37 results about "Sulfa drug" patented technology

The invention provides an enzyme-linked immunosorbent kit for inspecting sulfa drugs, comprising an ELISA plate which is coated with coating antigen, an enzyme label, sulfa drug specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), sulfamethoxy-isoxazole standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the sulfa drugs, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the residual amount of the sulfa drugs in animal tissues(chicken, pork, fish and shrimp), honey, eggs, milk, feeds and other samples, the operation is simple, the cost is low, the sensitivity is high and the enzyme-linked immunosorbent kit can be monitore d on-site and is applicable in screening mass samples.

The invention relates to a preparation method of a traditional Chinese medicine lotion for curing acute conjunctivitis, which pertains to the technical field of traditional Chinese medicine preparation methods. At present, acute conjunctivitis is cured by adopting a sulfa drug which is easy to cause gastrointestinal tract reaction or crystalluria, hematuria or tetter, antipyretic, leukocyte reduction, etc. The technical proposal of the invention is that: chrysanthemum, mint, cicada slough, mulberry leaf, fructus viticis, taraxacum mongolicum, viola yedoensis makino, herba patriniae, blackberrylily, ampelopsis japonica mak, cyrtomium fortunei, smilax glabra, bistortae, polygonum bistatum, herba lobeliae radicantis, potentilla discolor bunge, barberry root, cayratia japonica, tupan root, prunella vulgaris, clearson, seed of feather cockscomb, pale butter-fly bush bud, pipewort, equisetum hyemale, bat dung, princesplume ladysthumb fruit, and unripe licorice are taken as raw materials, the raw materials are soaked by using 1600ml water for 30mintues and boiled by small fire, residues are filtered by using gauze, thus preparing 1000ml of traditional Chinese medicine liquid which is the traditional Chinese medicine lotion for curing acute conjunctivitis. The lotion of the invention has the advantages of simple preparation method, low toxicity, fewer side effects of the prepared traditional Chinese medicine lotion and being able to directly achieve to the focus.

The invention relates to a sulfa drugs direct competition enzyme-linked immunity detection reagent box, which belongs to the enzyme-linked immunity and the veterinary drug residues detection technical fields. The invention comprises the preparation of an artificial immune antigen which can produce a sulfa drugs antibody, an enzyme label antigen, a sulfa drugs antibody, a standard liquor reagent, a bottom content liquor reagent, a display color liquor reagent, a termination liquor reagent, an enzyme label antigen diluent reagent, a packet sulfa drugs antibody porous polystyrene micro reaction board and a concentration washing liquid reagent. The invention mainly adopts the direct competition enzyme-linked immunity method to detect qualitatively or quantitatively the sulfa drugs residues quantity in the food and feed; the detection limit is larger than 2 Mug / kg, the detection time only need 2 hours; the average recovery is 70 percent to 120 percent, the errors in a batch are smaller than 10 percent, the errors between the batches are smaller than 20 percent, and the invention is characterized by simpleness, rapidness and accurateness, which can be directly used for the detection of the sulfa drugs residues quantity in the food and feed.

The invention belongs to the technical field of chemical detection, and particularly relates to a preparation method of a hydrophilic sulfa drug molecularly imprinted solid-phase extraction column. The preparation method of the hydrophilic sulfa drug molecularly imprinted solid-phase extraction column comprises the following steps: (1) synthesizing a hydrophilic sulfa molecularly imprinted polymer by a bulk polymerization method; (2) characterizing the imprinted polymer obtained in the step (1), and determining the presence of an imprinted binding site; and (3) filling in the polypropylene solid-phase extraction column. The prepared hydrophilic solid-phase extraction column has powerful adsorption function, relatively strong specificity, high recovery rate and relatively low preparation cost, besides, improves the disadvantage of large required solvent amount in a traditional sample pretreatment technology, can be directly used for detection of ten sulfa veterinary drugs in animal-derived foods, and has the characteristics of high selective adsorption and high-efficiency enrichment on the sulfa drugs; and moreover, the solid-phase extraction column can be used for detection of sulfa veterinary drugs in a completely aqueous phase.

