentration of agar is too high, it will hinder the elongation and growth of roots; if the concentration of agar is too low, there will be disadvantages such as lodging of sterile seedlings factor

Method used

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Embodiment Construction

[0017] The present invention is described in further detail below in conjunction with embodiment.

[0018] A method for tissue culture and rapid propagation of wine grape varieties is completed according to the following steps:

[0019] Media selection:

[0020] (Take 1L medium as an example): GS+sucrose (20g / L) + agar (4.0g / L or 3.5g / L)+0.002g / L penicillin sodium, pH=5.8;

[0021] The specific production method of the culture medium:

[0022] (Taking 1L culture medium as an example): 1) Add 300-350ml of distilled water to a 500ml beaker; 2) Take each mother liquor of GS into the beaker in step 1 according to the proportion; set aside; 3) Add 400- 500ml of distilled water is heated until boiling; 4) agar and 0.002g / L penicillin sodium for injection are successively added to the boiling distilled water described in 3), boiled 3 times, fully stirred and dissolved; for later use; 5) the 2 ) into the boiling solution obtained in 4), stir well, then add sucrose, and stir well ag...

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Abstract

The invention relates to a tissue culture and rapid propagation method for a wine grape variety, which comprises the following steps of: (1) culture medium selecting and making method: selecting GS as a basic culture medium, and adding cane sugar as a sugar source, agar as a coagulating agent and antibiotics as penicillin sodium for injection, wherein the pH value is 5.5-6.0; (2) material selecting and inoculating method: selecting a aseptically cultured aseptic adult grape seedling, scissoring the aseptic seedling stem under aseptic conditions, and inoculating the aseptic seedling stem on the culture medium made in the step (1); and (3) culture environment: controlling the illumination intensity at 4000-6000 LX, and controlling the temperature at 23-25 DEG C. The invention has the characteristic that the obtained wine grape tissue culture seedling is widely popularized and applied to the rapid propagation and planting of wine grape and has the characteristics of: (1) rapid propagation: the propagation period of wine grape can be shortened; and (2) strong root forming ability: the obtained aseptic seedling has developed root system, which is beneficial to transplantation.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of wine grape varieties, in particular to a method for tissue culture and rapid propagation of seven wine grape varieties including Pinot Noir, Cabernet Sauvignon, Betta, Vitis vinifera, LDP, L33 and SO4. Background technique [0002] The rapid propagation technology of grapes began in the 1940s, mainly including cutting propagation, grafting propagation, seed propagation, tissue culture and so on. Among them, tissue culture is widely used in the rapid propagation of wine grapes. Its advantages can not only speed up the propagation speed, but also combine heat treatment with shoot tip culture to effectively eliminate viruses and obtain non-toxic seedlings. The common wine grape varieties in my country include Pinot Noir, Cabernet Sauvignon, Betta, Vinegrass, LDP, L33, SO4 and so on. In the actual production process, in order to improve the economic benefits of wine grapes, meas...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 栗孟飞李唯杨德龙孙萍姜寒玉张玲瑜李甜甜周兰兰
Owner GANSU AGRI UNIV
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