502results about How to "Efficient detection method" patented technology

The invention relates to a non-labeled electrochemical immune sensor for detecting diethylstilbestrol and a preparation method and application of the sensor. The immune sensor comprises a substrate electrode; and after nanometer gold is deposited on the surface of the substrate electrode, a graphene-diethylstilbestrol-chitosan complex is modified, and non-specific active loci are sealed by bovineserum albumin. The preparation method comprises the following step of: after depositing the nanometer gold on the surface of the substrate electrode, enveloping graphene and the diethylstilbestrol byusing chitosan and fixing to the surface of the electrode. In the immune sensor, on the basis of a competitive mode of immune reaction, the immune reaction is monitored by taking K3Fe(CN)6 as a probeand by differential pulse voltammetry, so the immune sensor can be used for detecting the diethylstilbestrol. The non-labeled electrochemical immune sensor has the characteristics of high sensitivityand specificity, quick on-site detection, low cost and the like; and a detection method is simple and wide in application range, the detection limit can reach 0.1 ng / ml, and the linear range is between 0.5 and 1,500 ng / ml.

The invention discloses a satellite communication network abnormity detection method based on network control. A fuzzy C-means clustering algorithm is utilized to study normal audit data and an abnormity detection mechanism is established, so that a post analysis on an abnormal event is realized; a support vector data description single classifier is utilized to summarize a normal short sequence and an abnormal short sequence as well as a hidden Markov model is used to carry out abnormity detection on communication signalings of all earth stations in a whole time slot, so that real-time detection based on the communication signalings can be realized; and by utilizing the above-mentioned methods of the post analysis and the real-time detection, a satellite communication network abnormity detection prototype system based on network control is realized. According to the invention, the post analysis method is suitable for large-scale data as well as a declaration omission rate and a misdeclaration rate are low; and the real-time detection method has a good detection performance as well as signaling detection time is short and the reaction speed is fast; therefore, an on-line detection object can be realized and safety of a signaling system can be enhanced.

The invention discloses a moving object detection method based on a Gaussian mixture model and superpixel segmentation. The task of moving object detection is extracting objects of interest as many as possible from a complex scene and presenting the results in the form of binary images. The method is characterized by, to begin with, carrying out background modeling by utilizing the Gaussian mixture model and obtaining a background image of the current frame; then, carrying out superpixel segmentation on the current frame through an SLIC(simple linear iterative clustering) algorithm; and finally, carrying out LTP (local ternary pattern) texture feature extraction on the segmented images and background images, and then, carrying out comparison to obtain a moving object. The beneficial effects of the method are that the method can detect the moving object in real time according to the video images captured by cameras; and the detection method is efficient and accurate.

The invention discloses a simple instant operating condition tail gas detecting device for gasoline motor car and its detecting method. The device includes a primary computer, driver assistant device, printer, bottom plate power measuring device and tail gas quality analyzing system, it uses ASM series of bottom plate power measuring device of German MAHA corporation and VMAS car tail gas quality analyzing system of American Sensors corporation, the system includes gas analyzing device, flux gauge and motor tachometer. The primary computer stores the operation series of the tail gas detection, which orders the driver assistant device to execute the tail gas detecting step in instant operating condition, and outputs the detected result to the printer. The method is more scientific, and it is very effective.

The invention belongs to the technical field of intelligent traffic management, and particularly relates to a method for detecting motor vehicle green light follow-up traffic violation based on video. The method comprises the following steps that when a green light is on and the road ahead is jammed seriously, warning information is displayed on a no passing warning board to prompt vehicles which go straight not to enter an intersection; vehicles which still go straight continuously when the warning board is lighted are ranked as the vehicles suspected in violation of regulation, and are traced; the vehicles suspected in violation of regulation are traced continuously, and a complete track of the vehicles driving at the intersection is obtained; and when a red light is on, if the vehicles suspected in violation of regulation are still at a central no parking area, the vehicles are in violation of regulation, and violation evidence photos and video are analyzed and obtained. The method is high in precision, plays a role of warning, and supplies a powerful solution to solve urban traffic jam problem, especially green light follow-up traffic violations.

