Indoor population propagation method for lasioderma serricorne parasitic wasps

Abstract

The invention provides an indoor population propagation method for Lasioderma serricorne parasitic wasps, which comprises the following steps: (1) Lasioderma serricorne adults are put into a feeding container, and are fed with artificial feed; (2) the lasioderma serricorne parasitic wasps are inoculated into the feeding container for feeding and then released; the parasitic wasp of the lasioderma serricorne comprises scleroderma guani. According to the method provided by the invention, obligate diapause of the lasioderma serricorne can be broken, large-scale rapid propagation of the lasioderma serricorne is realized, large-scale propagation of a scleroderma guani population is further realized, the cultivation efficiency is greatly improved, the survival rate and the propagation rate of parasitic wasps are improved, and the overall cost benefit is improved.

Description

Technical Field

[0001] The invention belongs to the field of biological control, and specifically relates to an indoor population expansion method of a tobacco beetle parasitic wasp, and in particular to an indoor population expansion method of a tobacco beetle parasitic wasp with a high survival rate. Background Art

[0002] The tobacco beetle Lasioderma serricorne belongs to the family of Coleoptera and is an important storage pest in the world's tobacco storage industry. The quality loss of tobacco leaves caused by tobacco-boring pests accounts for about 1.64% of the total amount of stored tobacco leaves each year, and the loss caused by tobacco beetles accounts for more than 98% of the total loss. The amount of tobacco losses caused by tobacco beetles worldwide exceeds US$300 million. The swelllegged wasp of the head of the galaxyla is a parasitic natural enemy of tobacco beetles. It has a wide range of survival and has been reported both at home and abroad. Its host range includes storage beetles such as Anobiidae, Bostrychidae, Buprestidae, Lyctidae, Ciidae, and Cucujidae. With the development of the economy and the improvement of people's environmental awareness, chemical control methods are becoming increasingly limited. Biological control is an important research direction for the prevention and control of tobacco beetles in the future. In tobacco storage warehouses in some areas, aluminum phosphide fumigation and other methods are no longer allowed to control tobacco beetles. The Scleroderma gellata is a specialized parasitic enemy of stored product beetles. Currently, research on the wasp is relatively limited worldwide.

[0003] Therefore, how to provide a method that can effectively expand the population of the scleroderma has become an urgent problem to be solved. Summary of the invention

[0004] In view of the shortcomings of the prior art, the purpose of the present invention is to provide an indoor population expansion method for tobacco beetle parasitic wasps, and in particular to provide an indoor population expansion method for tobacco beetle parasitic wasps with a high survival rate. The method provided by the present invention can break the specialized diapause of tobacco beetles, achieve rapid reproduction of tobacco beetles in large quantities, and then achieve large-scale expansion of the population of Scleroderma gellativum, greatly increase the breeding efficiency, improve the survival rate and reproduction rate of parasitic wasps, and improve the overall cost-effectiveness.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] The present invention provides an indoor population expansion method of a tobacco beetle parasitic wasp, and the indoor population expansion method comprises the following steps:

[0007] (1) placing adult tobacco beetles in a breeding container and feeding them with artificial feed;

[0008] (2) placing tobacco beetle parasitic wasps into a breeding container for breeding and then releasing them;

[0009] The tobacco beetle parasitic wasps include Scleroderma gellativum.

[0010] The above method can break the obligate diapause of tobacco beetles, realize large-scale rapid reproduction of tobacco beetles, and then realize large-scale expansion of the population of Swellleaf wasp, eliminating the work of host selection and parasitic wasp collection, reducing manual labor hours and experimental sample loss caused by manual operations, greatly increasing breeding efficiency, combining breeding and release work, improving the survival rate and reproduction rate of parasitic wasps, and improving overall cost-effectiveness.

[0011] Preferably, the artificial feed in step (1) comprises any one of wheat, oats, yeast powder or porous starch, or a combination of at least two of them, preferably a combination of oats, yeast powder and porous starch.

[0012] The above-mentioned specific artificial feed can effectively prevent the degeneration of tobacco beetles after iterative breeding, which would affect the expansion and reproduction of the population of the Gellata head beetle.

[0013] Preferably, the mass ratio of oats, yeast powder and porous starch is (15-25):(0.8-1.2):(3-7), wherein the number of oats can be 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25, etc., the number of yeast powder can be 0.8, 0.9, 1, 1.1 or 1.2, etc., and the number of porous starch can be 3, 4, 5, 6 or 7, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are equally applicable.

