Cuttage rooting method for mandarin orange

Abstract

The cutting propagation rooting method sequentially comprises the steps of selecting cuttings, pruning cuttings, performing hormone treatment and performing cutting, so that the kumquat has a relatively high rooting rate and a relatively large number of roots, the propagation period is shortened, the labor force is saved, the production cost is reduced, the method is simple and easy to implement, meanwhile, the land utilization rate is increased, and the economic benefit is increased. The planting survival rate is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cuttings of kumquat, and in particular to a method for rooting kumquat cuttings. Background Art

[0002] Mountain kumquat, also known as mountain kumquat and mountain golden bean, is an evergreen fruit tree of the genus Kumquat in the Rutaceae family. It is a unique germplasm resource in my country. It is divided into two types: monoembryonic and polyembryonic. The plant is short and can bloom many times a year. The fruit is as big as a bean when ripe, and the peel is golden. It can be used as a potted plant and is an excellent ornamental tree species. Its roots, leaves, and fruits can be used as medicines, and can also be used as citrus rootstocks and breeding materials. Mountain kumquat has the characteristics of short childhood, dwarfing, and complete citrus fruit structure. It is an excellent basic research material for citrus fruit trees.

[0003] At present, the domestic reports on Kakumquat are limited to its role as a model plant in basic research, and there are few reports on the research of reproduction mechanism. After the monoembryonic Kakumquat is sown, the individual traits of each offspring are obviously differentiated through gene recombination, and it is difficult to maintain the excellent traits of the mother plant. Asexual reproduction technology can maintain the excellent traits of the mother plant, which is an important basis for achieving efficient reproduction and stable inheritance of high-quality resources of monoembryonic Kakumquat.

[0004] Cutting propagation is a propagation method that uses the roots, stems, leaves and other propagation materials of trees for cuttings to cultivate independent new individuals. It is a commonly used propagation method in asexual reproduction, and has the advantages of being simple and easy, short seedling raising period, high propagation coefficient and strong seedlings. Propagation by cuttings of branches can better maintain the excellent traits of the parent of the mountain kumquat, shorten the propagation period, save labor, reduce production costs, and is simple and easy to implement. At the same time, it can improve land utilization and improve the survival rate of cuttings. At present, the propagation of mountain kumquat by cutting method faces the technical problem of difficult rooting. Therefore, providing a cutting propagation rooting method for mountain kumquat is a problem that those skilled in the art need to solve urgently. Summary of the invention

[0005] In order to solve the defects and shortcomings in the above-mentioned prior art, the present invention provides a method for rooting the cuttings of the kumquat, so that the kumquat has a higher rooting rate.

[0006] The technical solution of the present invention is: a method for rooting agarwood cuttings, which specifically comprises the following steps:

[0007] (1) Select one-year-old branches that are free of pests and diseases and have strong growth as cuttings;

[0008] (2) Trimming the selected cuttings as cuttings, each cutting has 2-3 half leaves, is 12-15 cm long, and is cut at a 45° angle;

[0009] (3) treating the pruned cuttings with a hormone solution;

[0010] (4) Insert the hormone-treated cuttings into the substrate.

[0011] Preferably, the cuttings selected on the same day are processed and then planted on the same day.

[0012] Preferably, the trimmed cuttings are rinsed with running water for 1-2 hours, dried and then treated with hormones.

[0013] Preferably, the hormone solution is NAA hormone solution, and the cuttings are treated by immersion.

[0014] Preferably, the cuttings soaking treatment is to place the cuttings in a hormone solution and soak them for 30 minutes.

[0015] Preferably, the concentration of the NAA hormone solution used is 400 mg / kg.

[0016] Preferably, the depth of the cuttings inserted into the substrate is 4-5 cm.

[0017] Preferably, the substrate is a common substrate, and its raw material is high-quality peat.

[0018] Preferably, the cuttings are placed in a greenhouse for management and the temperature is controlled at 25-35°C.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The invention discloses a cutting propagation and rooting method for kumquat. The method sequentially comprises the steps of selecting cuttings, pruning cuttings, treating with hormones and carrying out cuttings, so that the kumquat has a higher rooting rate and a larger number of rooted branches, shortens the propagation cycle, saves labor, reduces production costs, is simple and easy to operate, and simultaneously improves land utilization rate and planting survival rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The disclosure of the present invention will become more easily understood with reference to the accompanying drawings. It is easy for those skilled in the art to understand that these drawings are only used to illustrate the technical solutions of the present invention and are not intended to limit the protection scope of the present invention.

