A leaf cutting propagation method for Potentilla rubra

Abstract

The present invention provides a leaf cutting propagation method of berry leaf Potentilla, comprising the steps of: using mature basal leaves of berry leaf Potentilla as cuttings, soaking in a hormone solution after disinfection, and cutting in a matrix after hormone treatment, wherein the hormone solution is 100-1000mg / L IBA solution or a mixed solution of 100-1000mg / L NAA and IBA, and the matrix is ​​peat, perlite, vermiculite mixed matrix, peat, perlite mixed matrix or peat, vermiculite mixed matrix. In the present invention, the cuttings can take root after 30d of leaf cutting of berry leaf Potentilla, and a complete root system can be formed after 45d, with a rooting rate of up to 63.33%, an average number of roots per plant of 6.90 / spike, an average root length of 27.98cm, a maximum root length of 13.83cm, and a root system effect index of 20.50. The invention effectively improves the rooting rate and rooting effect of the berry-leaf Potentilla, and at the same time makes up for the disadvantage of its long natural reproduction cycle. It is suitable for the cutting seedling cultivation of the berry-leaf Potentilla, and can provide a new way for the production and reproduction of the berry-leaf Potentilla.

Description

Technical Field

[0001] The invention belongs to the field of plant asexual reproduction, and particularly relates to a leaf cutting propagation method for Potentilla rubra. Background Art

[0002] The world is currently facing severe challenges from climate change. Problems such as resource shortages, environmental pollution, and ecosystem degradation are threatening human survival, and the pressure on the ecological environment is increasing day by day. In response to this, my country has made major decisions and deployments in recent years to "vigorously promote the construction of ecological civilization" and "build a beautiful China". Ecologically conservation-oriented gardens, as an important part of ecological civilization construction, have become a research hotspot and development direction of the current garden industry. However, lawns have long been used as ground cover plants in urban green spaces, which have high maintenance costs and consume a lot of water resources, and cannot meet the requirements of conservation-oriented garden construction. Therefore, seeking water-saving, drought-resistant, and adaptable native ground cover plants is an important direction for the construction of ecologically conservation-oriented gardens.

[0003] Among the native ground cover plant resources in northern my country, the Potentilla genus of the Rosaceae family is considered to be a native ground cover plant with development potential because of its strong stress resistance, extensive management, and wildness. There are many species of Potentilla plants, with more than 80 species distributed in various provinces and regions in my country, of which 21 species (variants) are recorded in Beijing. The silky creeping Potentilla and creeping Potentilla were included in the "List of Water-Saving and Drought-Tolerant Plants Suitable for Beijing" in 2019 and promoted and applied in Beijing. Previous investigations have found that most plants in the genus Potentilla have well-developed root systems, strong water storage and soil conservation capabilities, and high ornamental value. Some plant species have shown good ecological benefits and landscape effects in garden applications, but there are currently few species widely used in landscaping. The reason is that most species do not have mature breeding techniques.

[0004] Potentilla fragarioidis is widely distributed in the north and south of my country and is one of the species with excellent quality in the genus Potentilla. During its growth period, it often has a hemispherical crown and a beautiful plant shape. It is a spring flower plant with large flowers and bright colors. It can be used in perennial flower beds or flower borders, and is also suitable for patch planting. It has a good landscape effect as a ground cover plant for viewing flowers and leaves in northern my country. The green period is more than 250 days. In Beijing, it has entered the green period in early March. It can survive the winter without any protective measures. It has strong resistance to diseases and pests and is cold-resistant and drought-resistant. It is a northern native ground cover plant worthy of promotion and application. In addition, Potentilla fragarioidis is rich in high protein, high fiber and various essential trace elements. The effective ingredient d-catechin has multiple functions such as preventing and treating cardiovascular diseases and cancer. It is also often eaten as a wild vegetable by the people, and has both edible and medicinal value. At present, the berry-leaved Potentilla is commonly propagated by division or sowing, but its seeds are easy to scatter by themselves after maturity and are not easy to collect. The seed germination rate in the natural state is less than 40%, and the seedling raising period is long; although division propagation can shorten the seedling raising period, the overall reproduction coefficient is low, and the seedlings mainly come from wild resources, which cannot meet the needs of garden applications in the long run. Therefore, the berry-leaved Potentilla has not yet been widely used in gardening. As for the cutting propagation technology, the existing literature only explores the Potentilla plants with runners, such as Potentilla indica, Potentilla velvet and Potentilla vine, and most of them choose runner segments as cuttings. There is no report on the research on the leaf cutting propagation technology of Potentilla.

