Method suitable for indoor tea tree scion water culture cutting rooting and germination and suitable for mechanical picking

Abstract

According to the hydroponic nutrient solution suitable for indoor soilless cutting propagation of the tea trees and the method for performing cutting rooting, germination and mechanical picking by using the hydroponic nutrient solution provided by the invention, by using the nutrient solution and the conditioning agent, rapid rooting and periodic germination of the hydroponic tea seedlings can be realized, and new shoots of the tea seedlings are suitable for mechanical picking; therefore, the problems of poor rooting rate of tea tree cutting cuttage, irregular germination and mechanical shoot picking are effectively solved.

Description

Technical Field

[0001] The invention belongs to the field of agricultural technology, specifically the field of tea tree cultivation, and more specifically provides a method suitable for indoor tea tree fringe cutting rooting, germination and mechanical harvesting. Background Art

[0002] Tea trees belong to the genus Camellia of the Theaceae family. They are perennial evergreen woody plants and one of the most important economic crops in my country. There are two ways to propagate tea trees. One is sexual reproduction, which is seed reproduction through cross-pollination and hybridization. The advantages of this method of reproduction are fast rooting and high survival rate of seeds, but the disadvantage is that it is difficult to maintain the excellent traits of the parents. The other method is propagation by cuttings, which is also the main method of breeding asexual tea varieties in production. Short-spiked cuttings can inherit the excellent traits of asexual varieties, are not easy to mutate, and are simple to operate. At present, soil or solid nutrient soil matrix is ​​mainly used to propagate tea cutting seedlings in production. The main problem is that rooting is difficult, and it is greatly affected by the external environment, the propagation coefficient is low, and it can only be propagated 1 to 2 times a year. In addition, the use of solid culture methods such as traditional soil requires a lot of labor, and tea trees like acidity and have high requirements for soil pH and organic matter content.

[0003] Propagating tea trees by cuttings with nutrient solution hydroponics in a greenhouse is a more efficient, time-saving and cost-saving method. Its advantage is that external environmental factors such as light, temperature, CO2, water and pH can be artificially controlled in the greenhouse to avoid the invasion of tea seedlings by some diseases and pests transmitted through the soil. At the same time, by accurately controlling the nutrients required for the rooting and germination of tea seedlings, the seedlings can be quickly rooted and sprouted. In addition, through reasonable pruning and hydroponic methods, the budding period of tea trees can also be accurately controlled to achieve mechanized harvesting. Therefore, the development of nutrient solutions, growth regulators and suitable pruning methods suitable for tea tree hydroponics is of great significance for the rapid cutting, germination and tender bud harvesting of tea seedlings. Summary of the invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a hydroponic nutrient solution suitable for soilless cutting propagation of indoor tea trees and a method of using the same for rooting, sprouting and machine picking of cuttings. Through the use of nutrient solution and regulators, hydroponics can achieve rapid rooting of tea seedlings, periodic sprouting and new shoots of tea seedlings suitable for machine picking, thereby effectively solving the problems of poor rooting rate of tea tree cuttings, uneven sprouting and machine picking of new shoots.

[0005] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0006] A nutrient solution A for indoor hydroponics of tea tree cuttings, the nutrient solution A comprising the following nutrients: (1) macroelements: nitrogen (N) 1 mmol / L, phosphorus (P) 0.05 mmol / L, potassium (K) 0.35 mmol / L, calcium (Ca) 0.395 mmol / L, magnesium (Mg) 0.21 mmol / L, aluminum (AL) 0.25 mmol / L; (2) iron salts: iron (Fe) 2.1 μmol / L, sodium (Na) 2.1 μmol / L; (3) trace elements: boron (B) 3.33 μmol / L, zinc (Zn) 0.51 μmol / L, manganese (Mn) 0.5 μmol / L, molybdenum (Mo) 0.17 μmol / L, copper (Cu) 0.13 μmol / L.

[0007] A growth regulator B for indoor tea tree cutting rooting by hydroponic cuttings, the growth regulator B comprising the following nutrients: 0.5 mg / L NAA+1 mg / L IBA, wherein NAA is naphthylacetic acid and IBA is indolebutyric acid.

