58results about How to "Excellent genetic traits" patented technology

The invention relates to a technology for propagating an aleurites moluccana seedling by high-altitude layering. According to the technology, an operating method comprises the following steps of: selecting branches of which the sizes are similar and the growth conditions are excellent from the aleurites moluccana tree which has more than 4 age from March to October in every year; performing girdling on the stem bases of the selected branches, wherein the width is about 2 to 4 centimeters, and a cortex layer and a cambium are scraped completely; coating a growth regulator with appropriate concentration at the upper ends of incisions; making a wet matrix into a mud pie, covering wounds with the mud pie, wrapping the outside of the mud pie by using a plastic film, and binding by using a packing rope; and checking the humidity of the mud pie during layering rooting, and when the humidity is low, replenishing moisture by an injection method to keep the humidity of soil, wherein after the girdling is performed, roots are grown at the wounds a month later; and cutting the layering for transplant when a large quantity of root systems are formed two or three month later.

The invention discloses a cutting and seedling method for phoebe shearer. The cutting and seedling method includes the steps: treating a cutting bed, namely paving cobblestones under a cutting bed and a layer of screen on the cutting bed and paving clean river sand for sterilization and disinfection of soil; collecting and producing cutting slips, namely clipping semi-lignification branches which are normal in growth with full shoots and without diseases and insect pests as cuttings from a phoebe shearer seed tree of 5-8 years from March to April to produce cutting slips; subjecting the produced cutting slips to early stage treatment and cutting; performing management after cutting. The cutting and seedling method is simple in operation, high in survival rate and capable of providing fine phoebe shearer seedlings, shortening breeding time of fine seeds and protecting excellent genetic traits of the seedlings. Therefore, propagation coefficient is greatly increased, and the survival rate reaches 80% at least.

The invention discloses a method for inducing gynogenetic diploid of grouper, which comprises the following steps of: diluting semen of the grouper, and radiating the semen by using ultraviolet rays to make the heritability of the semen deactivated; mixing with mature eggs of the grouper; after the eggs are fertilized, transferring to seawater for cold shock treatment to double genomes and generate the gynogenetic diploid; and transferring to seawater, and managing according to common grouper fry to obtain the gynogenetic diploid of the grouper. The offspring generated by induction only contains the gene of the female parent, the homozygosis of good genes can be quickened, and good hereditary features are quickly fixed; and the method can be applied to genetic analysis of the grouper, and has wide application prospect in the aspects of culture and breeding of the grouper.

A method for inducing the gynogenesis diploid of large yellow croaker includes such steps as UV irradiating to the diluted sperm of large yellow croaker to deactivate its heredity, mixing it with the ripe ova, starting the gynogenesis of ova, transferring the inseminated ova to seawater, cold shock or static water pressure treating to generate gynogenesis diploid, transferring the inseminated gynogenesis ova in seawater, and ordinary management to obtain the gynogenesis diploid fry.

The invention discloses a factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium. The method comprises the following steps: by taking a flower bud of dendranthema morifolium as an explant, inducing dedifferentiation and redifferentiation; obtaining the test-tube seedlings through a tissue culture technique; carrying out transitional planting in a vermiculite matrix or a matrix with a ratio of peat to vermiculite to perlite being 33% to 33% to 33% to obtain a shoot with 3-5 leaves and height of 15-20cm after about 30 days; and transplanting to a land for growing field crops, thus harvesting in the current year. By adopting the technical scheme, the seedlings are fully originated from a same plant which accords with inheritable character, so that virus accumulation is reduced, the period is short and the effect is achieved fast. Genuine medicinal material dendranthema morifolium is convenient to store and quick to popularize as a conventional resource, and the method is an effective method suitable for factory-like production of the test-tube seedlings.

