42 results about "Serum specific ige" patented technology

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result.

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result. The invention also provides monoclonal antibodies against WNV NS5 and DENV NS5 antigen and their use in detecting WNV and DENV infections in a biological sample.

The invention provides a light-activating chemiluminescence immunity analysis kit for serum specific IgE (immunoglobulin E). The kit adopts a luminous microsphere coated with a specific single-component allergen (prepared by naturally purification or recombination), a biotin-labeled anti-human-IgE antibody and a light-sensitive microsphere precoated with streptavidin to form detecting reagents. Firstly, to-be-detected serum, the luminous microsphere coated the allergen and the a biotin-labeled anti-human-IgE antibody are incubated together, and the specific IgE antibody in the to-be-detected serum is bound with an antigen molecule on the surface of the luminous microsphere and the biotin-labeled anti-human-IgE antibody respectively; secondly, the light-sensitive microsphere precoated with streptavidin is added for continuous incubation, and is bridged with the luminous microsphere through the binding of biotin and streptavidin; thirdly, a light-activating chemiluminescence analyzing instrument is used for detecting the strength of light signals. The kit has various advantages of being easy and quick to operate, facilitating automation and quantitative analysis, and the like.

The invention provides a test strip and a kit for detecting a human novel coronavirus IgM antibody and a preparation method of the test strip and the kit. A nitrocellulose membrane of the test strip is provided with a detection line and a quality control line; the detection line is coated with novel coronavirus nucleocapsid protein and S1 protein, and the quality control line is coated with a chicken IgY antibody; the combination pad of the test strip is coated with a quantum dot labeled goat anti-human IgM antibody and a quantum dot labeled goat anti-chicken IgY antibody. According to the test strip provided by the invention, novel coronavirus N protein and S1 protein are simultaneously coated on the detection line; the goat anti-human IgM antibody and the goat anti-chicken IgY antibody are labeled by using quantum dots; the human IgM antibody is a serum specific antibody firstly appearing after being infected with novel coronavirus; and the obtained test strip can be used for effectively and accurately detecting patients infected with the novel coronavirus at the early stage, and has important significance for preventing further diffusion of the virus.

The invention relates to the technical field of biology, in particular to streptococcus pneumonia fusion protein and a vaccine thereof. The fusion protein is an expression product formed by recombination of a streptococcus pneumonia virulence protein gene and a protein connector gene, and streptococcus pneumonia virulence protein is Ply or PspA or PsaA or PcpA or PhtD. After the vaccine of the streptococcus pneumonia fusion protein immunizes an animal through the nasal cavity, a high-potency serum specific IgG antibody can be induced, high-potency local mucosa specific IgA can be induced, and a good protective effect is achieved for nasal cavity toxin counteracting of different streptococcus pneumonia strains.

The invention tests liver protection and immune regulation functions by feeding a rat and a mouse with an antradiacomphora capsule which is prepared by adopting a special process and solid-state culture, wherein a liver protection test comprises the following steps of: inducing chronic hepatitis of the rat by using carbon tetrachloride twice a week for eight weeks, and measuring GPT and GOT, water content of liver, spleen and liver, liver protein and hydroxyproline content. The antradiacomphora capsule can lighten the rat chronic hepatitis induced by the carbon tetrachloride. A specific immunity test: the test matter is fed to the BALB / c mouse and egg albumen is injected, thereby accelerating the immune cell proliferation capacity and the effect of the immune regulation function generated by an antibody with serological specificity. A non-specific immunity test comprises the following step of: doing an oral test for consecutive six weeks by means of the BALB / c mouse. The invention accelerates the abdominal cavity phagocyte activity, regulates the anti-tumor cytohormone secretion and promotes the effect of the immune regulation function generated by a serological antibody.

