Method for inducing gynogenetic diploid of Pseudosciaena crocea

A technology of gynogenesis and large yellow croaker, applied in the field of gynogenesis, to achieve the effect of accelerating homozygosity

Inactive Publication Date: 2006-06-28
JIMEI UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on gynogenesis of large yellow croaker is still blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the semen of large yellow croaker and dilute it 30 times with the diluent, put it in a petri dish with a diameter of 6-15cm, and irradiate it with ultraviolet rays. The ultraviolet irradiation dose is 50000-100000μW / cm 2 , semen thickness 0.5mm. The preparation of the diluent is: each liter of solution contains 7.5g of NaCl, 0.2g of KCl, and 0.2g of CaCl 2 0.2g, NaHCO 3 0.2g, to inactivate the genetics of sperm. Adjust the seawater temperature to 22-23°C, mix mature eggs of large yellow croaker and sperm of large yellow croaker irradiated by ultraviolet rays in the sea water, and the final concentration of sperm should be at least 10 5 individual / ml to start the gynogenesis of large yellow croaker eggs. 3 to 4 minutes after fertilization, the eggs were transferred to seawater at 0 to 1°C for cold shock for 10 minutes, so that the gynogenetic haploid chromosome group of large yellow croaker was doubled to become gynogenetic diploid. The gynogenetic eggs of lar...

Embodiment 2

[0029] Similar to Example 1, the difference is that the large yellow croaker semen is diluted 10 times with the diluent and then irradiated with ultraviolet rays, and the ultraviolet irradiation dose is 50000-100000 μW / cm 2 , Semen thickness 0.8mm. The preparation of the diluent is: each liter of solution contains 7.5g of NaCl, 0.2g of KCl, and 0.2g of CaCl 2 0.2g, NaHCO 3 0.2g, to inactivate the genetics of sperm. Adjust the seawater temperature to 20-22°C, mix mature eggs of large yellow croaker and sperm of large yellow croaker irradiated by ultraviolet rays in the sea water, and the final concentration of sperm should be at least 10 5 individual / ml to start the gynogenesis of large yellow croaker eggs. 4 to 5 minutes after fertilization, the eggs are transferred to a hydraulic press for hydrostatic treatment at a pressure of 45MPa / cm 2 , The time for hydrostatic treatment in the hydraulic press is 2 to 3 minutes. Doubling the gynogenetic haploid chromosome set of la...

Embodiment 3

[0031] Similar to Example 1, the difference is that the large yellow croaker semen is diluted 50 times with the diluent and then irradiated with ultraviolet rays, and the ultraviolet irradiation dose is 50000-100000 μW / cm 2 , semen thickness 1.0mm. The preparation of the diluent is: each liter of solution contains 7.5g of NaCl, 0.2g of KCl, and 0.2g of CaCl 2 0.2g, NaHCO 3 0.2g, the genetic inactivation of sperm. Adjust the seawater temperature to 23-25°C, mix mature eggs of large yellow croaker and sperm of large yellow croaker irradiated by ultraviolet rays in the sea water, and the final concentration of sperm should be at least 10 5 per ml to start the gynogenesis of large yellow croaker eggs. 4 minutes after fertilization, the eggs are transferred to a hydraulic press for hydrostatic treatment at a pressure of 45MPa / cm 2 , the time of hydrostatic treatment in the hydraulic press is 3min. Doubling the gynogenetic haploid chromosome set of large yellow croaker into g...

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Abstract

A method for inducing the gynogenesis diploid of large yellow croaker includes such steps as UV irradiating to the diluted sperm of large yellow croaker to deactivate its heredity, mixing it with the ripe ova, starting the gynogenesis of ova, transferring the inseminated ova to seawater, cold shock or static water pressure treating to generate gynogenesis diploid, transferring the inseminated gynogenesis ova in seawater, and ordinary management to obtain the gynogenesis diploid fry.

Description

technical field [0001] The invention relates to a method for gynogenesis, in particular to a method for inducing diploid gynogenesis in large yellow croaker. Background technique [0002] The use of cell engineering technology to cultivate fine varieties for aquaculture has far-reaching theoretical significance and broad application prospects. Gynogenesis is a kind of cell engineering technology, which refers to the phenomenon that the egg develops into an individual only by its own genetic material. It needs the same or different sperm to enter the egg, but these sperm only play an exciting role and do not participate in the development. The development is completely under the control of the gynogenetic nucleus, so the offspring obtained are all maternal traits, and the genotype is the same or slightly different from the female parent. Artificial induction of gynogenesis is one of the most effective methods to accelerate the homozygosity of good genes in fish, so that good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00A01K61/00
CPCY02A40/81
Inventor 王志勇谢芳靖刘家富
Owner JIMEI UNIV
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