42 results about "Serum specific ige" patented technology

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result. The invention also provides monoclonal antibodies against WNV NS5 and DENV NS5 antigen and their use in detecting WNV and DENV infections in a biological sample.

The invention provides a test strip and a kit for detecting a human novel coronavirus IgM antibody and a preparation method of the test strip and the kit. A nitrocellulose membrane of the test strip is provided with a detection line and a quality control line; the detection line is coated with novel coronavirus nucleocapsid protein and S1 protein, and the quality control line is coated with a chicken IgY antibody; the combination pad of the test strip is coated with a quantum dot labeled goat anti-human IgM antibody and a quantum dot labeled goat anti-chicken IgY antibody. According to the test strip provided by the invention, novel coronavirus N protein and S1 protein are simultaneously coated on the detection line; the goat anti-human IgM antibody and the goat anti-chicken IgY antibody are labeled by using quantum dots; the human IgM antibody is a serum specific antibody firstly appearing after being infected with novel coronavirus; and the obtained test strip can be used for effectively and accurately detecting patients infected with the novel coronavirus at the early stage, and has important significance for preventing further diffusion of the virus.

The invention discloses a pORF65 recombinant protein and a preparation method and application thereof. The pORF65 recombinant protein comprises amino acid sequences shown in the formulas of SEQ ID NO.17 to SEQ ID NO.21 orderly connected by connexins, and the connexin is composed of 4-7 hydrophilic amino acids with small molecular weight and polarity. The ELISA result shows that the pORF65 recombinant protein can be used as a coating antigen and can well detect a serum-specific antibody produced by a cryprinus carpiod immunized by a DNA vaccine constructed from a complete coding sequence of CyHV-3 pORF65 and diagnose whether the cryprinus carpiod infects cyprinid herpesvirus III.

The invention discloses lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE, and belongs to the technical field of microorganisms and the technical field of medicines. The invention provides lactobacillus reuteri CCFM1190. The lactobacillus reuteri CCFM1190 can be used for relieving the pathological characteristics of mice with food allergy, so that the lactobacillus reuteri CCFM1190 which can further relieve food allergy has the specific advantages that the OVA specific IgE level of the serum of the mouse with the food allergy is reduced, the weight loss of the serum of the mouse with the food allergy is relieved, the lesion degree of the serum jejunum tissue of the mouse with the food allergy is relieved, and the allergic symptoms of the mouse with the food allergy are relieved, so that the lactobacillus reuteri CCFM1190 can be used for preparing products for preventing and / or treating food allergy such as food, medicine or health food and the like, andhas a huge application prospect.

The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

The invention discloses a kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution, and belongs to the field of serum biological markers. The kit includes 3 PCR primers for serum miRNA. The kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution provided by the present invention adopts three serum miRAs as diagnostic markers, and quantifies the above markers to identify Yin deficiency and inflamed constitution, which can replace the traditional The TCM diagnostic methods of smelling, asking, and cutting combine the diagnosis of TCM syndromes with modern molecular biology to achieve the standardization of diagnosis; and the kit for detecting serum-specific miRNAs of Yin deficiency and inflamed constitution, through the Three serum miRNAs were quantified at the same time to identify Yin-deficiency inflamed constitution. The diagnostic sensitivity reached 95.7% and the specificity reached 73.6%, which was higher than the diagnostic characteristics of a single miRNA.

A novel K (capsular antigen) serotype of Vibrio parahaemolyticus and an application thereof are provided. A novel K (capsular antigen) serotype of Vibrio parahaemolyticus, which was deposited at the China General Microbiological Culture Collection Center (Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing) on Feb. 20, 2019, with a deposit number of CGMCC No. 17249, wherein the K epitope of the Vibrio parahaemolyticus has a sequence set forth in Sequence No. 1. The novel K serum is highly specific, and can be used to conveniently and quickly detect a novel K serotype of Vibrio parahaemolyticus (O4:KUT-recAin) which has a rising infection rate in recent years. It provides important detection techniques for the pathogen diagnosis, monitoring and prevention of infectious diarrhea.

A serum-specific biomarker for the diagnosis of primary liver cancer and its screening method and application, using the Boyden chamber to separate the protrusion and cell body of liver cancer metastatic cells, starting from the subcellular structure, using the improved Boyden chamber method to separate liver cancer metastatic cells For cell bodies and protrusions, the separation results were verified by Western-blot, RNA staining, immunofluorescence and other techniques. Study the differential expression in HCCLM3 liver cancer cell body and protrusion, and screen out microRNAs related to the invasion and metastasis of HCC. Clinical experiments have proved that the change of individual serum hsa-let-7c-3p has a higher diagnostic value for HCC. Sensitivity and specificity. The present invention will provide a new diagnostic method for clinical diagnosis of primary liver cancer, and has certain potential clinical value in assisting the treatment of HCC and monitoring the course of disease, through the ROC curve of let-7c-3p, and let-7c-3p The ROC curves of combined use with CEA, let-7c-3p with AFP, and let-7c-3p with AFP and CEA have improved sensitivity and specificity, and the diagnostic value has been significantly improved.

The invention discloses pORF65 recombinant protein and its preparation method and application. The pORF65 recombinant protein is sequentially connected by amino acid sequences shown in SEQ ID NO.17-SEQ ID NO.21 through a linker, and the linker is 4- Consists of 7 small molecular weight, polar and hydrophilic amino acids. Shown by ELISA, the pORF65 recombinant protein of the present invention can be used as a coating antigen, and can be well used to detect the serum-specific antibodies produced after the DNA vaccine immunized Koi with the complete coding sequence of CyHV-3 pORF65, and to diagnose Koi Whether infected with carp herpesvirus type 3.

The present invention relates to the application of miR-122-5p, miR-483-5p and miR-3197 in combination as molecular markers for getting angry in traditional Chinese medicine; it involves the combined use of miR-122-5p, miR-483-5p, miR-3197 and U6 Application in the preparation of traditional Chinese medicine miRNA detection kits; the present invention uses serum hsa-miR-122-5p, hsa-miR-483-5p and hsa-miR-3197 as diagnostic markers, verified by large samples, in practice In people with heat and fire, individual serum hsa-miR-122-5p, hsa-miR-483-5p or hsa-miR-3197 all have significantly low expression, and the kit provided by the invention is used to detect the constitution of excessive heat and fire , through simultaneous relative quantification of serum hsa‑miR‑122‑5p, hsa‑miR‑483‑5p and hsa‑miR‑3197 to identify excess heat and upper body fire constitution, the diagnostic sensitivity is 90.9%, and the specificity is 72.7% , has high accuracy and is higher than the diagnostic properties of a single miRNA. It can replace the traditional TCM diagnostic methods of looking, smelling, asking, and feeling, and combines the diagnosis of TCM syndromes with modern molecular biology to achieve standardization of diagnosis.