[0008] A growth regulator C for indoor tea tree cuttings hydroponic germination, the growth regulator C comprising the following nutrients: 4 mg / L 6-BA+0.2 mg / L IBA, wherein 6-BA is 6-benzylaminopurine, and IBA is indolebutyric acid.

[0009] A method suitable for indoor tea tree cuttings hydroponic rooting, germination and mechanical harvesting, comprising the following steps:

[0010] 1) Branch preparation: Collect the semi-lignified new shoots of the tea tree of the year, soak them in a carbendazim solution, and then wash off the surface soil and other impurities with clean water;

[0011] 2) Cutting pruning: Use pruning shears to cut off the cuttings on the new shoots one by one. Each cutting carries a mature leaf. Remove the cuttings with pests and diseases. Then cut off 2 / 3 of the mature leaves on the cuttings, and select cuttings of qualified size for cuttings;

[0012] 3) Cutting board production: Use thickened EPE pearl cotton foam board as the cutting board, and draw the spacing of the cuttings on the cutting board;

[0013] 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box;

[0014] 5) Prepare nutrient solution A, growth regulator B and growth regulator C;

[0015] 6) Add nutrient solution A to the hydroponic box, place the cuttage cuttings into the hydroponic box, ensure that each cutting can be exposed to the nutrient solution, and replace with new nutrient solution A after 7 consecutive days of cultivation, and add growth regulator B, and continue to cultivate until new roots grow, during which the culture solution is replaced every 7 days;

[0016] 7) Germination: After new roots grow out, growth regulator C can be added to nutrient solution A. New buds will begin to sprout after about a week. If buds are to sprout first, add growth regulator C first in the above steps.

[0017] 8) Machine picking: When the young spikelets reach one bud and one leaf or one bud and two leaves, use a handheld rechargeable small tea picking machine to harvest the new shoots;

[0018] 9) Re-germination: After the new shoots are harvested, replace with new nutrient solution A. After 2 weeks, add growth regulator C to the nutrient solution. After about 1 week, the new shoots begin to sprout. When they reach the harvesting standard, use a tea picking machine to harvest the new shoots.

[0019] Preferably, the method suitable for indoor tea tree cuttings hydroponic rooting, germination and mechanical harvesting comprises the following steps:

[0020] 1) Branch preparation: Collect the semi-lignified new shoots of tea trees of the current year, soak them in 1% carbendazim solution for 2 hours, and then wash off the surface soil and other impurities with clean water;

[0021] 2) Spike pruning: Use pruning shears to cut off the spikes on the new shoots one by one. The length of the spikes is about 4 cm. Each spike carries a mature leaf. Remove the spikes with pests and diseases, and then cut off 2 / 3 of the mature leaves on the spikes. Select spikes of qualified size for cuttings;

[0022] 3) Cutting board production: Use thickened EPE pearl cotton foam board as the cutting board, cut the size to length × width × thickness = 45cm × 30cm × 1.5cm, use a black silk marker to draw the cutting row spacing of 3 × 3cm on the cutting board, and the number of spikes set on each cutting board is 140;

[0023] 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box, the box size is 45×30×15cm;

[0024] 5) Prepare nutrient solution A, growth regulator B and growth regulator C;

[0025] 6) Add nutrient solution A to the hydroponic box, place the cuttage cuttings into the hydroponic box, ensure that each cutting can be exposed to the nutrient solution, and replace with new nutrient solution A after 7 consecutive days of cultivation, and add growth regulator B, and continue to cultivate until new roots grow, during which the culture solution is replaced every 7 days;

[0026] 7) Germination: After new roots grow out, growth regulator C can be added to nutrient solution A. New buds will begin to sprout after about a week. If buds are to sprout first, add growth regulator C first in the above steps.

[0027] 8) Machine picking: When the young spikelets reach one bud and one leaf or one bud and two leaves, use a handheld rechargeable small tea picking machine to harvest the new shoots;

[0028] 9) Re-germination: After the new shoots are harvested, replace with new nutrient solution A. After 2 weeks, add growth regulator C to the nutrient solution. After about 1 week, the new shoots begin to sprout. When they reach the harvesting standard, use a tea picking machine to harvest the new shoots.

