47 results about "Botrytis cinerea" patented technology

The present application relates to the technical field of pesticide chemistry and medicinal chemistry, and particularly relates to a substituted pyrazole-containing ortho-hydroxy aryl formamide derivative, a preparation method and application thereof. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative is prepared from a 5-amino pyrazole intermediate (I) and a different substituted ortho-hydroxy aryl formic acid (II) as raw materials through an amide condensation reaction. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative prepared by the present application has a broad-spectrum inhibitory activity on plant pathogenic fungi, and particularly has excellent inhibitory activity on Valsa mali, Sclerotinia sclerotiorum and Botrytis cinerea, and is expected to be developed into a new type of green fungicide with high efficiency, safety, economy and environmental friendliness.

This invention belongs to the field of agricultural biotechnology, and specifically relates to a strain of *Dictyophora indica* Phd01, its inoculant, and its application. *Dictyophora indica* Phd01 is classified and named... Phallus dongsun The strain was deposited on January 23, 2026, at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2026233, located at Wuhan University, Wuhan, China. The fermentation broth of this strain significantly inhibited the mycelial growth of *Botrytis cinerea* and *Anthracnose spp.*; it showed a relative control efficacy of 42.31% against gray mold in peppers and enhanced the oxygen free radical scavenging capacity of pepper fruits, effectively extending the storage time of pepper fruits; the relative control efficacy against anthracnose in strawberries reached 31.03–40.23%, and it prolonged the time of strawberry disease onset. This indicates that the fermentation broth of *Dendrobium nobile* Phd01 can significantly inhibit strawberry and pepper rot, thereby extending shelf life, and has the potential to be developed into an environmentally friendly biological agent.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

The present application belongs to the technical field of biological control, and particularly relates to a microbial agent for preventing and treating gray mold of crops, and a preparation method and application thereof. The present application provides a microbial agent for preventing and treating gray mold of crops, which comprises a microbial strain and / or a fermentation broth of the microbial strain; the microbial strain comprises one or more of Bacillus subtilis JD-L9, Pseudomonas lundensis DS-PZ-P18 and Pseudomonas chlororaphis HQ-CZ-P12; wherein the Pseudomonas chlororaphis HQ-CZ-P12, the Pseudomonas lundensis DS-PZ-P18 and the Bacillus subtilis JD-L9 have a good inhibition rate on the pathogenic fungus of gray mold, and after being compounded into a compound microbial agent, the compound microbial agent is sprayed on seedlings of medicinal materials, so that the incidence of gray mold during seedling raising is significantly reduced, pesticide residues of the medicinal materials are reduced, and the safety of the medicinal materials is improved.

A transcription factor for regulating resistance of kiwifruit to botrytis blight AcWRKY84 The application relates to a gene, and after the expression of the gene is silenced, the resistance of kiwifruit to botrytis blight is significantly reduced. AcWRKY84 The application clones the gene and proves the role of the gene in resisting botrytis blight in kiwifruit, and specifically, after the experimental group is inoculated with the virus, the disease symptoms of the experimental group are more serious, the infected area is obviously increased, and the SOD, POD and CAT enzyme activities in the fruit are significantly reduced compared with those of the control group, so that sufficient gene resources can be provided for a botrytis blight resistance gene library, and the possibility of realizing successful breeding of a high botrytis blight resistance variety is greatly increased.

This invention discloses the tea plant miRNA miR408-p5 and its application and method in improving plant disease resistance. By constructing a silencing expression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, exhibiting strong fungal inhibitory activity against *Botrytis cinerea*. Conversely, by constructing an overexpression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, showing significantly weakened resistance to *Botrytis cinerea*. Therefore, silencing miR408-p5 expression can improve plant disease resistance, and introducing the miR408-p5 silencing expression vector into plants such as tobacco, tea, or vegetables to enhance plant disease resistance has broad market application prospects.

This invention discloses coumarin Schiff base derivatives. These derivatives are based on coumarin, and a series of coumarin derivatives with Schiff base structures are introduced through a reaction. These compounds exhibit good antifungal activity against *Botrytis cinerea*, *Alternaria alternata*, *Fusarium oxysporum*, and *Alternaria alternata*, opening up new avenues for the control of various plant fungal diseases and possessing broad development potential and promising application prospects. Furthermore, in vitro antioxidant experiments on these Schiff base compounds showed that they exhibit excellent scavenging abilities against DPPH radicals, ABTS radicals, and hydroxyl radicals, with antioxidant performance comparable to the positive control ascorbic acid, demonstrating their potential as novel antioxidants.

