Colorectal cancer early diagnosis utilizing serological specificity protein group finger print

Abstract

The invention uses serum specificity protein group fingerprint diagram to diagnose carcinoma of colon and rectal carcinoma in early stage, the invention produces the protein group template at first, selects the outer circle blood of confirmed sufferers, benign sufferer and normal people. Separates the serum in 4 degrees centigrade cold-storage, absorbs the serum centrifugally, packages, backup in subzero 80 degrees centigrade; when in detection, it is diluted according to a density; uses the metal chip of America saifuji corporation; sets in the fly mass spectrum device of America safifuji corporation, analyzes the protein group wave spectrum of the sample serum; compares and analyzes the serum protein group wave spectrum of the carcinoma of colon and rectal carcinoma sufferers, the benign sufferer and health people, acquires the special protein peak in the serum; forms the protein template by combining protein peak in serum, uses the template double-blind method to carry on clinic sifting experiment, confirms the positive predicting valve, negative predicting valve, sensitivity and specificity of the fingerprint diagram.

Description

Technical field: [0001] The invention relates to the field of bioengineering, and relates to the early diagnosis of colorectal cancer by using serum-specific proteome fingerprints. Background technique: [0002] With the completion of most of the sequencing work of the human genome, the gene framework has been drawn. However, the function of the gene is not only determined by its own sequence, but also gives life a lot of information and wide variation, making the disease mechanism an extremely important issue. However, the ultimate executor of gene function is protein. Therefore, studying the structure and properties of protein is the most critical link in revealing the chain of genes and diseases. Previous studies on genomics have found some genes that are closely related to the formation and development of colorectal cancer, such as ras, p53, p16, nm23, etc., among which the overexpression of ras gene and the deletion of p16 gene can be used as important indicators for th...

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Problems solved by technology

[0006] 1. Stool examination: The commonly used multi-step enzyme chain reaction-single-strand conformation polymorphism (PCR-SSCP) is used to detect tumor suppressor gene P53 mutations in exfoliated cells in stool, but it lacks specificity

[0007] 2. Serological examination of colorectal cancer: mainly radioimmunoassay detection of carcinoembryonic antigen (CEA) in serum, detection of monoclonal antibodies derived from colorectal cancer such as CEA, CA-50, CA195, CA199, etc. It has poor specificity and is not suitable for large-scale censuses

[0008] 3. Biological examination: flow cytometry is mainly used to detect the number of aneuploid cells in colorectal cancer tissue to diagnose colorectal cancer, and its fatal shortcoming is still poor diagnostic specificity

[0009] 4. Use immunohistochemical method, multi-step enzyme chain reaction (PCR), PCR-SSCP, nucleic acid probe hybridization method to detect the protein expression and gene mutation of ras, p53, p16, nm23 and other genes to diagnose colorectal cancer, but for The diagnosis of early colorectal cancer and the diagnostic specificity and sensitivity of colorectal cancer liver metastases need to be further improved

[0010] 5. Although endoscopic examination, ultrasonographic examination and imaging examination can detect early colorectal cancer, they are not suitable for large-scale general screening because asymptomatic carcinoma in situ patients are not easy to accept such examinations

[0011] So far, all relevant studies have taken colorectal cancer patients as samples, and it remains to be further verified how effective these detection indicators are in the screening of colorectal high-risk groups or the general population

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