[0029] The preparation method of the nutrient solution A is as follows: weigh the macroelements ammonium sulfate, calcium nitrate tetrahydrate, potassium dihydrogen phosphate, potassium sulfate, calcium chloride, magnesium sulfate, and aluminum sulfate respectively in proportion to prepare 1 liter of mother liquor; weigh the iron salt EDTA and iron sulfate in proportion to mix and prepare 1 liter of mother liquor; weigh the trace elements boric acid, zinc sulfate, copper sulfate, manganese sulfate, and sodium molybdate in proportion to mix and prepare 1 liter of mother liquor, and store it in a refrigerator at 4°C; the formula of the nutrient solution is that the ratio of macroelements: iron salts: trace elements is 4:1:1, pure water is used as the solvent, and a small amount of sodium hydroxide solution is added to make the pH value of the final nutrient solution between 4.5 and 5.5.

[0030] The preparation method of the growth regulator B is as follows: naphthylacetic acid and indolebutyric acid are weighed separately, mixed and dissolved in purified water, and the final concentrations thereof are 0.5 mg / L and 1 mg / L respectively when used.

[0031] The preparation method of the growth regulator C is as follows: 6-benzylaminopurine and indolebutyric acid are weighed separately, mixed and dissolved in purified water, and the final concentrations thereof are 4 mg / L and 0.2 mg / L respectively.

[0032] In the method described, the culture room is a glass greenhouse, the temperature is controlled at about 20-25°C, and the light source is natural light.

[0033] The beneficial effects of the present invention are as follows: the nutrient solution A provided by the present invention can meet the basic conditions for the growth of tea seedlings, the growth regulator B can promote the rapid rooting of tea spikelets, and the growth regulator C can stimulate the rapid germination of tea seedlings. Compared with the traditional hydroponic method, the present invention can not only significantly improve the rooting rate of tea spikelets and promote the early germination of tea spikelets, but also use this hydroponic method to achieve machine picking of tea shoots, providing a good solution for the difficulty of machine picking of high-quality tea, and also laying a certain foundation for the future full mechanization (seedling-picking-processing) production of tea. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is the rooting situation of the tea tree cuttings grown for 28 days in Example 1 of the present invention;

[0035] Figure 2 The germination of the tea tree cuttings after 28 days of growth in Example 2 of the present invention;

[0036] Figure 3 This is the situation when the tea tree cuttings grow to the harvesting requirements in Example 3 of the present invention;

[0037] Figure 4 The effect of adding nutrient solution and growth regulator on the growth of tea tree cuttings in the present invention;

[0038] Figure 5 The invention discloses the influence of adding nutrient solution and growth regulator on the budding of tea tree spikelets. DETAILED DESCRIPTION

[0039] Embodiment 1 A hydroponic method for rapid rooting of tea tree cuttings comprises the following steps:

[0040] 1) Branch preparation: Collect the semi-lignified new shoots of tea trees of the current year, soak them in 1% carbendazim solution for 2 hours, and then wash off the surface soil and other impurities with clean water;

[0041] 2) Cutting pruning: Use pruning shears to cut the cuttings from the new shoots one by one. The cuttings are about 4 cm long and each cutting carries a mature leaf. Cut off the cuttings with pests and diseases. Then cut off 2 / 3 of the mature leaves on the cuttings and select cuttings of qualified size for cuttings.

[0042] 3) Cutting board preparation: Use thickened EPE pearl cotton foam board as the cutting board, and cut the size to length × width × thickness = 9cm × 6cm × 1.5cm; Use a black silk marker to draw the cutting row spacing of 3×3cm on the cutting board, and the number of spikes set on each cutting board is 12;

[0043] 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box, the box size is 45×30×15cm;

[0044] 5) Preparation of nutrient solution A: Weigh 24.774 g of macroelements ammonium sulfate, 14.759 g of calcium nitrate tetrahydrate, 2.402 g of potassium dihydrogen phosphate, 13.069 g of potassium sulfate, 8.047 g of calcium chloride, 12.638 g of magnesium sulfate, and 41.65 g of aluminum sulfate to make 1 liter of mother solution; weigh 1.564 g of EDTA and 1.168 g of ferric sulfate to make 1 liter of mother solution; weigh 0.4178 g of boric acid, 0.2933 g of zinc sulfate, 0.0648 g of copper sulfate, 0.1690 g of manganese sulfate, and 0.0823 g of sodium molybdate to make 1 liter of mother solution, and store in a refrigerator at 4°C; the final formula of the nutrient solution is a final ratio of macroelements: iron salts: trace elements of 4:1:1. Use purified water as the solvent and add a small amount of sodium hydroxide solution to make the pH value of the final nutrient solution between 4.5 and 5.5;

