High-frequency regeneration system method for iris ensata thumb on the basis of callus

A callus induction and high-frequency regeneration technology is applied in the fields of plant regeneration, horticultural methods, botanical equipment and methods, etc., and can solve the problems of inability to meet the needs of factory seedling raising, slow ramet propagation, and low multiplication coefficient, etc. Achieve the effect of high differentiation rate of sterile seedlings, increase reproduction speed, and expand reproduction coefficient

Active Publication Date: 2018-09-07
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES +1
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  • Abstract
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Problems solved by technology

[0004] The present invention aims at optimizing the regeneration system, and takes maintaining genetic stability as the core, and provides a method for establishing a high-frequency regeneration system based on callus induction, s

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  • High-frequency regeneration system method for iris ensata thumb on the basis of callus
  • High-frequency regeneration system method for iris ensata thumb on the basis of callus

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Embodiment Construction

[0015] The present invention is based on callus induction and establishes the method for high-frequency regeneration system of horsetail, comprising the following steps:

[0016] 1) Explant screening: select the leaf that the healthy plant of Iris japonica expands in spring, select the middle position of the leaf, and cut it off as an explant;

[0017] 2) Disinfection of explants: Put the leaves in step 1) in a 200mL beaker filled with clean water, add 2-3 drops of detergent, cover the mouth of the beaker with gauze, wash it with tap water, and add an appropriate amount of detergent Keep the flushing state at the tap water for 30-40 minutes, dry the water with filter paper; add 10% sodium hypochlorite solution with a mass percentage concentration in the beaker to completely soak the explant material, add 2 drops of Tween 20 (Tween20) to make disinfection After 15-20 minutes, pour out all the disinfectant in the beaker, pour the sterile water that has been sterilized by high te...

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Abstract

The invention relates to a high-frequency regeneration system method for an iris ensata thumb on the basis of a callus. The method takes spring iris lacteal pall leaves as an explant and takes MS (Murashige and Skoog) as a culture medium, and kinetin (KT), 2,4-dichlorphenoxyacetate (2,4-D), naphthylacetic acid (NAA), 6-benzyladenine (6-BA) and indolebutyric acid (IBA) hormone are used for establishing a method for establishing an iris ensata thumb high-frequency regeneration system on the basis of the callus. One mulitiplication period of the method is 4-5 weeks, callus inductivity is 80% or above, a callus adventitious bud induction coefficient is 8-10 times, breeding is not influenced by seasons, seedling breeding coefficients are greatly improved, original excellent characters are kept,a scientific and effective breeding method is provided for variety cultivation, application and production, and a reference can be provided for the industrialized production and breeding of iris plants.

Description

technical field [0001] The invention relates to a method for establishing a high-frequency regeneration system of stingrays based on callus induction. Background technique [0002] The white-flowered horse rush (Iris.lactea Pall.) that the present invention relates to is a perennial herbaceous flower of the genus Iris of the family Iridaceae, with basal leaves, gray-green, strip-shaped or narrow sword-shaped; milky white flowers, 5-6 cm in diameter, and 4-7 cm long pedicel The flowering period is 5-6 months long; salt-alkali and trampling-resistant, the rhizome is thick and the root system is developed, which can be used for water and soil conservation, improvement of saline-alkali soil and fragile ecological environment; Landscaping landscape materials with high ornamental value. [0003] However, due to the cold and long winter in Northeast China and the short growing season in summer, there are few types of plants available for urban greening, and the cost of greening is...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张兴曲彦婷王书可唐焕伟陈菲李黎韩辉熊燕石朔宇韩廷蔚陈颖张洪运李虹杨轶华孙波曲线
Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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