The invention discloses an asexual propagation method for a good mangifera indica variety for greening. The method comprises the procedures of maternal plant selecting, propagating material acquiring, cutting collecting, cutting pruning, rooting solution preparing, cuttage and post-cuttage management, i.e., selecting the good mangifera indica variety for greening as cutting collecting maternal plants, girdling the cutting collecting maternal plants to obtain bud sticks which have the length of 40cm to 60cm and are robust in growth and free from plant diseases and insect pests, collecting and pruning the bud sticks, then, soaking the bud sticks in a rooting solution, then, carrying out cuttage, and then, carrying out post-cuttage management, thereby obtaining mangifera indica cuttage seedlings for greening. According to the asexual propagation method for the good mangifera indica variety for greening, variety screening is carried out according to selective breeding requirements of garden tree species, the excellent mangifera indica variety applicable to landscaping cultivation is screened out, the traditional purposeless and unordered greening mangifera indica breeding cultivation habit is changed, the commodity value of greening mangifera indica is increased, and the market demands are met.

The invention discloses a method for hybridized cultivation of big floral Chinese roses. The method is characterized by comprising the following steps: (1) pollen preservation: selecting bright-colored, and big Chinese roses, collecting the pollen of the luxuriant Chinese roses by using a sterile conical flask, baking the pollen, and then preserving the baked pollen; (2) activation: performing preservation in the environment with a temperature of 18-20 DEG C, and performing atomization treatment by using activation liquid; (3) pollination treatment: spraying 75% ethanol to the big floral Chinese roses so as to be sterilized, performing pollination, parcelling the pollinated Chinese roses with a transparent plastic bag, then spraying the activation liquid, and performing bagging for culturing; (4) hybridization: performing pollination hybridization respectively on the pollen of the luxuriant Chinese roses and the pollen of the big floral Chinese roses with hybridized big floral Chineseroses; and (5) intraspecific breeding: putting the fourth generation of seeds in a greenhouse so as to be planted at a constant temperature, and performing intraspecific pollination after blooming.

The invention provides a rapid breeding method of Euryodendron excelsum cultivation seedlings. The method comprises the steps of explant selection and disinfection, aseptic seedling culture, clustered bud induction, rooting culture, seedling strengthening culture and seedbed culture. Compared with traditional seedling growing methods of Euryodendron excelsum, the method provided by the invention has the advantages of small amount of needed Euryodendron excelsum seeds, realization of short-time mass propagation, keeping of excellent hereditary features of Euryodendron excelsum, good quality of seedlings, low malformed seedling rate, high survival rate of the seedlings reaching 90%, high survival rate of cultivated seedlings reaching 80%, good growth vigor of transplanted nursery stocks, no runt seedlings, effective solving of the problems of increasing reduction of wild resources and difficult natural population updating of Euryodendron excelsum, and provision of technical support for effective protection of the rare and endangered plant Euryodendron excelsum.

The invention discloses a tissue culture fast propagation method of Eucalyptus pellita. The method comprises the following steps: adopting excellent single plants of an excellent family of Eucalyptus pellita, carrying out stump sprouting on the excellent single plants, carrying out in vitro aseptic bud induction on stems with buds through using obtained sprouts as an explants to directly generate lateral buds or adventitious buds without a callus approach, carrying out shoot proliferation culture on the aseptic buds, carrying out rooting culture, and transplanting rooted seedlings to obtain regenerated plants. The tissue culture fast propagation method of Eucalyptus pellita has the advantages of low operating difficulty, low production cost, high rooting rate (of 90% or above), high survival rate of produced tissue culture plants, and easy large-scale popularization and use, and the regenerated plants obtained by using the method have the advantages of stable result, good repeatability, realization of large-scale popularization and seedling growing, stable hereditary character, and maintenance of excellent hereditary characters of excellent trees.

