15 results about "Human ige" patented technology

The present disclosure is directed to human IgE antibodies and fragments thereof binding to galactose-α-1,3-galactose, a known red meat allergen, and methods for use thereof.

The invention discloses an allergen specific IgE and IgG antibody composite quality control product or calibration product as well as a preparation method and application thereof, and belongs to the technical field of biological detection. The preparation method comprises the following steps: obtaining a whole-genome sequence comprising a human IgE constant region gene sequence, a human IgG4 constant region gene sequence and a human IgG1 constant region gene sequence, and preparing a fusion protein simultaneously containing a human IgE Fc segment and a human IgG Fc segment; immunizing a healthy animal by using the allergen to obtain a purified antibody; and carrying out chemical coupling on the purified antibody and the fusion protein to obtain allergen specific IgE and IgG antibodies. By constructing allergen specific IgE and IgG antibodies, the kit can be simultaneously applied to detection of allergens IgE and IgG, has the advantages of high titer, high purity, good dilution linearity and the like, and is beneficial to improvement of diagnosis accuracy and treatment effectiveness.

The invention relates to the technical field of allergy detection, in particular to an inhalation allergen specific IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. The detection objects are pollen, dust mites and animal scurf; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the pollen antigens (Art v1, Amb a1 and Bet v2), the dust mite antigens (Der f1 and Der p1) and the animal scurf antigen (Can f1) are arranged on a nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergen of the inhalation allergy symptom and classifying the severity of the allergy symptom, and the identification result has relatively high accuracy.

The application relates to the field of biotechnology, and in particular to a wheat allergen specific IgE detection kit and a detection method thereof. The kit comprises magnetic particles, enzyme-labeled anti-human IgE antibodies and biotin-labeled wheat allergen. The wheat allergen is obtained by treating a wheat protein extract with heat treatment or a pretreatment agent. The pretreatment agent comprises one or more of sodium azide, L-cysteine and L-cysteine hydrochloride. The wheat allergen is biotin-labeled after specific pretreatment. The chemiluminescence immunoassay method uses streptavidin to prepare magnetic microspheres and horseradish peroxidase-labeled anti-human IgE antibodies. The method is fully automatic, ensures the accuracy of the detection result, and improves the test flux, the specificity and the sensitivity of the detection result.

The invention belongs to the technical field of biological detection, and discloses an allergen-specific IgE antibody detection card, a kit and application. The allergen specificity IgE antibody detection card comprises a combination pad and a nitrocellulose membrane, wherein the combination pad is coated with a mouse anti-human IgE antibody marked by AIE polystyrene fluorescent microspheres and a rabbit IgG antibody marked by AIE polystyrene fluorescent microspheres; a detection line of the nitrocellulose membrane is coated with a specific antigen, and a quality control line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG antibody. The kit prepared from the reagent card is used for detecting the allergen-specific IgE antibody, has the characteristics of high detection sensitivity, high specificity, simplicity in operation, cost saving and the like, and has a very large market and a good application prospect.

The invention relates to the technical field of biological detection, and provides a Janus membrane for penicillin allergy detection. The Janus membrane for penicillin allergy detection is constructed by combining gradient continuous spinning, freezing burying and functionalization treatment, and a blood filtering area and a signal detection area are sequentially arranged from top to bottom. During use, a blood sample firstly enters from the central position of the blood filtering area of the Janus membrane, the blood sample flows into the signal detection area after being filtered, and after antigen penicillin thiazole acid-bovine serum albumin is captured and is combined with an anti-PTAIgE antibody in the blood sample, the colloidal gold labeled anti-human IgE detection probe is used for detection, so that the signal detection area is red. Results show that the kit has the effects of guiding filtered blood samples into a signal detection area, preventing sample leakage, and being high in specificity, convenient to operate, high in detection efficiency, low in false positive rate and visual in result interpretation, and the kit is expected to be widely applied to pre-detection of penicillin allergy conditions in a home first-aid scene due to the fact that the kit is easy and convenient to operate.

The application discloses a centrifugal microfluidic chip for allergen detection, comprising a chip main body and a plurality of microstructures, wherein the chip main body has a rotation center, and the microstructures comprise: a waste chamber located at the farthest end of the rotation center; a reaction detection chamber provided with a carrier, the carrier being coated with anti-human IgE antibodies; a circular truncated cone-shaped channel, the narrow end of which is connected with the other end of the reaction detection chamber, and the wide end of which is connected with a main channel; a serum separation structure comprising a blood cell chamber and a serum chamber, the serum chamber being connected with the main channel through a siphon valve; a first buffer solution chamber; an allergen chamber; a second buffer solution chamber; an enzyme storage chamber; a third buffer solution chamber; a bottom liquid chamber; and a termination liquid chamber. The application avoids contamination and evaporation of reagents, does not need additional driving equipment, is conducive to integration and miniaturization of the equipment, saves consumption of sample whole blood and reagents, reduces cost and time, and is simple to operate; the application needs small amounts of sample and reagents, has low cost, short detection time, and high detection sensitivity.