This invention relates to the fields of pesticide chemistry and medicinal chemistry, and in particular to a coumarin derivative containing a substituted phenyl ether structure, its preparation method, and its applications. This invention organically combines a coumarin structure with a substituted phenyl ether fragment to synthesize a novel class of coumarin derivatives containing a substituted phenyl ether structure. These compounds exhibit excellent antifungal activity and broad-spectrum activity, demonstrating superior inhibitory activity against *Agropyron cristatum*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, and *Tricholoma matsutake*. This invention provides a foundation for the development of novel fungicides using coumarin derivatives containing substituted phenyl ether structures as active ingredients.

The present application relates to a fungicide for crops, and in particular to a composite fungicide containing plant extracts, a preparation process and application thereof.The composite fungicide comprises plant extracts and a fungicide preparation.The present application selects propineb and fluazinam in a weight ratio of 1:9-9:1 as the fungicide preparation, which can effectively prevent and control grape botrytis cinerea.In addition, plant extracts of chebula, oregano, thyme and sophora flavescens are used to optimize the performance of the extracts in inhibiting grape botrytis cinerea, and the composite fungicide containing plant extracts has good application prospect.

The application provides a strain of Candida zeylanoides, and applications of the strain in inhibiting Botrytis cinerea and in wine brewing, the Candida zeylanoides is numbered SIVE8902, has been preserved in the China Center for Type Culture Collection on July 22, 2024, the preservation number is CCTCC NO:M 20241637, and the Latin name is Starmerella bacillaris The strain has a strong inhibiting effect on Botrytis cinerea in grape fresh fruits and vineyards, can reduce the growth and reproduction of Botrytis cinerea in the fruits, and meanwhile, compared with single fermentation of Saccharomyces cerevisiae, the sequential fermentation of the Candida zeylanoides and Saccharomyces cerevisiae adds ethylguaiacol and 4-ethyl-2-methoxyphenol, adds spice and honey flavors to the wine, greatly improves the total amount of volatile phenolic compounds in the wine, and improves the wine quality.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

This invention discloses a berberine hydrochloride-resveratrol self-assembled nanopesticide, its preparation method, and its application. The preparation method involves dissolving berberine hydrochloride and resveratrol separately in an organic solvent, mixing them in a molar ratio of 3:1–1:3, adding the mixture to deionized water, and then adding an alkaline solution; the mixture then self-assembles to obtain nanoparticles. Based on the principle of supramolecular construction, this invention utilizes the high compatibility of berberine hydrochloride and resveratrol in charge distribution and spatial geometry. Through multiple intermolecular forces such as electrostatic attraction, π-π stacking, and hydrogen bonding, the precise recognition and directional assembly of the two active molecules in the aqueous phase are achieved. This nanopesticide requires no additional synthetic adjuvants or nanocarriers, achieving confined enrichment of the active ingredients at the nanoscale, resulting in a significant synergistic effect. This nanopesticide significantly enhances the inhibitory activity against various pathogens such as *Botrytis cinerea*, *Fusarium oxysporum*, *Phytophthora capsici*, and *Rhizoctonia solani*, and exhibits excellent environmental biosafety.

This invention relates to a Bacillus subtilis ( Bacillus subtle Y32, with accession number CGMCC No. 34819, is a strain that produces heat-resistant and highly stable xylanase, as well as various hydrolases such as β-mannanase and protease. It also produces extracellular polysaccharides, inhibits Botrytis cinerea, and is acid-resistant, resistant to porcine bile salts, and resistant to 10% NaCl. The optimal temperature for the xylanase produced by this strain is 55℃, and the optimal pH is 6.0. Under these conditions, the half-life is 12.5 h, and the relative enzyme activity remains above 80% after 3 h of incubation, demonstrating good thermal stability. This strain can also increase the yield of cucumbers and Pinellia ternata, and increase the egg production rate of 70-week-old Hy-Line Brown hens from 70.4% to 90.1%. This strain has promising applications in agriculture, food processing, and cosmetics production.

The application relates to the field of postharvest preservation of flowers and cut flower vase planting and maintenance technology, in particular to a composite preservative liquid for prolonging the preservation period of cut rhododendron flowers and a preparation method thereof. The method comprises the following steps: taking part of deionized water, sequentially adding trehalose, sucrose and potassium nitrate, stirring and dissolving to obtain a sugar salt water phase; taking part of deionized water, adding citric acid and stirring and dissolving, then adding ferrous sulfate, stirring in the dark to obtain a ferrous salt acidification pre-solution; slowly adding the ferrous salt acidification pre-solution into the sugar salt water phase, mixing under stirring to obtain a composite water phase; dissolving gibberellin in ethanol to prepare a gibberellin mother liquor, and adding the gibberellin mother liquor into the composite water phase; adding diluted sec-butylamine into the obtained solution, stirring and mixing; adding the remaining deionized water to constant volume; filtering and sterilizing the adjusted solution, filling and sealing to obtain the composite preservative liquid. The application can inhibit the reproduction of botrytis cinerea and maintain the good state of cut flowers.

