The invention uses serum specificity protein group fingerprint diagram to diagnose carcinoma of colon and rectal carcinoma in early stage, the invention produces the protein group template at first, selects the outer circle blood of confirmed sufferers, benign sufferer and normal people. Separates the serum in 4 degrees centigrade cold-storage, absorbs the serum centrifugally, packages, backup in subzero 80 degrees centigrade; when in detection, it is diluted according to a density; uses the metal chip of America saifuji corporation; sets in the fly mass spectrum device of America safifuji corporation, analyzes the protein group wave spectrum of the sample serum; compares and analyzes the serum protein group wave spectrum of the carcinoma of colon and rectal carcinoma sufferers, the benign sufferer and health people, acquires the special protein peak in the serum; forms the protein template by combining protein peak in serum, uses the template double-blind method to carry on clinic sifting experiment, confirms the positive predicting valve, negative predicting valve, sensitivity and specificity of the fingerprint diagram.