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

A gold-linked chromatography test paper box uses for analysis of the sulfonamides excessive remains. The test paper box makes up by plastic shell and test paper core, and the faceplate has sample adding hole and result displaying window. The reaction film is pyroxylin film with recessive test line and comparison line. Gold-linked combine pillow is fiberglass cotton with 2-5 strips mixed gold-linked antibody and the test line of the reaction film is 2-5 strips opposite sulfonamides-carrier albumen unite antigen. Or Gold-linked combine pillow is fiberglass cotton with 2-5 strips gold-linked sulfonamides-carrier albumen unite antigen, while the test line of the reaction film is 2-5 strips corresponding antibody to some sulfonamides. The comparison line of the reaction film is goat antirat or rabbit antirat IgG, or goat anti rabbit IgG, or rabbit-anti carrier albumen IgG. The box can test manifold sulfonamides at the same time and greatly save the test time with strong character, high sensitive, easy, intuitive, nicety and wide applied range, low cost for extending.

Selective enrichment media and methods for selectively growing and detecting Salmonella spp. The media comprise a carbon and nitrogen source, an inorganic salt, a fermentable sugar, one or more selective agents, and an efflux pump inhibitor. Various selective agents include sulfa drugs, surfactants, aminocoumarins, cycloheximide, supravital stains, ascorbic acid, bromobenzoic acid, myricetin, rifamycins, polyketides, and oxazolidinones. Various efflux pump inhibitors include arylpiperazines, such as 1-(1-naphthylmethyl)piperazine, and quinoline derivatives, such as 4-chloroquinoline. The selective agents and efflux pump inhibitors are provided in the media in combinations and amounts that inhibit growth of non-Salmonella microorganisms without substantially affecting growth and metabolism of Salmonella species. Methods of selectively growing and detecting Salmonella species are provided.

A pharmaceutical composition for application to an area of skin of a subject for local and / or systemic treatment of a COX-2 mediated disorder comprises a backing sheet that is flexibly conformable to the area of skin, the backing sheet having opposing surfaces that are respectively distal and proximal to the skin when applied; and a coating on the proximal surface of the backing sheet that comprises (a) an adhesive, (b) an active agent comprising a selective COX-2 inhibitory sulfonamide drug of low water solubility, and (c) a solvent system for the active agent, wherein the active agent is in a therapeutically effective total amount and the solvent system is selected with regard to composition and amount thereof to be effective to maintain the active agent substantially completely in solubilized form. A method of local treatment of a site of pain and / or inflammation in a subject comprises applying the composition to a skin surface of the subject, preferably at a locus overlying or adjacent to the site of pain and / or inflammation, and leaving the composition in place for a time period effective to permit delivery of a locally therapeutic amount of the active agent. A method of systemic treatment of a subject having a COX-2 mediated disorder comprises applying the composition to a skin surface of the subject, and leaving the composition in place for a time period effective to permit transdermal delivery of a therapeutic amount of the active agent.

The invention provides compound sulfa-nitazoxanide soluble powder for pigs and poultry. The soluble powder is prepared from the following components: 20-30g of sulfa-drugs, 4-10.0g of TMP sodium, 1.5-5g of nitazoxanide, 5-25g of a co-solvent and 1-7.5g of a stabilizing agent, and the total amount after the components and auxiliaries are mixed is 100g. The soluble powder can be used for overcoming the defect that sulfa-drugs are invalid to anaerobic bacteria during infection of coccidium mixed with anaerobic bacteria, has excellent effects in preventing and treating protozoon infection and mixed infection of protozoon and bacteria of pigs and poultry, and is valid to drug-resistance coccidium infection which is poor or invalid to sulfa-drugs; nitazoxanide has the defects of water insolubility and alkali solubility, while the co-solvent can be used for improving the water solubility of nitazoxanide, and the stability of nitazoxanide can be improved by adding the stabilizing agent. The compound sulfa-nitazoxanide soluble powder is administrated by drinking, is convenient to use, and has excellent effects on preventing and treating protozoon infection and mixed infection of protozoa and bacteria of pigs and poultry.