[0045] 5) Preparation of growth regulator B: Weigh a certain amount of naphthaleneacetic acid (NAA) and indolebutyric acid (IBA), mix them and dissolve them in purified water. When using, the final concentration is as shown in Table 1.

[0046] 6) Add nutrient solution A to the hydroponic box, place the cut fringe in the hydroponic box, ensure that each fringe can contact the nutrient solution, and culture for 7 consecutive days; add growth regulator B to the new nutrient solution A, and replace the culture solution every 7 days;

[0047] 7) After 28 days of continuous cultivation, the rooting rate and number of roots were counted;

[0048] 8) Environmental conditions: The temperature of the glass greenhouse is controlled at around 20-25°C, the light source is natural light, and the humidity is around 75%.

[0049] The results showed that the rooting rate and number of rooted tea cuttings after adding different concentrations of growth regulator B were significantly increased compared with the control. Among the tea cuttings treated with 4 different concentrations, the rooting rate and number of rooted tea cuttings after T3 (NAA 1mg / L, IBA 0.5mg / L) treatment were better than those of other treatments. Therefore, the use of the growth regulator B in the present invention can significantly improve the rooting rate and number of rooted tea cuttings.

[0050] Table 1 Effects of different concentrations of NAA and IBA on germination of tea cuttings

[0051]

[0052]

[0053] Note: Each treatment was repeated 3 times. * indicates P<0.05, ** Indicates P<0.01.

[0054] Embodiment 2 A hydroponic method for rapid germination of tea tree cuttings after cuttings comprises the following steps:

[0055] 1) Branch preparation: Collect the semi-lignified new shoots of tea trees of the current year, soak them in 1% carbendazim solution for 2 hours, and then wash off the surface soil and other impurities with clean water;

[0056] 2) Cutting pruning: Use pruning shears to cut the cuttings from the new shoots one by one. The cuttings are about 4 cm long and each cutting carries a mature leaf. Cut off the cuttings with pests and diseases. Then cut off 2 / 3 of the mature leaves on the cuttings and select cuttings of qualified size for cuttings.

[0057] 3) Cutting board preparation: Use thickened EPE pearl cotton foam board as the cutting board, and cut the size to length × width × thickness = 9cm × 6cm × 1.5cm; Use a black silk marker to draw the cutting row spacing of 3×3cm on the cutting board, and the number of spikes set on each cutting board is 12;

[0058] 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box, the box size is 45×30×15cm;

[0059] 5) Preparation of nutrient solution A: Weigh 24.774 g of macroelements ammonium sulfate, 14.759 g of calcium nitrate tetrahydrate, 2.402 g of potassium dihydrogen phosphate, 13.069 g of potassium sulfate, 8.047 g of calcium chloride, 12.638 g of magnesium sulfate, and 41.65 g of aluminum sulfate to make 1 liter of mother solution; weigh 1.564 g of EDTA and 1.168 g of ferric sulfate to make 1 liter of mother solution; weigh 0.4178 g of boric acid, 0.2933 g of zinc sulfate, 0.0648 g of copper sulfate, 0.1690 g of manganese sulfate, and 0.0823 g of sodium molybdate to make 1 liter of mother solution, and store in a refrigerator at 4°C; the final formula of the nutrient solution is a final ratio of macroelements: iron salts: trace elements of 4:1:1. Use purified water as the solvent and add a small amount of sodium hydroxide solution to make the pH value of the final nutrient solution between 4.5 and 5.5;

[0060] 6) Preparation of growth regulator C: Weigh a certain amount of 6-benzylaminopurine (6-BA) and indolebutyric acid (IBA) respectively, and prepare different concentrations of growth regulators, as shown in Table 2;

[0061] 7) Add nutrient solution A to the hydroponic box, place the cut stalks into the hydroponic box, ensure that each stalk is in contact with the nutrient solution, and culture for 7 consecutive days; add growth regulator C to the new nutrient solution A, and replace the culture solution every 7 days;

[0062] 7) Environmental conditions. The temperature of the glass greenhouse is controlled at around 20-25°C, the light source is natural light, and the humidity is around 75%;

[0063] 8) After 28 days of continuous cultivation, the germination rate was counted.

