Preparation method and utilization of glucose oxidase preparation
A glucose oxidase and preparation technology, applied in biochemical equipment and methods, oxidoreductase, and microbial-based methods, etc., can solve the problems of no discovery, low ability to remove toxins, and uncertified feeding license, etc., and achieve degradation speed Fast, easy to use, and stable performance
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Embodiment 1
[0031] The preparation method of the glucose oxidase preparation comprises inoculating Penicillium notatum ACCC30443 in a fermenter containing a fermentation medium containing carbon source, nitrogen source, inorganic salts and trace elements to carry out aerated fermentation to make a fermented liquid, and the fermented liquid is subjected to solid-liquid separation to obtain The liquid is glucose oxidase preparation, which is stored at 2-10°C for later use. Fermentation medium is made up of the component of following mass percentage:
[0032] Sucrose 8%, sodium nitrate 0.7%, dipotassium hydrogen phosphate 0.03%, magnesium sulfate 0.06%, potassium chloride 0.06%, the balance is water, natural pH value;
[0033] The technological conditions of aerated fermentation are as follows:
[0034] The inoculum volume is seed volume: fermentation medium volume = 0.9:100 temperature 29°C, ventilation volume 1:0.9vvm, pressure 0.05Mpa, culture period not less than 60 hours, and 721 spect...
Embodiment 2
[0047] The difference between this embodiment and embodiment 1 is:
[0048] Fermentation medium is made up of the component of following mass percentage:
[0049] Sucrose 6%, sodium nitrate 0.5%, dipotassium hydrogen phosphate 0.01%, magnesium sulfate 0.04%, potassium chloride 0.04%, the balance is water, natural pH value;
[0050] The technological conditions of aerated fermentation are as follows:
[0051] The inoculum size is seed volume: fermentation medium volume = 0.7:100, temperature is 27°C, ventilation is 1:0.7vvm, pressure is 0.03Mpa, and the culture period is not less than 60 hours.
[0052] The culture medium used in the preparation of the slant strains is Chasporian culture medium, the temperature is 26° C., and the culture period is 5 days.
[0053] The preparation of described secondary liquid seed comprises the following process steps:
[0054] According to the volume of the primary seed: the volume ratio of the secondary seed medium=1.3:100, the primary see...
Embodiment 3
[0058] The difference between this embodiment and embodiment 1 is:
[0059] Fermentation medium is made up of the component of following mass percentage:
[0060] Sucrose 7%, sodium nitrate 0.6%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.05%, potassium chloride 0.05%, the balance is water, natural pH value;
[0061] The technological conditions of aerated fermentation are as follows:
[0062] The inoculum size is seed volume: fermentation medium volume = 0.8:100, temperature is 28°C, ventilation is 1:0.8vvm, pressure is 0.04Mpa, and the culture period is not less than 60 hours.
[0063] The culture medium used in the preparation of the slant strains is Chasporian culture medium, the temperature is 28° C., and the culture period is 4.5 days.
[0064] The preparation of described secondary liquid seed comprises the following process steps:
[0065] According to the volume of the primary seed: the volume ratio of the secondary seed medium=1.5:100, the primary s...
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