Method for producing functional active peptide powder containing glucose oxidase for livestock and aquatic products based on microbial fermentation
A technology of glucose oxidase and microbial fermentation, applied in animal husbandry, application, animal feed, etc., to achieve the effect of good production potential, high enzyme activity and good complementarity
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Embodiment 1
[0075] 1. Preparation of Penicillium insolens seed solution:
[0076]1) Determination of enzyme activity of single colony species and first-level solid seed cultivation: pick out 1 / 3 part of one activated Penicillium specificum species, and inoculate it into a 200ml Erlenmeyer flask filled with newly configured Chase culture medium , shake culture in a constant temperature shaker (conditions are 28 ° C, 80 rpm, shaking for 72 hours), measure the enzyme activity of this strain; pick out 2 / 3 parts, and inoculate them into 2 tubes containing the new configuration of specific green In a 200ml eggplant-shaped flat bottle of mold-specific medium (each bottle of medium is 8 grams of millet rice, 2 grams of wheat bran, 20ml of purified water, sterilized), and cultivated in a constant temperature incubator at 28 ° C for 100 hours to obtain specific Penicillium first-grade solid seeds;
[0077] 2) Solid secondary propagation seed culture: 1 bottle of Penicillium inoculum first-level so...
Embodiment 2
[0108] Experiment on Seed Culture Medium and Culture Conditions of Penicillium insolens Fermenter
[0109] 1. Penicillium insolens fermenter seed medium formula test,
[0110] The experimental design is 6 factors and 3 levels. The experimental design and experimental results are shown in Table 1:
[0111] Table 1. Penicillium insolens seed tank fermentation medium formula test
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[0114] 2. Test on culture conditions of Penicillium insolens seed jars
[0115] The experimental design is 5 factors and 3 levels. The experimental design and experimental results are shown in Table 2:
[0116] Table 2. Penicillium insolens seed jar fermentation culture condition test
[0117]
[0118] The Penicillium insolens fermenter seed culture medium determined by the test results of Table 1 and Table 2 is calculated in percentage by weight: sucrose 2.0%, sodium nitrate 0.2%, dipotassium hydrogen phosphate 0.15%, magnesium sulfate 0.15%, potassium chloride 0.2 %, co...
Embodiment 3
[0151] Effect of Different Relative Water Content on Fermentation Temperature in Solid Fermentation
[0152] 1. Experimental design of the influence of different raw material ratios and different water contents on fermentation temperature
[0153] The test design is 3 factors and 9 levels, and the test design and test results are shown in Table 9 below:
[0154] 100-120 parts of fermentation substrate: 20-25 parts of Penicillium insolens seed liquid, 15-20 parts of Bacillus subtilis seed liquid, 5-10 parts of Clostridium butyricum seed liquid, 5-10 parts of Lactobacillus acidophilus seed liquid
[0155] Table 9. Experimental design for the influence of different relative water contents on fermentation temperature in solid fermentation
[0156]
[0157] 2. The test results of the influence of different raw material ratios and different relative water contents on the fermentation temperature. The test results of different raw material ratios and different relative water cont...
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