29 results about "Genetically modified wheat" patented technology

This invention relates to a powdery mildew resistance gene PmAeu1 derived from *Aegilops spp.*, its molecular markers, and applications, belonging to the field of genetic engineering. This invention utilizes the powdery mildew-resistant *Aegilops spp.* strain AE 1617 and the powdery mildew-susceptible strain AE 538 to construct mapping populations. Through BSR-Seq, fine genetic mapping, third-generation genome sequencing, and transgenic verification, a new powdery mildew resistance gene, PmAeu1, was cloned. This invention has demonstrated through transgenic wheat research that the PmAeu1 gene can significantly improve wheat resistance to powdery mildew. The *Aegilops spp.* powdery mildew resistance gene PmAeu1 can be applied to genetically improve wheat varieties to enhance wheat's powdery mildew resistance. This invention also develops a molecular marker, XAeuNLR1-2, for the PmAeu1 gene, which can be used to specifically detect the gene PmAeu1 as described in the claims and can be applied to marker-assisted breeding of the PmAeu1 gene.

The invention provides an application of a TaPMT-L gene in improving drought resistance of plants. A nucleotide sequence of the gene is shown as SEQ ID No.1; the invention also provides a specific plant breeding method. Compared with the prior art, the application has the following advantages: the wheat gene TaPMT-L is identified, and overexpression and RNAi interference expression vectors of the wheat gene TaPMT-L are constructed, so that a stably inherited TaPMT-L transgenic wheat material is obtained. Phenotypic and physiological analysis under drought stress treatment shows that the drought tolerance in a TaPMT-L overexpression strain is remarkably enhanced, and the drought tolerance in an RNAi silent strain is reduced, so that the TaPMT-L has a function of positively regulating and controlling the drought resistance in wheat. The drought-resistant regulatory gene which is clear in function and can be stably expressed provides a new molecular tool for genetic improvement of the drought-resistant character of wheat, and has important theoretical value and application prospect for cultivation of new varieties of drought-resistant wheat.

The invention belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease-resistant factor TaPKS26 in prevention and treatment of leaf rust. The CDS sequence of the encoding gene of the wheat disease-resistant factor TaPKS26 is shown as SEQ ID NO: 1, a transgenic wheat strain of the overexpressed gene TaPKS26 is obtained by adopting an agrobacterium-mediated method, and the transgenic wheat strain of the overexpressed gene TaPKS26 is proved to have enhanced resistance to wheat leaf rust compared with Fielder wheat after being infected with physiological races THT and PHT, and the wheat leaf rust resistance is improved. The expression quantity of the disease-resistant related marker gene is up-regulated, which indicates that the wheat disease-resistant factor TaPKS26 plays a positive regulation role in wheat leaf rust-resistant defensive reaction. The invention provides new gene resources and technical thoughts for the cultivation of leaf rust resistant varieties.

The TaSL1 protein or biological material provided by this invention has the function of increasing the number of grains per ear and the length of the ear in wheat. After reducing the expression of TaSL1 protein in wheat, the transgenic wheat obtained has a significantly increased number of grains per ear, ear length, and awn length compared with the control. The TaSL1 protein or biological material provides a new genetic resource for breeding wheat varieties with high grain count and long ears, which is of great significance for improving wheat yield.

The invention discloses an application of an elytrigia elongata gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. The nucleotide sequence of the gene Glu-1Ex is as shown in SEQ ID No.1. The invention also discloses an application of the gene Glu-1Ex in regulating and controlling the content of wheat protein and glutelin. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ex gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ex gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The analysis on the protein content of transgenic wheat proves that the Glu-1Ex gene of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the elytrigia elongata Glu-1Ex gene.

Triticale cultivar APT1415735, an awnless, winter variety with good plant height, silage yield, and straw strength is disclosed. The disclosure includes the seeds of triticale cultivar APT1415735, the plants of triticale APT1415735, and methods for producing a triticale plant produced by crossing the cultivar APT1415735 with itself or another triticale cultivar. The disclosure also relates to methods for producing a triticale plant containing in its genetic material one or more transgenes and to the transgenic triticale plants and plant parts produced by those methods. Further disclosed are triticale varieties or breeding varieties and plant parts derived from triticale cultivar APT1415735, to methods for producing other triticale varieties, lines or plant parts derived from triticale cultivar APT1415735, and to the triticale plants, varieties, and their parts derived from the use of those methods. The disclosure further relates to hybrid triticale seeds and plants produced by crossing the cultivar APT1415735 with another triticale cultivar.