The present invention relates to a strain of the fungus Botrytis cinerea that exhibits a hypovirulent phenotype due to infection with at least the mycovirus Botrytis cinerea endornavirus 2 (BcEV2). In a preferred embodiment, the strain is co-infected with BcEV2 and the mycovirus Botrytis cinerea ourmia-like virus 2 (BcOLV2), resulting in a hypovirulent phenotype that is dependent on the co-presence of both viruses, and is registered under accession number CECT 21321. The invention also describes compositions comprising this strain and its use as a biological control agent to prevent or treat gray mold disease in a wide variety of crops of agricultural interest. The strain of the invention acts by transmitting the mycovirus combination to pathogenic strains of B. cinerea in the field, attenuating their virulence and thus providing an effective and sustainable alternative to chemical fungicides.

This invention belongs to the field of microbiology, specifically relating to a non-pathogenic strain of *Botrytis cinerea* IBc-374 and its applications. This invention provides a strain of *Botrytis cinerea* IBc-374, which exhibits slow mycelial growth, weak sporulation and sclerotinia production, and completely lacks pathogenicity. *Botrytis cinerea* IBc-374 can delay the occurrence of sclerotinia rot, providing significant protection to crops, improving field prevention of sclerotinia rot, and also significantly promoting crop growth. Therefore, *Botrytis cinerea* IBc-374 can be used as a biocontrol and growth-promoting strain for the control of sclerotinia rot and for increasing crop yield.

The application discloses tea tree miRNA PC-5p-680261 and application and methods thereof in improving plant disease resistance. By constructing a silencing expression vector of the miRNA PC-5p-680261, the miRNA PC-5p-680261 is introduced into tobacco, and a transgenic tobacco plant is obtained through stable genetic transformation, and the transgenic tobacco plant shows strong fungal inhibition activity to inoculation of Botrytis cinerea; by constructing an overexpression vector of the miRNA PC-5p-680261, the miRNA PC-5p-680261 is introduced into tobacco, and a transgenic tobacco plant is obtained through stable genetic transformation, and the disease resistance of the tobacco plant to the Botrytis cinerea is significantly weakened. Therefore, silencing the expression of the miRNA PC-5p-680261 has the effect of improving the disease resistance of the plant, and the silencing expression vector of the miRNA PC-5p-680261 is introduced into tobacco, tea tree or vegetables and other plants to improve the disease resistance of the plant, so that the application has a wide market application prospect.

This invention belongs to the field of pesticide technology, and relates to oxime derivatives containing a 3,4-dihydroisoquinoline structure, their preparation methods, and applications. This invention synthesizes a class of oxime derivatives containing a 3,4-dihydroisoquinoline structure by introducing an oxime functional group into the 3,4-dihydroisoquinoline structure. These oxime derivatives and their hydrochlorides exhibit inhibitory effects on various plant pathogens, especially on Ascomycota and Deuteromycota pathogenic fungi, such as *Pseudomonas aeruginosa*, *Pseudomonas macrantha*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Fusarium graminearum*, and *Bacillus thuringiensis*, demonstrating excellent inhibitory activity. Therefore, oxime derivatives containing a 3,4-dihydroisoquinoline structure hold promise for further development into highly efficient, safe, economical, and environmentally friendly green fungicides.

The application discloses a diflunisal derivative, a preparation method and application thereof, and belongs to the technical field of agricultural fungicides. The preparation method comprises the following steps: mixing 2', 4'-difluoro-4-hydroxy-[1, 1'-biphenyl]-3-carboxylic acid, a condensing agent and an organic base in an ice bath and an inert organic solvent, and activating for 10-30 minutes; then adding an amine compound with a general formula of R-NH2, and stirring and reacting at room temperature for 20-28 hours; after the reaction is completed, extraction, washing, drying, concentration and column chromatography purification are performed, so that the target diflunisal derivative is obtained. The compound disclosed by the application has a novel structure, shows excellent inhibitory activity on cucumber botrytis cinerea, rice pellicularia sasakii, brassica rapa sclerotium, wheat fusarium graminearum and other plant pathogenic fungi, can be used as an active ingredient for preparing an agricultural fungicide or a fungicide composition, and provides a new lead structure for developing a new succinate dehydrogenase inhibitor fungicide.

The invention relates to the technical field of agriculture, and particularly discloses a comprehensive pest and disease damage prevention and control method combining winter peach fruit bagging with trapping and killing technology, which comprises the following steps: in a winter peach physiological fruit dropping period, suspending insect sex pheromone trappers in an orchard, and monitoring the adult sexual period of peach fruit moths and oriental fruit moths; s2, according to the monitoring result of the step S1, all fruits are uniformly bagged before the initial emergence period of the target pest adults and after fruit setting is completed; physical bagging and various trapping and killing technologies are systematically integrated in time sequence and space, pest situation monitoring is used as a guide, the optimal window period of bagging is determined, physical isolation measures are made to play the maximum effect, a composite trapping and killing system is deployed subsequently, space differentiation configuration is conducted according to ecological habits and activity rules of different pests, and the pest trapping and killing effect is improved. Accurate coverage of the trapping and killing range and the target object is achieved.

