Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection

A technology of serum immunity and quantitative detection, which is applied in the field of clinical medical examination, can solve the problem that differential diagnosis has no practical significance, and achieve the effect of preventing specimen error, simple operation, and low detection cost

Inactive Publication Date: 2017-02-01
天津中新科炬生物制药股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

For abnormal specimens, accurate quantification to the "ten" place has no practical significance for clinical diagnosis, especially differential diagnosis

Method used

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  • Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection
  • Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection
  • Kit and test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Preparation of colloidal gold-labeled mouse anti-human IgE monoclonal antibody

[0040] Raw materials: mouse anti-human IgE monoclonal antibody, commercial reagents, products of ABCAM Company in the United Kingdom. Colloidal gold particles, commercial reagents, products of Zhongxin Keju Biotechnology Company.

[0041] method:

[0042] with 0.1M K 2 CO 3 Or 0.1M HCl to adjust the pH of the colloidal gold solution to about 8.0-8.4; add the antibody (0.6 mg of antibody) to 20 ml of the colloidal gold solution (the particle size of the colloidal gold particle is 41nm), and stir for 5-10 minutes; then add 100mg / mL bovine serum albumin (BSA), until the final concentration of BSA is 5mg / mL, stir and mix for 15min; then add 200mg / mL PEG20000 (polyethylene glycol, molecular weight 20,000) until the final concentration of PEG 20000 is 2mg / mL , and then continue to stir and mix for 15 minutes; stand at 4°C overnight; centrifuge at 14,000 rpm for 30 minutes, carefully discar...

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Abstract

The invention provides a kit and a test paper strip for serum immunoglobulin E colloidal gold chromatography quantitative detection. The kit includes: a colloidal gold marked mouse-anti-human IgE monoclonal antibody, a rabbit-anti-human IgE polyclonal antibody, and a goat-anti-mouse IgG antibody. In the invention, the colloidal gold immunochromatography technology is employed, and the kit and the test paper strip need to be combined with an immunochromatography result interpretoscope. The kit and the test paper strip are easy to use, are free of training and can be rapidly learnt, are free of large-size devices and are suitable for primary laboratories or community laboratories.

Description

technical field [0001] The invention belongs to the field of clinical medical testing, in particular to a colloidal gold chromatography rapid quantitative detection kit for serum immunoglobulin E, which adopts colloidal gold immune chromatography technology and is used in combination with an immune chromatography result interpreter. Background technique [0002] "Allergic disease" refers to a pathological immune response that occurs when the body is stimulated by the same antigen again after the body responds to certain antigens for the first time. Allergic diseases are mainly mediated by immunoglobulin E (IgE), and the antigens that cause allergic diseases are called "allergens". Allergens can stimulate B lymphocytes to produce specific IgE (sIgE) antibodies after entering the body, and sIgE combines with mast cells or basophils to put the body in an sensitized state. When the same allergen enters the sensitized body again, the allergen combines with specific IgE antibodie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/531
CPCG01N33/6854G01N33/531G01N33/558G01N33/577
Inventor 李会强周洪锐常艳敏李洲
Owner 天津中新科炬生物制药股份有限公司
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