341 results about "Botrytis cinerea" patented technology

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention relates to the technical field of biological prevention and control, in particular to a trichoderma subatroviride Thp30 strain and application thereof. The trichoderma subatroviride Thp30 is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, and the preservation number is GDMCC No: 66381. The trichoderma pseudoviride Thp30 provided by the invention can inhibit the growth of wheat stem rot pathogen fusarium graminearum, apple anthracnose pathogen colletotrichum gloeosporioides, botrytis cinerea pathogen botrytis cinerea, wax gourd fusarium wilt pathogen fusarium oxysporum and pear anthracnose pathogen colletotrichum gloeosporioides, and promote the growth of wheat, soybean and wax gourd; the wheat basal stem rot can be effectively prevented and treated, and the freezing resistance of wheat can be enhanced.

The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention relates to the field of agricultural products, and particularly discloses a polypeptide for improving plant disease resistance, and the polypeptide is LAND2 small peptide and homologous peptide thereof. The LAND2 small peptide disclosed by the invention is screened and found from an arabidopsis thaliana proteome, and homologous peptides of the LAND2 small peptide are respectively derived from chilies and cucumbers. Tests prove that when the LAND2 small peptide is externally applied to arabidopsis thaliana, the local and systematic resistance of plants to botrytis cinerea can be enhanced, and the LAND2 small peptide has broad-spectrum immunocompetence in chilies and cucumbers. The polypeptide is practically applied to agricultural production, can enhance the disease resistance of plants and improve the yield and quality of the plants, and has practical popularization and application values.

The invention discloses a composition for improving the tolerance of plants to saline-alkali soil and application of the composition. The present invention relates to the field of microorganisms, and provides a composition, which contains a Botrytis cinerea strain 7466 (ACCC 34101), a Chaetomium globosum strain 7126 (ACCC 34102), and a Cladosporium halotolans strain 7309 (ACCC 34103), and a preparation method of the composition, the preparation method of the composition, and the preparation method of the composition, the composition provided by the invention can improve the salt and alkali tolerance of plants. The method is of great significance in saline-alkali soil restoration and improvement and growth promotion of crops such as soybeans.

The invention discloses application of biological silver nanoparticles synthesized based on bacillus velezensis in prevention and treatment of tomato gray mold, and relates to the technical field of prevention and treatment of tomato gray mold. Researches find that the biological silver nanoparticles Bio-AgNPs have a relatively strong inhibition effect on the growth of botrytis cinerea hyphae, and when the concentration of the biological silver nanoparticles Bio-AgNPs is 100 mu g / mL, the growth inhibition rate of the botrytis cinerea hyphae is 100%. The Bio-AgNPs shows a good prevention and treatment effect on the tomato gray mold, after 40 [mu] g / mL of Bio-AgNPs is used for treating tomato fruits and leaves, the scab of the tomato fruits is obviously reduced by 52.21%, and the prevention and treatment effect on the tomato leaf gray mold is 40.19%.

The invention relates to bacillus subtilis Y32, and the preservation number of the bacillus subtilis Y32 is CGMCC (China General Microbiological Culture Collection Center) No.34819. The strain can produce xylanase with high temperature resistance and high stability, various hydrolases such as beta-mannase, protease and the like, exopolysaccharides and botrytis cinerea inhibition, and is resistant to acid, swine bile salt and 10% NaCl. The optimum temperature of xylanase produced by the strain is 55 DEG C, the optimum pH value is 6.0, under the conditions, the half-life period is 12.5 h, the relative enzyme activity is still kept above 80% after incubation for 3 h, and the xylanase has good thermal stability. The strain can also improve the yield of cucumbers and pinellia ternate, and can also improve the laying rate of 70-week-old hyline brown laying hens from 70.4% to 90.1%. The strain has a good application prospect in the fields of agriculture, food processing and cosmetic production.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis NEAU-L05, application of the bacillus velezensis NEAU-L05, antibacterial cyclic lipopeptide Iturin A and application of the antibacterial cyclic lipopeptide Iturin A. The bacillus velezensis NEAU-L05 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, West Beichen Road, Chaoyang District, Beijing, the preservation date is December 19, 2024, and the preservation number is CGMCC No.33130. The bacillus velezensis NEAU-L05 disclosed by the invention can be used for preparing the bacillus velezensis NEAU-L05. The bacteriostasis rate of the bacterial strain to botrytis cinerea of phytopathogen is 67.2%. The bacteriostatic cyclic lipopeptide ItuinA separated from the strain can improve the storage performance of berries, and a basis is provided for development of multiple biocontrol microbial agents with excellent performance.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention relates to talaromyces cordiformis and application thereof. The talaromyces sp. Strain Dz-1118 is found to have an antagonistic inhibition effect on fusarium solani, fusarium oxysporum, fusarium pseudograminearum, fusarium verticillium, fusarium graminearum or botrytis cinerea, so that the talaromyces sp. Strain Dz-1118 can be used for preventing and treating root rot, fusarium wilt, stem rot, spike rot, gibberellic disease or gray mold.

