35 results about "Penicillium species" patented technology

The invention provides a method for preparing gold-flower Puer tea by inoculating fungus microorganisms. The method is characterized in that: by the means of inoculating mixed fungi which are mainly eurotium cristatum and adding a fermentation promoter, the blooming of the Puer tea is realized, and the gold-flower Puer tea with gold flowers is prepared. In the production process of the Puer tea, besides of the non-dominant bacteria of the eurotium cristatum, the dominant bacteria including the Puer tea such as aspergillus niger, penicillium notatum, rhizopus and microzyme are also inoculated; and in addition, the fermentation promoter is also added, so that not only the bacteria can be quickly bred, but also the operation and control are convenient; and the tea leaves are fermented and uniformly aged and stable in quality. The gold-flower Puer tea prepared by the method has the advantages that: the beneficial effects of the Puer tea and the eurotium cristatum are perfectly combined; the tea is red, thick and bright and has pure aroma and a mellow taste; other health-care effects are further improved; and the quality is improved in essence compared with traditional Puer tea.

An artificial culture method of new balm cricket Penicillium notatum and extraction and application of delivery pains compounds are disclosed. This invention involves declutching and breeding quality new balm cricket Penicillium notatum APC-20 from nature, researching production of the strain, further, defining method of extracting delivery pains compounds to be medicine. The compound is N6-(2- hydroxyethyl) adenosine. Molecular weight is 311.297. Molecular formula is C12H17N5O5. Delivery pains ratio is 99%by the method of mouse test. Principle of delivery pains is particular and cannot induce addiction.

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

The invention belongs to the preparation of a new gene, and in particular discloses a glucose oxidase gene GOD, protein coded by the GOD and pichia pastoris transformed by the GOD. The glucose oxidase gene GOD is prepared by taking 5'-ATGAAGTCCACTATTATCACCTCCA and 3'-CTAGGCACTTTTGGCATAGTCTTCA as specific primers and penicillium notatum as a template through polymerase chain reaction (PCR) amplification. According to the invention, the problem of an existing recombinant glucose oxidase gene in heterologous expression is solved; the total-length GOD gene and a shuttle expression vector constructed by the gene are obtained and the gene and the shuttle expression vector thereof are further transformed to the pichia pastoris, and upon screening and identification, a strain, which is higher in secretion expression of the glucose oxidase than an original strain, is obtained. The invention can greatly save cost and expense for further industrialized production and can improve the economic benefits of the glucose oxidase.

The invention belongs to preparation and applications of enzyme preparations, and particularly relates to a preparation method and applications of glucose oxidase preparations. Penicillium notatum ACCC30443 is inoculated to a fermentation tank of a carbon source, nitrogen source, inorganic salts and trace elements containing fermentation medium, through aerated fermentation, a fermented liquid is prepared, and then, the fermented liquid is subjected to solid-liquid separation, so that a glucose oxidase preparation is prepared. According to the invention, a problem that new pollutions and chemical residues easily exist in the prior art is solved; and the preparation method and applications of a glucose oxidase preparation disclosed by the invention have the advantages that a preparation process is simple, strains and raw materials are safe, and prepared glucose oxidase preparations are easy to use, rapid in aflatoxin B1 degradation speed, and high in degradation rate, and the like.

The invention discloses a method for preparing dioscin by using Penicillum dioscin051016, CCTCC No: M206001, and relates to the preparation of dioscin. The production method comprises: crushing yam, sterilizing, mixing dioscin penicillium, fermenting at pH 4-7 at 25-28 DEG C for 24-36 hours, refluxing extraction with petroleum ether, crystallizing, drying to obtain diosgenin. Compared with the prior art, the present invention has the following advantages: 1. No acidic organic waste water pollutants are produced; 2. The present invention ferments at normal temperature, which reduces production energy consumption and production cost.

The invention discloses a penicillium notatum LJ-2 strain. The preservation date is July 14th, 2008, and the preservation registration number is CGMCC No.2590. The invention also discloses application of the penicillium notatum LJ-2 in preparing flavonoid glycosides by biotransformation mulberry leaf flavonoid glycoside.

