This invention discloses a mixed broad-spectrum nucleic acidaptamer for the simultaneous detection of nine sulfonamide drugs and its applications. The mixed broad-spectrum nucleic acidaptamer is composed of nucleic acidaptamer sequences Sul-01 and Sul-04, which can be used as probes to construct a ratiometric fluorescence method for the simultaneous detection of nine sulfonamide drugs. The mixed broad-spectrum nucleic acid aptamer provided by this invention exhibits high specificity and high binding affinity for multiple sulfonamide drugs. The detection method established using it can achieve rapid, low-cost, and highly sensitive simultaneous detection of nine sulfonamide drug residues in aquatic environments and seafood.