The invention provides a method for screening a high-content functional component dendrobe culture, a method for improving polysaccharide and/or dendrobine in a dendrobe tissue culture seedling and a kit for implementing the methods. The method disclosed by the invention comprises: a seed pretreatment step: processing sterile dendrobe seeds by using NaCl and/or mannitol; a seed induced germination step: cultivating the seeds in an induced culture medium containing NaCl and/or mannitol, so as to form a protocorm after the seeds germinate; an induced proliferation step of embryo callus: cultivating the protocorm by using callus proliferation medium, and forming a cell mass or callus, so as to obtain the dendrobe tissue culture. The kit disclosed by the invention contains various culture mediums for implementing the methods disclosed by the invention. By adopting the methods disclosed by the invention and the kit, tissue culture seedlings with healthy growth and vigorous metabolism can be obtained; the alkali content of main functional components, namely polysaccharide and dendrobe is higher than that of a common tissue culture seedling; the dendrobe culture can be stably inherited, and can serve as an excellent seedling breeding source.

The invention discloses a grafting method correcting crown deflection of bombax ceiba grafted seedlings. A robust bombax ceiba seedling with one or more rings of branches is selected and used as a stock, and a robust and upright bombax ceiba branch bud which is equivalent to the branches on the stock in thickness is selected and used as scion; the slope cut-grafting method is used for enabling a cambium and phloem of a scion slope to be aligned and connected with cambiums and phloem of slopes of the branches of the stock in a sealed mode, and the joints are bound and fixed through plastic films. The specific slope cut-grafting method is used for carrying out grafting on different branches of the first ring of branches of the stock, the area of grafting operation of the stock is large, survival of grafted seedlings is not affected, and the survival rate of the grafted seedlings can reach 96.67%. Due to the fact that branches in different directions are grafted, balanced and equal growth of all branches is more facilitated, crown deflection is avoided, the grafted seedlings has the better ornamental trait, potential safety hazards are avoided, and the grafting method can be widely applied to gardens and parks.

The invention belongs to the field of functional red yeast rice health-preserving beverages, and particularly relates to a preparation method of highland barley functional red yeast rice health-preserving tea. According to the preparation method of the highland barley functional red yeast rice health-preserving tea, the adopted functional red yeast rice is monascus (Monascus sp.) induced by mutant strains through an SJ-10 spacecraft, and the microbial preservation number of the monascus is CGMCCNO.13773. The preparation method of the health-preserving tea disclosed by the invention comprises the following steps of cleaning highland barley and cooking the cleaned highland barley; preforming acid treatment; performing enzymolysis; preparing materials; performing sterilization; and performing inoculating and performing fermentation culturing so as to obtain the highland barley functional red yeast rice. The preparation method disclosed by the invention has the beneficial effects that physiological activators generated in metabolism and accumulation of the functional red yeast rice and massive nutrient substances contained in the highland barley are combined, so that the highland barley red yeast rice tea has the efficacy of reducing blood pressure, reducing cholesterol and the like, and has obvious prevention effects on diseases of high blood lipid, high blood pressure, diabetes and the like.

The invention belongs to the technical field of plant propagation and particularly relates to a cutting propagation method of Phoebe sheareri. The cutting propagation method is implemented in a container and specifically comprises steps as follows: (1) selection of a cutting container and preparation of a cutting matrix: the cutting matrix is prepared from raw materials as follows: 80-100 parts of peat, 30-50 parts of perlite, 80-100 parts of saw dust, 20-30 parts of dry branches and fallen leaves, 30-50 parts of farmyard manure, 10-20 parts of nitrogen-fixing bacteria manure, 10-15 parts of phosphate-solubilizing microorganisms and 5-10 parts of EM (effective microorganism) fertilizer; (2) root-divided cutting: fresh roots with the diameters being 2-5 cm are selected and cut into short shoots which are 15-18 cm long, and fine root ends are soaked in a 50 mg/kg naphthalene acetic acid solution for 3-3.5 h; (3) the treated short shoots are cut in the container. By means of the cutting propagation method of the Phoebe sheareri, the survival rate is 85% or higher, the genetic character of the nursery stock is excellent, the operation is simple, and the seedling culture time is shortened.