The invention uses serum specificity protein group fingerprint diagram to diagnose carcinoma of colon and rectal carcinoma in early stage, the invention produces the protein group template at first, selects the outer circle blood of confirmed sufferers, benign sufferer and normal people. Separates the serum in 4 degrees centigrade cold-storage, absorbs the serum centrifugally, packages, backup in subzero 80 degrees centigrade; when in detection, it is diluted according to a density; uses the metal chip of America saifuji corporation; sets in the fly mass spectrum device of America safifuji corporation, analyzes the protein group wave spectrum of the sample serum; compares and analyzes the serum protein group wave spectrum of the carcinoma of colon and rectal carcinoma sufferers, the benign sufferer and health people, acquires the special protein peak in the serum; forms the protein template by combining protein peak in serum, uses the template double-blind method to carry on clinic sifting experiment, confirms the positive predicting valve, negative predicting valve, sensitivity and specificity of the fingerprint diagram.

The invention belongs to the technical field of medicaments and in particular relates to mycobacterium tuberculosis antibody binding peptide and application thereof. The general formula of an amino acid sequence is X1X2X3PPFS, wherein P represents proline; F represents phenylalanine; S represents serine; X1 to X3 represent any amino acid; preferably, X1 is L or F, X2 is P or H, and X3 is P or F; and L represents leucine and H represents histidine. The tuberculosis patient serum specific antibody-bound polypeptide can be used as a detection reagent in tuberculosis serological detection.

The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of Torque teno sus virus (TTsuV). The present invention also provides methods for diagnosing TTsuV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies which indicate infections TTsuV1, TTsuV2, and individual TTsuV1 genotypes.

The invention relates to polypeptide biological technical field, which discloses a spatial conformation mimic antigen epitope of H3-type influenza virus hemagglutinin, and relative application. The sequence of the spatial conformation mimic antigen epitope is SEQ ID No. 1. The invention utilizes anti-recombination HA1 rabbit serum IgG selection phage 12 peptide library, uses phage ELISA, DNA sequencing, western bolt, and software analysis to analyze selected colony, and selects the small peptide from random phage 12 peptide library, which can be specially combined with anti-recombination HA1 rabbit serum IgG. The inventive spatial conformation mimic antigen epitope can prepare diagnostic reagent box of H3-type influenza virus.

The invention relates to a method for detecting the effectiveness of aftosa inactivated vaccines. The method comprises the following steps that firstly, each dose of each batch of vaccines immunizes 4 to 8 Balb / C series rats, the immunizing dose of the vaccines can be classified into 1 rat / part, one-second rat / part and one-fourth rat / part, blood sampling is performed on the twenty-eighth day, and serum is separated; secondly, serum specific antibodies are detected through an aftosa inhibiting ELISA method, and if antibody inhibiting ELISA titer-log10X is higher than 2.0 and variable coefficient is lower than 12 percent, the antibodies are judged to be qualified. The invention provides a novel method for detecting the effectiveness of aftosa inactivated vaccine, immunizing Balb / C series rats and detecting serum through the method can properly reflect the effective antigen content in the vaccines, and the method replaces the original animal immunization challenge test.

The invention discloses H5 subtype avian influenza virus HA protein B cell epitope polypeptide and application thereof. An H5 subtype AIV (avian influenza virus) HA amino acid sequence is used as the template, the epitope polypeptide is synthesized on a solid-phase carrier in situ, each polypeptide contains 15 amino acids, and 14 amino acids of each previous polypeptide and the corresponding next polypeptide overlap. The synthesized H5 AIV HA epitope is allowed to have reaction with known H5 subtype positive serum, and one epitope polypeptide (H5-1) which can have specific reaction with H5 AIV chicken serum. The epitope polypeptide is used for detecting H5 subtype avian influenza viruses, and the detection result shows that the ELISA method built by the epitope polypeptide is suitable for detecting a 1:200 diluted serum sample. A coincidence rate test proves that the detection result of epitope polypeptide on H5 AIV negative serum is negative, the detection coincidence rate of samples with the hemagglutination inhibition values ranging from log2 to log4 is 87.6%, and the detection coincidence rate of samples with the hemagglutination inhibition values being log5 or above log5 is 98.3%.