[0064] The results showed that the germination rate of tea tree cuttings after adding growth regulator C was significantly increased compared with the control. Among the tea tree cuttings treated with 4 different concentrations, the regulator with T3 ratio was more advantageous, reaching a very significant effect compared with the control, and was the best among the 4 treatments. Therefore, the use of growth regulator C in the present invention can significantly increase the germination rate of tea tree cuttings.

[0065] Table 2 Effects of different concentrations of 6-BA and IBA on germination of tea cuttings

[0066] Group 6-BA(mg / L) IBA(mg / L) Germination rate (%) CK 0 0 41.67±8.33 T1 2 0.2 80.56±4.81** T2 2 0.6 69.44±9.62* T3 4 0.2 91.67±8.33** T4 4 0.6 72.22±4.81**

[0067] Note: Each treatment was repeated 3 times. * indicates P<0.05, ** Indicates P<0.01.

[0068] Embodiment 3 A method for hydroponic cutting rooting, germination and mechanical harvesting of tea tree cuttings, comprising the following steps:

[0069] 1) Branch preparation: Collect the semi-lignified new shoots of Longjing 43 tea tree variety, soak them in 1% carbendazim solution for 2 hours, and then wash off the surface soil and other impurities with clean water;

[0070] 2) Cutting pruning: Use pruning shears to cut the cuttings from the new shoots one by one. The cuttings are about 4 cm long and each cutting carries a mature leaf. Cut off the cuttings with pests and diseases. Then cut off 2 / 3 of the mature leaves on the cuttings and select cuttings of qualified size for cuttings.

[0071] 3) Cutting board preparation: Use thickened EPE pearl cotton foam board as the cutting board, and cut the size to length × width × thickness = 45cm × 30cm × 1.5cm; Use a black silk marker to draw the cutting row spacing of 3×3cm on the cutting board, and the number of spikes set on each cutting board is 140;

[0072] 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box, the box size is 45×30×15cm, and the culture solution is 10L;

[0073] 5) Preparation of nutrient solution A: Weigh 24.774 g of macroelements ammonium sulfate, 14.759 g of calcium nitrate tetrahydrate, 2.402 g of potassium dihydrogen phosphate, 13.069 g of potassium sulfate, 8.047 g of calcium chloride, 12.638 g of magnesium sulfate, and 41.65 g of aluminum sulfate to make 1 liter of mother solution; weigh 1.564 g of EDTA and 1.168 g of ferric sulfate to make 1 liter of mother solution; weigh 0.4178 g of boric acid, 0.2933 g of zinc sulfate, 0.0648 g of copper sulfate, 0.1690 g of manganese sulfate, and 0.0823 g of sodium molybdate to make 1 liter of mother solution, and store in a refrigerator at 4°C; the final formula of the nutrient solution is a final ratio of macroelements: iron salts: trace elements of 4:1:1. Use purified water as the solvent and add a small amount of sodium hydroxide solution to make the pH value of the final nutrient solution between 4.5 and 5.5;

[0074] 6) Preparation of growth regulator B: Weigh 5 mg of naphthylacetic acid and 10 mg of indolebutyric acid respectively, mix them and dissolve them in purified water to make the final concentrations 0.5 mg / L and 1 mg / L respectively;

[0075] 7) Preparation of growth regulator C: Weigh 40 mg of 6-benzylaminopurine and 2 mg of indolebutyric acid, mix them and dissolve them in purified water to make the final concentrations 4 mg / L and 0.2 mg / L respectively;

[0076] 8) Add nutrient solution A to the hydroponic box, place the cuttage cuttings into the hydroponic box, ensure that each cutting can be exposed to the nutrient solution, and culture for 7 consecutive days; replace with new nutrient solution A, add growth regulator B, and continue to culture until new roots grow, during which the culture solution is replaced every 7 days;

[0077] 7) Germination: After new roots grow out, growth regulator C can be added to nutrient solution A. New buds will begin to germinate after about a week;

[0078] 8) Machine picking: When the young spikelets reach one bud and one leaf or one bud and two leaves, use a handheld rechargeable small tea picking machine to harvest the new shoots;

[0079] 9) Re-germination: After the new shoots are harvested, replace with new nutrient solution A. After 2 weeks, add growth regulator C to the nutrient solution. After about 1 week, a new round of new shoots begin to sprout. When they reach the harvesting standard, use a tea picking machine to harvest the new shoots.