[0014] Preferably, the breeding container in step (1) is in an environment with a temperature of 24-26°C.

[0015] Preferably, the breeding container in step (1) is in an environment with a relative humidity of 65-75%.

[0016] Preferably, the breeding container in step (1) is in an environment with a light cycle of (14-18) L: (6-10) D.

[0017] The temperature can be 24°C, 24.5°C, 25°C, 25.5°C or 26°C, etc., the relative humidity can be 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74% or 75%, etc., and the photoperiod can be 14L:10D, 15L:9D, 16L:8D, 17L:7D, 18L:6D, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0018] Preferably, the breeding container in step (1) comprises a breeding dish, a blocking net arranged at an opening of the breeding dish, and a release cover arranged above the blocking net.

[0019] Preferably, the mesh number of the barrier net is not less than 80 meshes.

[0020] Preferably, the release cover is provided with a vent hole.

[0021] The above-mentioned specific breeding device can integrate the cultivation and release work, and is adapted to the characteristics of the galaxysphaeria, effectively improving the survival rate and reproduction rate of the parasitic wasp.

[0022] Preferably, the feeding time in step (1) is 40-45 days.

[0023] Preferably, in the tobacco beetle parasitic wasp introduced in step (2), the ratio of the number of wingless adult bees to that of winged adult bees is (7-9):1, for example, 7:1, 7.5:1, 8:1, 8.5:1 or 9:1, but is not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0024] Preferably, after the step (2) of inoculating the tobacco beetle parasitic wasp for breeding, the method further comprises placing a cotton ball soaked in honey water on top of the barrier net.

[0025] Preferably, in the honey water, the volume ratio of honey to water is 1:(8-12), such as 1:8, 1:9, 1:10, 1:11 or 1:12, but is not limited to the above-listed values, and other unlisted values ​​within the above numerical range are also applicable.

[0026] Preferably, the cotton ball is replaced every 1-2 days.

[0027] The above-mentioned specific honey water can supplement nutrition for parasitic bees and prolong their lifespan.

[0028] Preferably, the feeding time in step (2) is 12-15 days.

[0029] Preferably, before the release in step (2), the step also includes removing the barrier net and the release cover of the breeding device, and placing the breeding device at the release location.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention provides an indoor population expansion method for tobacco beetle parasitic wasps, which can break the specialized diapause of tobacco beetles, realize large-scale rapid reproduction of tobacco beetles, and further realize large-scale expansion of the population of Scleroderma galerata, eliminate the host selection and parasitic wasp collection work links, reduce manual labor hours and experimental sample loss caused by manual operation, greatly increase the breeding efficiency, integrate the breeding and release work, improve the survival rate and reproduction rate of the parasitic wasps, and improve the overall cost-effectiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 is a front structural schematic diagram of the breeding container in Example 1;

[0033] Figure 2 It is a schematic diagram of the longitudinal cross-section structure of the breeding container in Example 1. DETAILED DESCRIPTION

[0034] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0035] Example 1

[0036] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps, using a breeding container such as Figure 1-2 1 is a bottle cap, 4 is a glass dish cover body, the bottle cap is fixed on the glass dish cover body through a threaded groove 3, 5 is an 80-mesh mesh, the glass dish cover body and the 80-mesh mesh below the bottle cap are provided with an air hole 2 (aperture 0.6mm), the glass dish cover body and the 80-mesh mesh outside the bottle cap are provided with a feed hole 8 (aperture 0.8mm), 7 is a glass feeding dish, and is fixed to the glass dish cover body through a buckle 6.

[0037] The specific method is as follows:

[0038] (1) Preparation of artificial feed: The mass ratio of the artificial feed formula is oatmeal: yeast powder: porous starch = 20:1:5. The oats are crushed with a universal grinder and passed through a 50-mesh sieve. After sieving, the oatmeal powder is mixed with yeast powder and porous starch according to the above formula, and put into an 8cm×8cm×12cm glass jar, which is placed in an autoclave and sterilized at 121°C for 15 minutes. After natural cooling, it is taken out and waited for use.

[0039] (2) Adult tobacco beetles were placed in glass culture dishes and placed in an artificial climate chamber (ambient temperature 25±1°C, relative humidity 70%, photoperiod 16L:8D) for 40 days.