The invention belongs to the technical field of marine organism pathogen detection, and in particular relates to a multiple-PCR detection method and a kit for simultaneously detecting WSSV (white spot syndrome virus), AHPND (acute hepatopancreatic necrosis disease), EHP (enterocytozoon hepatopenaei) and IHHNV (infectious hypodermal and hepatopancreatic necrosis virus) in prawns. With the application of the multiple-PCR detection method, amplification of four pathogen DNAs can be simultaneously conducted in one time by virtue of special detection primers, namely WSSV F and WSSV R, AHPND F and AHPND R, EHP F and EHP R as well as IHHNV F and IHHNV R of the WSSV, the AHPND, the EHP and the IHHNV. The multiple-PCR detection method provided by the invention is convenient and rapid, low in detection limit, strong in specificity, high in sensitivity and high in accuracy rate and is capable of simultaneously detecting the white spot syndrome virus, the acute hepatopancreatic necrosis disease, the enterocytozoon hepatopenaei and the infectious hypodermal and hepatopancreatic necrosis virus in the prawns.

The invention discloses a method for detecting a sulfanilamide medicine and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a sulfanilamide medicine monoclonal antibody and the sulfanilamide medicine. The sulfanilamide medicine monoclonal antibody is secreted by the hybridoma cell line SAs of a sulfanilamide monoclonal antibody, whose preservation number is CGMCC No. 3393. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the sulfanilamide medicine in products (especially milk, pork, chicken, eggs, honey, fish, shrimp and the like) eaten by animals or people. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the sulfanilamide medicine monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.

The invention provides an elisa kit for detecting cephalo-type medicine, containing: an ELIAS plate peridium of which is provided with peridium source, an enzyme label, cephalo-type medicine specific antibody working solution (contained when the peridium on the ELIAS plate is antigen and the enzyme label is enzyme label anti antibody or when the peridium on the ELIAS plate is anti antibody and theenzyme label is enzyme label antigen), ceftiofur standard solution, substrate developer, stop solution, concentrated cleaning solution and concentrated complex solution. The invention also disclosesa method applying the elisa kit for detecting cephalo-type medicine, including: firstly sample preprocessing is carried out, then the elisa kit is used for detection, and finally the detection result is analyzed. The elisa kit provided by the invention can be applied to detecting residual quantity of cephalo-type medicine in samples such as animal tissue (chicken, pork, fish and shrimp) and milk, etc, the operation is easy, the cost is low, the sensitivity is high, and the elisa kit is applied to on-site supervision and numerous sample screening.

A panel defect detection method and an organic light emitting display device. A region having a high probability of a panel defect is intensively sensed through panel defect detection based on sensing results of characteristic values according to subpixels on sensing subpixel lines in an amount equal to the number of sensing subpixel lines preset in specific regions rather than all regions of a display panel. Rates and accuracy in detection of panel defects can be improved.

The invention discloses a method and a device for accurately detecting and correcting parallelism of an ion beam, relates to an ion implantation machine, and belongs to the field of semiconductor integrated circuit device manufacturing. The device for detecting and correcting the parallelism comprises a parallelism correcting magnet, a correcting magnet power supply, a moving Faraday cup, a sampling Faraday cup, a dosage detector, a digital scanning generator, a motor motion controller, and a control computer. The parallelism correcting magnet is arranged at a proper position behind an electrostatic scanning plate, and a magnet exciting coil of the parallelism correcting magnet is connected with the correcting magnet power supply. The output of the sampling Faraday cup is connected with the dosage detector; the output of the digital scanning generator is connected with a scanning amplifier and the scanning amplifier is connected with the electrostatic scanning plate. The output of the motor motion controller is connected with a drive motor of the moving Faraday cup. The moving Faraday cup is driven by the drive motor and is capable of moving along the X horizontal direction in a target chamber. The correcting magnet power supply, the moving Faraday cup, the sampling Faraday cup, the dosage detector, the digital scanning generator, the motor motion controller are connected with the control computer, and are coordinated and controlled by the computer. The method for detecting and correcting the parallelism of the ion beam comprises the following steps of data detection, data processing, parameter adjustment, and the like. Error data of the parallelism of the ion beam is detected by moving the Faraday cup back and forth, and the parallelism of the scanning ion beam reaches an error range by adjusting the setting current of the magnet power supply repeatedly. The method and the device can automatically realize accurate detection and correction of the parallelism.

The invention discloses a quick detecting method of the edible vegetable oil quality through using an attenuated total reflection Fourier infrared spectroscopy, and the problems that the current detecting method can not verify whether the edible vegetable oil is repeatedly heated at high temperature or whether swill-cooked dirty oil is mixed in fresh edible vegetable oil can be solved. The method comprises the steps of: indicating the change of oil ingredients by fully utilizing the changes of the peak shape and the peak height of an infrared second derivative spectrometry; measuring the content of trans-fatty acids in the edible vegetable oil through absorbance of a characteristic absorption peak at 966.50cm<-1>; judging whether the edible vegetable oil is heated through the change of the characteristic peak at 988cm<-1>, and calculating the heating time; and determining whether the edible vegetable oil is swill-cooked dirty oil through the change of the characteristic absorption peak at 988cm<-1>. The quick detecting method of the edible vegetable oil quality is simple in operation, low in cost, free of pollution, high in detecting precision, and quick and accurate and can be capable of judging an edible vegetable oil sample to be detected which is repeatedly heated at the high temperature, the swill-cooked dirty oil and the swill-cooked dirty oil mixed in the fresh edible vegetable oil.