[0080] 10) Cultivation room conditions: The temperature of the glass greenhouse is controlled at around 20-25°C, and the light source is natural light.

[0081] The results showed that the germination rate and germination time of tea tree cuttings after adding nutrient solution and growth regulator were significantly higher than those of the control ( Figure 4 ), the germination rate of the treatment group was high, and the new shoots were neat and tidy, so they could be harvested with a small tea picking machine. In addition, the time when the new shoots of the treatment group reached the standard of one bud and one leaf was about 2 weeks earlier than that of the control group ( Figure 5 ). This not only allows the new shoots of tea trees to be harvested in advance, but also saves time, effort and labor, and multiple rounds of harvesting can be achieved by replacing the nutrient solution.

[0082] Therefore, the nutrient solution proposed by the present invention can meet the basic conditions for the growth of tea seedlings, the growth regulator B can promote the rapid rooting of tea spikelets, and the growth regulator C can stimulate the rapid germination of tea seedlings. Compared with the traditional hydroponic method, the present invention can not only significantly improve the rooting rate of tea spikelets and promote the early germination of tea spikelets, but also use this hydroponic method to achieve machine picking of tea shoots, providing a good solution for the difficulty of machine picking of famous and high-quality green tea, and also laying a certain foundation for the future full mechanization (seedling-picking-processing) production of tea.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention are included in the protection scope of the present invention.

Claims

1. A nutrient solution A for indoor tea tree cutting hydroponics, characterized in that The nutrient solution A contains the following nutrients: (1) macroelements: 1 mmol / L nitrogen, 0.05 mmol / L phosphorus, 0.35 mmol / L potassium, 0.395 mmol / L calcium, 0.21 mmol / L magnesium, 0.25 mmol / L aluminum; (2) iron salts: 2.1 μmol / L iron, 2.1 μmol / L sodium; (3) trace elements: 3.33 μmol / L boron, 0.51 μmol / L zinc, 0.5 μmol / L manganese, 0.17 μmol / L molybdenum, 0.13 μmol / L copper.

2. A growth regulator B for indoor tea tree cutting rooting by hydroponic cuttings, characterized in that The growth regulator B comprises the following nutrients: 0.5 mg / L NAA+1 mg / L IBA, wherein NAA is naphthylacetic acid and IBA is indolebutyric acid.

3. A growth regulator C for indoor tea tree cuttings hydroponic germination, characterized in that The growth regulator C comprises the following nutrients: 4 mg / L 6-BA+0.2 mg / L IBA, wherein 6-BA is 6-benzylaminopurine and IBA is indolebutyric acid.

4. A method suitable for indoor tea tree cuttings hydroponic rooting, germination and mechanical harvesting, characterized in that The steps include: 1) Branch preparation: Collect the semi-lignified new shoots of the tea tree of the year, soak them in a carbendazim solution, and then wash off the surface soil and other impurities with clean water; 2) Cutting pruning: Use pruning shears to cut off the cuttings on the new shoots one by one. Each cutting carries a mature leaf. Remove the cuttings with pests and diseases. Then cut off 2 / 3 of the mature leaves on the cuttings, and select cuttings of qualified size for cuttings; 3) Cutting board production: Use thickened EPE pearl cotton foam board as the cutting board, and draw the spacing of the cuttings on the cutting board; 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box; 5) Prepare nutrient solution A, growth regulator B and growth regulator C; 6) Add nutrient solution A to the hydroponic box, place the cuttage cuttings into the hydroponic box, ensure that each cutting can be exposed to the nutrient solution, and replace with new nutrient solution A after 7 consecutive days of cultivation, and add growth regulator B, and continue to cultivate until new roots grow, during which the culture solution is replaced every 7 days; 7) Germination: After new roots grow out, growth regulator C can be added to nutrient solution A. New buds will begin to sprout after about a week. If buds are to sprout first, add growth regulator C first in the above steps. 8) Machine picking: When the young spikelets reach one bud and one leaf or one bud and two leaves, use a handheld rechargeable small tea picking machine to harvest the new shoots; 9) Re-germination: After the new shoots are harvested, replace with new nutrient solution A. After 2 weeks, add growth regulator C to the nutrient solution. After about 1 week, the new shoots begin to sprout. When they reach the harvesting standard, use a tea picking machine to harvest the new shoots.