[0040] (3) Inoculate Scleroderma gellativum into a glass culture dish, where the ratio of adult tobacco beetles, wingless adult bees, and winged adult bees is 200:8:1; prepare honey water with a volume ratio of honey to water of 1:10, soak a cotton ball in it (slightly squeeze it until the liquid drips out), and then place it on the feeding hole.

[0041] (4) After 12 days of inoculation of the Scleroderma gracile, remove the 80-mesh screen and bottle cap, and when the Scleroderma gracile gradually emerges in the rearing container, take the rearing container to the release site.

[0042] Example 2

[0043] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps, using a breeding container such as Figure 1 shown.

[0044] The specific method is as follows:

[0045] (1) Preparation of artificial feed: The mass ratio of the artificial feed formula is oatmeal: yeast powder: porous starch = 20:1:5. The oats are crushed with a universal grinder and passed through a 50-mesh sieve. After sieving, the oatmeal powder is mixed with yeast powder and porous starch according to the above formula, and put into an 8cm×8cm×12cm glass jar, which is placed in an autoclave and sterilized at 121°C for 15 minutes. After natural cooling, it is taken out and waited for use.

[0046] (2) Adult tobacco beetles were inoculated into a breeding dish and placed in an artificial climate chamber (ambient temperature 25±1°C, relative humidity 70%, photoperiod 16L:8D) for 45 days.

[0047] (3) Inoculate Scleroderma gellata into a breeding dish, where the ratio of adult tobacco beetles, wingless adult bees, and winged adult bees is 200:7:1; prepare honey water with a volume ratio of honey to water of 1:8, soak a cotton ball in it (slightly squeeze it until the liquid drips out), and then place it on the blocking net.

[0048] (4) After 12 days of inoculation of the Scleroderma gellata, remove the blocking net and wait for the Scleroderma gellata to emerge in the breeding container, then take the breeding container to the release site.

[0049] Example 3

[0050] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps, using a breeding container such as Figure 1 shown.

[0051] The specific method is as follows:

[0052] (1) Preparation of artificial feed: The mass ratio of the artificial feed formula is oatmeal: yeast powder: porous starch = 20:1:5. The oats are crushed with a universal grinder and passed through a 50-mesh sieve. After sieving, the oatmeal powder is mixed with yeast powder and porous starch according to the above formula, and put into an 8cm×8cm×12cm glass jar, which is placed in an autoclave and sterilized at 121°C for 15 minutes. After natural cooling, it is taken out and waited for use.

[0053] (2) Adult tobacco beetles were inoculated into a breeding dish and placed in an artificial climate chamber (ambient temperature 25±1°C, relative humidity 70%, photoperiod 16L:8D) for 40 days.

[0054] (3) Inoculate Scleroderma gellata into a breeding dish, where the ratio of adult tobacco beetles, wingless adult bees, and winged adult bees is 200:9:1; prepare honey water with a volume ratio of honey to water of 1:12, soak a cotton ball in it (slightly squeeze it until the liquid drips out), and then place it on the blocking net.

[0055] (4) After 15 days of inoculation of the Scleroderma gellata, remove the blocking net and wait for the Scleroderma gellata to emerge in the breeding container, then take the breeding container to the release site.

[0056] Example 4

[0057] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with Example 1 except that the ratio of the number of wingless adult bees to winged adult bees is 6:1.

[0058] Example 5

[0059] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with Example 1 except that the ratio of the number of wingless adult bees to winged adult bees is 10:1.

[0060] Example 6

[0061] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with Example 1 except that honey is replaced with an equal amount of pollen solution.

[0062] Example 7

[0063] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with Example 1 except that the volume ratio of honey to water is 1:7.

[0064] Example 8

[0065] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with Example 1 except that the volume ratio of honey to water is 1:13.

[0066] Example 9

[0067] The present embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasp. Except for using the breeding device in CN202110135176.8 for breeding, the rest of the specific method is consistent with Example 1.

[0068] Example 10

[0069] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasp. The specific method is consistent with Example 1 except that oats are replaced with an equal amount of wheat in the artificial feed.

[0070] Embodiment 11

[0071] This embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with that of Example 1 except that yeast powder is not added to the artificial feed and a reduced portion is allocated in proportion to porous starch and oats.

[0072] Example 12

[0073] The present embodiment provides a method for indoor population expansion of tobacco beetle parasitic wasps. The specific method is consistent with that of Example 1 except that porous starch is not added to the artificial feed and a reduced portion is allocated in proportion to yeast powder and oats.