The invention provides an enzyme-linked immunosorbent kit for inspecting sulfa drugs, comprising an ELISA plate which is coated with coating antigen, an enzyme label, sulfa drug specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), sulfamethoxy-isoxazole standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the sulfa drugs, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the residual amount of the sulfa drugs in animal tissues(chicken, pork, fish and shrimp), honey, eggs, milk, feeds and other samples, the operation is simple, the cost is low, the sensitivity is high and the enzyme-linked immunosorbent kit can be monitore d on-site and is applicable in screening mass samples.

The invention relates to an automatic parking space tracking method. The automatic parking space tracking method comprises the steps of obtaining parking space information, wherein the parking space information comprises absolute coordinates and directions of a parking space; obtaining vehicle position information, wherein the vehicle position information comprises the absolute coordinates and heading angles of the vehicle; establishing a relative coordinate system between the parking space and the vehicle by taking the absolute coordinate of the vehicle as an original point, and acquiring therelative coordinate of the parking space; and planning a parking path according to the relative coordinates of the parking space, detecting whether obstacles exist or not in the automatic parking process, if yes, stopping parking of the vehicle and reversely driving for a preset distance, otherwise, finishing the automatic parking process according to the parking path. According to the automaticparking space tracking method, the obstacles can be detected through the change of the acceleration of the vehicle in the parking process, various parameters of the obstacles are further obtained through a 360-degree panoramic system, and different responses are made according to different properties of different obstacles, so that the intelligence of the automatic parking process is improved, andthe trouble caused by frequent replacement of the parking paths is avoided.

The present invention relates to the field of the laser cutting, especially to a vision auxiliary sheet material excess material stock layout method. The method comprises the following steps: (1) performing camera calibration; (2) collecting an image; (3) extracting the contour of an area having been cut; (4) selecting a cutting figure and setting parameters; (5) dragging a figure and mapping the figure to a visualization window; (6) determining a stock layout position; (7) finishing the stock layout; (8) determining whether there are excess materials for usage; and (9) finishing the stock layout of the sheet materials, and generating the processing position instruction of the cutting figure. Compared with the prior art, the vision auxiliary sheet material excess material stock layout method can realize automatic detection and accurate determination and enhance the management and the application of the sheet material excess material so as to greatly reduce the excess material waste and the material cost of the manufacturing enterprise, allow operators to easily move the parts to be processed and easily determine whether the area of the excess material can cut parts to be processed or not and provide an effective detection and determination method for full utilization of the excess materials.

The invention belongs to the technical fields of application methodology and electronic tongue detection, and discloses a difference degree calculation method and tea quality identification method based on the electronic tongue detection. The difference degree calculation method comprises the steps of electronic tongue detection, PCA analysis, and difference degree calculation; wherein the difference degree calculation adopts a recently improved calculation mode, each coordinate in the PCA score matrix is taken into account during the calculation process in a form of distance between each coordinate and the center point so as to reduce the affect of the random errors during the experiment process, and an sample acceptable fluctuation range is formed at the same time. The tea quality identification is on the basis of the electronic tongue difference degree detection of tea samples, the same tea species is taken as the standard, the credibility interval is set according to the difference degree in each group, and thus established is a standard sample experiment measured value error range. If the measured value of a tea sample is in the credibility interval, the sample is qualified; and if the measured value of a tea sample is not in the credibility interval, the sample is disqualified. The method can be used to control tea quality, and is a more efficient and stable detection method for market, enterprises, and detection organizations.