[0022] Figure 1 :Flowchart of the softwood cuttings of Kaempferia sempervivum;

[0023] Figure 2 : Rooting of cuttings treated with 400 mg / kg NAA for 30 minutes, 60 days after cuttings in March;

[0024] Figure 3 : Rooting of cuttings treated with 400 mg / kg NAA for 30 minutes, 60 days after cuttings in September;

[0025] Figure 4 : Rooting of cuttings treated with 400 mg / kg NAA for 30 minutes, 60 days after cuttings in April;

[0026] Figure 5 : Rooting of cuttings treated with 1000X rooting powder after 60 days;

[0027] Figure 6 : Rooting of cuttings treated with 400 mg / kg NAA for 30 min and with cutting soil as the cutting medium after 60 days;

[0028] Figure 7 : Representative diagrams of the five stages in the rooting process of kumquat softwood cuttings. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of this application.

[0030] Example 1

[0031] Figure 1 This is a flow chart of the rooting of kumquat cuttings used in the embodiment of the present invention, which mainly includes selecting cuttings, cutting pruning and hormone treatment, and the cuttings after hormone treatment are placed in a matrix for cutting rooting. The specific steps are as follows:

[0032] (1) In mid-March, select healthy, disease-free, and insect-free adult kumquat mother trees and collect one-year-old branches as cuttings;

[0033] (2) Trim the collected cuttings into cuttings with 2-3 half leaves and a length of 12-15 cm, and the lower incision of the cuttings is 45° oblique;

[0034] (3) Rinse the cuttings with running water for 1-2 hours for disinfection, take them out and dry them naturally in the shade; then soak them in 400 mg / kg NAA hormone solution for 30 minutes, and the lower incision should be completely immersed in the treatment solution during the treatment;

[0035] (4) Select a black pot with a pot model of 8.8*32*11 (cm), put the seedling medium into the black pot, the raw material of the seedling medium is high-quality carbon mud; when cutting, first use a wooden stick slightly thicker than the cutting to make a hole in the medium, then insert the lower end of the cutting into the medium along the hole, the cutting depth is 4-5cm, insert one cutting into each pot, and then gently compact the medium; water the cutting immediately after cutting, so that a layer of water film remains on the surface of the leaves;

[0036] (5) After cuttings, place them in a greenhouse and water them irregularly according to weather conditions. The temperature should be kept at the natural room temperature of 25-35°C. Check and remove rotten and dead cuttings and yellow and fallen leaves every 7-10 days after cuttings, and water them every two or three days depending on the moisture content of the substrate.

[0037] 112 days after cutting, the rooting results were counted, including survival rate (%), rooting rate (%), average number of roots (roots), and average root length (cm). Survival rate = number of surviving cuttings / total number of plants × 100%; rooting rate = number of rooted cuttings / total number of plants × 100%; average number of roots = total number of rooted cuttings / number of rooted plants; average root length = total root length of cuttings / number of rooted plants. Cuttings treated with 400 mg / kg NAA for 30 minutes had an average rooting rate of 60.0% after 112 days, an average of 2.59 roots per cutting, and an average root length of 4.65 cm.

[0038] Comparative Example 1

[0039] The steps of rooting of Kumquat cuttings adopted in the comparative example of the present invention are similar to those of Example 1, and mainly include selecting cuttings, pruning cuttings and treating with hormones, and then placing the cuttings treated with hormones into a matrix for rooting. Among them, in terms of the time of selecting cuttings, the comparative example selected cuttings of different time periods for cuttings; the pruning method of cuttings was the same as that of Example 1; when hormones were used to treat the cuttings, the comparative example of the present invention used different hormone types, concentrations and different soaking times to treat the pruned Kumquat cuttings; the hormone-treated cuttings were placed into different matrices for cultivation. Table 1 shows the tender branches of Kumquat treated with different hormone types and concentrations, different soaking times and different cutting matrices. Table 1 Treatment of tender branches of Kumquat with different hormone types and concentrations, different soaking times and different cutting matrices.