The application relates to the technical field of genetic engineering, and discloses application of a transcription factor TabHLH168 protein in wheat disease resistance. The amino acid sequence of the TabHLH168 protein is shown as SEQ ID NO. 1; the coding gene sequence of the TabHLH168 protein is shown as SEQ ID NO. 2. The TabHLH168 protein is a target protein of a false smut fungus effect protein FpE02. The application uses the above-mentioned application of the transcription factor TabHLH168 protein in wheat disease resistance, overexpresses the coding gene of the TabHLH168 protein, obtains transgenic wheat with stable heredity and wheat stem base rot resistance, provides a new solution for carrying out wheat disease resistance breeding work, and has important significance in the field of wheat breeding with disease resistance.

A wheat transgenic plant carrying restorer of fertility genes specific to T. timopheevii CMS cytoplasm.

The invention relates to the technical field of plant gene transgenosis, in particular to a TaCML15 gene and application of the TaCML15 gene in cultivation of aphid-resistant transgenic wheat. According to the TaCML15 gene, the CDS sequence of the TaCML15 gene is as shown in SEQ ID NO. 1; according to the application of the TaCML15 gene, through overexpression of the TaCML15 gene, the application of improving aphid resistance of wheat or the application of cultivating aphid-resistant transgenic wheat is achieved. In the invention, a TaCML15 overexpression transgenic line shows improvement on insect resistance compared with a wild type, which indicates that the wheat gene TaCML15 participates in insect resistance regulation and control of plants, the discovery of the gene can provide gene resources for crop breeding, and the gene has a relatively wide application prospect.

The invention relates to a TaPIP5K-B1 gene for improving the grain weight of wheat grains as well as a CAPS marker and application of the TaPIP5K-B1 gene. The nucleotide sequence of the TaPIP5K-B1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoding protein of the TaPIP5K-B1 gene is as shown in SEQ ID NO. 2. The invention further provides a molecular marker CAPS-TaPIP5K-B1 of the gene of the TaPIP5K-B1, and the molecular marker of the gene of the TaPIP5K-B1 is The invention discovers and discloses a novel wheat TaPIP5K-B1 gene for the first time, and the gene can effectively increase wheat grains and increase the thousand grain weight of the wheat grains. Research on the gene finds that a transgenic wheat strain overexpressed with the TaPIP5K-B1 gene has a phenotype of thousand seed weight increase, so that the gene can be applied to improvement of important agronomic characters such as the size and thousand seed weight of seeds, the yield of crops is further increased, and the gene has important economic value and social benefit.

The application discloses application of a wheat TaWRKY69 gene in regulation of resistance to wheat stem base rot, and a broad-spectrum disease-resistant wheat protein TaWRKY69 is cloned from a wheat germplasm Shiyou 17 resistant to stem base rot. The TaWRKY69 gene of wheat is silenced by using a VIGS technology, and the result shows that silencing of the TaWRKY69 gene reduces the resistance of wheat to wheat stem base rot. A wheat with the TaWRKY69 gene is obtained by using an agrobacterium-mediated genetic transformation method, and the wheat with the TaWRKY69 gene enhances the resistance of wheat to stem base rot, which indicates that the TaWRKY69 gene positively regulates and improves the resistance of wheat to stem base rot. The TaWRKY69 protein can regulate the disease resistance, stem base rot resistance and false smut resistance of wheat, and can be applied to wheat breeding or assisted breeding.

The invention relates to application of a TaGRF2-A gene in enhancing heat resistance of wheat, and belongs to the field of plant genetic engineering. Specifically, the wheat TaGRF2-A gene participating in positive regulation and control of high-temperature stress resistance is cloned in wheat, so that the wheat TaGRF2-A gene is overexpressed, and compared with a wild plant, a wheat plant with the overexpressed TaGRF2-A gene shows higher high-temperature resistance; the TaGRF2-A gene enhances the tolerance of wheat under a high-temperature condition in manners of regulating the stomatal conductance of plants, optimizing the photosynthesis efficiency, improving the water utilization efficiency and the like, and a new genetic engineering modification means is provided for cultivating a new wheat variety with high yield, stable yield and high temperature resistance.

