Method for producing feeding glucose oxidase by using rice processing by-products as raw materials

Abstract

The invention discloses a method for producing feeding glucose oxidase by using rice processing by-products as raw materials, which comprises the following steps of: (1) inoculation of penicillium notatum of glucose oxidase with high yield into a microorganism culture dish of 10cm filled with a PDA (Potato Dextrose Agar) culture medium for carrying out cultivation; (2) seed cultivation: sterilizing a liquid seed culture medium at a temperature of 115 to 121 DEG C for 20 to 30 min, and then transferring the activated penicillium notatum in the culture dish in the step (1) to the liquid seed culture medium; (3) preparation of a solid fermentation medium: mixing broken rice and rice bran according to the weight ratio of 10: (1-3), and cooking for 25 to 40 min at a temperature of 90 to 100 DEG C; (4) inoculating cultivation: after spreading the solid fermentation medium for cooling, inoculating penicillium notatum seeds for carrying out cultivation; and (5) after ending the solid fermentation, drying the culture medium at a temperature of 100 to 120 DEG C for 2-4h, and crushing to 40 to 80 meshes. The method disclosed by the invention has the advantages that sources of raw materials are wide, no three wastes (waste gas, waste water and industrial residual) are generated, the process is simple and the vigor of the glucose oxidase reaches up to 200 U.

Description

technical field [0001] The invention relates to a production method of glucose oxidase for feed, in particular to a method for producing glucose oxidase for feed by solid fermentation by using broken rice and rice bran as by-products of rice processing. Background technique [0002] At present, the production of glucose oxidase for feed uses the liquid submerged fermentation method, which has many shortcomings in specific applications, for example, the cost of fermentation equipment is high, and liquid submerged fermentation requires professional fermentation equipment, such as fermentation tanks, etc., a 30-ton The cost of the fermentation tank is up to 1 million; the fermentation process consumes a lot of energy and requires constant stirring; the technical quality of the fermentation workers is high; the three wastes (waste water, waste residue, and waste gas) cause serious environmental pollution (Journal of Microbiology 2009, 1 (29 ) p86-89; Food and Fermentation Indust...

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Problems solved by technology

[0002] At present, the production of glucose oxidase for feed uses the liquid submerged fermentation method, which has many shortcomings in specific applications, for example, the cost of fermentation equipment is high, and liquid submerged fermentation requires professional fermentation equipment, such as fermentation tanks, etc., a 30-ton The cost of the fermentation tank is up to 1 million; the fermentation process consumes a lot of energy and requires constant stirring; the technical quality of the fermentation workers is high; the three wastes (waste water, waste residue, and waste gas) cause serious environmental pollution (Journal of Microbiology 2009, 1 (29 ) p86-89; Food and Fermentation Industry 2003, 29 (6) p 87-91)

There is another significant disadvantage of the liquid fermentation method of glucose oxidase for feed production: the raw material used in the fermentation process is mainly high-quality soft white sugar, and the raw material cost is high ("Feed and Animal Husbandry" 2008 (7) p37-39)

Developed countries such as the United States take advantage of their scientific and technological advantages to construct genetically engineered bacteria with high glucose oxidase expression, build liquid fermentation factories in developing countries such as China, and then purchase the original enzyme, which is mainly used in the pharmaceutical and food industries after separation and purification. Some are also used in the feed industry after compounding with other enzyme systems, but there is also the problem of high cost