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Method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation

A technology of glucose oxidase and microbial fermentation, applied in animal husbandry, application, animal feed, etc., to achieve the effect of good production potential, high enzyme activity and good complementarity

Inactive Publication Date: 2021-05-25
辽宁威兰生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present inventors describe an improved method called Fermentum Spiranoferoxane (FS). They found that certain types of yeast were able to break down proteins like caseinate, carboxysporiellina, lignocystis negotiation mutants, reducing harmful side products from gut cells while improving digestion efficiency. These beneficial properties make these compositions useful in food industry applications including supplementary feeds, diet blenders, sautters, bread fillings, vegetable oil processing, pulp preparations, and others.

Problems solved by technology

Technologies described include methods for producing natural food supplements containing these ingredients through different processes including fermentations, filtrated liquid culture, yeast culturing, laminarocyst clavis preparers, coagulated oligofunction generators, and others. These techniques aim at increasing the efficiency of chemical reactions involved in making edible materials like favagrades, vegetables, bread crops, saututs, strawberrocerways, ruminant sporodes, lycopene, nigrescence bile salt, yarrow texture probes, and liver hydrolysis agents.

Method used

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  • Method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation
  • Method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation
  • Method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] 1. Preparation of Penicillium insolens seed solution:

[0076]1) Determination of enzyme activity of single colony species and first-level solid seed cultivation: pick out 1 / 3 part of one activated Penicillium specificum species, and inoculate it into a 200ml Erlenmeyer flask filled with newly configured Chase culture medium , shake culture in a constant temperature shaker (conditions are 28 ° C, 80 rpm, shaking for 72 hours), measure the enzyme activity of this strain; pick out 2 / 3 parts, and inoculate them into 2 tubes containing the new configuration of specific green In a 200ml eggplant-shaped flat bottle of mold-specific medium (each bottle of medium is 8 grams of millet rice, 2 grams of wheat bran, 20ml of purified water, sterilized), and cultivated in a constant temperature incubator at 28 ° C for 100 hours to obtain specific Penicillium first-grade solid seeds;

[0077] 2) Solid secondary propagation seed culture: 1 bottle of Penicillium inoculum first-level so...

Embodiment 2

[0108] Experiment on Seed Culture Medium and Culture Conditions of Penicillium insolens Fermenter

[0109] 1. Penicillium insolens fermenter seed medium formula test,

[0110] The experimental design is 6 factors and 3 levels. The experimental design and experimental results are shown in Table 1:

[0111] Table 1. Penicillium insolens seed tank fermentation medium formula test

[0112]

[0113]

[0114] 2. Test on culture conditions of Penicillium insolens seed jars

[0115] The experimental design is 5 factors and 3 levels. The experimental design and experimental results are shown in Table 2:

[0116] Table 2. Penicillium insolens seed jar fermentation culture condition test

[0117]

[0118] The Penicillium insolens fermenter seed culture medium determined by the test results of Table 1 and Table 2 is calculated in percentage by weight: sucrose 2.0%, sodium nitrate 0.2%, dipotassium hydrogen phosphate 0.15%, magnesium sulfate 0.15%, potassium chloride 0.2 %, co...

Embodiment 3

[0151] Effect of Different Relative Water Content on Fermentation Temperature in Solid Fermentation

[0152] 1. Experimental design of the influence of different raw material ratios and different water contents on fermentation temperature

[0153] The test design is 3 factors and 9 levels, and the test design and test results are shown in Table 9 below:

[0154] 100-120 parts of fermentation substrate: 20-25 parts of Penicillium insolens seed liquid, 15-20 parts of Bacillus subtilis seed liquid, 5-10 parts of Clostridium butyricum seed liquid, 5-10 parts of Lactobacillus acidophilus seed liquid

[0155] Table 9. Experimental design for the influence of different relative water contents on fermentation temperature in solid fermentation

[0156]

[0157] 2. The test results of the influence of different raw material ratios and different relative water contents on the fermentation temperature. The test results of different raw material ratios and different relative water cont...

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Abstract

The invention relates to a method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation, wherein soybean meal, wheat bran and corn protein powder are used as fermentation raw materials, penicillium notatum is used as a starting strain, and liquid seeds are produced through tube seed and flat solid seed propagation and a one-ton seed tank; the bacillus subtilis, clostridium butyricum and lactobacillus acidophilus are used as starting strains respectively, and propagation is carried out through test tube seeds and shake flask liquid seeds; then a penicillium specific seed solution for generating glucose oxidase, a bacillus subtilis seed solution, a clostridium butyricum seed solution and a lactobacillus acidophilus seed solution for generating active peptides and the fermentation raw materials, which are mixed in sequence before, are mixed according to a ratio, the mixture then is added into a plastic ton bag, a breather pipe is inserted, and a sterile oil-free breather pump is connected; and finally, the functional active peptide powder containing glucose oxidase for livestock and aquatic products produced by using a microbial fermentation method is obtained by carrying out aerobic-anaerobic-aerobic-anaerobic alternate fermentation, drying, crushing, sieving, detecting and the like on the functional active peptide powder containing glucose oxidase.

Description

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Claims

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Application Information

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Owner 辽宁威兰生物技术有限责任公司
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