This invention belongs to the field of biological control of plant diseases, specifically relating to a virulent strain of *Botrytis cinerea* SdBd-6 and its applications. The virulent strain of *Botrytis cinerea* SdBd-6 is classified and named... Botryosphaeria dothidea The attenuated strain SdBd-6 of Staphylococcus aureus was deposited at the Guangdong Provincial Center for Microbial Culture Collection on January 14, 2026, with accession number GDMCC: 67656. Walsa money The growth of the fungus has a certain inhibitory function. At the same time, after 10 generations of continuous inoculation, the virus contained in the attenuated strain SdBd-6 of *Botrytis cinerea* was not lost, and its growth characteristics and pathogenicity remained attenuated strain characteristics. This provides materials and lays the foundation for the green control of plant fungal diseases caused by the pathogenic fungus *Botrytis cinerea*.

This invention discloses a 1-(2-aminobenzyl)-3-arylurea compound, its preparation method, and its uses. This type of compound is synthesized from o-aminobenzylamine and aryl isocyanate compounds as raw materials, using triethylamine as an acid-binding agent and dichloromethane as a solvent via a condensation reaction in an ice-water bath. The raw materials used in the synthesis of this type of compound are inexpensive and readily available, and the synthesis method is simple. It exhibits good inhibitory effects on crop pathogens, particularly showing significant inhibitory activity against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, Carbendazim, Botrytis cinerea, and Sheath blight.

The application discloses a melatonin derivative and application of the melatonin derivative in prevention and treatment of citrus penicillium disease. The compound has a structural formula shown in the following formula (I). Formula (I); the application has very strong inhibitory activity on pathogenicity of penicillium italicum and has good broad-spectrum properties. The application has inhibitory effects on fusarium graminearum, botrytis cinerea, sclerotinia sclerotiorum, cladosporium cladosporioides, pellicularia sasakii, lysobacter lactarius, fusarium oxysporum and fusarium oxysporum, and has great application value.

This invention discloses the use of a quinazoline-diaryl ether derivative in inhibiting and / or controlling pathogenic microorganisms, relating to the field of medicinal chemistry. It solves the technical problems of existing pesticides being highly toxic, prone to resistance, and lacking a suitable combination of quinazoline and diaryl ether compounds. The quinazoline-diaryl ether derivative of this invention exhibits potential inhibitory effects on plant bacteria such as *Rhizoctonia solani*, *Rhizoctonia solani*, and *Rhizoctonia solani*, as well as plant fungi such as *Rhizoctonia solani*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Bacillus oryzae*, and *Fusarium graminearum*, and agricultural pathogenic nematodes, demonstrating antibacterial activity and a broad spectrum.

This invention discloses a *Trichoderma echinococcus* strain BBR-A and its application in the control of fungal diseases in blueberries, belonging to the field of biological control technology for blueberry fungal diseases. A *Trichoderma echinococcus* strain BBR-A was isolated from the rhizosphere soil of healthy blueberries and deposited at the China Center for Type Culture Collection (CCTCC) on June 4, 2025, with accession number CCTCC NO: M20251239. The *Trichoderma echinococcus* strain BBR-A of this invention significantly inhibits *Botrytis cinerea*, *Alternaria alternata*, and *Fusarium oxysporum*. Microscopic and electron microscopic examination revealed that *Trichoderma echinococcus* hyphae exhibited hyperparasitic phenomena such as entanglement, penetration, and dissolution upon contact with the hyphae of these three pathogens. The incidence of disease in blueberry roots and leaves treated with *Trichoderma echinococcus* was significantly reduced. These experimental results demonstrate that *Trichoderma echinococcus* BBR-A has a significant inhibitory effect on the growth and pathogenicity of blueberry fungal pathogens and can be used as a biological control agent for the control of blueberry fungal diseases, showing good application prospects.

This invention discloses a method for detecting resistance to methoxyacrylate (QoIs) fungicides caused by single-base mutations in *Botrytis cinerea*, the causal agent of grape gray mold. This invention is based on the RPA-CRISPR / Cas12a system and designs a method targeting the fungicide. cytb This invention relates to a rapid, simple, and specific method for detecting resistance to Botrytis cinerea using a gene containing specific primer pairs with mutation sites and specific crRNA for activating Cas12a cleavage activity, combined with a fluorescent reporter probe. The method allows for real-time field detection without complex instruments, demonstrating promising application prospects and providing guidance for the healthy development of the grape industry and the scientific prevention of Botrytis cinerea.