233 results about "Botrytis cinerea" patented technology

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.

The invention relates to the technical field of biological prevention and control, and discloses application of a bacillus velezensis P15 fungicide in preservation of harvested strawberries, and the application comprises the step of applying the bacillus velezensis P15 fungicide to the harvested strawberries so as to prevent and control strawberry postharvest diseases caused by pathogenic fungi. The preservation number of the bacillus velezensis P15 is CGMCC (China General Microbiological Culture Collection Center) NO.31995. The bacillus velezensis P15 microbial inoculum is used for preservation of picked strawberries, can efficiently inhibit more than 90% of picked pathogenic bacteria such as botrytis cinerea and the like, has a control effect superior to that of prochloraz, and does not enable the pathogenic bacteria to resist drugs after being used for a long time; the strawberry preservative is harmless to human, animals, plants and the environment, has no microbial agent residue, can be used for fresh keeping of green and organic strawberries, solves the food safety problem of chemical preservative residue, and improves the acceptability of consumers.

The invention discloses a sodium alginate-quaternary ammonium salt cationic surfactant pesticide adjuvant as well as a preparation method and application thereof, and belongs to the technical field of pesticide adjuvants. The invention discloses a sodium alginate-quaternary ammonium salt cationic surface active agent pesticide adjuvant. The sodium alginate-quaternary ammonium salt cationic surface active agent pesticide adjuvant is prepared from sodium alginate and a quaternary ammonium salt cationic surface active agent. The quaternary ammonium salt cationic surface active agent and the sodium alginate can form the pesticide adjuvant with uniform size through simple electrostatic attraction and hydrophobic interaction between surface active agent carbon chains. Tebuconazole added with the pesticide adjuvant has a certain synergistic effect on the antibacterial activity of alternaria alternata, fusarium oxysporum and botrytis cinerea. And the auxiliary agent is non-toxic to the germination of plants, and accords with the development trend of green pesticides.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The present application relates to the technical field of pesticide chemistry and medicinal chemistry, and particularly relates to a substituted pyrazole-containing ortho-hydroxy aryl formamide derivative, a preparation method and application thereof. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative is prepared from a 5-amino pyrazole intermediate (I) and a different substituted ortho-hydroxy aryl formic acid (II) as raw materials through an amide condensation reaction. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative prepared by the present application has a broad-spectrum inhibitory activity on plant pathogenic fungi, and particularly has excellent inhibitory activity on Valsa mali, Sclerotinia sclerotiorum and Botrytis cinerea, and is expected to be developed into a new type of green fungicide with high efficiency, safety, economy and environmental friendliness.

The invention discloses a transcription factor AcWRKY84 gene for regulating and controlling gray mold resistance of kiwi fruits. After the expression of the gene is silenced, the gray mold resistance of kiwi fruits can be remarkably reduced. The AcWRKY84 gene is cloned, and the anti-gray mold effect of the AcWRKY84 gene in kiwi fruits is proved, specifically, after an experimental group is inoculated with viruses, compared with a control group, the disease symptoms of the fruits of the experimental group are aggravated, the infected area is obviously enlarged, and the enzyme activities of SOD, POD and CAT in the fruits are obviously reduced compared with the control group; sufficient gene resources can be provided for a kiwi fruit gray mold resistance gene pool, so that the possibility of successfully breeding high-gray mold-resistance varieties subsequently is greatly increased.

The invention relates to the technical field of fungus culture, and discloses a culture medium for inducing Stemphylium solani to produce spores, a preparation method and application, the preparation raw materials of the culture medium comprise a tomato extract, a carrot extract and agar, the total mass of the tomato extract and the carrot extract is 50g / L to 200g / L, and the content of the tomato extract is not less than 50g / L. According to the culture medium for inducing the Stemphylium solani to produce the spores, the preparation method and the application, the tomato extract and the carrot extract in a specific ratio are combined, so that optimal nutrition and environmental conditions are provided for the Stemphylium solani to produce the spores. Experimental data show that the sporulation quantity of the culture medium is remarkably higher than that of a conventional culture medium (such as CA, V8, PDA and the like), the technical problem that an existing solid culture medium is unstable in sporulation or does not produce sporulation is solved, the selected core raw materials tomato and carrot are common fruits and vegetables, low in cost and easy to obtain, expensive or special chemical reagents are avoided, and the production cost is reduced. And the research and development and application cost is greatly reduced.

This invention discloses the application of the FAD2 gene of *Botrytis cinerea* in regulating the growth, pathogenicity, and stress resistance of *B. cinerea*. The FAD2 gene of this invention is derived from *B. cinerea*. By constructing a knockout gene fragment of the FAD2 gene and introducing it into the protoplasts of *B. cinerea*, a knockout mutant of the FAD2 gene was obtained. Studies have found that the FAD2 gene plays an important role in regulating the growth of *B. cinerea*, influencing its ability to withstand high salinity and osmotic stress, and significantly reducing the pathogenicity of *B. cinerea* mycelium to tomato fruits and leaves. Therefore, FAD2 can serve as a key gene for studying the pathogenic mechanism of the disease and can be used as a target for fungicides. This can be used to develop fungicides to control *B. cinerea* or for gene function research on the pathogen, contributing to the breeding of new plant disease-resistant varieties and showing broad application prospects in plant fungal disease research.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

