42 results about "Serum specific ige" patented technology

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result. The invention also provides monoclonal antibodies against WNV NS5 and DENV NS5 antigen and their use in detecting WNV and DENV infections in a biological sample.

The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

The invention discloses a kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution, and belongs to the field of serum biological markers. The kit includes 3 PCR primers for serum miRNA. The kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution provided by the present invention adopts three serum miRAs as diagnostic markers, and quantifies the above markers to identify Yin deficiency and inflamed constitution, which can replace the traditional The TCM diagnostic methods of smelling, asking, and cutting combine the diagnosis of TCM syndromes with modern molecular biology to achieve the standardization of diagnosis; and the kit for detecting serum-specific miRNAs of Yin deficiency and inflamed constitution, through the Three serum miRNAs were quantified at the same time to identify Yin-deficiency inflamed constitution. The diagnostic sensitivity reached 95.7% and the specificity reached 73.6%, which was higher than the diagnostic characteristics of a single miRNA.

A novel K (capsular antigen) serotype of Vibrio parahaemolyticus and an application thereof are provided. A novel K (capsular antigen) serotype of Vibrio parahaemolyticus, which was deposited at the China General Microbiological Culture Collection Center (Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing) on Feb. 20, 2019, with a deposit number of CGMCC No. 17249, wherein the K epitope of the Vibrio parahaemolyticus has a sequence set forth in Sequence No. 1. The novel K serum is highly specific, and can be used to conveniently and quickly detect a novel K serotype of Vibrio parahaemolyticus (O4:KUT-recAin) which has a rising infection rate in recent years. It provides important detection techniques for the pathogen diagnosis, monitoring and prevention of infectious diarrhea.

The invention discloses pORF65 recombinant protein and its preparation method and application. The pORF65 recombinant protein is sequentially connected by amino acid sequences shown in SEQ ID NO.17-SEQ ID NO.21 through a linker, and the linker is 4- Consists of 7 small molecular weight, polar and hydrophilic amino acids. Shown by ELISA, the pORF65 recombinant protein of the present invention can be used as a coating antigen, and can be well used to detect the serum-specific antibodies produced after the DNA vaccine immunized Koi with the complete coding sequence of CyHV-3 pORF65, and to diagnose Koi Whether infected with carp herpesvirus type 3.

The present invention relates to the application of miR-122-5p, miR-483-5p and miR-3197 in combination as molecular markers for getting angry in traditional Chinese medicine; it involves the combined use of miR-122-5p, miR-483-5p, miR-3197 and U6 Application in the preparation of traditional Chinese medicine miRNA detection kits; the present invention uses serum hsa-miR-122-5p, hsa-miR-483-5p and hsa-miR-3197 as diagnostic markers, verified by large samples, in practice In people with heat and fire, individual serum hsa-miR-122-5p, hsa-miR-483-5p or hsa-miR-3197 all have significantly low expression, and the kit provided by the invention is used to detect the constitution of excessive heat and fire , through simultaneous relative quantification of serum hsa‑miR‑122‑5p, hsa‑miR‑483‑5p and hsa‑miR‑3197 to identify excess heat and upper body fire constitution, the diagnostic sensitivity is 90.9%, and the specificity is 72.7% , has high accuracy and is higher than the diagnostic properties of a single miRNA. It can replace the traditional TCM diagnostic methods of looking, smelling, asking, and feeling, and combines the diagnosis of TCM syndromes with modern molecular biology to achieve standardization of diagnosis.