The present invention relates to a method for producing 1B or 1D omega-5 gliadin-deleted wheat and wheat produced thereby, and, specifically, to a method for producing and selecting 1B or 1D omega-5 gliadin gene-deleted wheat by using radiation. The method for producing 1B or 1D omega-5 gliadin-deleted wheat, according to the present invention, enables the production of a radiation breeding-based non-GMO mutant wheat or wheat breeding parental line while retaining agronomic traits similar to those of a wild-type wheat variety, and thus wheat with reduced toxicity to wheat-dependent exercise-induced anaphylaxis (WDEIA) can be produced for use in the production of processed wheat foods and the like, or a parental line for breeding wheat varieties with reduced toxicity to WDEIA can be produced.

The application belongs to the technical field of biological gene engineering, and particularly relates to a wheat salt-tolerant gene TaSGT1 and application thereof. The application provides a wheat salt-tolerant gene TaSGT1 and a plant overexpression vector containing TaSGT1 the gene. The wheat salt-tolerant gene TaSGT1 has a nucleotide sequence as shown in SEQ ID No. 1-SEQ ID No. 3, or a nucleotide sequence homologous to any of the above-mentioned nucleotide sequences with 75% or above, and in addition, the application further provides application of the salt-tolerant gene TaSGT1 in breeding of wheat varieties with salt-tolerant, drought-tolerant, early-maturing and dwarf traits. Experiments prove that the transgenic wheat provided by the application has obviously improved salt-tolerant and drought-tolerant capacity, early heading stage and reduced plant height.

The application discloses a wheat disease-resistant protein TaCNGC-Ps1 and a gene and application thereof, and belongs to the technical field of genetic engineering. TaCNGC-Ps1 The application discloses a wheat disease-resistant protein TaCNGC-Ps1 and a gene and application thereof, and belongs to the technical field of genetic engineering. The application discloses a wheat disease-resistant protein TaCNGC-Ps1 and a gene and application thereof, and belongs to the technical field of genetic engineering.

The present application belongs to the technical field of agricultural bioengineering, and relates to application of a wheat disease resistance factor TaPKS26 in leaf rust prevention and treatment. The CDS sequence of the coding gene of the wheat disease resistance factor TaPKS26 is shown as SEQ ID NO:1, and the present application adopts an agrobacterium-mediated method to obtain a transgenic wheat strain overexpressing the gene TaPKS26. After being infected with the physiological race THT and PHT of leaf rust, the transgenic wheat strain overexpressing the gene TaPKS26 is compared with Fielder wheat, and the results show that the resistance of the transgenic wheat strain to wheat leaf rust is enhanced, and the expression amount of a disease resistance related marker gene is up-regulated, indicating that the wheat disease resistance factor TaPKS26 plays a positive regulation role in the defense response of wheat against leaf rust. The present application provides a new gene resource and technical idea for breeding of a leaf rust disease resistance variety.

The present application relates to the technical field of genetic engineering, and particularly relates to application of wheat TaLNUE protein in regulating low nitrogen stress tolerance of plants. The present application designs single-stranded guide RNA targeting wheat TaLNUE gene, constructs an expression vector for knocking out TaLNUE gene, and transforms the expression vector into wild-type wheat to obtain transgenic wheat with inhibited TaLNUE gene function. Research shows that inhibiting expression of wheat TaLNUE protein can increase ear length and spikelet number of wheat under low nitrogen stress, thereby increasing single plant yield and total yield of wheat under low nitrogen stress, and improving nitrogen absorption efficiency and utilization efficiency of wheat under low nitrogen stress. The technical scheme of the present application provides a feasible method for improving wheat yield under low nitrogen stress by using genetic engineering technology.