The invention discloses a tajsho-sanke reproducing and breeding method. The method comprises the steps of parent fish selection, intensive parent fish culture, parent fish egg laying, fish fry rearing, primary optimization, secondary optimization and tertiary optimization. High-quality parent fish with complete fin rays, bright body color and obvious varietal characteristics is selected, a good foundation is laid for good inheritable characters of offspring fries, on the basis of screening of tajsho-sanke, finally, the fish bodies with the good strain, healthy, strong and beautiful body type, bright color, good muscle and obvious varietal characteristics are finally selected, after three levels of optimization, and the good-quality tajsho-sanke with the yield reaching up to 7% is selected.

The invention discloses a tabebuia chrysantha tissue culture and rapid propagation method. The tabebuia chrysantha tissue culture and rapid propagation method comprises the steps of treatment and disinfection of an explants, acquisition of aseptic seedlings, differentiation and proliferation of aseptic buds and culture and transplanting of rooting seedlings. A propagation method and a culture medium formula of the invention are used for carrying out tabebuia chrysantha tissue culture, the annual growth coefficient of the aseptic buds reaches 3.012, and the rooting rate reaches 97 percent. The tabebuia chrysantha tissue culture and rapid propagation method is provided by using tabebuia chrysantha seeds as the explants, an effective path is provided for seedling breeding of tabebuia chrysantha, and large-scale production requirements of the seedling breeding are met.

The invention provides an inducing method for a gynogenetic diploid of a Nibea albiflora, comprising the following steps of: (1) carrying out genetic inactivation on Nibea albiflora or Oplegnathus fasciatus sperms; (2) inseminating unfertilized ovums of the Nibea albiflora and inactivated sperms; (3) determining the starting time of chromosome reduplication of gynogenetic roes; and (4) screening the cold shock temperature and the duration of chromosome reduplication. The invention firstly provides a method for inducing Nibea albiflora ovum gynogenesis by using the sperms of the Nibea albiflora and the sperms of the Oplegnathus fasciatus to obtain a gynogenetic diploid fish fry, and the proper cold shock starting time, the cold shock temperature and the duration for Nibea albiflora ovum gynogenesis are screened out. The inducing method has the characteristics of convenience in operation, high efficiency, practicability and reliability; and a Nibea albiflora progeny obtained through inducing by using the method only contains the genetic materials of a female parent, so that the purification of an excellent gene can be accelerated, the excellent hereditary character can be rapidly fixed, and the inducing method has an important application value and a favorable popularization and application prospect on the aspect of breeding and sex control research of the Nibea albiflora.

The invention provides a cuttage planting method of carpinus turczaninowii and relates to the technical field of plant cultivation. The planting method includes: selecting cuttage wood, treating the cuttage wood, treating nursery land, and performing cuttage. The cuttage planting method is low in cost, simple to operate and quick in propagation, obtained seedlings are large in number, has excellent and stable hereditary character, and the seedlings after cuttage are uniform in growing trend and high in survival rate.

The invention discloses a cutting breeding method for osmanthus trees and belongs to the field of plant breeding. The method comprises the following steps: (1) collecting and treating cutting slips, namely collecting twigs from stock plants to serve as the cutting slips, cutting along the axial direction of larger ends of the twigs by using a cutter so as to form a cut, and performing rooting treatment; (2) performing seedling bed arrangement, namely arranging a seedling bed in a greenhouse, and spreading cutting media on the seedling bed; (3) performing cutting, namely inserting the cutting slips into the cutting medium layer, compacting and watering; (4) performing seedling stage management, namely watering with a nutrient solution once every 5-7 days when roots grow from the cut of the larger ends of the twigs, and maintaining corresponding temperature and humidity in the greenhouse; and (5) transplanting, namely transplanting within 2-3 months after cutting, thereby obtaining the seedlings. The cutting breeding method for osmanthus trees disclosed by the invention is simple in operation, the survival rate, seedling quality and emergence rate of cuttings of osmanthus trees can be effectively improved, the production cost is reduced, and the economic benefits are improved.