[0005] Although Potentilla rubra has no above-ground stems, its basal leaves grow in large quantities and quickly. As long as the environmental conditions are suitable, new leaves can be produced throughout the year. It is believed that using its basal leaves for cuttings may become a new way for asexual reproduction of Potentilla rubra. In order to promote the application of Potentilla rubra in landscaping, this study conducted an orthogonal test of different cutting substrates, hormone types and concentrations, and compared the effects of different treatments on the rooting effect of leaf cuttings, so as to find the best treatment combination suitable for leaf cuttings of Potentilla rubra, and provide a new way for its seedling breeding and production. Summary of the invention

[0006] In order to solve the above problems, the present application aims to provide a leaf cutting propagation method of Potentilla tataricola.

[0007] The leaf cutting propagation method of Potentilla rubra provided by the present invention comprises the following steps: using mature basal leaves of Potentilla rubra as cuttings, immersing the cuttings in a hormone solution after disinfection, and inserting the cuttings into a matrix after hormone treatment, wherein the hormone solution is a 100-1000 mg / L IBA solution or a 100-1000 mg / L mixed solution of NAA and IBA, and the matrix is ​​a mixed matrix of peat, perlite and vermiculite, a mixed matrix of peat and perlite, or a mixed matrix of peat and vermiculite.

[0008] Wherein, the hormone solution is a 300-500 mg / L IBA solution, or a 100-500 mg / L NAA and IBA mixed solution.

[0009] The soaking time of the hormone solution is 10-30 minutes, preferably 20 minutes.

[0010] The matrix is ​​a mixed matrix of peat, perlite and vermiculite in a volume ratio of 1:1:1, a mixed matrix of peat and perlite in a volume ratio of 1:1, or a mixed matrix of peat and vermiculite in a volume ratio of 1:1, preferably a mixed matrix of peat, perlite and vermiculite in a volume ratio of 1:1:1.

[0011] In a preferred embodiment of the present invention, the hormone solution is a 300 mg / L IBA solution, and the matrix is ​​a mixed matrix of peat, perlite and vermiculite in a volume ratio of 1:1:1.

[0012] In a specific embodiment of the present invention, the leaf cutting propagation method also includes a method for processing cuttings: on a cloudy morning, cut mature basal leaves that are healthy, free of pests and diseases, and have uniform growth as cuttings, with a petiole length of 6 to 8 cm, retaining only the three leaflets at the top of the pinnate compound leaves, and trimming off 1 / 2 of the leaves, and removing all the remaining leaflets. The cut at the base of the cuttings is a 45° section. After processing, place them in a carbendazim solution and soak them for disinfection for 10 minutes, and then rinse them under running water.

[0013] Among them, when cutting, the insertion depth should be controlled at 3 to 4 cm, and the cuttings should be gently pressed after insertion.

[0014] After the cuttings are completed, the leaves are sprayed with water and covered with a film to keep moisture. Rooting culture is carried out under the conditions of 20-25°C and 12-16h / d of light exposure, and the relative humidity of the environment is maintained at 80-90%.

[0015] Among them, keep the substrate soil moist during the cutting period, spray the cutting bed and cuttings with 800 times of carbendazim every 2 weeks to prevent mildew and rot, clean up dead branches and leaves regularly to keep the cutting bed clean.

[0016] In the present invention, the cuttings of the berry-leafed Potentilla can take root after 30 days of leaf cutting, and a complete root system can be formed after 45 days, the rooting rate can reach 63.33%, the average number of roots per plant is 6.90 per cutting, the average root length is 27.98 cm, the longest root length is 13.83 cm, and the root system effect index reaches 20.50. The present invention effectively improves the rooting rate and rooting effect of the berry-leafed Potentilla, and at the same time makes up for the disadvantage of its long natural reproduction cycle, is suitable for the cutting seedling cultivation of the berry-leafed Potentilla, and can provide a new way for the production and reproduction of the berry-leafed Potentilla. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Shown are the results of leaf cuttings of Potentilla oleifera after being treated with clean water.