The invention discloses a detection method of melamine, belonging to the fast detection method for the adulteration components of food and feeds. The technical scheme of the detection method comprises the steps of: (1) processing impurity removal pretreatment on an object sample to obtain filtrate; (2) adding 5-10% acetic acid, 1-5% cyanuric acid, 5-10% oxalic acid or 5-10% tannic acid into the filtrate, visually observing the generation of turbidity, and if there is turbidity generation, considering the melamine content of the sample higher than 2mg / kg. The detection method is used for the melamine of milk products and feeds, which is characterized in specificity, high efficiency, high speed, accuracy and stability. The detection method has simple operation and low detection cost, thus is beneficial for actual popularization and application, to satisfy the actual demands of liquid milk, powdered milk and feed enterprises and the like and provide a fast and effective detection method for the melamine of food and feed under government supervision.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting carbofuran. The ELISA kit comprises an ELISA plate coated with a carbofuran conjugated antigen, a carbofuran monoclonal antibody, an enzyme labeled antibody, a carbofuran standard solution, a substrate color development solution, a stop buffer, a cleaning solution and a re-dissolution solution. The invention further discloses a method for applying the ELISA kit to detection of the carbofuran. The method comprises the following steps: a sample is pretreated firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of the carbofuran in vegetables and tea leaves, the operation is simple and convenient, the cost is low, the sensitivity is high, on-site supervision can be realized, and the kit is suitable for screening of a large quantity of samples.

The invention discloses an evolutionary computation-based method for diagnosing compressor fault. The method comprises the following steps: data about the working condition of a compressor is collected and acquired; an observation point is taken as a node, and the correlation between observation points produced by the actual compressor physics; a weighting undirected graph G(V,A) is set up for measured data; the distance between fault data points is gauged by means of a relational graph instead of the traditional Euclidean distance method; the data about the fault of the compressor is converted into a problem with the partitioning of the weighting undirected graph G; the Pareto Principle structural map is adopted for partitioning cost function; an evolutionary calculation chart partitioning computation based on the improvement on the complexity system theory is designed to partitioning the relational graph; and the clustering / classification of the compressor fault data is realized through the optimal computation of the cost function, so that the purpose of fault diagnosis can be achieved.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention relates to the field of aquatic product detection, and discloses a method for detecting the heavy metal content of an aquatic product. The method comprises the following steps: 1), pretreating: removing inedible inner organs, fish skin, fish scales, fish bones and other hard tissues of the aquatic product, then crushing remaining tissues into minced meat, adding an aqueous Triton X-100 solution and a magnetic heavy metal adsorbent, mixing and stirring; 2), performing heavy metal extraction; 3), calibrating a standard curve; 4), performing heavy metal content detection. By the heavy metal detection method, strong acid and a microwave digestion instrument are not required to be used for digestion, operation is simple, safety and environment friendliness are achieved, the effectof enriching heavy metals in the aquatic product is good, the magnetic heavy metal adsorbent can be reused, remaining impurities of the aquatic product have no influence on a detection sample duringthe detection, and a detection result is accurate, so that the heavy metal detection method is a very accurate, efficient and environment-friendly detection method and has a practical significance inimproving the food safety.

The invention discloses a lithium battery rapid charging management system and method for an electric power tunnel inspection robot. The lithium battery rapid charging management system comprises an upper computer, an intelligent robot charging dispatching module, a battery management module, a track charging contact mechanism and a fixed point intelligent rapid charging apparatus, wherein the upper computer communicates with the intelligent robot charging dispatching module and the fixed point intelligent rapid charging apparatus separately; the intelligent robot charging dispatching module communicates with the battery management module; and the battery management module establishes communication with the fixed point intelligent rapid charging apparatus through the track charging contact mechanism. The lithium battery rapid charging management system and method have the beneficial effects that the charging equipment is mounted on the existing track, so that low cost is realized; the charging management system can realize real-time communication with the robot and the upper computer; the positions of each charging equipment in the tunnel are stored in the robot, so that the most reasonable charging equipment can be found according to positions and respective electric quantity; and therefore, the stable electric power supply and effective operation of the robot are ensured.

The invention discloses a hapten, and a preparation method and application thereof, particularly a methyl parathion hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the methyl parathion hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of methyl parathion residues in corn and apple samples and screening of mass samples.

The invention discloses a direct current micro voltage / micro current detection device and a detection method thereof. The detection device comprises an oscillation circuit, a digital signal amplifying circuit, a subtractor and a numerical value scaling unit, wherein the oscillation circuit comprises a first voltage-controlled oscillator and a second voltage-controlled oscillator, the parameters of the first voltage-controlled oscillator and the second voltage-controlled oscillator are the same, the input of the first voltage-controlled oscillator is connected with the output end of a signal to be detected or is grounded, the input of the second voltage-controlled oscillator is grounded or is connected with the output end of the signal to be detected, the output of the first voltage-controlled oscillator and the output of the second voltage-controlled oscillator are respectively connected with the input of the digital signal amplifying circuit; the output of the digital signal amplifying circuit is connected with the input of the subtractor, and the output of the subtractor is connected with the input of the numerical value scaling unit. The method comprises the following steps: the detection device respectively converts an input signal into oscillation frequencies by the first voltage-controlled oscillator and the second voltage-controlled oscillator, and amplifying, subtracting and conversion are carried out, so as to judge whether a direct current micro voltage or direct current micro current is input or not.

