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Metschnikowia pulcherrima inhibiting fungi and application thereof

A technology of yeast and mechi, applied to mechi yeast and its application field, can solve the problems of peach fruit damage, economic loss of fruit farmers, shedding, etc.

Inactive Publication Date: 2014-03-19
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2000, peach brown rot has increased in a large area in Beijing, and it has become more serious year by year. Among them, in the peach orchard where the disease was severe in 2005, peach fruits were damaged and a large number of peaches fell off. There were no obvious symptoms when picking. More than 50% of the fruits were picked 2-3 days after picking. of the fruits are rotted due to brown rot infection, which causes serious economic losses to the fruit growers
[0007] Metschnikowia pulcherrima is widely distributed in nature, but there is no report on Metschnikowia pulcherrima for controlling peach brown rot and apple gray mold in China

Method used

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  • Metschnikowia pulcherrima inhibiting fungi and application thereof
  • Metschnikowia pulcherrima inhibiting fungi and application thereof
  • Metschnikowia pulcherrima inhibiting fungi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. Isolation and strain identification of Metschnikowia pulcherrima DPP2 of the present invention

[0049] 1. Sample collection

[0050] Apples are collected from organic orchards in northern China.

[0051] 2. Isolation and screening of strains and antagonistic screening

[0052] Isolate strains from organic fruits, use conventional gradient dilution coating to isolate, use two kinds of media, PDA (containing streptomycin sulfate) and YPD agar (containing streptomycin sulfate), to culture at 28°C respectively, and pick colonies Bacteria with large morphological differences and can be purely cultured were purified and preserved on YPD agar medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with Peach brown rot as the target pathogen, and finally a strain with strong antibacterial activity was obtained. Yeast strain, named DPP2.

[0053] 3. Identification of strains

[0054] Morphological, physiological and b...

Embodiment 2

[0056] Example 2. Determination of the Bacteriostatic Spectrum of Metschnikowia pulcherrima (Metschnikowia pulcherrima) DPP2 of the present invention

[0057] Pathogens tested:

[0058] Peach brown rot fungus - Monilinia fructicola (wint.) Rehm;

[0059] Botrytis cinerea Per.ex Fr, the postharvest botrytis cinerea of ​​apples;

[0060] Penicillium expansum Link, 1809, Postharvest Penicillium of Apple

[0061] Penicillium expansum Link, 1809, Postharvest Penicillium of Pear

[0062] Peach postharvest brown rot, apple postharvest cinerea and apple postharvest penicillium were selected as indicator fungi, and the plate confrontation culture method was adopted.

[0063] The specific operation is as follows: Metschnikowia pulcherrima DPP2 strain was cultured on PDA medium at a constant temperature of 28°C for 48h. Phytopathogenic fungi were cultured on a PDA plate at a constant temperature of 25°C for 3-4 days, and a 0.7cm-diameter sterile stainless steel punch was punched from...

Embodiment 3

[0066] Inhibition of Metschnikowia pulcherrima DPP2 on rot lesions caused by four tested plant pathogenic fungi.

[0067] After the peaches were picked, they were treated with DPP2 bacterial solution (the concentration of the bacterial solution was 10 7 cfu / ml, 10 8 cfu / ml, 10 9 cfu / ml) soaked for 30-60 seconds, then air-dried at room temperature, and then stored in the dark at a temperature of 1°C and a relative humidity of 95%. After storage for 21 days, compared with the control group, when the bacterial solution concentration was 10 7 cfu / ml, its control effect is 45%, when the concentration of bacteria solution reaches 10 8 When cfu / ml, its control effect is 65%, when the concentration of bacteria solution reaches 10 9 When cfu / ml, its control effect reaches 68%.

[0068] After the apples were picked, the DPP2 bacterial solution (the concentration of the bacterial solution was 10 7 cfu / ml, 10 8 cfu / ml, 10 9 cfu / ml) soaked for 30-60 seconds, then air-dried at room ...

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Abstract

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

Description

technical field [0001] The invention relates to a fungus-inhibiting Metschnijs and its application, which belongs to the technical field of microbial application. Background technique [0002] Plant fungal diseases have brought huge losses to agricultural production, and fruit postharvest fungal diseases are an important type of disease, resulting in a large reduction in fruit production, seriously affecting the edible value and market value of fruits, and resulting in the economic income of fruit farmers. reduce. In developed countries, the losses caused by post-harvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by post-harvest diseases account for more than 30% of the total. . The occurrence of postharvest fruit diseases has seriously affected modern agricultural production, especially the production and processing of high-end fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04A01P3/00C12R1/645
Inventor 张殿朋刘伟成卢彩鸽刘霆董丹张涛涛裘季燕吴慧玲卢向阳
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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