[0018] Figure 2 The figure shows the rooting process of leaf cuttings of Potentilla oleracea. In the figure, a. white callus tissue is formed; b. callus tissue gradually expands; c. callus roots are differentiated; d. callus roots gradually elongate and develop into a root system

[0019] Figure 3 Shown is the rooting effect of cuttings in treatment T15. DETAILED DESCRIPTION

[0020] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0021] Example 1

[0022] 1. Cutting preparation

[0023] The experimental materials are two-year-old seedlings of Potentilla tataricola. Mature basal leaves that are healthy, pest-free, and have uniform growth are used as cuttings. The petiole length is 6 to 8 cm. Only the three leaflets at the top of the pinnate compound leaves are retained, and 1 / 2 of the leaves are trimmed off. The remaining leaflets are all removed. The base of the cuttings is cut at a 45° section. After processing, they are first soaked in a diluted carbendazim solution for 10 minutes, then rinsed under running water, and then placed in clean water to keep them moist.

[0024] 2. Matrix Preparation

[0025] One day before cutting, peat and perlite were mixed in equal volume ratios according to the experimental design, the mixed substrate was disinfected with 800 times carbendazim solution, and the substrate was rinsed with clean water. Then, the substrate was placed in the plug tray for use.

[0026] 3 Methods and Results

[0027] 3.1 Test methods

[0028] The experiment used peat and perlite mixed in equal proportions as the cutting medium, and no hormones were used to treat the cuttings. Each treatment had 32 cuttings, and was repeated 3 times. After 45 days of cutting, the seedlings were removed from the whole tray for measurement, and the rooting rate (%), mortality rate (%) and cutting status were statistically recorded.

[0029] 3.2 Test results

[0030] After 45 days of cutting, all cuttings failed to take root (rooting rate 0.00%), the cutting mortality rate was 70.83%, and the surviving cuttings were in poor condition, with leaves and petioles losing water and brown spots ( Figure 1 ).

[0031] Example 2

[0032] 1. Cutting preparation

[0033] The experimental material is two-year-old seedlings of Potentilla tataricola. In the early morning of cloudy days in summer (July-August), mature basal leaves that are healthy, free of pests and diseases, and have uniform growth are cut as cuttings. The petiole length is 6-8 cm. Only the leaves above the top growth point are retained and 1 / 2 of the leaves are trimmed. The remaining leaves are all removed. The cut at the base of the cuttings is a 45° section. After treatment, they are first soaked in a diluted carbendazim solution for 10 minutes, and then rinsed under running water.

[0034] 2. Matrix Preparation

[0035] One day before cutting, peat, vermiculite and perlite were mixed in different proportions (volume ratio) according to the experimental design, the mixed matrix was disinfected with 800 times carbendazim solution, and the matrix was rinsed with clean water, and then the matrix was placed in the plug tray for use.

[0036] 3 Methods and Results

[0037] 3.1 Test methods

[0038] Cutting medium, hormone type and hormone concentration were used as experimental factors, and each factor was set at 4 levels. 16 (4 5 ) Orthogonal experimental design (Table 1) was used, with 16 treatments, 10 cuttings per treatment, and repeated 3 times. The hormone treatment time was 20 min. After 45 days of cutting, all seedlings were removed and tested, and the rooting rate (%), average number of roots per plant, average root length (cm), longest root length (cm), root system effect index and other indicators and cutting status of the 16 treatments were statistically recorded.

[0039] Table 1L 16 (4 5 ) Orthogonal Experimental Design

[0040]

[0041] The cuttings were made by direct insertion. According to the experimental design, 2 to 4 cm from one end of the base of the prepared cuttings was immersed in the corresponding hormone for 20 minutes. The insertion depth was controlled at about 3 to 4 cm during cuttings, and the cuttings were gently pressed after insertion. After the cuttings were completed, the leaves were uniformly sprayed with water once and covered with a moisturizing cover. Rooting culture was carried out for 45 days at 20°C and 16h / d of light time, and the relative humidity of the environment was maintained at 80 to 90%.