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

The invention relates to a mitochondria SNP (single nucleotide polymorphism) fluorescence-labeling multiple amplification kit capable of detecting 61 SNP loci at the same time. According to a mitochondria phylogenetic tree, based on genetic characteristics of Chinese populatio, 61 SNP loci which are high in polymorphism, low in mutation rate and strong in parting capacity are selected, wherein the 61 SNP lotuses comprise 54 loci in a coding region, 7 loci in a control region; the kit can be used for detecting the 61 SNP loci by virtue of specific primers. The kit which is used as a mitochondria detecting system is capable of carrying out amplification in three tubes at the same time and performing electrophoresis at the same time, so that haplotype diversity reaches 98.6%, and therefore, the mitochondria SNP fluorescence-labeling multiple amplification kit can be used as a kit for identifying the same maternal line, after autosome STR and Y chromosome STR.

The invention provides a baicalin content detecting method and system based on terahertz time domain spectroscopy. The method comprises the steps of: preparing samples of mixtures with different baicalin contents; collecting terahertz time domain signals of no-load and the samples; performing Fourier transform on the collected terahertz time domain signals of no-load and the samples to obtain terahertz frequency domain signals of reference and the samples, and performing calculation to obtain terahertz absorption spectra of the mixtures; p reprocessing the absorption data of the spectra, calculating the similarity of the absorption spectrum of the mixture of each ratio, removing the abnormal spectra, and dividing the samples into a correction set and a verification set; using the terahertzabsorption spectra of the calibration set and the corresponding baicalin concentration information as input, establishing a baicalin content prediction model based on sample data in the calibration set sample data by using partial least squares method, verifying the prediction model, and outputting the prediction model that meets the requirements; and detecting a baicalin sample to be detected byusing the prediction model, and outputting a detection result.

The invention discloses a detection method of imperfect rice grains, and belongs to the field of non-destructive detection during storage and processing of agricultural products. The detection methodof the imperfect rice grains is achieved by acquiring images by a computer image acquisition system to obtain images of perfect grains and the imperfect grains, processing the images to obtain relevant parameters of the images, selecting appropriate parameters, establishing a model, and distinguishing the imperfect grains. Compared with other detection ways, the detection method provided by the invention has the characteristics of fastness, convenience and high efficiency and is of a great significance in grain safety quality.

The invention discloses a cutter breakage detection method based on internal data of a numerical control system, comprising the following steps: (1) collecting the internal data of a numerical control system as cutter data, recording manufacturing resource data, and intercepting machining state data from the cutter data and pre-filtering the cutter data to get low-frequency data of cutter machining state; (2) extracting signal features characterizing cutter state according to the low-frequency data of cutter machining state to form a feature vector fea={fea<1>, fea<2>,...,fea<m>}, wherein i is the sample number of cutter data, and m is the number of feature dimensions; and (3) carrying out model training according to the feature vector and a corresponding category thereof to get a training model, judging the state of a cutter according to the training model, and completing cutter breakage detection in this way. A real-time accurate cutter breakage detection function is achieved. Subsequent scraping of cutters and parts is avoided. The first pass yield of products of an automatic production line is improved.

It is a measurement for content of fly ash in the new-mixed concrete and especially relates to the measurement for concrete content. The method in this invention is the following: first to separate through partition method carpolite, sand and mixture of cement and fly ash in the specimen of new-mixed concrete and to weigh them after cooling and drying; then to measure the cement, fly ash density in the material used in the concrete or sand plasm and density of the recycled mucus mixture; then to measure the fly ash content through simple calculation. This invention improves the measurement for the concrete content and can be widely used in the measurement of the solid waste such as fly ash in the new-mixed concrete.

The invention provides an enzyme-linked immunosorbent kit for inspecting porcine immunoglobulin G, comprising an ELISA plate which is coated with coating antigen, an enzyme label, porcine immunoglobulin G specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), porcine immunoglobulin G standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the porcine immunoglobulin G, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the content ofthe porcine immunoglobulin G in porcine plasma protein powder and other samples, the operation is simple, the cost is low, and the enzyme-linked immunosorbent kit can be monitored on-site and is appl icable in screening mass samples.