Method for producing biodiesel by high-density fermentation of heterotrophic chlorella

Abstract

The invention discloses a method of producing bio-diesel by fermenting the heterotrophic chlorella in high density which belongs to the renewable biological energy field. The method uses the heterotrophic chlorella fermented in high density in the bio-reactor as a raw material, which screens the chlorella species having high growth rate and high oil content, directly inoculates into the bottle as the first-class germ to culture then transfers to the fermentation cylinder to undergo the second-glass high density fermentation; the reaction condition is then optimized, and the process is controlled; the culture containing the carbohydrate is dripped to provide the nutrition for the cell growth, therefore the heterotrophic chlorella cells cultured in high density with density of 108g / L and oil content of 61 percent are separated, collected and dried; the algae oil is extracted, and the bio-diesel is produced by transesterification reaction; the invention reduces the cost for the raw material of bio-diesel, and satisfies the demand of industrialized application of producing bio-diesel by heterotrophic chlorella cells, therefore the technique of the invention becomes an economical and effective way of making oils from bio-diesel materials.

Description

technical field [0001] The invention belongs to the field of renewable bioenergy, and in particular relates to a method for producing biodiesel by high-density fermentation of heterotrophic chlorella. Specifically, a strain of Chlorella protothecoides with high oil content and fast growth is used as the oil-producing algae species, and it is fermented and cultivated at high density in a bioreactor, and the obtained algae oil is then transesterified to prepare biodiesel. Background technique [0002] The increasingly severe environmental pollution and energy shortages in today's world have made human beings face unprecedented challenges. Therefore, people have begun to develop renewable carbon-neutral fuels to replace traditional fossil fuels from the perspective of environmental and economic sustainable development. fuel. Biodiesel is such an environmentally friendly renewable clean energy. Biodiesel refers to the long-chain fatty acid alkyl monoester obtained after esteri...

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Problems solved by technology

Papers published in this research group: (1) Miao XL, Wu QY.2006.Biodiesel production from heterotrophic microalgaloil.Bioresour Technol 97:841-846; (2)Xu H, Miao XL.Wu QY.2006.Highquality biodiesel production from microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 126: 499-507; (3) Xiufeng Li, Han Xu, Qingyu Wu, 2007, Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation. (4): 764-771. And the contents of the applied patents CN1699516A and CN1837352A show that the unit yield of C.protothecoides cells is lower than 16g (dry weight) / L, and the oil content is lower than 56%. Such a low microalgae cell yield and oil content can not meet the requirements of industrial applications

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