The invention discloses a non-oxidized type compound bactericide for water treatment and a preparation method thereof. The non-oxidized type compound bactericide is prepared by the steps of weighing glutaraldehyde, acrylic acid-2-acrylamide-2-methyl propane sulfonic acid copolymer, tetradecyl benzyl dimethyl ammonium chloride, etidronic acid, water, PBTCA, a polymerization inhibitor, sulfonic acid, polyol phosphonate ester, PESA, a dispersant, sodium tungstate, bitertanol, sodium benzoate and acrylic acid-hydroxy-propyl acrylate copolymer in parts by weight, and mixing uniformly. The product is 100% water-soluble, and has a sterilizing rate of 99.9-100% to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella; and the sterilizing rate is 98-100% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 97.9-99.9% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 97.5-99.5% after the bactericide is placed for 1-2 years at room temperature.

The invention relates to a method for preparing antibody used to check lean meat, wherein it comprises: collecting the animal blood and separating serum to prepare full-serum protein solution; the mixing the solution into carbonate baffle liquid; then adding azo liquid, to prepare carrier couple material; adding the carrier couple material and Freund's complete adjuvant into penicillium notatum bottle, vibrating it on mixer to be white color, to form CL Freund's complete adjuvant emulsion liquid; using emulsifying another carrier couple material and Freund's incomplete adjuvant; and planting the prepared CL Freund's complete adjuvant emulsion liquid on animal to be used in immunization; then separating serum to obtain antibody. The invention uses itself protein, especially the full-serum as the immunization carrier of CL, to make the immunity animal generate special antibody whose ELISA effect can reach more than 40thousand; and the immunity gold testing method built from said antibody can quickly test CL of urine sample, while its sensitivity is 40ng / ml.

The invention discloses novel antimicrobial lipopeptide [delta Cl] Plipastatin and a producing strain and application thereof. The antimicrobial substance [delta Cl] Plipastatin is produced by utilizing bacillus subtilis PB2-LCl, wherein the antimicrobial substance is from B.subtilis PB2-LCl and is a generic term of a series of linear antimicrobial lipopeptide compounds which comprise seven components, and it is identified through LC-FTICR-MS that the molecular weights of the seven components are 980 Da, 994 Da, 1,008 Da, 1,022 Da, 1,036 Da, 1,050 Da and 1,064 Da. The antimicrobial lipopeptide compounds have inhibiting effects on escherichia coli, micrococcus luteus, penicillium notatum and peach bacteria erwinia and can prevent and control various plant diseases.

The present invention is process of preparing dioscin with Penicillium dioscin 051016 in the preservation number of CCTCC No. M206001. The preparing process includes the following steps: crushing Chinese yam, sterilizing, mixing with Penicillium dioscin, fermenting at pH4-7 and 25-28 deg.c for 24-36 hr, reflux extracting with petroleum ether, crystallizing and drying to obtain dioscin. Compared with available technology, the present invention has the advantages of no production of acid organic effluent pollutant and normal temperature fermentation in low power consumption and low production cost.

The invention discloses a strain resisting drought, resisting ultraviolet and storage cicada pecilocin (Paecilomyces cicadidae Miquel samson GZDXIFR-M3L) and appliance, which is characterized by the following: growing in culture medium of carbowax -6000; setting the highest tolerance density at 45%; using 30 watt ultraviolet lamp to irradiate at 40 cm site; irradiating at 5 min; making the survival rate bigger than 80%; possessing stronger resistance for imperfect environment. This invention can prevent and cure aphid and powder lice with stab and suck type mouth parts.

The invention discloses a lauryl guanidine acetate bactericide for water treatment and a preparation method thereof. The lauryl guanidine acetate bactericide is prepared by the steps of weighing O-methyl-isourea sulfate, sodium hydroxide, sodium acetate, methanol, lauryl amine, ethanol, a polymerization inhibitor, acetic acid, anhydrous ethanol, dimethyl sulfate, a dispersant, urea, glacial acetic acid, amino trimethylene phosphonic acid and sodium hexametahposphate in parts by weight, and mixing uniformly. The purity of the bactericide is 96-100%; the sterilizing rate to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella is 98-100%; and the sterilizing rate is 97-99% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 96-98% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 94-98% after the bactericide is placed for 1-2 years at room temperature.

