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A strain of Bacillus amyloliquefaciens that inhibits fungi and its application

A technology of Bacillus amyloliquefaciens and Bacillus, which is applied in the direction of application, chemicals and bacteria for biological control, and can solve the problems of Bacillus amyloliquefaciens, aggravation, shedding, etc.

Inactive Publication Date: 2016-03-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2000, peach brown rot has increased in a large area in Beijing, and it has become more serious year by year. Among them, in the peach orchard with severe disease in 2005, peach fruits were damaged and a large number of peaches fell off. Fruits with no symptoms were seen during picking. After 2-3 days after picking, 50% The above fruits are rotted due to brown rot infection, which causes serious economic losses to fruit farmers
[0006] Bacillus amyloliquefaciens is widely distributed in nature, and there is no report of Bacillus amyloliquefaciens controlling peach brown rot.

Method used

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  • A strain of Bacillus amyloliquefaciens that inhibits fungi and its application
  • A strain of Bacillus amyloliquefaciens that inhibits fungi and its application
  • A strain of Bacillus amyloliquefaciens that inhibits fungi and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Isolation and strain identification of Bacillus amyloliquefaciens KB of the present invention

[0047] 1. Sample collection

[0048] Collect peaches and kiwis from organic orchards in northern China.

[0049] 2. Isolation and screening of strains and antagonistic screening

[0050] Strains were isolated from organic fruits, and separated by conventional gradient dilution coating. Four kinds of media, LB, NA, KB, and CM0002, were cultured at 28°C respectively, and bacteria with large differences in colony morphology and pure culture were selected. Purified and preserved on LB medium, and performed antagonistic primary screening and multiple re-screening with Peach brown rot as the target pathogen, and finally a bacterial strain with strong antibacterial activity was obtained, which was named KB.

[0051] 3. Identification of strains

[0052] The morphological, physiological and biochemical characteristics and some conservative sequences of the strain KB were analyzed. Refer to th...

Embodiment 2

[0055] Determination of the antibacterial spectrum of Bacillus amyloliquefaciens KB according to the present invention

[0056] Test pathogens:

[0057] Peach brown rot fungus-Moniliniafructicola (wint.) Rehm);

[0058] Botrytis cinerea-Botrytis cinerea Per.exFr;

[0059] Botrytis cinerea (Botrytiscinerea Per.exFr);

[0060] Postharvest apple penicillium pathogen-Penicillium expansum Link (Penicillium expansum Link, 1809)

[0061] Postharvest peach brown rot pathogen, postharvest apple gray mold, and apple postharvest penicillium were selected as indicator fungi, and the plate confrontation culture method was adopted.

[0062] The specific operation is as follows: Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) KB strain was cultured on LB medium at 28°C for 48h. Plant pathogenic fungi were cultured on a PDA plate at a constant temperature of 25°C for 3-4 days. From the edge of the colony, a sterile stainless steel punch with a diameter of 0.7 cm was used to make a bacteria-carry...

Embodiment 3

[0072] Under storage conditions, the experiment on the prevention and control effect of Bacillusamyloliquefaciens KB on postharvest diseases of apples and tomatoes:

[0073] 1. After the apple is picked, it is treated with KB bacterial liquid (the concentration of bacterial liquid is 10 6 cfu / ml, 10 7 cfu / ml, 10 8 cfu / ml, 10 9 cfu / ml) soaking for 30-60 seconds, air-dry at room temperature, and then store in the dark at a temperature of 1-2°C and a relative humidity of 90-95%. After storage for 10 months, compared with the control group, when the concentration of the bacterial solution is 10 6 The control effect of cfu / ml is 45%, when the concentration of bacterial solution reaches 10 7 When cfu / ml, its control effect reaches 65%, when the concentration of the bacterial solution reaches 10 8 cfu / ml and 10 9 When cfu / ml, its control effect is 70% and 75% respectively.

[0074] 2. After the tomatoes are picked, they are treated with KB bacteria liquid (the concentration of the bacteria...

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Abstract

The invention discloses Bacillus amyloliquefaciens KB with the collection number of CGMCC No. 8179. The Bacillus amyloliquefaciens KB has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, tomato postharvest botrytis cinerea and apple postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, tomato postharvest gray mold and apple postharvest blue mold. The inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are 22mm and 20mm respectively, and the inhibition rate against peach brown rot spots and apple gray mold spots achieves 100%.

Description

Technical field [0001] A strain of Bacillus amyloliquefaciens which inhibits fungi and its application belong to the technical field of microbial application. Background technique [0002] Plant fungal diseases bring huge losses to agricultural production, and post-harvest fungal diseases are an important type of disease, which caused a large reduction in fruit production, seriously affected the edible value and market value of the fruit, and led to a reduction in the economic income of fruit farmers. In developed countries, the losses caused by diseases after fruit picking accounted for more than 15% of the total fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by diseases after fruit picking accounted for more than 30% of the total. . The occurrence of diseases after fruit picking has severely affected modern agricultural production, especially the production and processing of high-end fruit and ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 张殿朋刘伟成卢彩鸽张涛涛董丹刘霆吴慧玲刘德文卢向阳
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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