The present invention belongs to the field of veterinary medicine residue detecting immunochemistry technology, and discloses one kind of colloidal gold test paper strip for fast detection of sulfa drug residue. The test paper strip consists of sample pad, combining pad, nitrocellulose film, water absorbing pad and PVC back lining. It features the combining pad with coated sulfa drug resisting specific monoclonal antibody-colloidal gold marker, the nitrocellulose film with coated N-sulfanilamino-4-amino benzoic acid-carrier protein coupler as the detection line and coated rabbit antimouse IgG as quality control line. The present invention also discloses the preparation of hybridoma BDRXN-2 with preservation number of CCTCC-C200629 capable of secreting specific sulfa drug recognizing monoclonal antibody. The test paper strip of the present invention has the advantages of high sensitivity, high specificity, simple operation, fast detection and low cost.

The invention discloses a kit and a test strip for detecting sulfonamides and a use thereof. The kit and colloidal gold test strip prepared from a protein flop expressed by a preserved bacterial strain Escherichia coli BL21(DE3)-pET28b-flop are used for detecting sulfonamides. The kit and colloidal gold test strip have the advantages of high sensitivity, high accuracy, low cost, operation simpleness and long shelf-life, realize fast detection of a lot of samples, realizes on-site high flux rapid detection and produces a great effect in sulfonamide detection.

The invention discloses a dipstick used for testing sulfonamides and fluoroquinolones and test method thereof. The dipstick consists of a dipstick body and a microporous reagent, the said microporous reagent contains a freeze-dried sulfonamides monoclonal antibody-colloidal gold marker and a freeze-dried fluoroquinolones monoclonal antibody-colloidal gold marker; the said dipstick body consists of a sample absorption pad, a reaction film, an absorbent pad, a protective film and a bottom patch which are connected in sequence, the said reaction film includes two detection lines and a quality control line, the said two detection lines are respectively coated with sulfonamides hapten carrier protein conjugates and fluoroquinolones hapten carrier protein conjugates, the said quality control line is coated with goat-anti-mouse antibody. Using the dipstick introduced in this invention to test sulfonamides and fluoroquinolones is convenient, fast, intuitive, and accurate. The dipstick is convenient to carry, is wide in application range and low in cost and is easy to promote and use.

Disclosed is a method for preparing sulfonamide compound liposome and its formulation, wherein the preparation comprises the steps of, (1) if sulfonamide has free ammonia, subjecting the sulfonamide molecule to modification, if sulfonamide has no free ammonia, proceeding no modification and entering the next step, (2) dissolving the sulfonamide compound and right amount of phosphatide, cholesterin, anti-oxidant and other liposolubility substances into alcohols, ethers or mixture of alcohol and ether, (3) injecting the solution into 25-65 deg. C 0.2%-0.6% diatomic and tervalency metallic ion solution under magnetic stirring, (4) decompressing for removing alcohol and ether, squeezing through microporous filtering film, (5) charging a definite quantity of EDTA-Na into the liposome by dialysis, removing free diatomic and tervalency metallic ions to obtain other preparations.

The invention provides new medical application of a sulfa drug composition and in particular discloses application of the sulfa drug composition in preparation of medicine for treating foot odor. The dosage form of the medicine for treating foot odor is the dosage form suitable for being directly applied or sprayed or smeared on one or more places of socks, feet, insoles and the inner parts of shoes, and the preferred dosage form is powder, a solution, a cream or a gel agent. The medicine for treating foot odor prepared according to the sulfa drug composition can directly act on one or more places of the socks, the feet, the insoles and the inner parts of the shoes, can effectively kill bacteria causing odor and inhibit growth and breeding of the bacteria so as to avoid odor, and can fundamentally and quickly destroy the growth environment of the bacteria causing odor, therefore having a significant effect.