The invention provides application of a QX type IBV cellular adapted strain MJ to preparation of a totivirus antigen ELISA inspection kit and the kit, and belongs to the technical field of virus antibody inspection. According to the application of the QX type IBV cellular adapted strain MJ to the preparation of the totivirus antigen ELISA inspection kit, the preservation number of the QX type IBVcellular adapted strain MJ is CGMCC No. 14681, the cellular adapted strain MJ does not contain other viral antigens and is only in specific binding with an IBV positive serum, and cross reaction between the cellular adapted strain MJ and other virus positive serums is avoided, effective proliferation is conducted on a Vero cell, and the cellular adapted strain MJ is applied to effectively solve the problems that purification of IBV totivirus is difficult and non-specificity is high. The invention also provides the totivirus antigen indirect ELISA inspection reagent box constructed based on thecellular adaptive strain MJ. According to the totivirus antigen indirect ELISA inspection kit, detection sensitivity is high, specificity is strong, QX type IBV early infection can be inspected, andearly antibody level of chicken flock immune from attenuated vaccine can also be inspected.

The invention discloses a pORF65 recombinant protein and a preparation method and application thereof. The pORF65 recombinant protein comprises amino acid sequences shown in the formulas of SEQ ID NO.17 to SEQ ID NO.21 orderly connected by connexins, and the connexin is composed of 4-7 hydrophilic amino acids with small molecular weight and polarity. The ELISA result shows that the pORF65 recombinant protein can be used as a coating antigen and can well detect a serum-specific antibody produced by a cryprinus carpiod immunized by a DNA vaccine constructed from a complete coding sequence of CyHV-3 pORF65 and diagnose whether the cryprinus carpiod infects cyprinid herpesvirus III.

The invention discloses lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE, and belongs to the technical field of microorganisms and the technical field of medicines. The invention provides lactobacillus reuteri CCFM1190. The lactobacillus reuteri CCFM1190 can be used for relieving the pathological characteristics of mice with food allergy, so that the lactobacillus reuteri CCFM1190 which can further relieve food allergy has the specific advantages that the OVA specific IgE level of the serum of the mouse with the food allergy is reduced, the weight loss of the serum of the mouse with the food allergy is relieved, the lesion degree of the serum jejunum tissue of the mouse with the food allergy is relieved, and the allergic symptoms of the mouse with the food allergy are relieved, so that the lactobacillus reuteri CCFM1190 can be used for preparing products for preventing and / or treating food allergy such as food, medicine or health food and the like, andhas a huge application prospect.

The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

The invention discloses a method for detecting aortic coarctation markers based on proteomics, and belongs to the technical field of protein detection. The method comprises the following steps of sample protein extraction, protein quantification, proteolysis, isobaric tags for relative and absolute quantitation (iTRAQ) marking, protein analysis, result detecting and follow-up verification. According to the method for detecting the aortic coarctation markers based on proteomics, a serum specific differential protein of child patients with aortic coarctation is clarified, the protein markers with good sensitivity and high specificity in clinical aortic coarctation laboratory examination are prepared, thus the purpose of decreasing the misdiagnosis rate at the early stage of the disease course of the child patients with aortic coarctation is achieved, the misdiagnosis rate at the early stage of the disease course of the child patients with aortic coarctation can be decreased by combiningconventional echocardiographic examination at the present stage, and import significance is achieved in clinical practical application.

