The invention discloses nanometer wound-healing nursing gel and a preparation method thereof. The nanometer wound-healing nursing gel comprises a main material and an auxiliary material. The main material is prepared from Primula forrestii Balf. F., Viburnum cylindricum Buch.-Ham. ex D. Don, Veronica serpyllifolia L., Eleutherine plicata Herb., Winchia calophylla A. DC., Torenia vagans Roxb., anaphalis lacteal, Iris collettii Hook. F., fernleaf dichocarpum root, rongkol, mulberry wood sap, Berchemia kulingensis Schneid., halenia corniculata, Berberis atrocarpa Schneid., merremia hungaiensis, roughhair fig root, Pittosporum illicioides Makino, mysorethorn leaf, himalaya nightshade, haloragis micrantha, radix glycyrrhizae, erythrorchis altissima and red sand. The auxiliary material is prepared from nanometer crystalline cellulose, microfibril, glutamine, grape polyphenols, fibronectin, retinoic acid, anti-polymerization surface active agent, wetting agent and pH regulator. By means of the nanometer wound-healing nursing gel, healing of ulcer surfaces can be accelerated, scars are not prone to being left, and the prepared nanometer gel preparation is high in effective component content, high in absorption degree, free of chromatosis, convenient to use and free of dressing change and solves the problem that secondary harms are caused to wounds during dressing change.