150 results about "Botrytis cinerea" patented technology

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.

The invention relates to the technical field of biological prevention and control, and discloses application of a bacillus velezensis P15 fungicide in preservation of harvested strawberries, and the application comprises the step of applying the bacillus velezensis P15 fungicide to the harvested strawberries so as to prevent and control strawberry postharvest diseases caused by pathogenic fungi. The preservation number of the bacillus velezensis P15 is CGMCC (China General Microbiological Culture Collection Center) NO.31995. The bacillus velezensis P15 microbial inoculum is used for preservation of picked strawberries, can efficiently inhibit more than 90% of picked pathogenic bacteria such as botrytis cinerea and the like, has a control effect superior to that of prochloraz, and does not enable the pathogenic bacteria to resist drugs after being used for a long time; the strawberry preservative is harmless to human, animals, plants and the environment, has no microbial agent residue, can be used for fresh keeping of green and organic strawberries, solves the food safety problem of chemical preservative residue, and improves the acceptability of consumers.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The present application relates to the technical field of pesticide chemistry and medicinal chemistry, and particularly relates to a substituted pyrazole-containing ortho-hydroxy aryl formamide derivative, a preparation method and application thereof. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative is prepared from a 5-amino pyrazole intermediate (I) and a different substituted ortho-hydroxy aryl formic acid (II) as raw materials through an amide condensation reaction. The substituted pyrazole-containing ortho-hydroxy aryl formamide derivative prepared by the present application has a broad-spectrum inhibitory activity on plant pathogenic fungi, and particularly has excellent inhibitory activity on Valsa mali, Sclerotinia sclerotiorum and Botrytis cinerea, and is expected to be developed into a new type of green fungicide with high efficiency, safety, economy and environmental friendliness.

The invention discloses a transcription factor AcWRKY84 gene for regulating and controlling gray mold resistance of kiwi fruits. After the expression of the gene is silenced, the gray mold resistance of kiwi fruits can be remarkably reduced. The AcWRKY84 gene is cloned, and the anti-gray mold effect of the AcWRKY84 gene in kiwi fruits is proved, specifically, after an experimental group is inoculated with viruses, compared with a control group, the disease symptoms of the fruits of the experimental group are aggravated, the infected area is obviously enlarged, and the enzyme activities of SOD, POD and CAT in the fruits are obviously reduced compared with the control group; sufficient gene resources can be provided for a kiwi fruit gray mold resistance gene pool, so that the possibility of successfully breeding high-gray mold-resistance varieties subsequently is greatly increased.

This invention discloses the application of the FAD2 gene of *Botrytis cinerea* in regulating the growth, pathogenicity, and stress resistance of *B. cinerea*. The FAD2 gene of this invention is derived from *B. cinerea*. By constructing a knockout gene fragment of the FAD2 gene and introducing it into the protoplasts of *B. cinerea*, a knockout mutant of the FAD2 gene was obtained. Studies have found that the FAD2 gene plays an important role in regulating the growth of *B. cinerea*, influencing its ability to withstand high salinity and osmotic stress, and significantly reducing the pathogenicity of *B. cinerea* mycelium to tomato fruits and leaves. Therefore, FAD2 can serve as a key gene for studying the pathogenic mechanism of the disease and can be used as a target for fungicides. This can be used to develop fungicides to control *B. cinerea* or for gene function research on the pathogen, contributing to the breeding of new plant disease-resistant varieties and showing broad application prospects in plant fungal disease research.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

This invention belongs to the field of agricultural biotechnology, and specifically relates to a strain of *Dictyophora indica* Phd01, its inoculant, and its application. *Dictyophora indica* Phd01 is classified and named... Phallus dongsun The strain was deposited on January 23, 2026, at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2026233, located at Wuhan University, Wuhan, China. The fermentation broth of this strain significantly inhibited the mycelial growth of *Botrytis cinerea* and *Anthracnose spp.*; it showed a relative control efficacy of 42.31% against gray mold in peppers and enhanced the oxygen free radical scavenging capacity of pepper fruits, effectively extending the storage time of pepper fruits; the relative control efficacy against anthracnose in strawberries reached 31.03–40.23%, and it prolonged the time of strawberry disease onset. This indicates that the fermentation broth of *Dendrobium nobile* Phd01 can significantly inhibit strawberry and pepper rot, thereby extending shelf life, and has the potential to be developed into an environmentally friendly biological agent.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The application belongs to the field of agricultural fungicide compound, discloses a composition containing new white folium altheae alkaloid derivative Z-24 and anthraquinone components (emodin, emodin methyl ether, chrysophanol, aloe emodin) in rhubarb and mixed concentration thereof. The application of the composition in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, pyricularia oryzae and phytophthora capsici is disclosed, and the mass percentage of new white folium altheae alkaloid derivative Z-24 and anthraquinone components is 1:500, 1:100, 1:50 or 1:25. The fungicidal composition disclosed in the application has the advantages of wide fungicidal spectrum, low toxicity, effective delay of drug resistance of plant pathogens and the like, and has the value of further research and development.