[0040] Table 1 Treatment of golden orange tender branches with different hormone types and concentrations, different soaking times, and different cutting media

[0041]

[0042] The treatment methods in Table 1 were used to obtain the survival rate and rooting rate of the softwood cuttings of the golden orange under different treatment conditions. Table 2 shows the statistical results of the survival rate and rooting rate of the softwood cuttings of the golden orange under different treatment conditions. The comparative method was used to root the golden orange cuttings, and the results of some treatment methods after 60 days were as follows: Figure 2-6 shown. Figure 7 This is a representative diagram of the five stages in the rooting process of kumquat softwood cuttings.

[0043] Table 2 Effects of different treatments on the survival rate and rooting rate of softwood cuttings of Citrus aurantium

[0044] Processing code Survival rate after 60 days (%) Rooting rate after 60 days (%) T1 76.67 0.00 T2 73.33 0.00 T3 83.33 3.33 T4 (Example 1) 75.56 15.56 T5 53.33 10.00 T6 20.83 0.00 T7 100.00 6.66 T8 83.33 0.00 T9 83.33 0.00 T10 76.67 0.00 T11 83.33 0.00 T12 80.00 0.00 T13 86.67 0.00 T14 70.00 0.00 T15 70.00 0.00 T16 66.67 0.00 T17 66.67 10.00 T18 0.00 0.00

[0045] As can be seen from Table 2, although the cuttings of Kaempferia ovata are easy to survive, it is difficult to root. The survival and rooting of the cuttings treated with NAA are better than those treated with IBA, ABT1 and rooting powder. Therefore, it is recommended that the cuttings used in subsequent experiments be treated with 400 mg / kg NAA for 30 minutes. After the cuttings were treated with 400 mg / kg NAA for 30 minutes, the cuttings in March performed better than those in April, September and October. Therefore, it is recommended to use the rooting data in March to improve the rooting ability of Kaempferia ovata tender branch cuttings. At the same time, among different cutting media, the cuttings treated with NAA hormones will have better rooting when the seedling medium (the raw material is high-quality carbon mud) is used. Therefore, after the above comparison, it can be seen that the cuttings treated with 400 mg / kg NAA for 30 minutes and the cuttings in March are cultivated with seedling medium, which can keep the survival rate of Kaempferia ovata after cuttings and have a higher rooting rate.

[0046] The above contents are further detailed descriptions of the present invention in combination with specific / preferred implementations, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, they can also make several substitutions or modifications to these described embodiments without departing from the concept of the present invention, and these substitutions or modifications should be regarded as belonging to the protection scope of the present invention.

Claims

1. A method for rooting kumquat cuttings, characterized in that: The following steps are involved: (1) selecting disease-free and insect-free branches with healthy growth as cuttings; (2) pruning the selected cuttings as cuttings; (3) treating the pruned cuttings with a hormone solution; (4) inserting the hormone-treated cuttings into a substrate.

2. The method for rooting axillaris cuttings according to claim 1, characterized in that: In the step (1), the selected branches are one-year-old branches.

3. The method for rooting agarwood cuttings according to claim 1, characterized in that: In the step (2), the trimmed cuttings have 2-3 half leaves, are 12-15 cm long, and are cut at a 45° angle.

4. The method for rooting agarwood cuttings according to claim 1, characterized in that: The cuttings trimmed in step (2) are rinsed with running water for 1-2 hours, dried and then treated with hormones. The cuttings selected on the same day are treated on the same day and then planted.

5. The method for rooting of Kumquat cuttings according to claim 1, characterized in that: The hormone solution in step (3) is NAA hormone solution, and the method for treating the cuttings is immersion method.

6. The method for rooting cuttings of Kumquat according to claim 5, characterized in that: Soak the cuttings in the hormone solution for 30 minutes.

7. The method for rooting agarwood cuttings according to claim 5, characterized in that: The concentration of the NAA hormone solution is 400 mg / kg.

8. The method for rooting cuttings of Citrus aureus according to claim 1, characterized in that: In step (4), the depth of the cuttings inserted into the substrate is 4-5 cm.

9. The method for rooting agarwood cuttings according to claim 1, characterized in that: The substrate used in step (4) is a common substrate, and its raw material is high-quality peat.

10. The method for rooting cuttings of Citrus aurantium according to claim 1, characterized in that: Place the grafted cuttings in a greenhouse for management and control the temperature at 25-35℃.

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