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Application of fulvic acid in prevention and treatment of postharvest grey mould of grape berries

A technology for fulvic acid and botrytis cinerea, which is applied in the application field of fulvic acid in the prevention and treatment of botrytis cinerea after grape harvest, can solve the problems of restricting the development of grape industry, fruit drop and fruit rot, fruit quality change, etc., and achieves beneficial results. On human health, enhance antibacterial effect, reduce the effect of toxic and side effects

Inactive Publication Date: 2018-06-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gray mold caused by Botrytis cinerea is an important plant disease, which mainly harms grape flower spikes and fruits on grapes, causing flower drop, fruit drop and fruit rot, which seriously affects the post-harvest storage of grapes. luck, limiting the development of the grape industry
[0003] At present, in the post-harvest treatment of grapes, mainly through SO 2 Fumigation is used to achieve the purpose of preventing and controlling pests and diseases, but the resulting changes in fruit quality and SO 2 The hazards of residues to human body and other issues cannot be ignored, so it is urgent to explore new alternative methods to control grape postharvest botrytis

Method used

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  • Application of fulvic acid in prevention and treatment of postharvest grey mould of grape berries
  • Application of fulvic acid in prevention and treatment of postharvest grey mould of grape berries
  • Application of fulvic acid in prevention and treatment of postharvest grey mould of grape berries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the control effect experiment of fulvic acid to postharvest grape gray mold

[0023] Test materials: the test grape variety "mare milk", purchased from the market.

[0024] The single-grain grape fruit was manually separated, leaving the fruit pedicle, and healthy fruit grains with the same size, shape, and maturity were selected and sterilized in 2% sodium hypochlorite solution for 2 minutes, then rinsed twice with sterile water, dried and grouped.

[0025] (1) Soak the grape fruits in different concentrations of fulvic acid solutions for 10 minutes, set 3 replicates for each concentration, and treat 30 fruits for each replicate.

[0026] (2) After drying the fruits naturally, place all the fruits at 22°C for 24 hours.

[0027] (3) Cultivate Botrytis cinerea on PDA medium for 5 days, then add sterile water to the plate for washing and shaking, and prepare spore suspension (concentration: 3 × 10 4 individual / mL).

[0028] (4) The fruit is artificially s...

Embodiment 2

[0035] Embodiment 2, the influence of fulvic acid on Botrytis cinerea mycelia growth

[0036] Fulvic acid was dissolved in sterile deionized water, and then added to PDA medium to form a medium containing fulvic acid, and the inhibitory effect of different concentrations of fulvic acid on the growth of pathogenic fungi was determined by the mycelial growth rate method. Specific steps are as follows:

[0037] Use sterile deionized water as a solvent to dissolve fulvic acid to form a mother solution, and then accurately add a certain amount of fulvic acid solution to the melted PDA medium at 50°C and mix evenly, so that the final concentrations of fulvic acid in the medium are respectively Reach 0.1, 0.5, 1, 10 and 50mg / mL, each concentration treatment set 3 replicates, with the same volume of sterile deionized water added as a control. After the tested Botrytis cinerea was cultured on the PDA medium for 5 days, use a sterilized puncher to get bacteria cakes (d=6mm) with the sa...

Embodiment 3

[0039] Embodiment 3, the influence of fulvic acid on grape fruit polyphenol content

[0040] Fulvic acid was dissolved in sterile deionized water to a final concentration of 10 mg / mL. Grape fruits were soaked in the fulvic acid solution for 10 minutes and then taken out to dry. Grape fruits were treated with the same volume of deionized water as the experimental control. After placing at 22°C for 24h, inoculate the botrytis cinerea spore suspension with reference to the method of Example 1, then place it under dark conditions at 22°C for cultivation, and regularly sample the grape fruits of each treatment every 12h to prepare for polyphenols Extraction determination of substance content.

[0041] The extraction and determination method of the total polyphenol content in grape peels is as follows: artificially separate the peels from the sampled grape fruit, blot the water with filter paper, quickly freeze the peels in liquid nitrogen and grind the peels to powder, add 8mL of 8...

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Abstract

The invention discloses the application of fulvic acid in prevention and treatment of postharvest grey mould of grape berries. During prevention and treatment of the grey mould of the grape berries, afulvic acid aqueous solution with the mass-volume concentration ranging from 0 to 20 mg / mL, but not zero, is adopted, specifically, the content of polyphenol in grapes is increased. A fulvic acid substance adopted in the application can be used for preventing and treating the postharvest grey mould of the grapes. The fulvic acid substance can be independently used or cooperated with other activesubstances for use, so as to enhance the antibacterial effect or reduce the toxic and side effects of the fulvic acid substance; fulvic acid has the characteristics of green color and safety, is beneficial for the health of a human body, meets a requirement for food safety, and achieves effects of preventing and treating the grey mould and preserving the freshness of fruits.

Description

technical field [0001] The invention relates to the application of fulvic acid in preventing and treating postharvest gray mold of grapes, and belongs to the field of biotechnology. Background technique [0002] Grape is an important economic crop. Its uses involve winemaking, fresh food and dried fruit, etc. It is closely related to the development of the national economy and personal health. However, gray mold caused by Botrytis cinerea is an important plant disease, which mainly harms grape flower spikes and fruits on grapes, causing flower drop, fruit drop and fruit rot, which seriously affects the post-harvest storage of grapes. This has limited the development of the grape industry. [0003] At present, in the post-harvest treatment of grapes, mainly through SO 2 Fumigation is used to achieve the purpose of preventing and controlling pests and diseases, but the resulting changes in fruit quality and SO 2 The hazards of residues to human health cannot be ignored, so ...

Claims

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Application Information

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IPC IPC(8): A01N61/00A01P3/00A01P21/00
CPCA01N61/00
Inventor 高灵旺徐丹丹习平根于戈姜子德王琦李文明香梅赵粮
Owner CHINA AGRI UNIV
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