A preparation method of plant compound essential oil comprises the following steps: preparing mixed powder from dried lemon slices, mint and litsea coreana, carrying out carbon dioxide supercritical extraction, and collecting an extract; the preparation method comprises the following steps: dropwise adding a carboxymethyl-beta-cyclodextrin solution into a mixture of eugenol and thymol, carrying out ultrasonic treatment to obtain an ultrasonic mixed solution, standing, centrifuging, collecting supernate, and drying to obtain a white powdery inclusion compound; and adding lecithin, Tween 80, disodium pyrophosphate and deionized water into the extract, heating and stirring, continuing to add the inclusion compound, homogenizing to obtain a nano-emulsion, adding gelatin and propylene glycol, heating and preserving heat, and cooling to obtain the plant composite essential oil. The compound essential oil disclosed by the invention has antibacterial and antioxidant effects, and has relatively strong antibacterial ability on botrytis cinerea and penicillium germs, and the DPPH free radical scavenging rate is greater than or equal to 90%. According to the invention, the active ingredients of the essential oil are wrapped by carboxymethyl-beta-cyclodextrin and synergistically embedded by lipidosome through a nano-embedding technology to realize slow release, so that the antioxidant and antibacterial properties are effectively prolonged.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention relates to an oxadiazole thioformate compound as well as a preparation method and application thereof. The compound has a structure as shown in a general formula (I): # imgabs0 #, an oxadiazole structure is taken as a basis, a thioformate structure with biological activity is introduced into the structure, a series of novel oxadiazole thioformate compounds are synthesized, and the compound provided by the invention has a wide application prospect on various common fungal diseases of crops, such as crop diseases, crop diseases and the like. The bactericidal composition has remarkable control effects on cucumber botrytis cinerea (B.c), blueberry anthracnose (C.a), rice blast (M.o), sclerotium of rape (S.s) and the like. The compound disclosed by the invention shows wide antibacterial activity and has a remarkable inhibition effect on various fungi.

The invention discloses low-temperature-resistant rice endophytic streptomyces with biological prevention and control effects on common crop diseases and application of the low-temperature-resistant rice endophytic streptomyces, relates to the field of microorganisms, and aims to solve the problems that an existing rice blast biocontrol strain cannot play a stable effect under a low-temperature condition and is poor in rice blast control effect. The low-temperature-resistant rice endophytic streptomycete is alfalfa streptomycete F05 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33539. The streptomyces medicaginis F05 disclosed by the invention can stably play a biological prevention and control role at a low temperature of 5-15 DEG C. The compound has broad-spectrum antibacterial performance, is used for inhibiting corn stalk rot bacteria, green bean colletotrichum, rice bakania, botrytis cinerea and rhizoctonia solani, and can also be used for decomposing starch and cellulose.

The invention discloses a C2 spiro indolone compound as well as a preparation method and application thereof, simple and easily available N-indole connected o-alkynyl nitrobenzene compound is used as a raw material, and the C2 spiro indolone compound is obtained by heating reaction in a solvent under the catalysis of a cheap metal copper catalyst. The C2 spiro indolone compound prepared by the invention has a relatively good inhibition effect on phytopathogen, and can be used for preventing and treating botrytis cinerea, sclerotinia rot of colza and botryosphaeria dothidea; the preparation method does not need to use an expensive metal catalyst, does not need an additional reducing agent, and has the advantages of simple operation, high chemical selectivity, wide reaction substrate range and the like.

The present invention discloses a gene for enhancing the resistance of strawberry fruits to Botrytis cinerea and its application, belonging to the technical field of genetic engineering. The nucleotide sequence of the gene of the present invention is as shown in SEQ ID NO.7. The present invention provides a gene for enhancing the resistance of strawberry fruits to Botrytis cinerea. By introducing this gene into strawberry plants and enabling its stable overexpression, the resistance of strawberry fruits to Botrytis cinerea is significantly enhanced. Moreover, this gene has no impact on the quality of strawberry fruits, providing new gene resources and theoretical support for the breeding of strawberry varieties with high resistance to Botrytis cinerea.

