1002 results about "Botrytis cinerea" patented technology

The invention relates to a method for controlling plant diseases caused by the fungi Botrytis cinerea and Alternaria alternata, by applying to a growing plant or to fruit or vegetables before or after harvesting, a composition which comprises an effective amount for controlling said fungi of at least one oligosaccharide ingredient, active against Botrytis cinerea and Alternaria alternata, and selected from oligosaccharides obtainable by hydrolysis of chitin, beta -glucan and other similarly active polysaccharides, excluding chitosan, of cell walls of fungi, yeasts, marine plants and exoskeletons of arthropods, The composition also forms part of the invention, as does an analogous method and composition for a method for controlling plant diseases caused by Botrytis cinerea, and utilizing at least one oligosaccharide ingredient, active against Botrytis cinerea selected from oligosaccharides obtainable by hydrolysis of chitosan, and having a molecular weight within the range of about 500 to about 10,000 daltons, provided that in this instance the composition excludes acetic acid.

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

The present invention relates to a composition for preventing at least one plant disease resulted from infection of plant pathogens and a method for preparing the same. Particularly, the composition contains extract of at least one plant selected from the group consisting of Coptis chinensis Franch., Phellodendron genus plant, Sanguinaria canadensis and Chelidonium majus var. asiaticum which are natural plants having anti-plant pathogen activity or berberine which is purified therefrom. The composition of the present invention can be effectively used for preventing disease pathogens such as plant powdery mildew, anthracnose of pepper, damping-off of cucumber and gray mold rot of strawberry resulted from infection of plant pathogens such as Colletrotrichum gloeosporiodes, Pythium ultimum or Botrytis cinerea. For an alternative method for preparing the composition comprising berberine and/or derivatives thereof, the present invention provides various optimal culture condition and two phases cultivation methods using Phellodendron genus plant.

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.

The invention discloses an antibiotic Bacillus amyloliquefaciens with powerful bacteriostasis and wide bactericidal spectrum. The strain has been preserved in the China General Microbiological Culture Collection Center and the taxonomy name of the strain is: Bacillus amyloliquefaciens with preservation number CGMCCNo.4777. The antibiotic Bacillus amyloliquefaciens has powerful bacteriostasis, wide bactericidal spectrum, high inhibition rate up to above 93.5% on the rice blast fungus, the Botrytis cinerea and the Phytophthora parasitica; the Bacillus amyloliquefaciens also has very strong stress resistance, and very strong resistance to the acid and alkali, and is suitable to be used as microbial bactericide.

The invention relates to a high-efficiency Trichoderma harzianum strain for controlling plant fungus diseases and promoting plant growth. The Trichoderma harzianum strain has been collected at the China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7230. The Trichoderma harzianum strain provided by the invention has the advantages of high capacity for inhibiting plant pathogenic fungus growth, no environment pollution and high ecological safety, can be widely used for controlling plant pathogenic fungi, especially Pythium spp., Fusarium spp. and Botrytis cinerea, and has high application value in biological control on melons, fruits, vegetables and crops. The high-efficiency Trichoderma harzianum strain can be used independently, or used together with other organic and inorganic nutrients required by plant growth; and the prepared biological compound fertilizer has obvious effects on promoting growth, inhibiting premature senility, preventing diseases, increasing yield and improving quality.

The invention relates to the technical field of screening of functional microorganisms, and particularly provides a bacillus amyloliquefaciens strain, wherein the collection number is CCTCC NO: M2015075. Growth and propagation of pathogenic bacteria such as alchemilla japonica podosphaera leucotricha, botrytis cinerea, fusarium oxysporum and rhizoctonia solani can be significantly inhibited by bacillus amyloliquefaciens; plant diseases such as strawberry powdery mildew and cucumber gray mold are effectively prevented and treated; and the effect is significant. The bacillus amyloliquefaciens can be applied to prevention and treatment of the plant diseases as an antibiological inoculant, and can also be compounded with other bacilli and moulds with bacteriostatic action to prepare a compound microorganism preparation; the prevention and treatment efficiency on the plant diseases such as powdery mildew, gray mold, brown blotch and fruit rot is 85%-90%; and the application prospect is wide.

The invention relates to a bacillus subtilis preparation for preventing and controlling soil-borne diseases, as well as a preparation method thereof. The bacillus subtilis preparation is prepared by taking the thalli and metabolites of bacillus subtilis B579 with preservation number of CGMCC No.2270 as an effective active constituent which are matched with agriculturally acceptable carrier and a heat-resistant protective agent; the thalli content of the effective active constituent is 8 to 80 billion thalli per gram; and the mass content of the heat-resistant protective agent is 3 to 5 percent. The bacillus subtilis preparation can effectively prevent and control cucumber drooping diseases, cucumber wilt, eggplant root rot, eggplant damping-off, pepper blight, tomato basal stalk rot, grape botrytis cinerea and banana anthracnose. The bacillus subtilis preparation has a control efficiency more than 70 percent, has obvious effect of promoting plant growth, and is characterized in high efficiency, wide antimicrobial spectrum, simple use, low cost, no environmental pollution and improvement on the micro-ecological environment of soil.