[0042] After cutting, the temperature and humidity should be controlled. Watering should be carried out according to the substrate conditions and the degree of leaf wilting. Spray the leaves with a sprayer every day for 3 days before cutting, and then water thoroughly once every week. Keep the substrate soil moist at ordinary times. Spray the cutting bed and cuttings with 800 times carbendazim every 2 weeks to prevent mildew and rot. Clean up dead branches and fallen leaves regularly to keep the cutting bed clean.

[0043] 3.2 Test results

[0044] Through continuous observation of the rooting of leaf cuttings of Potentilla rubra, it was found that 2 to 3 weeks after cutting, cracks appeared at the base of the cuttings, and the lower incision gradually expanded to form white callus tissue ( Figure 2 a); 4 to 6 weeks, the callus gradually expands ( Figure 2 b), and then differentiate into callus roots ( Figure 2 c) and gradually develop into a root system ( Figure 2 d).

[0045] The results of variance analysis of rooting indicators showed (Table 2) that the cutting medium had a very significant effect on the rooting rate of leaf cuttings of Potentilla rubra (P<0.01), and also had a significant effect on the average number of roots per plant (P<0.05); the type of hormone had a very significant effect on all test indicators (P<0.01); and the hormone concentration only had a significant effect on the average root length (P<0.05).

[0046] Table 2 Analysis of variance

[0047]

[0048] Note: A: cutting medium; B: hormone type; C: hormone concentration. ** and * indicate significant differences at the 0.01 and 0.05 levels, respectively.

[0049] As shown in Table 3, after 45 days of cutting, all treatments except treatment T10 had roots, and T7, T15 and T16 performed best, with rooting rates of 66.67%, 63.33% and 60.00% respectively, and there was no significant difference among the three, while the rooting rates of T5, T9 and T10 were all lower than 10.00%. The average number of roots per plant varied from 0 to 7.75 per ear, with the largest number of roots in treatment T8, which was 7.75 per ear, followed by T7 and T15, which were 6.91 and 6.90 per ear respectively, and there was no significant difference among the three. In terms of average root length and maximum root length, the range of variation of the 16 treatments was 0-35.10 cm and 0-15.69 cm, respectively. Among them, the average root length and maximum root length of T11 were the largest. Except for the treatment T10 without rooting, the average root length and maximum root length of T5 were the smallest, which were 0.86 cm and 0.79 cm, respectively. The root effect index varied from 0 to 22.71, among which T11 had the largest root effect index, which was 22.71, followed by T15 and T8, which were 20.50 and 16.58, respectively. Except for the treatment T10 without rooting, the root effect index of T5 was the smallest, which was 0.16.

[0050] Although T8 had the largest average number of roots per plant, its rooting rate (23.33%) was much lower than that of T7 (66.67%) and T15 (63.33%); T11 had the largest root efficiency index, average root length and longest root length, but its rooting rate was only 13.33%. The average root length and root efficiency index of T15 were second only to T11, and the longest root length was not significantly different from that of T11, while the rooting rate (63.33%) was much higher than that of T11.

[0051] Table 3 Rooting indexes of leaf cuttings of Potentilla oleifera under different treatments

[0052]

[0053] Note: Data are mean ± standard error; different lowercase letters in the same column indicate significant differences (P < 0.05).

[0054] In order to comprehensively evaluate the rooting status of leaf cuttings of Potentilla berry leaves, and further explore the rooting effects of cuttings under different cutting media, hormone types and concentrations, the principal component analysis method was used to comprehensively evaluate the five rooting indices. As can be seen from Table 4, the cumulative contribution rate of the first and second principal components is 84.19%. According to the principle of cumulative contribution rate of variance>80%, the principal components are extracted. It can be seen that the information is mainly concentrated in the first two principal components, so the first two principal components are selected to reflect the information content of the rooting effect of leaf cuttings of Potentilla berry leaves.

