Process of preparing dioscin with dioscin penicillium notatum
A technology of penicillium diosgenin and diosgenin, which is applied in the field of diosgenin preparation, can solve the problems of large amount, environmental and river pollution, and high cost of sewage treatment, and achieve the effect of reducing production energy consumption and production cost
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[0027] The method for preparing dioscin from Penicillum dioscin 051016 (Penicillum dioscin 051016) CCTCC No: M206001 is carried out according to the following steps.
[0028] 1. Clean the yam raw material;
[0029] 2. Crushing the raw material to less than 5mm;
[0030] 3. Sterilize directly at 100°C for 0.5-1 hour, or add 0.5% ammonia water by weight of raw materials, mix well, and place at 25°C-28°C for 1 hour;
[0031] 4. Add Penicillumdioscin 051016, CCTCC No: M206001, with 0.1% of raw material weight at 25°C-28°C, stir evenly, and ferment at 25°C-28°C for 24-36 hours under the condition of pH 4-7;
[0032] 5. Add water that is 4 times the weight of the fermentation material;
[0033] 6. Stir and filter, dry the filter residue at 60°C to 70°C, then add petroleum ether with a boiling range of 60°C to 90°C that is 3 to 5 times the weight of the filter residue;
[0034] 7. Reflux extraction at 60°C for 2 to 3 times, and combine the extracts;
[0035] 8. Recover petroleum ...
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