12results about How to "Efficient detection method" patented technology

The invention provides an electronic one-tube automatic detection system and a detection method for streptococcus agalactiae or gonococci, through an LAMP and CRISPR-Cas12a combined mediated one-tube visual streptococcus agalactiae or gonococci detection system, in combination with an electronic automation technology, an LAMP reaction mixture and a CRISPR-Cas12a reaction mixture are mixed to form a one-tube reaction system, and the one-tube reaction system is used for detecting the streptococcus agalactiae or gonococci. Automatic mixing, preheating reaction and fluorescence detection of various detection reagents and samples are realized, and visual display is performed on detection results by adopting visual equipment. Visual detection of bacteria is achieved by detecting fluorescence signals, rapid positive or quantity detection results can be provided for patients and other users, and clinical detection efficiency is improved.

The invention discloses an improved YOLOv8-based capsicum flower front shape detection method and system, relates to an intelligent agricultural technology, and solves the technical problems that an existing capsicum flower detection method for a point pollination robot cannot realize effective balance between identification efficiency and identification accuracy and affects efficient and accurate pollination of the point pollination robot. The method comprises the steps that capsicum flower data is collected to make a data set, the data set is utilized to train and verify an improved YOLOv8 model, finally, the trained improved YOLOv8 model is utilized to recognize the front shape of capsicum flowers, a backbone network in the improved YOLOv8 model uses C2f-CAA-Star, a high-order screening feature pyramid network is used in a Neck network, and a Detect-E model is adopted in the head of the model. According to the method, the identification capability of the model on different morphological characteristics of the pepper flowers is further improved, and the detection accuracy under a complex background is ensured.

The invention discloses a hapten for detecting the content of isofenphos-methyl and application of the hapten, particularly relates to the hapten for detecting the content of isofenphos-methyl and a preparation method of the hapten, and further discloses application of the hapten in the aspects of antibodies and kits. The enzyme linked immunosorbent assay kit established by aiming at the hapten for detecting the content of isofenphos-methyl can realize rapid detection of a large batch of samples to be detected, is convenient to use and low in detection cost, the detection method is efficient, accurate and rapid, can simultaneously detect a large batch of samples, and is suitable for on-site monitoring of isofenphos-methyl in fruits, vegetables and traditional Chinese medicinal materials and screening of a large number of samples.

The invention belongs to the technical field of pharmaceutical analysis, particularly relates to a method for detecting methylamine in Vonoprasone fumarate, and provides a convenient, efficient and accurate detection method for solving the problem of detection of methylamine in Vonoprasone fumarate. Derivative products are detected by liquid chromatography, the content of methylamine in the Vonoprazan fumarate can be detected, so that medication safety is effectively guaranteed, quality control of the Vonoprazan fumarate is facilitated, the method is convenient, efficient and accurate, the system applicability, repeatability, specificity and accuracy completely conform to the guidance principle verified by methods in Chinese pharmacopoeia, and the method is suitable for popularization and application. The method can be used for quality control of the Vonoprasone fumarate.

The invention discloses a method for simultaneously detecting rice yeast acid, isorice yeast acid and toxoflavin, and belongs to the technical field of food detection. The method for simultaneously detecting the rice yeast acid, the isorice yeast acid and the toxoflavin comprises the following steps: mixing a to-be-detected sample and an extracting solution, extracting, and purifying to obtain a to-be-detected sample solution; detecting the to-be-detected sample solution by adopting a liquid chromatography-tandem mass spectrometry method; the extracting solution is a mixed solution of acetonitrile and acetic acid. According to the method provided by the invention, the sample pretreatment process is simplified, the problem of simultaneous extraction of the rice yeast acid, the isorice yeast acid and the toxoflavin with relatively large polarity difference is solved, simultaneous determination through one-time extraction is realized, the test time is shortened, the contents of the rice yeast acid, the isorice yeast acid and the toxoflavin in the fresh wet powder can be simply and efficiently detected and confirmed, and the method is suitable for large-scale popularization and application. The measurement result is accurate and reliable.

The invention discloses a transformer anti-short-circuit capability evaluation system of a power system, relates to a transformer anti-short-circuit capability evaluation device, and aims to solve the problem of low reliability of an existing method for evaluating the anti-short-circuit capability of a transformer. According to the invention, the short-circuit current calculation module calculates the short-circuit current when various external short circuits occur; the first evaluation result output module obtains a transformer anti-short-circuit capability evaluation result A based on the short-circuit current; the short-circuit electrodynamic force output module establishes a finite element analysis model according to the short-circuit current calculated by the short-circuit current calculation module, and obtains corresponding short-circuit electrodynamic force; the second evaluation result output module is used for carrying out anti-short-circuit capability analysis on the power transformer according to the short-circuit electrodynamic force obtained by the short-circuit electrodynamic force output module to obtain an evaluation result B; and the comprehensive evaluation result output module is used for obtaining a final evaluation result by utilizing a comprehensive evaluation analysis method according to the evaluation result A and the evaluation result B. The method has the advantage that the reliability of short-circuit capability evaluation is improved.

