Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection
A technology of serum immunity and quantitative detection, which is applied in the field of clinical medical examination, can solve the problem that differential diagnosis has no practical significance, and achieve the effect of preventing specimen error, simple operation, and low detection cost
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[0039] 1. Preparation of colloidal gold-labeled mouse anti-human IgE monoclonal antibody
[0040] Raw materials: mouse anti-human IgE monoclonal antibody, commercial reagents, products of ABCAM Company in the United Kingdom. Colloidal gold particles, commercial reagents, products of Zhongxin Keju Biotechnology Company.
[0041] method:
[0042] with 0.1M K 2 CO 3 Or 0.1M HCl to adjust the pH of the colloidal gold solution to about 8.0-8.4; add the antibody (0.6 mg of antibody) to 20 ml of the colloidal gold solution (the particle size of the colloidal gold particle is 41nm), and stir for 5-10 minutes; then add 100mg / mL bovine serum albumin (BSA), until the final concentration of BSA is 5mg / mL, stir and mix for 15min; then add 200mg / mL PEG20000 (polyethylene glycol, molecular weight 20,000) until the final concentration of PEG 20000 is 2mg / mL , and then continue to stir and mix for 15 minutes; stand at 4°C overnight; centrifuge at 14,000 rpm for 30 minutes, carefully discar...
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