The invention discloses a quinolinic acid diaryl ether derivative, a preparation method and application thereof and a bacteriostatic agent or bactericide, relates to the technical field of medicinal chemistry, solves the problems that existing pesticides are high in toxicity and not environmentally friendly, easily cause drug resistance of pathogenic bacteria and possibly have negative effects on the environment and an ecological system, can efficiently prevent and treat fungal diseases of plants, and also can effectively prevent and treat the pathogenic bacteria of the plants. The quinolinic acid diarylether derivative has the advantages that the quinolinic acid diarylether derivative has excellent inhibition activity on agricultural fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum and fusarium pseudograminearum, and the quinolinic acid diarylether derivative has the characteristics of simple synthesis process, cheap and easily available raw materials, good control effect, wide bactericidal spectrum and the like.

The invention belongs to the technical field of pesticide chemistry, and relates to a quinoline derivative containing a piperazine structure and a preparation method and application thereof. The invention provides a quinoline derivative containing a piperazine structure, and the quinoline derivative has broad-spectrum inhibitory activity on phytopathogens such as plant pathogenic fungi, especially has excellent inhibitory activity on valsa mali, sclerotinia sclerotiorum, botrytis cinerea, gaeumannomyces graminis and magnaporthe oryzae. The bactericide is expected to be developed into a novel green bactericide which is efficient, safe, economical and environment-friendly.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The application discloses a novel preparation for inhibiting botrytis cinerea and application thereof, and relates to the technical field of bacteriostatic drugs, and has the technical scheme as follows: the active ingredient of the preparation is composed of isoeugenol and bacillus velezensis fermentation liquor.The half bacteriostatic concentration EC50 value of the novel preparation reaches 59.7 ug / mL, and the bacteriostatic capacity is higher than that of the existing technology of bacto in the same concentration.Based on the influence on the membrane permeability of filamentous fungi, the application can synergistically inhibit the pathogenic activity of postharvest fungi of fruits, and is expected to be used for preparing a novel preparation for preventing and treating high-sugar fruits after harvesting.The application is simple to use, environment-friendly, can be directly smeared on the surface of fruits in the planting stage, can not be directly contacted with the fruits in the storage period, and can provide the bacteriostatic capacity for the storage period by using the fumigation mode.The application provides a new choice for the biological prevention of fruits, and has good economic benefits and social benefits.

According to the application of the kiwi fruit transcription factor AcbZIP87 gene in gray mold resistance, after the expression of the gene is inhibited, the resistance of kiwi fruit to gray mold can be remarkably reduced. A gene coding series of the cloned AcbZIP87 gene is shown as SEQ ID NO. 1, and the effect of the AcbZIP87 gene in resisting gray mold in kiwi fruits is proved, specifically, compared with a control group after virus inoculation, the disease symptoms of obtained transgenic fruits are aggravated, the infected area is obviously enlarged, the enzyme activity of SOD, POD and CAT in the fruits is also obviously reduced, and the effect of resisting gray mold in kiwi fruits is proved. And important gene data support is provided for disease-resistant breeding of kiwi fruits.

The application of an endogenous yeast and its application in resisting apple botrytis blight belongs to the field of microorganism technology. In order to solve the technical problems that apple is easily infected by botrytis cinerea to cause botrytis blight during transportation and storage, and the adverse effects of chemical fungicides on environment and human health, the application screens an endogenous yeast from apple, which is identified as Bullera alba through morphological and molecular biological analysis. The experiment proves that the cell suspension of the strain PGY-2 has a control effect on postharvest botrytis blight of apple, and can well colonize at the apple wound, can antagonize the botrytis blight pathogen by forming a biofilm and inducing the activity of various defense enzymes, and the strain PGY-2 can be used for preparing a fungicide for preventing and treating postharvest botrytis blight of apple.