An oral transenterocytotic mucosal adhesion vehicle includes a ghost cell formed from fermented bacterium providing a container for uptake and active transport via a lacteal surface and a nano encapsulated drug particle including a bioactive agent which is encapsulated by a polymeric coating disposed within said ghost cell and surviving first pass liver metabolism, wherein the polymeric coating comprises molecules of an alginate and transmucosal delivery enhancing molecules, wherein the transmucosal delivery enhancing molecules are covalently conjugated to the alginate molecules, wherein the polymeric delivery vehicle is resistant to intestinal degradation and wherein the polymeric delivery vehicle is capable of transmucosal passage across the intestinal mucosa into the lymphatic capillary wherein the polymeric delivery vehicle comprising the alginate molecules and transmucosal delivery enhancing molecules covalently conjugated thereto is degraded to release substantially all of the bioactive agent.

A novel K (capsular antigen) serotype of Vibrio parahaemolyticus and an application thereof are provided. A novel K (capsular antigen) serotype of Vibrio parahaemolyticus, which was deposited at the China General Microbiological Culture Collection Center (Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing) on Feb. 20, 2019, with a deposit number of CGMCC No. 17249, wherein the K epitope of the Vibrio parahaemolyticus has a sequence set forth in Sequence No. 1. The novel K serum is highly specific, and can be used to conveniently and quickly detect a novel K serotype of Vibrio parahaemolyticus (O4:KUT-recAin) which has a rising infection rate in recent years. It provides important detection techniques for the pathogen diagnosis, monitoring and prevention of infectious diarrhea.

The invention discloses a kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution, and belongs to the field of serum biological markers. The kit includes 3 PCR primers for serum miRNA. The kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution provided by the present invention adopts three serum miRAs as diagnostic markers, and quantifies the above markers to identify Yin deficiency and inflamed constitution, which can replace the traditional The TCM diagnostic methods of smelling, asking, and cutting combine the diagnosis of TCM syndromes with modern molecular biology to achieve the standardization of diagnosis; and the kit for detecting serum-specific miRNAs of Yin deficiency and inflamed constitution, through the Three serum miRNAs were quantified at the same time to identify Yin-deficiency inflamed constitution. The diagnostic sensitivity reached 95.7% and the specificity reached 73.6%, which was higher than the diagnostic characteristics of a single miRNA.

Early Prostate Cancer Antigen-2 (EPCA-2) is a prostate cancer biomarker, newly identified via proteomics-based examination of alterations in nuclear structure. EPCA-2 shows a higher overall specificity than PSA. Thus, an immunoassay based on antibodies against an epitope of EPCA-2 more accurately differentiates between confined and non-confined disease than PSA or Gleason score.

The invention belongs to the technical field of biomedicine and clinical application, relates to a polypeptide fragment specifically binding to the serum of Babesiosis patients, and an amino acid sequence thereof. According to the present invention, a series polypeptide fragments are obtained by analyzing the structure and the epitope of a Babesia merozoite surface protein BMASA, and the polypeptide fragment is prepared through polypeptide synthesis, and can be used for preparing the serological immunodiagnostic reagent for Babesiosis; and with the polypeptide fragment, the polypide missing diagnosis and the polypide misdiagnosis of the blood smear in the diagnosis of Babesiosis can be overcome, and the specificity and the sensitivity of the diagnosis of Babesiosis can be significantly improved.

The invention relates to a polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and a preparation method thereof. Prokaryotic expression plasmids of the porcine epidemic diarrhea virus Nsp15 protein are successfully constructed, 37 DEG C is found to be the optimal induction temperature, the high-purity Nsp15 protein is obtained through nickel column purification, the purity is as high as 90%, the protein concentration is 0.69 mg / mL, then the Nsp15 protein polyclonal antibody with high titer is prepared, the titer of the antibody is 2 * 10 < 5 >, a Western Blot test verifies that Nsp15 positive serum specificity is good, and the Nsp15 protein polyclonal antibody can be used for preparing the porcine epidemic diarrhea virus Nsp15 protein polyclonal antibody. The kit can be used for detecting the porcine epidemic diarrhea virus and researching the functional mechanism of Nsp15.