The invention discloses a gliocladium spp. strain for suppressing botrytis cinerea pers and relates to the gliocladium spp. strain. The invention provides a new strain of gliocladium spp., which can be used for suppressing the botrytis cinerea pers and have the effect of suppressing a plurality of other fungal diseases, thereby laying a material foundation for biological control of the botrytis cinerea pers and culture of new species for anti-botrytis cinerea pers by utilizing the biological technology. The gliocladium spp. strain for suppressing the botrytis cinerea pers is gliocladium roseumWY-1 which is collected in China General Microbiological Culture Collection Center with the collection No. of CGMCCNo. 1977. The gliocladium roseum WY-1 can significantly suppress pathogens of the botrytis cinerea pers, fulvia fulva, phytophthora parasitica, verticillium dahliae of tomato, fusarium wilt of tomato, valsa sordida nit, poplar fusarium wilt and blackcurrant leaf spot, and suppress the growth of mycelia of the pathogens.

The invention relates to a Bacillus subtilis strain YB-05, and a preparation method of an inoculant of the Bacillus subtilis strain YB-05. The Bacillus subtilis strain YB-05 is preserved at China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNO.6498. The microbial inoculant is a pulvis prepared through the spray dry preparation of a fermentation solution of the Bacillus subtilis strain YB-05. The YB-05 strain and its inoculant have the advantages of wide antibacterial spectrum, good antimicrobial effect, and strong inhibition effects on the wheat take-all disease, tomato Botrytis cinerea, wheat sheath blight fungi, Phytophthora melonis and the like; and the inoculant has the advantages of high efficiency, non-toxicity, simple use, and no environmental pollution.

The invention concerns a method and a device for the objective qualitative analysis of liquid vinification compositions, comprising memory means (8) in which are recorded calibrating values of spectroscopic criteria for a group of characteristic parameters comprising the concentration levels of at least a compound produced by Botrytis cinerea, at least a compound produced by yeasts, at least a compound produced by the acetic bacteria, and at least a compound produced by lactic acid bacteria, means (1, 2, 3, 4, 6) for producing a continuous infrared spectrum, and means (7) for calculating the value (V_P) of each characteristic parameter in the composition from the spectrum.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention discloses a bacillus subtilis B2 microbial agent, a compound microbial agent and an application thereof, wherein the microbial agent is obtained through liquid fermentation of bacillus subtilis B2 in a beef peptone culture medium, and the living bacteria number is 1.007*1010-1.73*1012cfu/ml; the compound microbial agent comprises the bacillus subtilis B2 and at least one strain of bacillus sp B3, bacillus sp B4, bacillus sp B5 and bacillus sp B6; the total living bacteria number is 1.33*109-4.06*1010cfu/ml; and the microbial agent and the compound microbial agent can inhibit pathogenic bacteria such as sclerotium bacterial of eggplants, botrytis cinerea of tomatoes and the like. The microbial agents and the application can overcome the defects of having poor environmental protection and poor safety, being easy to produce drug resistance and the like of the chemical pesticide in the prior art so as to realize the advantages of having good environmental protection and good safety and being difficult to produce the drug resistance.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention discloses Bacillus atrophaeus and an application thereof. The Bacillus atrophaeus JZB120050CGMCC No.10919 has stable, efficient and broad-spectrum antibacterial properties. The Bacillus atrophaeus can generate a biological membrane, has strong capability of generating siderophore, chitinase, cellulase and protease, which shows that the strain has relatively strong biocontrol potential. The control effect of fermentation liquor of the strain to tomatoes inoculated with botrytis cinerea for culturing 11d is 100% (the control effect of a 5-time diluent treatment group of JZB120050 strain fermentation liquor is 48%, the control effect of a treatment group of JZB120028 strain fermentation liquor is 50%, the control effect of a 5-time diluent treatment group of JZB120028 strain fermentation liquor is 26%, and the control effects of a blank control group, a 1000-time liquid treatment group of 3% polyoxin, and 1000-time liquid treatment group of 50% carbendazim are 0%), which shows that the strain has an effective inhibitory action on the botrytis cinerea. The strain is safe to human and domestic animals, does not have environmental pollution problems, is simple in culture condition, easy to store and suitable for industrial production, and has a good development application prospect.