Triticale cultivar NS16T0209, an awnless, winter variety with good plant height, silage yield, disease resistance and straw strength is disclosed. The disclosure includes the seeds of triticale cultivar NS16T0209, the plants of triticale NS16T0209, and methods for producing a triticale plant produced by crossing the cultivar NS16T0209 with itself or another triticale cultivar. The disclosure also relates to methods for producing a triticale plant containing in its genetic material one or more transgenes and to the transgenic triticale plants and plant parts produced by those methods. Further disclosed are triticale varieties or breeding varieties and plant parts derived from triticale cultivar NS16T0209, to methods for producing other triticale varieties, lines or plant parts derived from triticale cultivar NS16T0209, and to the triticale plants, varieties, and their parts derived from the use of those methods. The disclosure further relates to hybrid triticale seeds and plants produced by crossing the cultivar NS16T0209 with another triticale cultivar.

This application discloses the application of the PPR protein TaPPR13 and its encoding gene in regulating drought resistance in wheat. Specifically, it discloses the application of the protein with the amino acid sequence SEQ ID No. 1 and its encoding gene in regulating plant drought resistance. This application utilizes wheat-derived... TaPPR13 Genes were introduced into recipient plants, resulting in transgenic plants. TaPPR13 Homozygous plants of the gene. Experiments showed that, compared with the non-transgenic recipient control, transgenic wheat exhibited stronger tolerance to drought stress, with significantly higher plant survival rates and superior growth compared to the recipient control. Transgenic wheat was successfully grown under controlled water conditions in the field. TaPPR13 Genetically modified wheat exhibits better grain traits and higher plot yields, indicating TaPPR13 Overexpression of genes can significantly improve the drought resistance of plants. The drought resistance gene regulated in this application is of great significance and application value for breeding new drought-resistant plant varieties.

This invention relates to the field of plant biotechnology, specifically to the wheat TaRPM1-1A gene and its application in improving plant salt tolerance. TaRPM1-1A The nucleotide sequence of the gene is shown in SEQ ID NO:1. This invention will... TaRPM1-1A Functional studies were conducted on gene overexpression transfected into Fielder wheat, and the results showed that overexpression... TaRPM1-1A Genetically modified wheat exhibits significantly improved salt tolerance compared to Fielder wheat, therefore TaRPM1-1A Genes play a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and are of great significance for future wheat salt tolerance breeding and production.

The invention discloses application of a wheat disease-resistant factor TaC4H1 in prevention and treatment of brown leaf rust, and belongs to the technical field of genetic engineering. The coding gene CDS sequence of the wheat disease resistance factor TaC4H1 is as shown in SEQ ID NO: 1, and the amino acid sequence is as shown in SEQ ID NO: 2. According to the invention, a transgenic wheat strain capable of overexpressing TaC4H1 is obtained by adopting an agrobacterium-mediated method, and the condition that the wheat disease-resistant factor TaC4H1 plays a positive regulation role in wheat leaf rust resistance immune response is known by comparing the morbidity condition of transgenic wheat and wild Fielder wheat after being infected with physiological races of leaf rust THT and PHT and the expression condition of a disease-resistant related marker gene. On the basis, the wheat disease-resistant factor TaC4H1 can be used for creating a leaf rust-resistant wheat material, so that a gene resource and a technical thought are provided for leaf rust-resistant breeding of wheat.

The application discloses application of a wheat disease resistance factor TaC4H1 in leaf rust prevention and belongs to the technical field of genetic engineering. The coding gene CDS sequence of the wheat disease resistance factor TaC4H1 is shown in SEQ ID NO:1, and the amino acid sequence is shown in SEQ ID NO:2. The application obtains a transgenic wheat strain capable of overexpressing TaC4H1 by adopting an agrobacterium-mediated method TaC4H1 By comparing the disease incidence and the expression of disease resistance related marker genes of the transgenic wheat and wild type Fielder wheat after infection of leaf rust THT and PHT physiological races, it is known that the wheat disease resistance factor TaC4H1 plays a positive regulation role in the immune response of the wheat against the leaf rust. Based on this, the wheat disease resistance factor TaC4H1 can be used for creating a wheat material resistant to the leaf rust, which provides a gene resource and a technical idea for the breeding of the wheat resistant to the leaf rust.