The present invention discloses specific antibody of humphead snapper and its preparation process. The pecific antibody of humphead snapper as high-tech bioengineering product extracted from sea water body includes a serum IgM, a specific yolk antibody and a fish fry antibody with high purity and high specificity. The preparation process includes immunizing sexually matured female humphead snapper with commercial goat IgG, taking parent fish's serum and yolk, promoting propagation, taking fish fry and homogenizing, stepped salting out with saturated ammonium sulfate solution and affinity chromatography to purify the specific antibody. The present invention relates to immunological technology for preventing and treating diseases of aquatic animal, and is significant in raising the disease resistance and survival rate of humphead snapper fry.

A serum-specific biomarker for the diagnosis of primary liver cancer and its screening method and application, using the Boyden chamber to separate the protrusion and cell body of liver cancer metastatic cells, starting from the subcellular structure, using the improved Boyden chamber method to separate liver cancer metastatic cells For cell bodies and protrusions, the separation results were verified by Western-blot, RNA staining, immunofluorescence and other techniques. Study the differential expression in HCCLM3 liver cancer cell body and protrusion, and screen out microRNAs related to the invasion and metastasis of HCC. Clinical experiments have proved that the change of individual serum hsa-let-7c-3p has a higher diagnostic value for HCC. Sensitivity and specificity. The present invention will provide a new diagnostic method for clinical diagnosis of primary liver cancer, and has certain potential clinical value in assisting the treatment of HCC and monitoring the course of disease, through the ROC curve of let-7c-3p, and let-7c-3p The ROC curves of combined use with CEA, let-7c-3p with AFP, and let-7c-3p with AFP and CEA have improved sensitivity and specificity, and the diagnostic value has been significantly improved.

The invention relates to a serum specific biomarker used for diagnosing primary liver cancer and a screening method and an application thereof. According to the invention, a Boyden chamber is used forseparating extension and soma of liver cancer transfer cells, from a subcellular structure, an improved Boyden chamber method is used for separating extension and soma of liver cancer transfer cells,and the separating results can be verified by using the technologies such as Western-blot, RNA dyeing and immunofluorescence. The differential expression condition in HCCLM3 liver cancer cell extension and soma is researched, minimal RNA-related to a HCC infiltration transferring process is screened, and clinical experiment proves that change of individual serum has-let-7c-3p has high sensitivityand specificity on HCC diagnosis. The serum specific biomarker provides a novel diagnosis means for clinical diagnosis of the primary liver cancer, and has certain latent clinical value on HCC treatment and pathogenesis monitoring, through a ROC curve of let-7c-3p as well as the ROC curve of let-7c-3p and CEA combination, let-7c-3p and AFP combination, and let-7c-3p and AFP and CEA combination, sensitivity and specificity are increased, and diagnosis value can be obviously increased.

The present invention relates to the application of miR-122-5p, miR-483-5p and miR-3197 in combination as molecular markers for getting angry in traditional Chinese medicine; it involves the combined use of miR-122-5p, miR-483-5p, miR-3197 and U6 Application in the preparation of traditional Chinese medicine miRNA detection kits; the present invention uses serum hsa-miR-122-5p, hsa-miR-483-5p and hsa-miR-3197 as diagnostic markers, verified by large samples, in practice In people with heat and fire, individual serum hsa-miR-122-5p, hsa-miR-483-5p or hsa-miR-3197 all have significantly low expression, and the kit provided by the invention is used to detect the constitution of excessive heat and fire , through simultaneous relative quantification of serum hsa‑miR‑122‑5p, hsa‑miR‑483‑5p and hsa‑miR‑3197 to identify excess heat and upper body fire constitution, the diagnostic sensitivity is 90.9%, and the specificity is 72.7% , has high accuracy and is higher than the diagnostic properties of a single miRNA. It can replace the traditional TCM diagnostic methods of looking, smelling, asking, and feeling, and combines the diagnosis of TCM syndromes with modern molecular biology to achieve standardization of diagnosis.