[0055] Table 4 Eigenvalues ​​and principal component contribution rates and cumulative contribution rates

[0056]

[0057] The expression formula of each principal component is:

[0058] Y 1 =0.284X 1 +0.458X 2 +0.512X 3 +0.444X 4 +0.500X 5

[0059] Y 2 =0.788X 1 -0.235X 2 -0.171X 3 +0.376X 4 -0.392X 5

[0060] Y 1 , Y 2 The two principal components and their variance contribution rates construct a comprehensive evaluation model Z for the rooting effect of Potentilla oleracea cuttings. Z is the principal component Y 1 , Y 2 The linear combination of is Z = 0.6406Y 1 +0.2013Y 2 , and the five rooting indices were comprehensively evaluated using this expression.

[0061] As shown in Table 5, treatment T15 (peat + perlite + vermiculite (1:1:1), 300 mg·L -1 The comprehensive score of treatment T11 (peat + perlite (1:1), 100 mg·L - 1 IBA treatment), treatment T3 (peat + vermiculite (1:1), 500 mg·L -1 IBA treatment), treatment T7 (vermiculite + perlite (1:1), 1000 mg·L -1 IBA treatment) ranked 2nd, 3rd, and 4th, respectively, with comprehensive scores all exceeding 1.00. The worst rooting effect was treatment T9 (peat + perlite (1:1), 1000 mg·L -1 ABT), treatment T5 (vermiculite + perlite (1:1), 300 mg·L -1 ABT), treatment T10 (peat + perlite (1:1), 1000 mg·L -1 The results of principal component analysis showed that IBA was the best hormone for the treatment of cuttings of Potentilla oleracea, 100-1000 mg·L -1The rooting effect of cuttings treated with IBA was better than that of other hormones. -1 is the optimal hormone concentration.

[0062] Table 5 Comprehensive evaluation of the rooting effect of leaf cuttings of Potentilla oleifera under different treatments

[0063]

[0064] In summary, peat + perlite + vermiculite (1:1:1) was used as the matrix, and 300 mg·L -1 The rooting effect of cuttings was best when the cuttings were treated with IBA for 20 minutes. Figure 3 ).

[0065] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A leaf cutting propagation method for Potentilla rubra, characterized in that: The method comprises the following steps: cutting healthy, pest-free and uniformly growing mature basal leaves of Potentilla rubra in the early morning on a cloudy day as cuttings, with a petiole length of 6-8 cm, retaining only the three leaflets at the top of the pinnate compound leaves, trimming off 1 / 2 of the leaves, and removing all the other leaflets, with the cuttings having a 45° cut surface at the base, disinfecting the cuttings after the treatment, immersing the cuttings in a hormone solution, and implanting the cuttings in a matrix after the hormone treatment, wherein the hormone solution is a 100-1000 mg / L IBA solution, and the matrix is ​​a mixed matrix of peat, perlite and vermiculite.

2. The leaf cutting propagation method according to claim 1, characterized in that: The hormone solution is a 300-500 mg / L IBA solution.

3. The leaf cutting propagation method according to claim 1, characterized in that: The time for the hormone solution soaking treatment is 10-30 minutes.

4. The leaf cutting propagation method according to claim 1, characterized in that: The matrix is ​​a mixed matrix of peat, perlite and vermiculite in a volume ratio of 1:1:

1.

5. The leaf cutting propagation method according to claim 1, characterized in that: The hormone solution is a 300 mg / L IBA solution, and the matrix is ​​a mixed matrix of peat, perlite and vermiculite in a volume ratio of 1:1:

1.

6. The leaf cutting propagation method according to any one of claims 1 to 5, characterized in that: The disinfection treatment is to first immerse the cuttings in a carbendazim solution for 10 minutes for disinfection, and then rinse them under running water.

7. The leaf cutting propagation method according to any one of claims 1 to 5, characterized in that: When cutting, control the insertion depth to 3~4 cm, and gently press the cuttings after insertion.

8. The leaf cutting propagation method according to any one of claims 1 to 5, characterized in that: After the cuttings are completed, spray water on the leaves and cover them with a film to keep them moist. Carry out rooting culture under the conditions of 20-25℃ and 12-16 h / d of light time, and keep the relative humidity of the environment at 80~90%.

9. The leaf cutting propagation method according to any one of claims 1 to 5, characterized in that: Keep the substrate soil moist during the cutting period, spray the cutting bed and cuttings with 800 times of carbendazim every 2 weeks to prevent mildew and rot, clean up dead branches and leaves regularly to keep the cutting bed clean.

Images