The application discloses a method for quality prediction of tinplate cans based on iron ion migration amount, which comprises the following steps: sealing and soaking the tinplate cans filled with acetic acid, and performing temperature acceleration test; sampling on the third day of the temperature acceleration test, and detecting the iron ion migration amount; sampling on the tenth day of the temperature acceleration test, and detecting the iron ion migration amount; and predicting the quality of the tinplate cans based on the iron ion migration amounts on the third day and the tenth day. The application takes the migration amount on the third day as a short-term risk indicator, and takes the migration amount on the tenth day as a key quality determination standard of long-term stability indicator. Based on the detection results of the two time points, the iron ion migration trend of the tinplate cans within a six-month shelf period can be reliably predicted, the sensory quality and stability of beer products can be ensured, clear and quantitative delivery access conditions of suppliers can be set according to the detection results, the risk of beer quality deterioration caused by quality problems of packaging materials can be avoided from the source, early prediction and precise control of the quality of packaging materials are realized, and the overall quality management level is improved.

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of ammonia in methamphetamine, which is a convenient, efficient and accurate detection method for solving the problem of ammonia detection in methamphetamine. The method uses gas chromatography to detect the content of ammonia in methamphetamine, has low detection cost, and is convenient for quality control of methamphetamine. The method is convenient, efficient and accurate, and fully meets the guiding principles of method verification of Chinese Pharmacopoeia in terms of system applicability, repeatability, specificity and accuracy, and can be used for quality control of methamphetamine.

The invention relates to the technical field of fluorescent nano sensors, in particular to a synthesis method of a fluorescent nano sensor for monitoring the content of ferric ions in sewage. The method comprises the following steps: 1, carrying out anhydrous and anaerobic treatment on a reactor, cooling to room temperature, adding an F127 or P123 solution into the reactor, and adding 10-ethyl-3, 7-diformaldehyde phenothiazine under a stirring condition to synthesize fluorescein isothiocyanate with the concentration of (1-9) * 10 <-4 > mol / L; and 2, adding ethanol into fluorescein isothiocyanate, fully stirring, adding tetraethoxysilane, reacting, adding a terminator, continuously stirring, filtering by using a water-based filter membrane, and dialyzing by using deionized water until particles are neutral, thereby obtaining the fluorescent nano sensor. The size of the synthesized fluorescent nano sensor is lower than 20 nm, the water compatibility is good, the luminous intensity in water is high, the online detection capability is achieved, the detection method is visual and effective, and the method can be suitable for chemical sewage and domestic sewage.

The invention belongs to the technical field of pharmaceutical analysis, particularly relates to a method for detecting 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole, and provides a convenient, efficient and accurate detection method for solving the problem of detection of 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole. The content of 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole can be detected, so that the medication safety is effectively guaranteed, the quality control of voriconazole is facilitated, the method is convenient, efficient and accurate, the system applicability, repeatability, specificity and accuracy completely conform to the guidance principle verified by methods in Chinese Pharmacopoeia, and the method is suitable for popularization and application. The method can be used for quality control of voriconazole.

This invention provides a method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. The method involves derivatizing the sample with sodium iodide-ascorbic acid and detecting the sample using a headspace gas chromatograph equipped with an ECD detector. The chromatographic column is an Agilent DB-624 30m × 0.53mm × 3μm column, the injection port temperature is 220℃, the detector temperature is 250℃, and the carrier gas is N2. 2， Flow rate: 1.5 mL / min, temperature programmed: initial column temperature 70℃, increased to 150℃ at a rate of 5℃ / min and held for 0 min; then run at 240℃ and held for 5 min, flow rate 3 mL / min. This invention provides a convenient, efficient, and accurate method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. This method uses gas chromatography to detect the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate, thereby effectively ensuring medication safety.

The application belongs to the technical field of large-scale flat plate reflection array fault detection, and particularly relates to a detection device and method for a reconfigurable reflection array unit, the device comprising an isolation chip, a driving chip, a main control chip and a test circuit, the isolation chip is used for receiving test instructions issued by a computer and converting the test instructions into driving signals; the driving chip is connected with the isolation chip and is used for converting the driving signals into level signals; the main control chip is connected with the driving chip and is used for generating pin control signals; the test circuit is connected with the main control chip and is used for collecting pin current values in the reconfigurable reflection array unit; the device further comprises a power supply circuit, the power supply circuit supplies power to the isolation chip, the driving chip and the main control chip respectively, and the application can realize detection on the working condition of a large-scale reconfigurable reflection array unit, and has a simple structure and higher efficiency.