The invention discloses a bacillus velezensis TK2019 microbial agent and application thereof. The bacillus velezensis TK2019 is separated from rhizosphere soil of the cotton field of the 44 regiment in the Tumxuk city of Xinjiang, and the bacillus velezensis TK2019 microbial agent is used for preparing the bacillus velezensis TK2019. When the microbial agent is applied to prevention and treatmentof cotton verticillium wilt, cotton fusarium wilt, botrytis cinerea and red date black spots, the inhibition rate of the strain TK2019 on verticillium dahliae of cotton reaches 94.37 percent, the inhibition rate of the strain TK2019 on four tested pathogenic fungi is 84.83-93.73 percent, and the stock solution and 50-time diluted solution of the bacillus velezensis TK2019 microbial agent have thehighest prevention effects which are 92.86 percent and 91.97 percent respectively. Field tests prove that the prevention effect the TK2019 microbial agent on cotton verticillium wilt is 92.69 percent,which indicates that the Bacillus velezensis TK2019 and the microbial agent thereof provided by the invention have the advantages of high efficiency, wide bactericidal spectrum and the like and havewide values for expanding the application field of microbial strains.

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention discloses a method for preparing bacteria-free pine wood nematodes. The method for preparing the bacteria-free pine wood nematodes comprises the following steps: culturing the pine wood nematodes; preparing pure pine wood nematode eggs; soaking the pine wood nematode eggs in 15% H2O2 for 50-60 minutes; incubating the pine wood nematodes; and culturing the pine wood nematodes and performing bacteria-free identification, thereby preparing the bacteria-free pine wood nematodes. As for the method for preparing the bacteria-free pine wood nematodes, the property of being capable of being adhered on a glass vessel of the pine wood nematode eggs is utilized, a small culture dish is adopted for collecting the eggs, the newly prepared 15% H2O2 is further used for performing sterilization treatment, the bacteria-free pine wood nematode eggs are obtained, the bacteria-free eggs are incubated, mass propagation is further performed on botrytis cinerea, and then the bacteria-free pine wood nematodes are finally obtained. Under sterile conditions, the obtained bacteria-free pine wood nematodes are used for performing an inoculation test on seedlings cultured by spruce bacteria-free tissues, and the result shows that the inoculation of the bacteria-free pine wood nematodes can still enable the bacterial-free seedlings to wilt. The method can be adopted for enabling the pine wood nematodes to be bacteria-free and lose pathogenicity, thereby being the simple and successful method.

The invention discloses Bacillus subtilis strain HMB26553 (Bacillussubtilis), the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCCNo.13211. The present invention also discloses a microbial agent produced by use of the strain and application thereof in the prevention and control of plant diseases such as cotton damping-off and the like. The Bacillus subtilis HMB26553 has high control efficiency on the cotton damping-off, the average control efficiency is above 90%; the microbial agent has good effect perdurability, and has no environmental pollution problems; in addition, the HMB26553 has a wide antibacterial spectrum, and has a good inhibitory effect on cotton rhizoctonia solani kuh, cotton verticillium dahlia, cotton Fusarium oxysporum, tomato botrytis cinerea and cucumber Corynespora cassiicola.

The invention discloses a 2-cyano-3-(substituted) amino-3-phenyl-acrylic acid ester compound, a preparation method and application thereof. The compound has a formula (I) structure and good fungicidal activity, can be used to prevent and treat diseases caused by a plurality of fungi such as fusarium, rust fungi, botrytis cinerea and so on. The compound has the advantages of simple process method, good sterilization effect, and broad market prospect.

The invention discloses Bacillus amyloliquefaciens subsp. plantarum. The classified nomenclature of the Bacillus amyloliquefaciens subsp. plantarum is Bacillus amyloliquefaciens subsp. plantarum B203; the Bacillus amyloliquefaciens subsp. plantarum was collected in China General Microbiological Culture Collection Center on May 25, 2012; and the collection number is CGMCC No. 6156. The Bacillus amyloliquefaciens subsp. plantarum is mainly used for biological control of strawberry anthracnose. The Bacillus amyloliquefaciens subsp. plantarum B203 has a remarkable inhibitory effect on Colletotrichum fragaliae Brooks, strawberry Botrytis cinerea and various pathogenic fungi of poplar canker, and the antagonistic bacterium also has a good control effect on various other plant diseases; the propagation speed is high; the Bacillus amyloliquefaciens subsp. plantarum can be manually cultured, is easy to operate, and can be conveniently produced and applied; the Bacillus amyloliquefaciens subsp. plantarum has the advantages of high stress resistance and the like, and has an important significance for controlling the strawberry anthracnose caused by fungi; and culture conditions are simple, and the Bacillus amyloliquefaciens subsp. plantarum is easy to preserve, can be industrially produced easily, and has a good development and application prospect.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu/g on the solid fermentation medium of wheat bran and corncob nutrient solution.