Selective enrichment media and methods for selectively growing and detecting Salmonella spp. and / or Shiga toxin-producing E. coli. The media may comprise a carbon and nitrogen source, an inorganic salt, a fermentable sugar, one or more selective agents, and an efflux pump inhibitor. Various selective agents include sulfa drugs, surfactants, aminocoumarins, cycloheximide, supravital stains, ascorbic acid, bromobenzoic acid, myricetin, nitrofurantoin, rifamycins, polyketides, and oxazolidinones. Various efflux pump inhibitors include arylpiperazines, such as 1-(1-naphthylmethyl)piperazine, and quinoline derivatives, such as 4-chloroquinoline. Methods of selectively growing and detecting Salmonella and / or Shiga toxin-producing E. coli are provided.

The present invention relates to an aqueous ophthalmic composition that contains (A) vaseline and (B) at least one selected from the group consisting of a vinyl-based polymer compound, a saccharide, an amino acid, polyalcohol, a preservative, a sulfa drug, a vitamin, an inorganic salt compound, an ocular muscle modulator component, a vasoconstrictor, a stabilizer, polyoxyethylene polyoxypropylene glycol, and a vegetable oil.

The invention discloses a preparation method and application of sulphonamide molecular-imprinting solid-phase extraction columella. The preparation method comprises the following steps of: (1) mixing a template molecule, a functional monomer and a cross-linking agent; (2) representing molecular-imprinting polymer particles; (3) filling the molecular-imprinting polymer particles into a solid-phase extraction columella, wetting and washing by using methanol and acetone, sealing in vacuum, wherein the sulphonamide molecular-imprinting polymer is obtained by the following steps of: (a) dissolving the template molecule sulfanilamide and the functional monomer in pore-foaming agent acetonitrile to obtain a mixed solution; (b) carrying out ultrasonic treatment onto the mixed solution by an ultrasonic cleaner to obtain a pre-polymerization system; (c) transferring the pre-polymerized system to a quartz reaction test tube, adding cross-linking agent and imitator; (d) placing the pre-polymerization system under ultraviolet light; (e) directly grinding the imprinted polymer to obtain the imprinted polymer particles. The preparation method of the sulphonamide molecular-imprinting solid-phase extraction columella is high in recovery rate, simple to prepare, low in cost and capable of being directly used for selective adsorption and efficient enrichment of sulfadiazine, sulfamerazine and sulfadimidine in animal-origin food.

The present invention provides an aqueous solution containing pranoprofen or a pharmacologically acceptable salt thereof, and a sulfa drug, and a method for making pranoprofen or a pharmacologically acceptable salt thereof stable to light in an aqueous solution, which includes adding a sulfa drug to the aqueous solution containing pranoprofen or a pharmacologically acceptable salt thereof.

A soluble gauze with inflammation-relieving, antibacterial and staltic function is prepared through immersing the absorbent gauze in the solution of monochloroacetic acid, reaction of said gauze on amino acid, immersing in saturated solution of NaOH, etherifying reaction, putting the gauze in the aqueous solution of alcohol, dropping hydrochloric acid to regulate pH value to 5.4-7.6, washing, and reaction between treated gauze and sulfa drug. Its advantages are high effect to provide nutrients to wound and promote healing of wound, and durable action.

The invention discloses a preparation method of an antibacterial NCC (nano-crystalline cellulose) film with sulfonamide medicine groups grafted on the surface. The method comprises the following steps of (1) dispersing NCC with negative electricity on the surface into deionized water to obtain an NCC dispersion liquid; (2) using an NaOH solution for regulating the pH value of the NCC dispersion liquid to be 7.5 to 9.0; (3) adding a sulfonamide medicine solution into the obtained solution; fast raising the temperature to 80 to 95 DEG C; performing ultrasonic agitation reaction; lowering the temperature; performing discharging; (4) preparing the antibacterial NCC film with sulfonamide medicine groups grafted on the surface from the product solution obtained in the step (3) by using a filtering method. Amino groups of sulfonamide medicine are used for being combined with hydroxyls of the NCC; the NCC film with the antibacterial performance is obtained; through the addition of the sulfonamide, the antibacterial performance of the NCC film is improved; the storage conditions are expanded; the application range of the NCC film is expanded; meanwhile, the price of the raw materials is low; the resources are wide; the method is applicable to general production and popularization.