[0074] Effect test:

[0075] The survival time and number of offspring of the parasitic wasps obtained by the methods of Examples 1-9 were statistically analyzed (four repeated tests were performed in each group, and the four test results were analyzed for variance using SPSS), and the results were as follows:

[0076] Group Survival time (d) Number of offspring (only) Group Survival time (d) Number of offspring (only) Example 1 14.32 140.46 Example 6 14.91 138.13 Example 2 14.85 131.19 Example 7 14.43 131.11 Example 3 13.48 133.07 Example 8 13.26 125.43 Example 4 13.93 117.61 Example 9 13.68 137.71 Example 5 14.08 124.33

[0077] From the above data, it can be found that the method provided by the present invention can effectively realize the indoor propagation of Scleroderma galerata; at the same time, by comparing Examples 1-9, it can be found that the present invention effectively improves the survival rate and reproduction rate of parasitic bees by adopting honey water, controlling relevant parameters and adopting specific breeding containers, thereby improving the overall cost-effectiveness (the price of pollen liquid is more than 10 times higher than that of honey).

[0078] Then, the method of Examples 1, 10-12 was used to iteratively breed the tobacco beetles (for five generations) and observe the degeneration of the tobacco beetles. The statistics are as follows:

[0079] Example 1 The average length of the adult is 2.87 mm and the width is 2.53 mm. Example 10 The average length of the adult is 2.85 mm and the width is 2.51 mm. Embodiment 11 The average length of the adult is 2.72 mm and the width is 2.48 mm. Example 12 The average length of the adult is 2.77 mm and the width is 2.49 mm.

[0080] From the above data, it can be found that the present invention can effectively prevent the tobacco beetles from degenerating (i.e., body length becomes shorter and body width becomes narrower) after iterative breeding by selecting specific artificial feed, thereby effectively ensuring the long-term use of the method of the present invention.

[0081] The applicant declares that the present invention illustrates the indoor population expansion method of the tobacco beetle parasitic wasp of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0082] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0083] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for indoor population expansion of tobacco beetle parasitic wasps, characterized in that: The indoor population expansion method comprises the following steps: (1) placing adult tobacco beetles in a breeding container and feeding them with artificial feed; (2) placing tobacco beetle parasitic wasps into a breeding container for breeding and then releasing them; The tobacco beetle parasitic wasps include Scleroderma gellativum.

2. The indoor population propagation method according to claim 1, characterized in that: The artificial feed in step (1) comprises any one of wheat, oats, yeast powder or porous starch, or a combination of at least two of them, preferably a combination of oats, yeast powder and porous starch.

3. The indoor population propagation method according to claim 2, characterized in that: The mass ratio of the oats, yeast powder and porous starch is (15-25):(0.8-1.2):(3-7).

4. The indoor population propagation method according to any one of claims 1 to 3, characterized in that: The breeding container in step (1) is placed in an environment with a temperature of 24-26° C., a relative humidity of 65-75%, and a photoperiod of (14-18) L:(6-10) D.

5. The indoor population propagation method according to any one of claims 1 to 4, characterized in that: The breeding container in step (1) comprises a breeding dish, a blocking net arranged at an opening of the breeding dish, and a release cover arranged above the blocking net; Preferably, the mesh number of the barrier net is not less than 80 meshes; Preferably, the release cover is provided with a vent hole.

6. The indoor population propagation method according to any one of claims 1 to 5, characterized in that: The raising period in step (1) is 40-45 days.

7. The indoor population propagation method according to any one of claims 1 to 6, characterized in that: Among the tobacco beetle parasitic wasps introduced in step (2), the ratio of wingless adult bees to winged adult bees is (7-9):

1.

8. The indoor population propagation method according to claim 5, characterized in that: After the step (2) of inoculating the tobacco beetle parasitic wasp for breeding, the method further includes placing a cotton ball soaked in honey water on top of the barrier net; Preferably, in the honey water, the volume ratio of honey to water is 1:(8-12); Preferably, the cotton ball is replaced every 1-2 days.

9. The indoor population propagation method according to any one of claims 1 to 8, characterized in that: The feeding time in step (2) is 12-15 days.

10. The indoor population propagation method according to claim 5, characterized in that: Before the release in step (2), the method also includes removing the blocking net and the release cover of the breeding device and placing the breeding device at the release location.

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