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Microbial bactericide and application thereof

A microbiological and bacteriostatic technology, applied in the direction of microbes, bactericides, biocides, etc., can solve the problems of pesticide residues, harmful pathogenic bacteria resistance, high toxicity of chemical pesticides, and destruction of biodiversity, etc., to achieve strong resistance, bactericidal Broad-spectrum, easy-to-grow effects

Active Publication Date: 2012-07-25
LONGDENG CHEM XIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] China is a large agricultural country, and the prevention and control of diseases, insect pests and weeds is an important link in agricultural production. At present, the control of crop diseases still mainly depends on chemical fungicides. Pollution is becoming more and more serious. At the same time, chemical pesticides are often highly toxic, killing natural enemies and destroying biodiversity. This provides conditions for more serious pests and diseases to occur rampantly, resulting in a vicious circle in pest control.
Pesticide residues caused by continuous drug use are harmful to the human body and the drug resistance of pathogenic bacteria is causing great concern

Method used

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  • Microbial bactericide and application thereof
  • Microbial bactericide and application thereof
  • Microbial bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Single spore isolation of antagonistic strains

[0020] Select a strain with a broad bactericidal spectrum and good antagonistic properties for single spore isolation. The specific steps are as follows:

[0021] (1) Make a culture medium plate;

[0022] (2) Add the strain (without culture medium) to 10ml of sterile water to make a 1:10 bacterial suspension, shake fully for 10 minutes, then take out 1ml and add 9ml of sterile water to make 10 -2 The bacteria suspension, and so on, made 10 -3 、10 -4 、10 -5 、10 -6 bacterial suspension;

[0023] (3) Use a pipette gun to take 0.5ml of the diluted bacterial suspension and add it to the medium plate, spread it evenly with a spatula, and set 3 replicates for each treatment. Incubate at 30°C for 7 hours;

[0024] (4) Select a plate with 5-20 colonies, number the colonies, pick the single-spore colonies into the LB slope and culture them for 3 days, then put them into a 4°C freezer and store them.

[0025] The a...

Embodiment 2

[0027] Example 2 Antagonistic bacteria Bacillus amyloliquefaciens EC 50 Determination of

[0028] EC 50 That is, the half effective concentration refers to the concentration that causes 50% of the test organisms to produce a specific reaction, or a certain reaction index is inhibited by half.

[0029] Taking rice blast as the test object

[0030] 1) Cultivate rice blast target bacteria and prepare bacteria cake;

[0031] 2) Liquid fermentation of Bacillus amyloliquefaciens, filter the supernatant, dilute a series of concentrations 10ul / ml, 20ul / ml, 40ul / ml, 80ul / ml, 160ul / ml, take 1.5ml for each concentration, add to 13.5 ml PDA is used to prepare the poisonous medium, and the concentration of the fermentation broth is 1ul / ml, 2ul / ml, 4ul / ml, 8ul / ml, 16ul / ml;

[0032] 3) Transplant the bacteria cake into the poisonous medium, and take 1.5ml of sterile water as a control, repeat each treatment 3 times, and culture at 28°C for 3 days;

[0033] Measure the colony diameter by...

Embodiment 3

[0038] Example 3 Bacillus amyloliquefaciens inhibits several pathogenic bacteria

[0039] First scrape the spores of pathogenic bacteria to make a spore suspension, and the spore suspension (1×10 6 cells / mL) mixed with PDA medium that had been melted and cooled to about 45°C to make a plate. Pick Bacillus amyloliquefaciens activated at 30°C for 24 hours and spot-splice it on the pathogen plate, culture it at 28°C for 72 hours, and observe the inhibition zone. It is also possible to arrange the Bacillus amyloliquefaciens and the pathogenic bacteria into a criss-cross arrangement (the pathogenic bacteria are in the center of the cross, and the Bacillus amyloliquefaciens are at the two ends of the cross). Cultivate for 3 to 4 days, and observe the size of the inhibition zone.

[0040] The inhibitory effect of Bacillus amyloliquefaciens on the mycelium growth of 10 kinds of fungi including rice blast, wheat head blight, tomato gray mold, and apple rot is shown in Table 2.

[00...

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Abstract

The invention discloses an antibiotic Bacillus amyloliquefaciens with powerful bacteriostasis and wide bactericidal spectrum. The strain has been preserved in the China General Microbiological Culture Collection Center and the taxonomy name of the strain is: Bacillus amyloliquefaciens with preservation number CGMCCNo.4777. The antibiotic Bacillus amyloliquefaciens has powerful bacteriostasis, wide bactericidal spectrum, high inhibition rate up to above 93.5% on the rice blast fungus, the Botrytis cinerea and the Phytophthora parasitica; the Bacillus amyloliquefaciens also has very strong stress resistance, and very strong resistance to the acid and alkali, and is suitable to be used as microbial bactericide.

Description

technical field [0001] The invention relates to a microbial bactericide and its application, belonging to the technical field of microbial pesticides. Background technique [0002] Biological pesticides refer to preparations that use living organisms or their metabolites to kill or inhibit agricultural pests. Microbial pesticides refer to substances that are processed by microorganisms and their metabolites and have pesticide activities such as insecticidal, bactericidal, weeding, rodent killing or regulating plant growth. Microbial pesticides have good effects in controlling diseases and insect pests, are non-toxic to humans and animals, do not pollute the environment, and have no residues. They can maintain the excellent quality of agricultural products, are safe for natural enemies of pests and beneficial organisms, and help maintain ecological balance. The raw materials are mainly agricultural and sideline products with a wide range of sources. The production process is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 王新安何梅侠周小军董彪
Owner LONGDENG CHEM XIAN
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