The invention discloses an application of a strawberry transcription factor FaWRKY21 in gray mold resistance after strawberry harvesting, and is characterized in that a nucleotide sequence of a gray mold resistance related gene FaWRRKY21 after strawberry harvesting is as shown in SEQ ID NO.1, and an amino acid sequence is as shown in SEQ ID NO.2. The invention further provides a method for improving gray mold resistance of strawberries after strawberry harvesting, and the method is realized by improving the expression quantity of the FaWRKY21 gene in strawberry cells. Specifically, a recombinant vector containing the strawberry transcription factor FaWRKY21 coding gene is transferred into strawberries through agrobacterium, and transgenic strawberry fruits overexpressed by the strawberry transcription factor FaWRKY21 coding gene are constructed and have the advantages that disease-resistant defense response of the strawberries can be remarkably activated, the morbidity is effectively inhibited, the scab diameter is reduced, and the disease resistance of the strawberries is improved. Therefore, the resistance of strawberries to the postharvest gray mold is improved.

The present application belongs to the technical field of biological control, and particularly relates to a microbial agent for preventing and treating gray mold of crops, and a preparation method and application thereof. The present application provides a microbial agent for preventing and treating gray mold of crops, which comprises a microbial strain and / or a fermentation broth of the microbial strain; the microbial strain comprises one or more of Bacillus subtilis JD-L9, Pseudomonas lundensis DS-PZ-P18 and Pseudomonas chlororaphis HQ-CZ-P12; wherein the Pseudomonas chlororaphis HQ-CZ-P12, the Pseudomonas lundensis DS-PZ-P18 and the Bacillus subtilis JD-L9 have a good inhibition rate on the pathogenic fungus of gray mold, and after being compounded into a compound microbial agent, the compound microbial agent is sprayed on seedlings of medicinal materials, so that the incidence of gray mold during seedling raising is significantly reduced, pesticide residues of the medicinal materials are reduced, and the safety of the medicinal materials is improved.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

A transcription factor for regulating resistance of kiwifruit to botrytis blight AcWRKY84 The application relates to a gene, and after the expression of the gene is silenced, the resistance of kiwifruit to botrytis blight is significantly reduced. AcWRKY84 The application clones the gene and proves the role of the gene in resisting botrytis blight in kiwifruit, and specifically, after the experimental group is inoculated with the virus, the disease symptoms of the experimental group are more serious, the infected area is obviously increased, and the SOD, POD and CAT enzyme activities in the fruit are significantly reduced compared with those of the control group, so that sufficient gene resources can be provided for a botrytis blight resistance gene library, and the possibility of realizing successful breeding of a high botrytis blight resistance variety is greatly increased.

This invention discloses the tea plant miRNA miR408-p5 and its application and method in improving plant disease resistance. By constructing a silencing expression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, exhibiting strong fungal inhibitory activity against *Botrytis cinerea*. Conversely, by constructing an overexpression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, showing significantly weakened resistance to *Botrytis cinerea*. Therefore, silencing miR408-p5 expression can improve plant disease resistance, and introducing the miR408-p5 silencing expression vector into plants such as tobacco, tea, or vegetables to enhance plant disease resistance has broad market application prospects.

A polymeric masterbatch is provided to prepare an antimicrobial material and an antimicrobial air filter prepared with said material, which serves to filter air and reduce the growth of bacteria and fungi such as E. coli, S. aureus, Botrytis cinerea, and Aspergillus niger.

The invention relates to a trichoderma asperellum sourced Cerata-plaatanin (CP) family protein CP3 and application thereof, and belongs to the technical field of biological prevention and control of plant diseases. The CP3 protein is derived from Trichoderma asperellum, belongs to a Cerato-platanin family and has a conservative CP structural domain, the amino acid sequence registration number of the CP3 protein is XP024756165.1, and the registration number of a coding gene of the CP3 protein is 36612810 (Gene ID). The protein is a secreting type protein, has signal peptide and can be positioned in tomato cell nucleuses. The invention proves that the disease spot area of tomatoes infected with Botrytis cinerea can be reduced by 71% after the CP3 protein is exogenously applied; after tomatoes are treated by the trichoderma asperellum CP3 overexpression strain, the area of gray mold scabs is remarkably reduced, and the CP3 gene knockout strain has no disease-resistant effect. The CP3 protein and the trichoderma asperellum strain expressing the CP3 protein can be used for preparing biological agents for enhancing the botrytis cinerea resistance, application modes comprise rhizosphere application, root system inoculation and the like, adverse effects on tomato growth are avoided, and a new effective means is provided for green prevention and control of the botrytis cinerea.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.