2014results about How to "Low raw material cost" patented technology

The invention discloses a method of producing bio-diesel by fermenting the heterotrophic chlorella in high density which belongs to the renewable biological energy field. The method uses the heterotrophic chlorella fermented in high density in the bio-reactor as a raw material, which screens the chlorella species having high growth rate and high oil content, directly inoculates into the bottle as the first-class germ to culture then transfers to the fermentation cylinder to undergo the second-glass high density fermentation; the reaction condition is then optimized, and the process is controlled; the culture containing the carbohydrate is dripped to provide the nutrition for the cell growth, therefore the heterotrophic chlorella cells cultured in high density with density of 108g / L and oil content of 61 percent are separated, collected and dried; the algae oil is extracted, and the bio-diesel is produced by transesterification reaction; the invention reduces the cost for the raw material of bio-diesel, and satisfies the demand of industrialized application of producing bio-diesel by heterotrophic chlorella cells, therefore the technique of the invention becomes an economical and effective way of making oils from bio-diesel materials.