The invention discloses a primer sequence which is used to detect the resistance frequency of Penicillium digitatum to imazalil, which comprises three groups of primer. A sense primer of the first group is equipped with a base sequence of SEQ ID No.1 in the sequence table, and an antisense primer of the first group is equipped with a base sequence of SEQ ID No.2 in the sequence table; a sense primer of the second group is equipped with a base sequence of SEQ ID No.3 in the sequence table, and an antisense primer of the second group is equipped with a base sequence of SEQ ID No.4 in the sequence table; a sense primer of the third group is equipped with a base sequence of SEQ ID No.5 in the sequence table, and an antisense primer of the third group is equipped with a base sequence of SEQ ID No.4 in the sequence table. The primer sequence of the invention has very high specificity and lays the foundation of the entire detection system.

The invention discloses a method of enabling exogenous DNA to penetrate cell barriers to enter penicillium notatum resting spores. The method includes three steps: penicillium notatum culturing and spore collection, penicillium notatum spore pretreatment and using of a HDEN method to electrically shock penicillium notatum spores to obtain the penicillium notatum spores with to-be transformed plasmids guided in. Ungerminated spores are used as a starting material for guiding in exogenous molecules, and HDEN electric transformation technology is applied to guide exogenous DNA into the penicillium notatum resting spores, so that a complex step of spore germination can be omitted, steps of preparing protoplast or agrobacterium-mediated transformation in conventional methods are omitted, transformation rate is high, and each transformation reaction system at least can realize effect of not less than 6000 positive transformants.

The invention provides a preparation for degrading printing and dyeing pollution in a natural fabric. The preparation is prepared from, by weight, 10-20 parts of a citrus extract, 5-15 parts of a bacterium degradation preparation, 3-10 parts of maltose, 2-5 parts of betaine, 1-3 parts of baking soda, 2-5 parts of glucose oxidase, 1-3 parts of pectinase, 1-2 parts of cellulase, 2-5 parts of a metalchelating agent, 1-2 parts of tween-80, 1-2 parts of span-80 and 100-120 parts of deionized water. The glucose oxidase is separated from penicillium notatum, the pectinase is separated from aspergillus niger, and the cellulase is separated from basophilic bacteria. The preparation method of the preparation is simple, the raw material sources are wide, the cost is low, and the preparation has a good degradation effect on the printing and dyeing pollution of the natural fabric, is free of pollution, toxicity and harm, has fragrance and is popular among mass consumers.

The invention relates to a group of Notatone A compounds, method for preparation and use thereof, which is prepared from Penicillium notatum B-62 fungus isolated from Qinghai lake mud samples and has novel structure, experiment has proved that the compounds can be used as cell propagation depressant and antineoplastic agent.

The invention discloses a method for producing feeding glucose oxidase by using rice processing by-products as raw materials, which comprises the following steps of: (1) inoculation of penicillium notatum of glucose oxidase with high yield into a microorganism culture dish of 10cm filled with a PDA (Potato Dextrose Agar) culture medium for carrying out cultivation; (2) seed cultivation: sterilizing a liquid seed culture medium at a temperature of 115 to 121 DEG C for 20 to 30 min, and then transferring the activated penicillium notatum in the culture dish in the step (1) to the liquid seed culture medium; (3) preparation of a solid fermentation medium: mixing broken rice and rice bran according to the weight ratio of 10: (1-3), and cooking for 25 to 40 min at a temperature of 90 to 100 DEG C; (4) inoculating cultivation: after spreading the solid fermentation medium for cooling, inoculating penicillium notatum seeds for carrying out cultivation; and (5) after ending the solid fermentation, drying the culture medium at a temperature of 100 to 120 DEG C for 2-4h, and crushing to 40 to 80 meshes. The method disclosed by the invention has the advantages that sources of raw materials are wide, no three wastes (waste gas, waste water and industrial residual) are generated, the process is simple and the vigor of the glucose oxidase reaches up to 200 U.