The invention discloses application of a wheat TaWRKY69 gene in regulation and control of wheat stem rot resistance, according to the application, a broad-spectrum disease-resistant wheat protein TaWRKY69 is separated and cloned from stem rot resistant wheat germplasm Shiyou 17, the TaWRKY69 gene of wheat is silenced by adopting a VIGS technology, and the result shows that the resistance of wheat to the wheat stem rot is reduced through silencing of the TaWRKY69 gene, so that the wheat stem rot resistance is improved, and the wheat stem rot resistance is improved. TaWRKY69 transgenic wheat is obtained by adopting an agrobacterium tumefaciens-mediated genetic transformation method, the TaWRKY69 transgenic wheat enhances the resistance of the wheat to the stem rot, the result shows that the positive regulation of the TaWRKY69 gene improves the resistance of the wheat to the stem rot, and the TaWRKY69 protein can regulate the disease resistance, the stem rot resistance and the fusarium pseudograminearum resistance of the wheat and can be applied to wheat breeding or auxiliary breeding.

The invention discloses a Glu-1Ey gene for regulating and controlling wheat protein content, a protein and application of the Glu-1Ey gene. The nucleotide sequence of the Glu-1Ey gene is shown as SEQ ID No. 1. According to the invention, an overexpression vector for transforming the agropyron elongatum Glu-1Ey gene is successfully constructed by using a gene overexpression technology, and a strain for overexpressing the Glu-1Ey gene is obtained by using an agrobacterium-mediated wheat immature embryo genetic transformation method. The protein content analysis of transgenic wheat proves that the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata can improve the protein content and glutelin content of wheat. The technical scheme provided by the invention has important significance for obtaining high-quality wheat new germplasm by utilizing the high-molecular-weight glutelin y-type subunit gene Glu-1Ey of elytrigia elongata.

The invention discloses a wheat heading stage regulation gene and application, and relates to the technical field of functional genes, the wheat heading stage regulation gene TaWRKY75-2D has a nucleotide sequence as shown in SEQ ID NO: 1, and the application of the gene TaWRKY75-2D as a target in at least one of the following aspects: regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage, regulating the wheat heading stage and regulating the wheat heading stage. And improving agronomic characters of the wheat heading stage or screening wheat varieties with advanced heading stages. By creating a TaWRKY75-2D overexpression transgenic line, it is found that overexpression of the gene causes advanced heading of transgenic wheat, and further through molecular biological analysis, it is found that the gene can regulate and control the heading period of wheat by combining with a circadian rhythm pathway gene LHY.

This invention discloses a wheat heading date regulating gene and its application, relating to the field of functional gene technology. The wheat heading date regulating gene, TaWRKY75-2D, has the nucleotide sequence shown in SEQ ID NO: 1. The TaWRKY75-2D gene is used as a target in at least one of the following applications: regulating wheat heading date, improving wheat heading date agronomic traits, or screening for wheat varieties with earlier heading dates. This invention, through the creation of TaWRKY75-2D overexpression transgenic lines, found that overexpression of this gene caused earlier heading in transgenic wheat. Further molecular biological analysis revealed that this gene can regulate wheat heading date by binding to the circadian rhythm pathway gene LHY.

The invention discloses application of TaDJA protein and a coding gene thereof in improving salt stress resistance of wheat, and belongs to the technical field of gene engineering. According to the invention, the salt tolerance of the TaDJA gene is verified on Fielder wheat. Experimental results prove that the development of TaDJA transgenic wheat is basically consistent with that of a wild type plant under normal culture conditions; under the salt stress condition of 150 mmol / L, the damage degree of wheat leaves and roots of the high-expression TaDJA gene is small, and the phenotypic characteristics of the wheat are superior to those of a wild type (the length and fresh weight of an overground part / an underground part). The invention provides an important gene resource for improving the salt tolerance of wheat, and has important application value for promoting the development of a wheat molecular breeding technology.

The present invention relates to a method for producing 33-mer alpha-gliadin-deleted wheat and wheat produced thereby and, specifically, to a method for producing α-D4-1 and α-D4-2 genes-deleted wheat by using radiation and selecting same. The method for producing 33-mer alpha-gliadin-deleted wheat, according to the present invention, enables the production of a mutant wheat or wheat breeding parental line, which is a radiation breeding-based non-GMO, while having agronomic traits similar to those of a wild-type wheat variety, and thus wheat with reduced toxicity to celiac disease can be produced, for use in the production of processed wheat foods and the like, or a parental line for wheat breeding varieties with reduced toxicity to celiac disease can be produced.