This invention belongs to the field of agricultural biotechnology, and specifically relates to a strain of *Dictyophora indica* Phd01, its inoculant, and its application. *Dictyophora indica* Phd01 is classified and named... Phallus dongsun The strain was deposited on January 23, 2026, at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2026233, located at Wuhan University, Wuhan, China. The fermentation broth of this strain significantly inhibited the mycelial growth of *Botrytis cinerea* and *Anthracnose spp.*; it showed a relative control efficacy of 42.31% against gray mold in peppers and enhanced the oxygen free radical scavenging capacity of pepper fruits, effectively extending the storage time of pepper fruits; the relative control efficacy against anthracnose in strawberries reached 31.03–40.23%, and it prolonged the time of strawberry disease onset. This indicates that the fermentation broth of *Dendrobium nobile* Phd01 can significantly inhibit strawberry and pepper rot, thereby extending shelf life, and has the potential to be developed into an environmentally friendly biological agent.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The invention discloses a quinolinic acid diaryl ether derivative, a preparation method and application thereof and a bacteriostatic agent or bactericide, relates to the technical field of medicinal chemistry, solves the problems that existing pesticides are high in toxicity and not environmentally friendly, easily cause drug resistance of pathogenic bacteria and possibly have negative effects on the environment and an ecological system, can efficiently prevent and treat fungal diseases of plants, and also can effectively prevent and treat the pathogenic bacteria of the plants. The quinolinic acid diarylether derivative has the advantages that the quinolinic acid diarylether derivative has excellent inhibition activity on agricultural fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum and fusarium pseudograminearum, and the quinolinic acid diarylether derivative has the characteristics of simple synthesis process, cheap and easily available raw materials, good control effect, wide bactericidal spectrum and the like.

The invention belongs to the technical field of pesticide chemistry, and relates to a quinoline derivative containing a piperazine structure and a preparation method and application thereof. The invention provides a quinoline derivative containing a piperazine structure, and the quinoline derivative has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on valsa mali, sclerotinia sclerotiorum, botrytis cinerea, gaeumannomyces graminis and magnaporthe oryzae. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

This invention discloses a strain of *Botrytis cinerea* BA0616 and its application in low-purine foods, relating to the field of microbial technology. The *Botrytis cinerea* BA0616 strain of this invention has the accession number GDMCC NO: 65287. This *Botrytis cinerea* strain can use purines as a carbon source and has a strong metabolic capacity for purines, making it suitable for fermentation in low-purine foods. Applying *Botrytis cinerea* to beer production, without reducing the amount of barley malt used or adding adsorbents, strong acids, strong alkalis, or exogenous additives such as enzymes, the resulting low-purine beer, co-fermented with brewer's yeast, has rich foam and a delicate taste. The final purine content of the beer is only 8-15 mg / L, only 1 / 5-1 / 10 of that of ordinary beer, making it healthier to drink.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The invention belongs to the technical field of plant disease control, and particularly relates to application of N-hydroxypiperidine acid to improvement of postharvest disease resistance of tomato fruits. The technical problem to be solved by the invention is to provide a new choice for improving the disease resistance of the picked tomato fruits in the storage stage. The technical scheme of the invention is to provide application of N-hydroxypiperidine acid in improvement of postharvest disease resistance of tomato fruits. According to the technical scheme, the N-hydroxypiperidine acid is used, so that the resistance of the picked tomato fruits to botrytis cinerea can be effectively improved, the MDA content in the storage period of the picked fruits is reduced, the peroxidase activity and catalase activity of the fruits are improved, and the disease resistance of the picked tomato fruits is integrally and effectively improved. The method is simple and convenient to operate, low in cost, obvious in effect and good in application value.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

According to the application of the kiwi fruit transcription factor AcbZIP87 gene in gray mold resistance, after the expression of the gene is inhibited, the resistance of kiwi fruit to gray mold can be remarkably reduced. A gene coding series of the cloned AcbZIP87 gene is shown as SEQ ID NO. 1, and the effect of the AcbZIP87 gene in resisting gray mold in kiwi fruits is proved, specifically, compared with a control group after virus inoculation, the disease symptoms of obtained transgenic fruits are aggravated, the infected area is obviously enlarged, the enzyme activity of SOD, POD and CAT in the fruits is also obviously reduced, and the effect of resisting gray mold in kiwi fruits is proved. And important gene data support is provided for disease-resistant breeding of kiwi fruits.

The invention relates to the technical field of agricultural biological agents, and particularly provides trichoderma harzianum as well as a compound preparation and application thereof. The trichoderma harzianum has a remarkable inhibition effect on six common plant pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and fusarium oxysporum. The fermentation liquor has a remarkable growth promoting effect on various plants such as pakchoi, spinach and flowering Chinese cabbage when being diluted by 10-100 times, and the fresh weight, the dry weight and the chlorophyll content of the plants can be increased. The compound liquid is formed by compounding trichoderma harzianum fermentation liquor and a hypecoum erectum alkaloid extract with the concentration of 50 ppm, the effective dilution interval of the trichoderma harzianum fermentation liquor in the compound liquid is 10-100 times, and the compound liquid has an inhibition effect on cowpea fusarium wilt germs, promotes growth of cowpeas and nicotiana benthamiana at the same time, achieves integration of disease prevention and growth promotion and has a good application prospect. Wide agricultural application prospects are realized.