The invention discloses a tissue culture rapid propagation method of eucalyptus fine-breed urophylla*E.camaldulensis DH191-7. Stump germination promotion is performed on a superior tree of a DH191-7 clone, and a stem segment of a sprouting band latent bud is used as an explant to perform induction in vitro on a germ-free bud. A side bud is sprouted directly from the stem segment without a callus tissue, and subculture multiplication cultivation, rooting cultivation and rooted seedling transplantation are performed on the germ-free bud to obtain a regeneration plant. As the eucalyptus fine breed is used as a reproduction target, and seedlings are directly formed in the way of performing induction in vitro on the germ-free bud by a bud organ, the fine hereditary feature of the superior tree can be completely kept in the regeneration plant, and the regeneration plant is not easy to mutate; the method is low in operation difficulty, low in production cost, high in nursery stock root yield, high in transplantation survival rate, stable in result and good in repeatability; and the method can be used for massive industrialized seedling production.

The invention provides a semi-hydroponics cutting rooting method for lyceum ruthenicm murr, belonging to the technical field of cutting of lyceum ruthenicm murr. The method comprises the following steps of (1) oscillating twigs of lyceum ruthenicm murr for 7-9h at 26-30 DEG C, treating the oscillated twigs for 0.5-1.5h at low temperature of 6-9 DEG C so as to obtain to-be-plant twigs; (2) inserting the to-be-plant twigs into a matrix for rooting for 40-50d so as to obtain field transplanted seedlings, wherein a rooting solution is irrigated into the matrix in rooting process, and the mass to volume ratio (g/ml) of the matrix to the rooting solution is kept at 1:(2-3). Through adoption of the method, the rooting rate of the planted twigs of lyceum ruthenicm murr can reach 100%, the roots grow strongly, and 40-50d later, field transplanted seedlings can be obtained; and the method is easy to operate, the seedling culture period is short, the seedlings growing speed is high, so that the method can be applied to industrial cultivation of lyceum ruthenicm murr seedlings.

The invention relates to a tissue culture rapid-propagation method of Maoba wild sumac. Fresh axillalry buds and terminal buds of five-year excellent Maoba wild sumac are used as explant culture materials and undergo steps of disinfection, induced culture, proliferation culture, rooting culture, hardening-seedling, transplantation and the like. Rapid propagation of Maoba wild sumac is realized through the tissue culture rapid-propagation technology. For each 10 Maoba wild sumac explants, propagation coefficient is 6.5, and propagation cycle is 30 days. Except pollution loss, yearly propagation rate reaches 2.75 million and above. Thus, the problem that Maoba wild sumac is scarce is effectively solved, and the requirement of large-area rapid propagation is met. By accurate selection of explant picking time and tree-age, induced differentiation rate of adventitious buds is raised, and the differentiation rate reaches 85% and above. In addition, browning rate of explants is lower than 8%, pollution rate is lower than 2%. By adoption of closed hardening-seedling and reasonable matrix proportion, survival rate of seedlings is greatly raised, and the survival rate is maintained at 90% and above.

The invention discloses an explant collecting and inducing method of leaf buds of betula alnoides, belonging to the technical field of vegetative propagation of plants. The method comprises the following steps of: (1) selecting a betula alnoide superior tree of above five years old as a seed tree; (2) in August-October, clearing weeds and bushes around the seed tree in the diameter range of 5m to ensure that the tree trunk is fully illuminated by sunlight, preparing a 6-benzyladenine solution with the concentration of 5mg/L simultaneously and transfusing the solution into the cambium of the tree trunk base for more than 30 days; (3) in October-December, scissoring and taking 2-3cm long healthy and strong leaf bud produced at 2m below the tree trunk base as the explant, soaking for 20min by using 3% of washing powder, disinfecting the explant for 10-15s by using 75% of alcohol on a super-clean worktable, disinfecting the explant for 7-10min by using 10% of H2O2 and finally washing 5-7 times by using sterile water; and (4) transferring the leaf bud to an induction medium of the explant and culturing for 40-60 days, wherein the induction success rate is more than 50%.