The present invention relates to the field of antibodies, and particularly to an anti-human IgE antibody and its uses. The CDRs in the heavy chain variable region of the anti-human IgE antibody provided by the present invention include CDR-H1 with an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 7, CDR-H2 with an amino acid sequence as shown in SEQ ID No. 2 or SEQ ID No. 8, and CDR-H3 with an amino acid sequence as shown in SEQ ID No. 3 or SEQ ID No. 9; the CDRs in the light chain variable region of the anti-human IgE antibody include CDR-L1 with an amino acid sequence as shown in SEQ ID No. 4 or SEQ ID No. 10, CDR-L2 with an amino acid sequence as shown in SEQ ID No. 5 or SEQ ID No. 11, and CDR-L3 with an amino acid sequence as shown in SEQ ID No. 6 or SEQ ID No. 12. The antibody provided by the present invention has high affinity and high sensitivity, can detect human IgE at a concentration of 0.02 IU / mL, and has clinical diagnostic significance for identifying allergic reactions.

The present disclosure is directed to a method of detecting a helminth a antigen in a sample comprising (a) contacting a sample with human antibodies and fragments thereof, comprising clone-paired heavy and light chain CDR sequences, and (b) detecting a helminth antigen in said sample by binding of said human antibody or antibody fragment to a helminth antigen in said sample.

The invention relates to the field of biological detection, in particular to a biotinylation-egg white allergen extract, a reagent, a kit and application of the biotinylation-egg white allergen extract. The kit comprises magnetic particles coated with streptavidin, a biotinylation-egg white allergen extract, an anti-human IgE enzyme-labeled antibody, a calibration product and a biotin-labeled anti-human IgE antibody. According to the kit, a streptavidin-biotin system is adopted, so that the loading capacity of a biotinylation-egg white allergen extract is increased; during preparation, the egg white allergen extract is labeled with biotin in different proportions, after labeling with different biotin proportions, reactivity of samples is different, and clinical sensitivity and clinical specificity can be improved by optimizing the biotin proportions. In addition, a surfactant PEG-PPG-PEG is added into the antigen solution, so that the precision of the reagent is improved.

The invention provides an allergen specific antibody detection kit and a preparation method thereof, and belongs to the technical field of in-vitro detection. The allergen specific antibody detection kit comprises a test strip and a separately packaged biotin labeled mouse anti-human IgE, IgG or IgG4 secondary antibody, and a labeled pad in the test strip is loaded with streptavidin labeled receptor microspheres; in the detection pad, a detection line is coated with donor microspheres marked by allergen protein, and a quality control line is coated with donor microspheres marked by goat anti-mouse IgG (Immunoglobulin G). The kit has the characteristics of short detection time, high detection sensitivity, simplicity and convenience in operation and high universality, and can meet the requirements of clinical diagnosis.

The invention discloses a bispecific antibody fusion protein targeting double antigens and application of the bispecific antibody fusion protein. Relates to the technical field of biology. The bispecific antibody fusion protein constructed by the invention can be specifically combined with human IL-5 protein and human IgE protein. Animal experiments show that the fusion protein can significantly reduce the level of key inflammatory factors in an allergic rhinitis model mouse body, effectively relieve nasal symptoms such as sneezing and rhinorrhea, and show a good treatment effect. A novel candidate protein drug is provided for targeted therapy of allergic diseases, especially allergic rhinitis, and the protein has wide development and application prospects and important social value.

The invention relates to the technical field of allergy detection, in particular to an ingestion allergen specificity IgE antibody detection kit and application thereof. The kit is a colloidal gold detection kit. Detection objects are milk, eggs and soybeans; the kit comprises a colloidal gold labeled mouse anti-human IgE antibody arranged on a test strip combination pad; the milk antigens (alpha-La and beta-Lg), the egg antigens (OVA and Gal d1) and the soybean antigens (Gly m2 and Gly m5) are arranged on the nitrocellulose membrane of the test strip. The kit provided by the invention can be used for rapidly identifying the allergens of the ingestion allergy symptoms and classifying the severity of the allergy symptoms, and the identification result has relatively high accuracy.

The invention discloses a Dermatophagoides farinae allergen specificity IgE quantitative detection kit and a preparation method thereof. The Dermatophagoides farinae allergen specificity IgE quantitative detection kit comprises a coating antigen, an anti-human IgE antibody marked by a luminescent substance, and a calibrator, the coating antigen is a biotinylation-dermatophagoides farinae allergen extract and a biotinylation-dermatophagoides farinae allergen component Der f2, wherein the biotinylation-dermatophagoides farinae allergen extract is coated by streptavidin magnetic beads. According to the kit, the mixed antigen is prepared by adopting a special coating process, so that the detection sensitivity is effectively improved, the kit is particularly suitable for detection of low-concentration samples, the specificity and efficiency of antigen reaction are improved, and the accuracy of a detection result is improved.

This invention relates to a fluorescent immunochromatographic test strip for detecting free IgE, comprising a base plate, a sample pad disposed on the base plate, a conjugate pad, a detection membrane, and an absorbent material sequentially overlapped on the base plate, as well as a detection line and a control line disposed on the detection membrane; the fluorescent immunochromatographic test strip for detecting free IgE further includes a filter membrane disposed on the base plate, with one end overlapping the sample pad and the other end overlapping the conjugate pad; the filter membrane is coated with mouse anti-human IgG monoclonal antibody, the conjugate pad is coated with fluorescent microsphere-labeled FcERI antigen and fluorescent microsphere-labeled DNP-BSA, the detection line is coated with mouse anti-human IgE antibody, and the control line is coated with DNP monoclonal antibody. This fluorescent immunochromatographic test strip has advantages such as high sensitivity, high accuracy, good stability, good repeatability, and ease of operation, and has broad application prospects.