Enzyme-linked immunological kit for detecting neomycin drug and method

Abstract

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

Description

technical field

[0001] The invention relates to the field of immunological detection, in particular to an enzyme-linked immunoimmunoassay kit for detecting neomycin drugs in animal-derived foods such as chicken, chicken liver, pork, pig liver, milk powder, eggs and feed, and its Detection method. Background technique

[0002] Neomycin has a broad antibacterial spectrum and has good inhibitory effects on Gram-positive bacteria and Mycobacterium tuberculosis. As a common veterinary drug, it is widely used in animal husbandry production. In veterinary medicine, neomycin is mainly used to treat bacterial enteritis in livestock and poultry. In livestock and poultry breeding, it can improve feed utilization and promote animal growth, but because of its high toxicity, residues in animal foods can cause greater potential harm to consumers and pose a threat to food hygiene and public health .

[0003] The chemical methods for detecting neomycin residue mainly include thin-layer c...

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