The invention belongs to the technical field of food production, and discloses a preparation method of a biological enzyme preparation for food production, which comprises the following steps: step 1, strain selection: selecting penicillium notatum as a culture strain, carrying out activity detection on penicillium notatum vegetative mass before culture, and selecting the penicillium notatum vegetative mass with high activity; step 2, fermentation liquor preparation: A, selecting a sterile environment or disinfecting the environment; and B, performing culture medium culture on the penicillium notatum vegetative mass in the environment, wherein the environment temperature is kept at 25-27 DEG C during culture. According to the invention, strains are strictly controlled, excellent penicillium notatum vegetative mass are selected for culture, quality is controlled from the source, a sterile environment is selected during culture, an optimal environment is selected, and a culture solution is added in the culture process, so that sufficient nutritional ingredients can be provided during growth and reproduction of the penicillium notatum vegetative mass, and the activity of the fermentation liquor can be improved by the introduced oxygen, so that the quality of the fermentation liquor is improved.

The invention discloses Bacillus amyloliquefaciens KB with the collection number of CGMCC No. 8179. The Bacillus amyloliquefaciens KB has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, tomato postharvest botrytis cinerea and apple postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, tomato postharvest gray mold and apple postharvest blue mold. The inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are 22mm and 20mm respectively, and the inhibition rate against peach brown rot spots and apple gray mold spots achieves 100%.

The invention relates to a method for treating skin diseases using live “Penicillium notatum” fungus. There is still little known about the “Penicillium notatum” fungus. Live “Penicillium notatum” fungus has the following functions in the treatment process: 1) as a biological producer to develop the most effective antibiotic against (particular) pathological microorganisms acting as the causative agents of a particular disease; 2) as a biological therapeutic instrument for the rational delivery of an antibiotic individually to each pathogenic microorganism; 3) as a biological instrument for the removal of dead and infected human body tissues; 4) as a biological immunizing agent protecting young regenerating skin; 5) as a biological immunizing agent protecting young skin tissue that has not succeeded to establish immunity after regeneration against a repeated attack from the disease. The treatment period for certain infectious skin diseases using live “Penicillium notatum” fungus lasts from three to five days. In the future, it may be possible to use live “Penicillium notatum” fungus for augmenting the immunity lost by people under various specific circumstances: specific conditions of work and day-to-day activity, long-time presence in space, loss of or removal from constant gravitational weight, impaired individual immunity caused by space-related factors, and the emergence of new diseases. It can supplement or replace the immunizing microflora of human internal organs. It is likely that the fungus will be domesticated and new species thereof will be bred artificially and specifically for each organ of the human body. It is essential that mankind learns how to use the live “Penicillium notatum” fungus in the field of medicine in view of the fact that its resilience exceeds the resilience of all life forms known to mankind, including the resilience of the microorganisms of the immunizing human microflora.

The present invention discloses the conversion of non-edible grade organic maize or wheat into monosaccharides by enzyme hydrolysis. The generated glucose at 14-16% is used to produce natural, organic gluconic acid by microbial fermentation of three strains Aspergillus niger NCIM 545, Penicillium notatum NCIM 745 and Penicillium chrysogenum NCIM 709. These strains are improved by unique media constituents and parameters for product yield enhancement along with the reduced time of gluconic acid production by 15-20 h. Further, gluconic acid is fortified with calcium or sodium or magnesium or ferrous to produce respective gluconate salts which were processed by a set of downstream processes including spray drying to obtain in powder form. These organic gluconates have immense applications in food, pharma, feed, and construction sectors for supplying organic source as well as minerals. This route of gluconic acid and its salts production is robust simple, cost-effective and less time taking by using eco-friendly biotechnological processes.

The invention discloses a kit for semi-quantitative allergen screening. The kit comprises a nitrocellulose membrane strip coated with 19 allergens, a biotinylated anti-human IgE antibody, streptavidin labeled by alkaline phosphatase and a substrate solution, the allergens include willow / poplar / elm, common ragweed, mugwort, dermatophagoides pteronyssinus / dermatophagoides farinae, house dust, cat hair, dog epithelium, cockroaches, penicillium notatum / cladosporium / aspergillus fumigatus / alternaria, scandent hop, egg white, milk, peanuts, soybeans, beef, mutton, cod / lobster / scallop, shrimps and crabs. 19 common allergens can be qualitatively and quantitatively detected through one-time detection, and the method has the advantages that the operation is simple, the detection efficiency is greatly improved, the production and use cost is reduced, and the popularization and application of allergen detection are facilitated.