5. The method according to claim 4, characterized in that The steps include: 1) Branch preparation: Collect the semi-lignified new shoots of tea trees of the current year, soak them in 1% carbendazim solution for 2 hours, and then wash off the surface soil and other impurities with clean water; 2) Spike pruning: Use pruning shears to cut off the spikes on the new shoots one by one. The length of the spikes is about 4 cm. Each spike carries a mature leaf. Remove the spikes with pests and diseases, and then cut off 2 / 3 of the mature leaves on the spikes. Select spikes of qualified size for cuttings; 3) Cutting board production: Use thickened EPE pearl cotton foam board as the cutting board, cut the size to length × width × thickness = 45cm × 30cm × 1.5cm, use a black silk marker to draw the cutting row spacing of 3 × 3cm on the cutting board, and the number of spikes set on each cutting board is 140; 4) Preparation of hydroponic box: Use thickened plastic turnover box as hydroponic box, the box size is 45×30×15cm; 5) Prepare nutrient solution A, growth regulator B and growth regulator C; 6) Add nutrient solution A to the hydroponic box, place the cuttage cuttings into the hydroponic box, ensure that each cutting can be exposed to the nutrient solution, and replace with new nutrient solution A after 7 consecutive days of cultivation, and add growth regulator B, and continue to cultivate until new roots grow, during which the culture solution is replaced every 7 days; 7) Germination: After new roots grow out, growth regulator C can be added to nutrient solution A. New buds will begin to sprout after about a week. If buds are to sprout first, add growth regulator C first in the above steps. 8) Machine picking: When the young spikelets reach one bud and one leaf or one bud and two leaves, use a handheld rechargeable small tea picking machine to harvest the new shoots; 9) Re-germination: After the new shoots are harvested, replace with new nutrient solution A. After 2 weeks, add growth regulator C to the nutrient solution. After about 1 week, the new shoots begin to sprout. When they reach the harvesting standard, use a tea picking machine to harvest the new shoots.

6. The method according to claim 4 or 5, characterized in that The preparation method of the nutrient solution A is as follows: weigh the macro-elements ammonium sulfate, calcium nitrate tetrahydrate, potassium dihydrogen phosphate, potassium sulfate, calcium chloride, magnesium sulfate, and aluminum sulfate respectively in proportion to prepare 1 liter of mother liquor; weigh the iron salt EDTA and iron sulfate in proportion to mix and prepare 1 liter of mother liquor; weigh the trace elements boric acid, zinc sulfate, copper sulfate, manganese sulfate, and sodium molybdate in proportion to mix and prepare 1 liter of mother liquor, and store it in a refrigerator at 4°C; the nutrient solution formula is a ratio of macro-elements: iron salts: trace elements of 4:1:1, pure water is used as a solvent, and a small amount of sodium hydroxide solution is added to make the pH value of the final nutrient solution between 4.5 and 5.

5.

7. The method according to claim 4 or 5, characterized in that The preparation method of the growth regulator B is as follows: naphthylacetic acid and indolebutyric acid are weighed separately, mixed and dissolved in purified water, and the final concentrations thereof are 0.5 mg / L and 1 mg / L respectively.

8. The method according to claim 4 or 5, characterized in that The preparation method of the growth regulator C is as follows: 6-benzylaminopurine and indolebutyric acid are weighed separately, mixed and dissolved in purified water, and the final concentrations thereof are 4 mg / L and 0.2 mg / L respectively.

9. The method according to claim 4 or 5, characterized in that The culture room is a glass greenhouse with a temperature controlled at around 20-25°C and natural light as the light source.

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