The invention discloses a disease-resistant application of an arabidopsis thaliana AtRTP7 gene. The invention relates to application of inhibiting the expression of an arabidopsis thaliana AtRTP7 gene in enhancing the resistance of plants to pathogenic bacteria. The number of the arabidopsis thaliana AtRTP7 gene in an arabidopsis thaliana genome database is AT4G02820. The invention focuses on the S gene AtRTP7 of arabidopsis thaliana, the growth phenotype of the mutant is observed, and inoculation experiment is carried out on the mutant by using sclerotinia sclerotiorum and botrytis cinerea. The result shows that the growth of the AtRTP7 mutant is not obviously influenced, the AtRTP7 mutant shows obvious resistance to sclerotinia sclerotiorum and botrytis cinerea, and the scab area and the pathogenic bacterium biomass of the AtRTP7 mutant are obviously lower than those of a wild plant. Therefore, the S gene AtRTP7 of arabidopsis thaliana can be used as a potential gene resource for green prevention and control of sclerotinia rot of colza.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention relates to the technical field of agricultural biological prevention and control, and discloses a biological prevention and control complex microbial inoculant and application thereof.The biological prevention and control complex microbial inoculant is prepared by the steps that natural bio-based compounds vanillin and vanillic acid serve as main raw materials, and a biodegradable trialdehyde monomer is designed and synthesized; a biodegradable trialdehyde monomer and p-phenylenediamine are used as organic building units to assemble and form an ordered porous crystal structure, and the biodegradable COF material is obtained; the preparation method comprises the following steps: loading a basic complex microbial inoculant consisting of bacillus thuringiensis, bacillus subtilis and streptomyces willerigensis on a biodegradable COF material by adopting an impregnation adsorption method, and coating the surface of the biodegradable COF material with tetraethylenepentamine and tannic acid through copolymerization to form a degradable polytannic acid layer, so as to prepare the slow-release complex microbial inoculant, and the effective active components of the slow-release complex microbial inoculant can be slowly released; the lasting period and the utilization rate of the composite microbial agent can be remarkably improved, and the composite microbial agent shows an obvious inhibition effect on pathogenic bacteria such as fusarium oxysporum and botrytis cinerea and can be applied to prevention and treatment of plant diseases.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention belongs to the technical field of biology, and discloses Aspergillus tubingensis Pa6 and application of the Aspergillus tubingensis Pa6 in prevention and treatment of fruit tree diseases in order to improve the biological prevention and treatment effect of the fruit tree diseases, the strain number of the Aspergillus tubingensis is Pa6, and the registration number of the Aspergillus tubingensis in the General Microbiological Center of China Committee for Culture Collection of Microorganisms is CGMCC No.41994. The invention further discloses an application of the Aspergillus tubingensis Pa6 in the prevention and treatment of the fruit tree diseases. The Aspergillus tubingensis can prevent and / or treat strawberry gray mold, strawberry black spot or pear tree rot, or can be used for preparing products for preventing and / or treating strawberry gray mold, strawberry black spot or pear tree rot. The bacterial strain also has a relatively good antibacterial effect on fungi such as strawberry botrytis cinerea, strawberry black spot pathogen, apple ring spot pathogen, apple colletotrichum gloeosporioides, pear rot pathogen and the like.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlERF098 protein and a coding gene thereof in regulating and controlling steroid alkaloid content to influence botrytis cinerea resistance. According to the application, the resistance of tomatoes to gray mold is enhanced by regulating and controlling the metabolism of steroid glycoalkaloid through the coding gene. The tomato SlERF098 gene is positioned through whole genome association analysis, and an overexpression strain and an RNA interference strain of the tomato SlERF098 gene are constructed. Experiments prove that the SlERF098 can obviously activate the expression of the GAMEs gene in the SGAs metabolic pathway. In addition, overexpression of the SlERF098 gene can improve the content of SGAs metabolites in tomatoes and the resistance to gray mold, while RNA interference strains present an opposite trend. The invention provides a new target for research on disease-resistant molecular breeding and plant immunity-metabolism coordinated regulation and control, and has important agricultural application value.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.