The invention relates to a molecularly imprinted polymer material used for purifying sulfonamide drugs in water. Template molecules, functional monomers and crosslinking agents undergo free radical polymerization under the action of an initiator to form a high degree of crosslinking by emulsion polymerization. microsphere molecular polymer. The present invention proposes a molecularly imprinted polymer material for purifying sulfa drugs in water. By enriching and purifying the target pollutants, the molecularly imprinted polymer material is effective against water pollutants such as sulfamethazine, sulfadiazine, Seven sulfa antibacterial drugs including sulfamethoxazine, sulfisoxazole, sulfathiazole, sulfa-methoxine and sulfamethazine have high adsorption capacity and good selectivity.

The invention belongs to the technical field of chemical detection, and particularly relates to a preparation method of a hydrophilic sulfa drug molecularly imprinted solid-phase extraction column. The preparation method of the hydrophilic sulfa drug molecularly imprinted solid-phase extraction column comprises the following steps: (1) synthesizing a hydrophilic sulfa molecularly imprinted polymer by a bulk polymerization method; (2) characterizing the imprinted polymer obtained in the step (1), and determining the presence of an imprinted binding site; and (3) filling in the polypropylene solid-phase extraction column. The prepared hydrophilic solid-phase extraction column has powerful adsorption function, relatively strong specificity, high recovery rate and relatively low preparation cost, besides, improves the disadvantage of large required solvent amount in a traditional sample pretreatment technology, can be directly used for detection of ten sulfa veterinary drugs in animal-derived foods, and has the characteristics of high selective adsorption and high-efficiency enrichment on the sulfa drugs; and moreover, the solid-phase extraction column can be used for detection of sulfa veterinary drugs in a completely aqueous phase.

A gold-linked chromatography test paper box uses for analysis of the sulfonamides excessive remains. The test paper box makes up by plastic shell and test paper core, and the faceplate has sample adding hole and result displaying window. The reaction film is pyroxylin film with recessive test line and comparison line. Gold-linked combine pillow is fiberglass cotton with 2-5 strips mixed gold-linked antibody and the test line of the reaction film is 2-5 strips opposite sulfonamides-carrier albumen unite antigen. Or Gold-linked combine pillow is fiberglass cotton with 2-5 strips gold-linked sulfonamides-carrier albumen unite antigen, while the test line of the reaction film is 2-5 strips corresponding antibody to some sulfonamides. The comparison line of the reaction film is goat antirat orrabbit antirat IgG, or goat anti rabbit IgG, or rabbit-anti carrier albumen IgG. The box can test manifold sulfonamides at the same time and greatly save the test time with strong character, high sensitive, easy, intuitive, nicety and wide applied range, low cost for extending.

The invention relates to a method for determining the content of trimethoprim and various sulfonamides in a drug. The method comprises the following steps: preparing a mobile phase, determining chromatographic conditions, preparing a reference solution and a test solution, and determining the content. According to the method disclosed by the invention, the contents of trimethoprim, sulfadiazine, sulfathiazole, sulfadimidine, sulfamethoxydiazine, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfachloropyrazine and sulfaquinoxaline in the medicine can be determined, and a new method is provided for determining whether the preparation is adulterated or not; the method has the advantages of simple and rapid operation, accurate and reliable result, short determination time and the like.

The present invention provides an aqueous solution containing pranoprofen or a pharmacologically acceptable salt thereof, and a sulfa drug, and a method for making pranoprofen or a pharmacologically acceptable salt thereof stable to light in an aqueous solution, which includes adding a sulfa drug to the aqueous solution containing pranoprofen or a pharmacologically acceptable salt thereof.